检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

酢浆草属12种植物核型分析

西北植物学报 2024年第3期44卷 502-510页

作者：常健超陈生煜林吴白羽唐小云翟俊文吴沙沙福建农林大学风景园林与艺术学院福州350002 福建省观赏植物种植资源创新与应用工程技术研究中心福州350002

【目的】为了从细胞学角度明确酢浆草属(Oxalis)12种植物核型特征与亲缘关系,探究酢浆草属植物染色体的多样性,进一步为酢浆草种质资源鉴定与杂交育种亲本选择提供参考。【方法】应用根尖压片法对酢浆草属12个物种植物有丝分裂中期的染... 详细信息

【目的】为了从细胞学角度明确酢浆草属(Oxalis)12种植物核型特征与亲缘关系,探究酢浆草属植物染色体的多样性,进一步为酢浆草种质资源鉴定与杂交育种亲本选择提供参考。【方法】应用根尖压片法对酢浆草属12个物种植物有丝分裂中期的染色体形态、数目及其核型进行观察分析。【结果】(1)7个物种的染色体数目为首次报道,5种已报道物种中,兔耳酢浆草(***)和黄花酢浆草(***-caprae)染色体数目与前人报道一致,其余3种,与先前报道存在差异,其中2n=26为酢浆草属植物中鲜见报道的染色体数目,共发现7种染色体基数,其中x=13鲜见报道,倍性范围从2x~6x;实际染色体大小范围为0.27~2.23μm,着丝点位置为中部着丝点区(m)和亚中部着丝点区(sm),核型类型共4种,核型不对称系数范围为56.31%~65.40%。(2)12种酢浆草属植物中兔耳酢浆草最为进化,扁平酢浆草(***)最为原始。(3)根据染色体核型相似性可将酢浆草属12种植物划分为4组。【结论】酢浆草属12个物种具有广泛的核型多样性,核型分类结果与前人形态学分类不完全一致。

关键词：酢浆草属细胞学核型染色体进化亲缘关系

同方期刊数据库

14种酢浆草属植物花粉形态及其亲缘关系分析

在线全文

学校读者我要写书评

暂无评论

广西植物 2024年第4期44卷 682-698页

作者：陈生煜唐小云肖云韩茹翟俊文吴沙沙福建农林大学风景园林与艺术学院福州350002

利用扫描电镜对14种酢浆草属植物花粉形态及表面纹饰进行观测,对其花粉形态进行聚类分析,并基于孢粉学聚类结果,探讨其种间亲缘关系。结果表明:(1)所观测的14种植物花粉粒为近球形或扁球形,极面观均为三裂圆形,多数为3沟花粉粒,稀2沟(... 详细信息

利用扫描电镜对14种酢浆草属植物花粉形态及表面纹饰进行观测,对其花粉形态进行聚类分析,并基于孢粉学聚类结果,探讨其种间亲缘关系。结果表明:(1)所观测的14种植物花粉粒为近球形或扁球形,极面观均为三裂圆形,多数为3沟花粉粒,稀2沟(星星酢浆草)和4沟(黄花酢浆草);其中,96.3%花粉粒为中等大小,3.7%为小花粉粒;不同花柱类型都表现为长雄蕊花粉比短雄蕊花粉大;外壁纹饰可分为复网状和网状两类。(2)首次发现3种存在异常花粉粒,分别为桃之辉酢浆草、一片心酢浆草及黄花酢浆草,花粉的异常表现为萌发沟数目和排列异常,其外壁纹饰结构与正常花粉粒未有明显不同。(3)根据外壁纹饰可将14种划分为2大类。一类为复网状花粉,仅构巢酢浆草和果香酢浆草为该类纹饰,二者亲缘关系较近。另一类为网状花粉,对12种具网状花粉酢浆草进行聚类分析可划分为4个类型,其中桃之辉酢浆草、双色冰淇淋酢浆草、藤双色冰淇淋酢浆草亲缘关系较近;扁平酢浆草、黄花酢浆草、纳马夸纳酢浆草及兔耳酢浆草亲缘关系较近;大花酢浆草、星星酢浆草、乳白蝴蝶叶酢浆草亲缘关系较近;吉氏酢浆草和一片心酢浆草与其他酢浆草亲缘关系较远。该研究结果表明,花粉形态相近的物种在植株形态上有一定的相似性,花粉形态特征可作为种间亲缘关系研究和分类划分依据,进一步为酢浆草属植物杂交育种亲本选择提供理论基础。

