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Microglial Exosome miR-7239-3p Promotes Glioma Progression by Regulating Circadian Genes

Neuroscience Bulletin 2021年第4期37卷 497-510页

作者： Xuepei Li junwen guan Zhou Jiang Shuting Cheng Wang Hou Junjie Yao Zhengrong Wang Ministry of Health Key Laboratory of Chronobiology College of Basic Medicine and Forensic MedicineSichuan UniversityChengdu 610041China Medical Simulation Center Chengdu First People’s HospitalChengdu 610041China Neurosurgery Department West China HospitalSichuan UniversityChengdu 610041China Department of Respiratory and Critical Care Medicine West China HospitalSichuan UniversityChengdu 610041China Department of Anesthesiology Wuhan Third HospitalTongren Hospital of Wuhan UniversityWuhan 410000China

Glioma-associated microglial cells,a key component of the tumor microenvironment,play an important role in glioma progression.In this study,the mouse glioma cell line GL261 and the mouse microglia cell line BV2 were chosen.First,circadian gene expression in glioma cells co-cultured with either M1 or M2 microglia was assessed and the exosomes of M2-polarized and unpolarized BV-2 microglia were extracted.Subsequently,we labeled the exosomes with PKH67 and treated GL261 cells with them to investigate the exosome distribution.GL261 cell phenotypes and related protein expression were used to explore the role of M2 microglial exosomes in gliomas.Then a specific miR-7239-3p inhibitor was added to verify miR-7239-3p functions.Finally,the mouse subcutaneous tumorigenic model was used to verify the tumorigenic effect of M2 microglial exosomes in vivo.Our results showed that in gliomas co-cultured with M2 microglia,the expression of the BMAL1 protein was decreased(P<0.01),while the expression of the CLOCK protein was increased(P<0.05);opposite results were obtained in gliomas co-cultured with M1 microglia.After treatment with M2 microglial exosomes,the apoptosis of GL261 cells decreased(P<0.001),while the viability,proliferation,and migration of GL261 cells increased.Increased expression of N-cadherin and Vimentin,and decreased E-cadherin expression occurred upon treatment with M2 microglial exosomes.Addition of an miR-7239-3p inhibitor to M2 microglial exosomes reversed these results.In summary,we found that miR-7239-3p in the glioma microenvironment is recruited to glioma cells by exosomes and inhibits Bmal1 expression.M2 microglial exosomes promote the proliferation and migration of gliomas by regulating tumor-related protein expression and reducing apoptosis.

关键词： Glioma Microglia Bmal1 Exosome miR-7239-3p

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Microstructure evolution, mechanical properties and fracture behavior of Al-xSi/AZ91D bimetallic composites prepared by a compound casting

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Journal of Magnesium and Alloys 2024年第5期12卷 1944-1964页

作者： guangyu Li Wenming Jiang Feng guan junwen Zhu Yang Yu Zitian Fan State Key Laboratory of Materials Processing and Die & Mould Technology School of Materials Science and Engineering Huazhong University of Science and Technology

In this paper, the effect of the Si content on microstructure evolution, mechanical properties, and fracture behavior of the Al-xSi/AZ91D bimetallic composites prepared by compound casting was investigated systematically. The obtained results showed that all the Al-xSi/AZ91D bimetallic composites had a metallurgical reaction layer（MRL）, whose thickness increased with increasing Si content for the hypoeutectic Al-Si/AZ91D composites, while the hypereutectic Al-Si/AZ91D composites were opposite. The MRL included eutectic layer（E layer）,intermetallic compound layer（IMC layer） and transition region layer（T layer）. In the IMC layer, the hypereutectic Al-Si/AZ91D composites contained some Si solid solution and flocculent Mg2Si+Al-Mg IMCs phases not presented in the hypoeutectic Al-Si/AZ91D composites.Besides, increasing Si content, the thickness proportion of the T layer increased, forming an inconsistent preferred orientation of the MRL.The shear strengths of the Al-xSi/AZ91D bimetallic composites enhanced with increasing Si content, and the Al-15Si/AZ91D composite obtained a maximum shear strength of 58.6 MPa, which was 73.4% higher than the Al-6Si/AZ91D composite. The fractures of the Al-xSi/AZ91D bimetallic composites transformed from the T layer into the E layer with the increase of the Si content. The improvement of the shear strength of the Al-xSi/AZ91D bimetallic composites was attributed to the synergistic action of the Mg2Si particle reinforcement, the reduction of oxidizing inclusions and the ratio of Al-Mg IMCs as well as the orientation change of the MRL.

