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Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1871-1886页

作者： Yongbao Wu Jing Tang Zhiguo Wen Bo Zhang junting cao Lulu Zhao Zhanbao Guo Ming Xie Zhengkui Zhou Shuisheng Hou State Key Laboratory of Animal Nutrition Key Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China

Background:Although methionine(Met),the first-limiting dietary amino acid,has crucial roles in growth and regulation of lipid metabolism in ducks,mechanisms underlying are not well understood.Therefore,the objective was to use dietary Met deficiency to investigate the involvement of Met in lipid metabolism and fat accumulation of Pekin ducks.Methods:A total of 150 male Pekin ducks(15-d-old,558.5±4.4 g)were allocated into 5 groups(6 replicates with 5 birds each)and fed corn and soybean meal-based diets containing 0.28%,0.35%,0.43%,0.50%,and 0.58%Met,respectively,for 4 weeks.Met-deficient(Met-D,0.28%Met)and Met-adequate(Met-A,0.43%Met)groups were selected for subsequent molecular studies.Serum,liver,and abdominal fat samples were collected to assess the genes and proteins involved in lipid metabolism of Pekin ducks and hepatocytes were cultured in vivo for verification.Results:Dietary Met deficiency caused growth depression and excess fat deposition that were ameliorated by feeding diets with adequate Met.Serum triglyceride and non-esterified fatty acid concentrations increased(P<0.05),whereas serum concentrations of total cholesterol,low density lipoprotein cholesterol,total protein,and albumin decreased(P<0.05)in Met-D ducks compared to those in Met-A ducks.Based on hepatic proteomics analyses,dietary Met deficiency suppressed expression of key proteins related to fatty acid transport,fatty acid oxidation,tricarboxylic acid cycle,glycolysis/gluconeogenesis,ketogenesis,and electron transport chain;selected key proteins had similar expression patterns verified by qRT-PCR and Western blotting,which indicated these processes were likely impaired.In vitro verification with hepatocyte models confirmed albumin expression was diminished by Met deficiency.Additionally,in abdominal fat,dietary Met deficiency increased adipocyte diameter and area(P<0.05),and down-regulated(P<0.05)of lipolytic genes and proteins,suggesting Met deficiency may suppress lipolysis in adipocyte.Conclusion:Taken together,these data demonstrated th

关键词： Fat deposition Hepatic catabolism Methionine deficiency Pekin duck Proteomics

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Sulfur-substitution-enhanced crystallization and crystal structure of poly(trimethylene monothiocarbonate)

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Chinese Chemical Letters 2022年第2期33卷 1021-1024页

作者： Xiaohan cao Hongliang Wang Jialiang Yang Ruiyang Wang Xin Hong Xinghong Zhang junting Xu Hai Wang MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Center of Chemistry for Frontier Technologies Zhejiang UniversityHangzhou 310027China Department of Chemistry Zhejiang UniversityHangzhou 310027China Department of Polymer Science and Engineering College of Chemical EngineeringDalian University of TechnologyDalian 116024China

In this paper,the crystallization behavior of a novel poly(monothiocarbonate),poly(trimethylene monothiocarbonate)(PTMMTC),was investigated and compared with its polycarbonate analogue,poly(trimethylene carbonate)(PTMC).It is found that PTMMTC exhibits strong crystallizability,while un-stretched PTMC is amorphous.DSC and DMA results reveal that PTMMTC possesses higher glass transition temperature(T_(g))andβ-transition temperature(T_(β))than PTMC.Simulation based on density functional theory(DFT)shows that,the bond angle of C-S-C is evidently smaller than that of C-O-C,and thus a larger dipole moment.This leads to the stronger intermolecular interaction and more rigid chain confor-mation in PTMMTC,which is the origin of sulfur-substitution enhanced crystallization.The crystal struc-ture of PTMMTC was preliminarily determined for the first time.PTMMTC has an orthorhombic crystal structure with a planar zig-zag chain conformation.The parameters of unit cell are a=10.74 A,b=4.79 A,and c(fiber axis)=7.74 A.