关键词：酢浆草属花粉形态异型花柱异常花粉亲缘关系

阳桃(Averrhoa carambola)实时荧光定量PCR内参基因的筛选与验证

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2024年第3期22卷 789-799页

作者：李笑平朱雅婷赵亚梅陈蕾任惠吴沙沙翟俊文福建农林大学园林学院兰科植物保护与利用国家林业和草原局重点实验室福州350002 广西农业科学院园艺研究所南宁530007

阳桃(Averrhoa carambola)具有很高的营养、药用及观赏价值,但功能基因组方面的研究基础较为薄弱。为使阳桃相关功能基因表达分析的定量实验结果更加准确,需要选择稳定性好的内参基因。然而目前还没有关于阳桃内参基因筛选的报道。本研... 详细信息

阳桃(Averrhoa carambola)具有很高的营养、药用及观赏价值,但功能基因组方面的研究基础较为薄弱。为使阳桃相关功能基因表达分析的定量实验结果更加准确,需要选择稳定性好的内参基因。然而目前还没有关于阳桃内参基因筛选的报道。本研究基于阳桃基因组及转录组数据,选取了α-TUB、β-TUB、Actin、UBQ、UBC4、EF1α和H2B共7个管家基因作为候选内参基因;利用实时荧光定量PCR(RT-qPCR)技术检测各候选内参基因在茎、叶、果、花序芽、叶芽及不同时期花序中的表达情况。使用软件geNorm、Normfinder和BestKeeper综合评估了候选内参基因的稳定性。结果表明,α-TUB和β-TUB在阳桃不同器官中稳定性最好,β-TUB和UBC4在阳桃不同时期花序中稳定性最好。此外,通过分析成花基因AP1(APETALA1)和开花抑制因子TFL1(TERMINAL FLOWER 1)的表达模式,进一步验证了内参基因筛选结果的可靠性。本研究筛选得到的稳定内参基因为后期开展阳桃相关功能基因表达分析的研究提供了基础。

关键词：阳桃内参基因实时荧光定量PCR 表达稳定性

Phylogenetic estimation and morphological evolution of Alsineae(Caryophyllaceae)shed new insight into the taxonomic status of the genus Pseudocerastium

在线全文

学校读者我要写书评

暂无评论

Plant Diversity 2021年第4期43卷 299-307页

作者： Gang Yao Bine Xue Kun Liu Yuling Li Jiuxiang Huang junwen zhai College of Forestry and Landscape Architecture South China Agricultural UniversityGuangzhou510642China College of Horticulture and Landscape Architecture Zhongkai University of Agriculture and EngineeringGuangzhou510225GuangdongChina College of Life Sciences Anhui Normal UniversityWuhu241000AnhuiChina College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou350002FujianChina

Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to *** genus has been widely accepted since it was described in 1998,however its phylogenetic position within Caryophyllaceae has never been *** the present study,the whole plastid genome and nuclear ribosomal internal transcribed spacer(ITS)sequences of Pseudocerastium stellarioides was obtained through genome skimming,and the phylogenetic position of the species was studied for the first *** phylogenomic analysis of Caryophyllaceae revealed that Pseudocerastium is clustered within the tribe Alsineae with strong *** analyses based on an enlarged taxon sampling of Alsineae using five DNA regions(matK,rbcL,rps16 intron,trnL-F and ITS)revealed that *** was nested deeply within Cerastium with strong *** of morphological character evolution suggest that the ancestral states in Alsineae include three styles and a six-lobed capsule at the apex,while both Cerastium and Pseudocerastium have five styles and ten lobes at the apex of the capsule,further supporting their close *** species Pseudocerastium stellarioides is similar to Cerastium wilsonii in morphology,but differs in having villous indumentum on the lower part of the filaments and compressed globose ***,based on the present molecular and morphological evidence,the generic name Pseudocerastium is reduced here as a new synonym of Cerastium and the species *** is transferred to Cerastium as ***.