关键词： Al/Mg bimetallic composites Si content Mg 2 Si reinforcement Microstructure Mechanical properties Fracture behavior

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华西医院对颅脑创伤治疗方法选择的经验

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中华创伤杂志 2016年第8期32卷 673-676页

作者：关俊文游潮四川大学华西医院神经外科成都610041

颅脑创伤目前已经成为青壮年死亡的主要原因，因颅脑创伤导致的死亡和伤残也成为社会和家庭巨大的负担。如何有效地救治颅脑创伤已经成为一个全球性的问题。世界卫生组织预测，到2020年颅脑创伤将超过其他疾病成为死亡和致残的主要因素... 详细信息

颅脑创伤目前已经成为青壮年死亡的主要原因，因颅脑创伤导致的死亡和伤残也成为社会和家庭巨大的负担。如何有效地救治颅脑创伤已经成为一个全球性的问题。世界卫生组织预测，到2020年颅脑创伤将超过其他疾病成为死亡和致残的主要因素。华西医院作为西部大型的医疗中心，

关键词：颅脑创伤华西医院治疗方法世界卫生组织医疗中心死亡青壮年

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Neutrophil diversity and plasticity:Implications for organ transplantation

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Cellular & Molecular Immunology 2023年第9期20卷 993-1001页

作者： junwen Qu Jingsi Jin Ming Zhang Lai guan Ng Shanghai Immune Therapy Institute Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of Urology Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China

Neutrophils,as the first defenders against external microbes and stimuli,are highly active and finely regulated innate immune cells.Emerging evidence has challenged the conventional dogma that neutrophils are a homogeneous population with a short lifespan that promotes tissue damage.Recent findings on neutrophil diversity and plasticity in homeostatic and disease states have centered on neutrophils in the circulation.In contrast,a comprehensive understanding of tissue-specialized neutrophils in health and disease is still lacking.This article will first discuss how multiomics advances have contributed to our understanding of neutrophil heterogeneity and diversification in resting and pathological settings.This discussion will be followed by a focus on the heterogeneity and role of neutrophils in solid organ transplantation and how neutrophils may contribute to transplant-related complications.The goal of this article is to provide an overview of the research on the involvement of neutrophils in transplantation,with the aim that this may draw attention to an underappreciated area of neutrophil research.

关键词： Neutrophil Heterogeneity Transplantation

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Improving mechanical properties of AZ91D magnesium/A356 aluminum bimetal prepared by compound casting via a high velocity oxygen fuel sprayed Ni coating

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Journal of Magnesium and Alloys 2022年第4期10卷 1075-1085页

作者： guangyu Li Wenming Jiang Feng guan junwen Zhu Yang Yu Zitian Fan State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

In this paper,a Ni coating was deposited on the surface of the A356 aluminum alloy by high velocity oxygen fuel spraying to improve the performance of the AZ91D magnesium/A356 aluminum bimetal prepared by a compound casting.The effects of the Ni coating as well as its thickness on microstructure and mechanical properties of the AZ91D/A356 bimetal were systematically researched for the first time.Results demonstrated that the Ni coating and its thickness had a significant effect on the interfacial phase compositions and mechanical properties of the AZ91D/A356 bimetal.The 10μm’s Ni coating cannot prevent the generation of the Al-Mg intermetallic compounds(IMCs)at the interface zone of the AZ91D/A356 bimetal,while the Ni coating with the thickness of 45μm and 190μm can avoid the formation of the Al-Mg IMCs.When the Ni coating was 45μm,the Ni coating disappeared and transformed into Mg-Mg_(2)Ni eutectic structures+Ni_(2)Mg_(3)Al particles at the interface zone.With a thickness of 190μm’s Ni coating,part of the Ni coating remained and the interface layer was composed of the Mg-Mg_(2)Ni eutectic structures+Ni_(2)Mg_(3)Al particles,Mg_(2)Ni layer,Ni solid solution(SS)layer,Al_(3)Ni_(2) layer,Al_(3)Ni layer and sporadic Al_(3)Ni+Al-Al_(3)Ni eutectic structures from AZ91D side to A356 side in sequence.The interface layer consisting of the Mg-Ni and Al-Ni IMCs obtained with the Ni coating had an obvious lower hardness than the Al-Mg IMCs.The shear strength of the AZ91D/A356 bimetal with a Ni coating of 45μm thickness enhanced 41.4%in comparison with that of the bimetal without Ni coating,and the fracture of the bimetal with 45μm’s Ni coating occurred between the Mg matrix and the interface layer with a mixture of brittle fracture and ductile fracture.