关键词： Poly(monothiocarbonate) Polycarbonate Crystallization behavior Crystal structure Sulfur substitution enhanced crystallization

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Scalable and general synthesis of spinel manganese- based cathodes with hierarchical yolk-shell structure and superior lithium storage properties

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Nano Research 2018年第1期11卷 246-253页

作者： Yu Wu junting Zhang Chuanbao cao Research Center of Materials Science Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and GreenApplications Beijing Institute of Technology Beijing 10008 I China

Hierarchical yolk-shell structured cathodes with controllable composition are potentially attractive materials for the fabrication of lithium-ion batteries, but they are difficult to synthesize. In this work, we present a simple, scalable, and general morphology-inheritance strategy to synthesize spinel manganese cathodes with a hierarchical yolk-shell structure. Starting from uniform Mn carbonate spheres prepared by an ultrafast and scalable microwave-assisted method, we show that the subsequent sintering results in the formation of Mn203 precursors with a yolk-shell structure, which can be effectively transferred to spinel manganese cathodes via simple impregnation and solid-state reaction. Owing to the simple and scalable nature of the present strategy, materials prepared through this approach have great potential as cathodes of lithium-ion batteries, where they can lead to high specific capacity, outstanding cyclability, and superior rate capability. In particular, both LiMn204 and LiNi05Mn1504 with hierarchical yolk-shell structure achieved nearly theoretical capacity, without any apparent decay after 100 cycles at I C. Moreover, 80% of the initial discharge capacities of both samples can be maintained for up to 500 cycles at a high rate of 10 C.

关键词： yolk-shell structures,microwave-assistedmethod,scalable synthesis,spinel manganese cathodes,lithium-ion batteries

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Exploration about superior anti-counterfeiting ability of Sm^(3+) doped KSr_(2)Nb_(5)O_(15) photochromic ceramics:Origin and atomic-scale mechanism

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Journal of Materiomics 2022年第1期8卷 38-46页

作者： Shuyao cao Jiatong Zhu Qian Chen junting Liu Changying Wu Leilei Li Jie Xu Haixue Yan Feng Gao State Key Laboratory of Solidification Processing MIIT Key Laboratory of Radiation Detection Materials and DevicesUSI Institute of Intelligence Materials and StructureNPU-QMUL Joint Research Institute of Advanced Materials and StructureSchool of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi'an710072China School of Electronics and Information Northwestern Polytechnical University710072Xi'anShaanxiChina Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen518057China School of Engineering and Materials Science Queen Mary University of LondonMile End RoadLondonE14NSUK

Reversible luminescence modulation behavior upon the photochromic effect endows the photochromic ceramics with 3reat potential in anti-counterfeiting and data storage applications.Here,Sm^(3+)-doped KSr_(2)Nb_(5)O_(15) photochromic ceramics exhibit superior anti-counterfeiting ability:good covertness and considerable modulation ratio of luminescent emission intensity after photochromic reaction.The results show that the photochromism originated from oxygen and cation vacancies,which were directly identified by electron paramagnetic resonance and positron annihilation lifetime spectra.Unexpectedly,oxygen vacancies work more effectively than cation vacancies during photochromic reactions.Moreover,the extraordinary anti-counterfeiting ability was attributed to the high energy transfer rate,which was particularly caused by the short mean distance below 1 nm between the Sm^(3+) and vacancies.The work here has provided atomic-scale structural evidence and made a progress in understanding the photochromic origins and mechanism in color-center theory.

关键词： Tetragonal tungsten bronze structure Photochromism Luminescence modulation Atomic-scale analysis

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喷施槲皮素对板栗生长及坚果品质的影响

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中国果树 2024年第2期 75-81,95页

作者：张腾飞靳常敏于克妍曾扬鹃靳俊婷郭春磊于立洋曹飞河北科技师范学院园艺科技学院秦皇岛066004 板栗产业技术教育部工程研究中心河北省特色园艺种质挖掘与创新利用重点实验室河北省板栗产业协同创新中心

为了探明喷施槲皮素对板栗生长及坚果品质的影响,为槲皮素在板栗生产中的应用提供科学依据。以6年生燕山早丰板栗嫁接树为试材,以不同浓度(0、150、300、600、1 200μmol/L)槲皮素进行叶面喷施,单株喷施500 mL,探讨对板栗叶片抗性指标... 详细信息