关键词： Alsineae Cerastium Character evolution Genome skimming Molecular phylogeny Taxonomy

A review for the breeding of orchids: Current achievements and prospects

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2021年第5期7卷 380-392页

作者： Chengru Li Na Dong Yamei Zhao Shasha Wu Zhongjian Liu junwen zhai Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhouChina

Orchids are horticultural plants with significant ornamental and economic *** market circulation and rising trade in orchids is forcing breeders to develop varieties with unique characteristics,including flower color,morphology,and resistance using a range of approaches,including traditional and molecular *** in high-throughput technologies have generated extensive data sets with greater sequencing depths and broader coverage,providing the potential for discovering new genes/pathways that give rise to key *** attempts have been made to use emerging molecular and omics methods to accelerate the breeding process in certain of the commercially valuable *** review consolidates current approaches and achievements in orchid breeding and discusses their future applications for improving the resistance,ornamental,and other valuable characteristics of these plants.

关键词： Orchidaceae Multi-omics Breeding methods

Comparative analysis of plastomes in Oxalidaceae:Phylogenetic relationships and potential molecular markers

在线全文

学校读者我要写书评

暂无评论

Plant Diversity 2021年第4期43卷 281-291页

作者： Xiaoping Li Yamei Zhao Xiongde Tu Chengru Li Yating Zhu Hui Zhong Zhong-Jian Liu Shasha Wu junwen zhai Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou 350002China

The wood sorrel family,Oxalidaceae,is mainly composed of annual or perennial herbs,a few shrubs,and trees distributed from temperate to tropical *** of Oxalidaceae are of high medicinal,ornamental,and economic *** the rich diversity and value of Oxalidaceae,few molecular markers or plastomes are available for phylogenetic analysis of the ***,we reported four new whole plastomes of Oxalidaceae and compared them with plastomes of three species in the family,as well as the plastome of Rourea microphylla in the closely related family *** eight plastomes ranged in length from 150,673 bp(Biophytum sensitivum)to 156,609 bp(***).Genome annotations revealed a total of 129e131 genes,including 83e84 protein-coding genes,eight rRNA genes,37 tRNA genes,and two to three *** analyses showed that the plastomes of these species have minor variations at the gene *** smaller plastomes of herbs *** and three Oxalis species are associated with variations in IR region sizes,intergenic region variation,and gene or intron *** identified sequences with high variation that may serve as molecular markers in taxonomic studies of *** phylogenetic trees of selected superrosid representatives based on 76 protein-coding genes corroborated the Oxalidaceae position in Oxalidales and supported it as a sister to *** research also supported the monophyly of the COM(Celastrales,Oxalidales,and Malpighiales)clade.

关键词： Oxalidaceae Plastome Oxalidales Gene loss COM clade Phylogeny

Nuclear phylogeny and insights into wholegenome duplications and reproductive development of Solanaceae plants

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2023年第4期4卷 301-322页

作者： Jie Huang Weibin Xu junwen zhai Yi Hu Jing Guo Caifei Zhang Yiyong Zhao Lin Zhang Christopher Martine Hong Ma Chien-Hsun Huang State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development Ministry of Education Key Laboratory of Biodiversity and Ecological EngineeringInstitute of Plant BiologyCenter of Evolutionary BiologySchool of Life SciencesFudan UniversityShanghai 200438China Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain Guangxi Institute of BotanyGuangxi Zhuangzu Autonomous Region and Chinese Academy of SciencesGuilin 541006China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology the Huck Institutes of Life Sciencesthe Pennsylvania State UniversityUniversity ParkState CollegePA 16802USA Department of Biology Bucknell UniversityLewisburgPA 17837USA