关键词： Magnesium/aluminum bimetal Microstructure Mechanical properties Ni coating Compound casting High velocity oxygen fuel spraying

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基于“一气周流,土枢四象”理论探讨稳定型心绞痛病机与治疗

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亚太传统医药 2024年第3期20卷 90-93页

作者：付晓婧姜钧文关正宇辽宁中医药大学研究生学院辽宁沈阳110847 辽宁中医药大学附属医院辽宁沈阳110032

“一气周流,土枢四象”理论出自黄元御《四圣心源》,强调中焦土气斡旋,左路木火升发,右路金水敛降,形成人体之圆运动。基于“一气周流”理论探讨稳定型心绞痛的病机,认为中气衰败是其发生的根本。治疗上,以补土健中、复其斡旋为核心,把... 详细信息

“一气周流,土枢四象”理论出自黄元御《四圣心源》,强调中焦土气斡旋,左路木火升发,右路金水敛降,形成人体之圆运动。基于“一气周流”理论探讨稳定型心绞痛的病机,认为中气衰败是其发生的根本。治疗上,以补土健中、复其斡旋为核心,把握肝、心、肺、肾四象气机的全局动态,采用燥湿暖水、升木达郁、降逆敛金等治法,调畅一身气机。

关键词：稳定型心绞痛一气周流黄元御中医病机

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基于增强现实理论的神经外科头颅体表定位系统

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生物医学工程学杂志 2019年第3期36卷 428-434页

作者：严洪立关俊文李永红张秋铭周义成李雪珮徐江龙四川大学华西医院神经外科成都610041 成都信息工程大学电子工程学院成都610225 宜宾职业技术学院四川宜宾644003

神经导航系统设备昂贵、操作复杂且普及率低,仅存于部分大型医疗机构。为了满足其他中小型医疗机构对于神经外科手术导航系统的需求,本文阐述了一种基于增强现实(AR)理论开发的头颅体表定位系统。该系统借鉴AR技术将虚拟世界影像与现实... 详细信息

神经导航系统设备昂贵、操作复杂且普及率低,仅存于部分大型医疗机构。为了满足其他中小型医疗机构对于神经外科手术导航系统的需求,本文阐述了一种基于增强现实(AR)理论开发的头颅体表定位系统。该系统借鉴AR技术将虚拟世界影像与现实世界影像相融合的思想,将患者头部计算机断层扫描成像(CT)或磁共振成像(MRI)与现实中患者头颅进行投影融合,实现头颅体表定位。文章重点讨论医学图像可视化中医学数字成像和通信(DICOM)国际标准、三维重建、AR图像图层融合等关键点。本研究测试表明,该系统适用于多款手机,能实现二维图像展示、三维重建渲染以及临床头颅体表定位应用,对于辅助神经外科手术头颅体表定位具有一定意义。

关键词：增强现实神经外科三维重建体表定位 Android

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胆囊收缩素诱导的急性胰腺炎对小鼠自由节律的影响研究

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生物医学工程学杂志 2016年第1期33卷 115-119,125页

作者：李永红杨小平郭盼盼刘延友严洪立李率真关俊文成都信息工程大学电子工程学院物理场生物效应及仪器四川高校重点实验室成都610225 四川大学卫生部时间生物学重点实验室成都610041 四川大学华西医院神经外科成都610041

本文研究了胆囊收缩素(CCK)诱导的急性胰腺炎对ICR小鼠自发性活动自由节律的影响,分析生物节律与炎症性疾病及急性胰腺炎病理机制的相互关系。通过对全黑状态下的转轮甄别活动相和休息相行CCK诱导的急性胰腺炎造模,对比造模后的炎症程... 详细信息