为了探明喷施槲皮素对板栗生长及坚果品质的影响,为槲皮素在板栗生产中的应用提供科学依据。以6年生燕山早丰板栗嫁接树为试材,以不同浓度(0、150、300、600、1 200μmol/L)槲皮素进行叶面喷施,单株喷施500 mL,探讨对板栗叶片抗性指标、结果枝生长、产量及品质的影响。结果表明:喷施1 200μmol/L槲皮素可显著提高板栗叶片APX活性、CAT活性、可溶性糖含量、蛋白质含量,分别较对照提高了141.2%、75.3%、40.0%、93.6%;喷施槲皮素可降低板栗空苞率,但对板栗枝条生长和产量影响不显著;喷施槲皮素主要提高了坚果可溶性糖含量与淀粉含量,喷施1 200μmol/L槲皮素可溶性糖含量最高,较对照提升30.2%,喷施600μmol/L槲皮素淀粉含量最高,较对照提升20.7%。通过主成分分析及隶属函数综合评价得出,喷施1 200μmol/L槲皮素为最优处理。

关键词：槲皮素喷施板栗叶片坚果品质

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Kilometers Long Graphene-Coated Optical Fibers for Fast Thermal Sensing

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Research 2021年第1期2021卷 286-294页

作者： Yiyong Guo Bing Han junting Du Shanshan cao Hua Gao Ning An Yiwei Li Shujie An Zengling Ran Yue Lin Wencai Ren Yunjiang Rao Baicheng Yao Key Laboratory of Optical Fiber Sensing and Communications(Education Ministry of China) University of Electronic Science and Technology of ChinaChengdu 611731China Research Centre of Optical Fiber Sensing Zhejiang LaboratoryHangzhou 310000China Optical Fiber Co. Ltd.ZTT GroupNantong 226009China Carbonene Technology Co. LtdDeyang 618000China Optical Science and Technology Ltd. China National Petroleum CorporationChengdu 610041China Cavendish Laboratory University of CambridgeCB30HEUK Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China

The combination of optical fiber with graphene has greatly expanded the application regimes of fiber optics,from dynamic optical control and ultrafast pulse generation to high precision sensing.However,limited by fabrication,previous graphene-fiber samples are typically limited in the micrometer to centimeter scale,which cannot take the inherent advantage of optical fibers—longdistance optical transmission.Here,we demonstrate kilometers long graphene-coated optical fiber(GCF)based on industrial graphene nanosheets and coating technique.The GCF shows unusually high thermal diffusivity of 24.99 mm^(2) s^(-1) in the axial direction,measured by a thermal imager directly.This enables rapid thermooptical response both in optical fiber Bragg grating sensors at one point(18-fold faster than conventional fiber)and in long-distance distributed fiber sensing systems based on backward Rayleigh scattering in optical fiber(15-fold faster than conventional fiber).This work realizes the industrial-level graphene-fiber production and provides a novel platform for two-dimensional material-based optical fiber sensing applications.

关键词： fiber scattering faster

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Somatic GJA4 mutation in intracranial extra-axial cavernous hemangiomas

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Stroke & Vascular Neurology 2023年第6期8卷 453-462,I0003-I0037页

作者： Ran Huo Yingxi Yang Hongyuan Xu Shaozhi Zhao Dong Song Jiancong Weng Ruochen Ma Yingfan Sun Jie Wang Yuming Jiao Junze Zhang Qiheng He Ruolei Wu Shuo Wang Ji-Zong Zhao junting Zhang Jiguang Wang Yong cao Department of Neurosurgery Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases BeijingChina Department of Chemical and Biological Engineering The Hong Kong University of Science and TechnologyHong KongChina Division of Life Science Center for Systems Biology and Human Health and State Key Laboratory of Molecular NeuroscienceThe Hong Kong University of Science and TechnologyHong KongChina Department of Neurosurgery China-Japan Friendship HospitalBeijingChina School of Medical Technology Beijing Institute of TechnologyBeijingChina Hong Kong Center for Neurodegenerative Diseases InnoHKHong Kong SARChina Beijing Neurosurgical Institute Capital Medical UniversityBeijingChina