Solanaceae,the nightshade family,have2700 species,including the important crops potato and tomato,ornamentals,and medicinal *** sequenced Solanaceae genomes show evidence for wholegenome duplication(WGD),providing an excellent opportunity to investigate WGD and its ***,we generated 93 transcriptomes/genomes and combined them with 87 public datasets,for a total of 180 Solanaceae species representing all four subfamilies and 14 of 15 *** 1700 nuclear genes from these transcriptomic/genomic datasets were used to reconstruct a highly resolved Solanaceae phylogenetic tree with six major *** Solanaceae tree supports four previously recognized subfamilies(Goetzeioideae,Cestroideae,Nicotianoideae,and Solanoideae)and the designation of three other subfamilies(Schizanthoideae,Schwenckioideae,and Petunioideae),with the placement of several previously unassigned *** placed a Solanaceae-specific whole-genome triplication(WGT1)at81 million years ago(mya),before the divergence of Schizanthoideae from other Solanaceae subfamilies at73 *** addition,we detected two gene duplication bursts(GDBs)supporting proposed WGD events and four other *** investigation of the evolutionary histories of homologs of carpel and fruit developmental genes in 14 gene(sub)families revealed that 21 gene clades have retained gene *** were likely generated by the Solanaceae WGT1 and may have promoted fleshy fruit *** study presents a well-resolved Solanaceae phylogeny and a new perspective on retained gene duplicates and carpel/fruit development,providing an improved understanding of Solanaceae evolution.

关键词： Solanaceae phylogeny transcriptome carpel and fruit development whole-genome duplication

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

印缅钗子股,中国兰科一新记录种

福建农林大学学报（自然科学版） 2022年第3期51卷 416-418页

作者：马良翟俊文柳明珠兰思仁福建农林大学园林学院福建农林大学林学院福建福州350002

报道了中国兰科钗子股属1新记录种——印缅钗子股(Luisia zeylanica Lindl.),并提供特征描述及彩色图片.凭证标本现存于福建农林大学林学院树木标本室(FJFC).

关键词：中国兰科新记录种印缅钗子股

在线全文

学校读者我要写书评

暂无评论

台湾独蒜兰假鳞茎显微及超微结构观察

西北植物学报 2022年第2期42卷 242-254页

作者：吴春梅秦思吴梅翟俊文吴沙沙福建农林大学园林学院兰科植物保护与利用国家林业与草原局重点实验室福州350002 金华市农业科学研究院浙江金华321007

以休眠期的台湾独蒜兰(Pleione formosana)假鳞茎为试材,利用石蜡切片及透射电子显微镜,分别对假鳞茎的5个部位(假鳞茎基部、假鳞茎中部、假鳞茎外侧、假鳞茎与叶芽相接处、假鳞茎与花芽相接处)进行了系统观察分析。结果发现:(1)台湾独... 详细信息

以休眠期的台湾独蒜兰(Pleione formosana)假鳞茎为试材,利用石蜡切片及透射电子显微镜,分别对假鳞茎的5个部位(假鳞茎基部、假鳞茎中部、假鳞茎外侧、假鳞茎与叶芽相接处、假鳞茎与花芽相接处)进行了系统观察分析。结果发现:(1)台湾独蒜兰假鳞茎5个部位均观察到液泡及不同程度液泡化的细胞,表皮细胞覆着有厚厚的蜡质层。(2)台湾独蒜兰假鳞茎基部、中部、外侧细胞中均存在一定的叶绿体,少量线粒体分布在其周围。(3)假鳞茎薄壁细胞中存在淀粉粒及造粉体,且造粉体伴随有形成淀粉粒的现象。(4)假鳞茎基部、中部、外侧及其与花芽相接处的薄壁细胞壁之间有大量胞间连丝,筛管-伴胞复合体与薄壁细胞间也存在胞间连丝,同时细胞间存在不同形状的细胞间隙。研究结果表明,台湾独蒜兰假鳞茎发挥了其作为储水器官、光合作用场所及储存碳水化合物的功能,同时休眠期间主要以共质体途径进行物质的交换与运输。

关键词：台湾独蒜兰假鳞茎显微结构超微结构贮藏同化物途径