本文研究了胆囊收缩素(CCK)诱导的急性胰腺炎对ICR小鼠自发性活动自由节律的影响,分析生物节律与炎症性疾病及急性胰腺炎病理机制的相互关系。通过对全黑状态下的转轮甄别活动相和休息相行CCK诱导的急性胰腺炎造模,对比造模后的炎症程度以及两组前后周期、相位等节律指标的变化。结果发现相同剂量的急性胰腺炎ICR小鼠模型,休息相造模组比活动相造模组炎症程度重;且急性胰腺炎ICR小鼠活动节律的周期延长,在休息相造模组尤为明显。研究表明CCK诱导的急性胰腺炎可影响ICR小鼠的自由活动节律,且自由运转状态下不同相位造模时炎症轻重有明显区别,为急性胰腺炎重症化趋势的病理机制研究提供了可能的线索。

关键词：胆囊收缩素急性胰腺炎生物节律自由运转

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中国50百分位人体驾驶坐姿有限元建模及验证

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北京汽车 2024年第1期 1-7页

作者：黄俊文关炎芳李武杰吴心平河南工业大学机电工程学院河南郑州450001 美力科技股份有限公司浙江绍兴312599

建立中国50百分位人体驾驶坐姿有限元模型,完善中国人体有限元生物力学模型数据库,提供更加系统全面的人体生物力学模型验证方法。该模型基于THUMS (Total Human Model for Safety,虚拟人体模型)确定缩放比例,通过人体解剖学参数等数据... 详细信息

建立中国50百分位人体驾驶坐姿有限元模型,完善中国人体有限元生物力学模型数据库,提供更加系统全面的人体生物力学模型验证方法。该模型基于THUMS (Total Human Model for Safety,虚拟人体模型)确定缩放比例,通过人体解剖学参数等数据,验证模型的几何参数以及不同力学工况下人体腰臀部模型的生物力学响应准确性,结果显示,模型几何参数与质量参数误差均在5%以内,满足使用要求,同时动态工况下臀部生物力学响应结果和准静态、静态工况下腰椎生物力学响应结果均在误差允许范围内,从而验证模型的有效性。所构建的模型及验证方法是汽车座椅舒适性研究的有效工具。

关键词：中国50百分位人体体征有限元模型生物力学腰椎动态工况座椅舒适性

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Effect of vibration on interfacial microstructure and mechanical properties of Mg/Al bimetal prepared by a novel compound casting

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Journal of Magnesium and Alloys 2022年第8期10卷 2296-2309页

作者： Feng guan Wenming Jiang guangyu Li junwen Zhu Junlong Wang Guoliang Jie Zitian Fan State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China

In this work,a vibration was applied in the preparation of the Mg/Al bimetal by a novel compound casting in order to improve the mechanical properties of the Mg/Al bimetal,and the effect of the vibration on the interfacial microstructure and mechanical properties of the Mg/Al bimetal was investigated.The results indicated that the vibration had a significant effect on the interfacial microstructure and mechanical properties of the Mg/Al bimetal,but it did not change the phase compositions of the interface,which was composed of layerⅠ(Al3Mg2+Mg2Si),layerⅡ(Al_(12)Mg_(17)+Mg_(2)Si)and layerⅢ(Al_(12)Mg_(17)/δ-Mg).Without vibration,the Mg_(2)Si phase with a needle-like morphology mainly aggregated in the layerⅡof the interface.After the application of the vibration,the SEM and EBSD analysis results showed that the Mg_(2)Si and Al3Mg2phases in the interface were obviously refined,and the distribution of the Mg_(2)Si became more uniform,due to the strong forced convection of the molten metal resulting from the vibration.The TEM analysis indicated that the interface between the A_(l3)Mg_(2) and Mg_(2)Si phases was non-coherent,suggesting the Mg_(2)Si particles cannot act as a heterogeneous nucleation base during the solidification process of the interface.Compared to the Mg/Al bimetal without vibration,the shear strength of the Mg/Al bimetal with vibration increased by about 50%from 31.7 MPa on average to 47.5 MPa,and the hardness of the layer I of the interface increased,and the hardness of the layerⅢdecreased.The fracture surface transformed from a flat fracture morphology without vibration to an irregular zigzag fracture morphology.

关键词： Mg/Al bimetal Vibration Compound casting Interfacial microstructure Bonding properties

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