Objective Extra-axial cavernous hemangiomas(ECHs)are sporadic and rare intracranial occupational lesions that usually occur within the cavernous sinus.The aetiology of ECHs remains unknown.Methods Whole-exome sequencing was performed on ECH lesions from 12 patients(discovery cohort)and droplet digital polymerase-chain reaction(ddPCR)was used to confirm the identified mutation in 46 additional cases(validation cohort).Laser capture microdissection(LCM)was carried out to capture and characterise subgroups of tissue cells.Mechanistic and functional investigations were carried out in human umbilical vein endothelial cells and a newly established mouse model.Results We detected somatic GJA4 mutation(c.121G>T,p.G41C)in 5/12 patients with ECH in the discovery cohort and confirmed the finding in the validation cohort(16/46).LCM followed by ddPCR revealed that the mutation was enriched in lesional endothelium.In vitro experiments in endothelial cells demonstrated that the GJA4 mutation activated SGK-1 signalling that in turn upregulated key genes involved in cell hyperproliferation and the loss of arterial specification.Compared with wild-type littermates,mice overexpressing the GJA4 mutation developed ECH-like pathological morphological characteristics(dilated venous lumen and elevated vascular density)in the retinal superficial vascular plexus at the postnatal 3 weeks,which were reversed by an SGK1 inhibitor,EMD638683.Conclusions We identified a somatic GJA4 mutation that presents in over one-third of ECH lesions and proposed that ECHs are vascular malformations due to GJA4-induced activation of the SGK1 signalling pathway in brain endothelial cells.

关键词： intracranial superficial hemangioma

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Microstructure and bidirectional dielectric tunability behaviour of Nd^(3+)-doped KSr_(2)Nb_(5)O_(15) lead-free ceramics

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Journal of Materiomics 2021年第5期7卷 976-987页

作者： Shuyao cao Jie Xu Li Jin Jia Zhao Zhida Ma Qian Chen junting Liu Feng Gao State Key Laboratory of Solidification Processing MIIT Key Laboratory of Radiation Detection Materials and DevicesUSI Institute of Intelligence Materials and StructureNPU-QMUL Joint Research Institute of Advanced Materials and StructureSchool of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an710072China Electronic Materials Research Laboratory Key Laboratory of the Ministry of Education&International Center for Dielectric ResearchSchool of Electronic Science and EngineeringFaculty of Electronic and Information EngineeringXi’an Jiaotong UniversityXi’an710049China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen518057China

The luminescence modulation behaviour under the in-situ electric field of rare-earth doped KSr_(2)Nb_(5)O_(15) ceramics opened a new door for the development of dielectric materials.Where the understanding the effect of rare-earth doping on the electric properties of host,especially at the similar doping concentration with luminescence researches(low concentrations)is very important for the exploration of mechanism of electric-luminescent coupling effect.In this work,Nd^(3+)-doped KSr_(2)Nb_(5)O_(15)(KSN-xNd)ceramics were synthesized,and the electric properties were investigated systematically.Our results suggest that the Nd^(3+) doping slightly increased the phase transition temperatures and improved the piezoelectric response of KSr_(2)Nb_(5)O_(15).Most importantly,a bidirectional dielectric tunability is revealed in KSr_(2)Nb_(5)O_(15).The dielectric permittivity can be adjusted by the DC electric bias,with tunability ranging from12.3% to 21.9%.The related mechanism and relationship between the bidirectional dielectric tunability and ferroelectricity are revealed by temperature-dependent dielectric and ferroelectric characterization.The researches of electric properties and bidirectional dielectric tunability of KSN-based ceramics paved the way to further exploration of electric-luminescent coupling mechanism.

关键词： Tungsten bronze type structure KSr_(2)Nb_(5)O_(15) Nd^(3+)doping Bidirectional dielectric tunability

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无筋超高性能混凝土板的受弯性能及承载力计算

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湖南大学学报（自然科学版） 2023年第5期50卷 29-42页

作者：苏捷陈俊廷方志陈佳醒曹清周腾湖南大学土木工程学院湖南长沙410082 绿色先进土木工程材料及应用技术湖南省重点实验室(湖南大学) 湖南长沙410082 风工程与桥梁工程湖南省重点实验室(湖南大学) 湖南长沙410082 广东省交通规划设计研究院集团股份有限公司广东广州510507

为明确无筋超高性能混凝土(Ultra High Performance Concrete,UHPC)单向板和周边支承方板的受弯性能,分别对其进行了跨中局部荷载作用下受弯性能的破坏性试验.基于本文及其他文献的试验结果,考虑钢纤维特征参数的影响,建立了UHPC材料的... 详细信息

为明确无筋超高性能混凝土(Ultra High Performance Concrete,UHPC)单向板和周边支承方板的受弯性能,分别对其进行了跨中局部荷载作用下受弯性能的破坏性试验.基于本文及其他文献的试验结果,考虑钢纤维特征参数的影响,建立了UHPC材料的受拉本构.通过数值分析,提出了无筋UHPC板截面受拉区等效均布应力折减系数k的计算公式.根据试验和分析结果,建立了无筋UHPC单向板和周边支承方板抗弯承载能力的简化计算方法.结果表明:无筋UHPC单向板和周边支承方板均发生由UHPC抗拉性能所控制的受拉破坏.由于UHPC内钢纤维的增强作用,无筋UHPC板的抗弯承载能力和极限变形分别较相应的开裂荷载和开裂变形明显提高且表现出一定的延性破坏特征,但UHPC的受拉塑性尚不足以保证周边支承方板中完全塑性铰线机构的形成,塑性铰线法的上限解不适于预测周边支承方板的极限荷载,而静力法的下限解却能给出精度较高且偏于安全的预测结果;试验结果验证了所提无筋UHPC单向板和周边支承方板极限承载能力简化计算方法的适用性.

关键词：超高性能混凝土单向板方板承载能力试验研究计算方法

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双极传输供体-受体(D-A)型铱(Ⅲ)配合物近红外发光材料的合成及其电致发光性能

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有机化学 2022年第6期42卷 1831-1838页

作者：曹丽琴杨小琴李茂秋刘琳于俊婷谭华湖南科技大学化学化工学院理论有机化学与功能分子教育部重点实验室湖南湘潭411201 常州大学材料科学与工程学院江苏省光电热能量转化材料与应用工程实验室江苏常州213164

将具有电子传输性能的二苯砜基团(PS)和空穴传输性能的咔唑(Cz)基团共价连接到吡啶甲酸(pic)上,合成了具有双极传输特性的辅助配体pic-PSCz,并构筑了一种双极传输的供体-受体(D-A)型铱(Ⅲ)配合物(t BuTPA-BTzIq)2Ir(pic-PSCz)(t Bu为叔... 详细信息

将具有电子传输性能的二苯砜基团(PS)和空穴传输性能的咔唑(Cz)基团共价连接到吡啶甲酸(pic)上,合成了具有双极传输特性的辅助配体pic-PSCz,并构筑了一种双极传输的供体-受体(D-A)型铱(Ⅲ)配合物(t BuTPA-BTzIq)2Ir(pic-PSCz)(t Bu为叔丁基,TPA为三苯胺,BTz为苯并三唑,Iq为异喹啉)近红外电致发光材料.通过紫外-可见吸收光谱和光致发光光谱研究发现,相比于配合物(Piq)2Ir(pic-PSCz),(t BuTPA-BTz-Iq)2Ir(pic-PSCz)由于分子内较强的D-A作用,其发射光谱红移约110 nm.与已报道的配合物(TPA-BTz-Iq)2Irpic相比,(t BuTPA-BTz-Iq)2Ir(pic-PSCz)分子内引入Cz和PS基团,可以有效提高其电致发光效率,其器件最大发射峰位于716 nm,最大外量子效率(EQE_(max))为0.87%,为(TPA-BTz-Iq)2Irpic器件效率的3倍(EQE_(max)为0.29%@712 nm).

关键词：双极传输供体-受体近红外铱(Ⅲ)配合物有机电致发光器件

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