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基于“R-S-A”三点位在危重症患者超声引导下鼻肠管置入技术的初步探索

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陆军军医大学学报 2024年第18期46卷 2145-2151页

作者：陈双梅刘若萱谭亮苏小洪刘美麟齐俊生曹英陆军军医大学第一附属医院重症医学科重庆400038

目的本研究旨在借助超声可视化特点,结合“R-S-A”三点位行危重症患者超声引导下鼻肠管置入,并评估此技术的精准度、有效性。方法对重庆市某三级甲等医院重症医学科2022年4月-2023年8月收治的全部危重症鼻肠管置管患者进行回顾性分析。... 详细信息

目的本研究旨在借助超声可视化特点,结合“R-S-A”三点位行危重症患者超声引导下鼻肠管置入,并评估此技术的精准度、有效性。方法对重庆市某三级甲等医院重症医学科2022年4月-2023年8月收治的全部危重症鼻肠管置管患者进行回顾性分析。根据置管体位分为对照组与观察组,对照组行超声引导下鼻肠管置入,观察组在超声引导的基础上以“R-S-A”三点站位(患者取右侧卧位,置管者站于患者右肩处,超声操作者站于右腹部操作方向)行超声引导下鼻肠管置入,对照组仅在超声引导下行鼻肠管置入,对体位无特殊要求。倾向性评分匹配后比较两组的置管时间、置管成功率、并发症发生情况。结果观察组平均置管时间(36.2±10.3)min短于对照组(42.3±8.3)min,置管成功率95.2%高于对照组66.7%,差异均具有统计学意义(P0.05,差异无统计学意义。结论基于“R-S-A”三点位行危重患者超声引导下鼻肠管置入技术精准度高、有效性强,能够缩短置管时间,提高置管成功率。

关键词：危重患者体位超声引导鼻肠管置入

Occurrence and infection risk of waterborne pathogens in Wanzhou watershed of the Three Gorges Reservoir, China

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Journal of Environmental Sciences 2013年第9期25卷 1913-1924页

作者： Guosheng Xiao Zhaodan Wang Ji'an Chen Zhiqun qiu Yanjie Li junsheng qi Wenyi Liu Weiqun Shu Department of Environmental Hygiene School of Military Preventive Medicine Third Military Medical University College of Life Science and Engineering Chongqing Three Gorges University

The Three Gorges Reservoir （TGR）, formed by China＇s Yangtze Three Gorges Project, is the largest lake in the world, but there is too little information available about fecal contamination and waterborne pathogen impacts on this aquatic ecosystem. During two successive 1-year study periods （July 2009 to July 2011）, the water quality in Wanzhou watershed of the TGR was tested with regard to the presence of fecal indicators and pathogens. According to Chinese and World Health Organization water quality standards, water quality in the mainstream was good but poor in backwater areas. Salmonella, Enterohemorrhagic Escherichia coli （EHEC）, Giardia and Cryptosporidium were detected in the watershed. Prevalence and concentrations of the pathogens in the mainstream were lower than those in backwater areas. The estimated risk of infection with Salmonella, EHEC, Cryptosporidium, and Giardia per exposure event ranged from 2.9 × 10 -7 to 1.68 × 10-5 , 7.04 × 10-10 to 2.36 × 10-7 , 5.39 × 10-6 to 1.25 × 10-4 and 0 to 1.2 × 10-3 , respectively, for occupational divers and recreational swimmers exposed to the waters. The estimated risk of infection at exposure to the 95% upper confidence limit concentrations of Salmonella, Cryptosporidium and Giardia may be up to 2.62 × 10-5 , 2.55 × 10-4 and 2.86 × 10-3 , respectively. This study provides useful information for the residents, health care workers and managers to improve the safety of surface water and reduce the risk of fecal contamination in the TGR.

关键词： the Three Gorges Reservoir microbial risk assessment fecal contamination waterborne pathogen fecal indicator

RAF22,ABI1 and OST1 form a dynamic interactive network that optimizes plant growth and responses to drought stress in Arabidopsis

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Molecular Plant 2022年第7期15卷 1192-1210页

作者： Zhihui Sun Zhenkai Feng Yanglin Ding Yuanpeng qi Shan Jiang Zhen Li Yu Wang junsheng qi Chunpeng Song Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China

Plants adapt to their ever-changing environment via positive and negative signals induced by environmental *** stress,for instance,induces accumulation of the plant hormone abscisic acid(ABA),triggering ABA signal ***,the molecular mechanisms for switching between plant growth promotion and stress response remain poorly *** we report that RAF(rapidly accelerated fibrosarcoma)-LIKE MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 22(RAF22)in Arabidopsis tha/iana physically interacts with ABA INSENSITIVE 1(ABl1)and phosphorylates ABl1 at Ser416 residue to enhance its phosphatase ***,ABl1 can also enhance the activity of RAF22 through dephosphorylation,reciprocally inhibiting ABA signaling and promoting the maintenance of plant growth under normal *** drought stress,however,the ASA-activated OPEN STOMATA1(OST1)phosphorylates the Ser81 residue of RAF22 and inhibits its kinase activity,restraining its enhancement of ABl1 *** together,our study reveals that RAF22,ABl1,and OST1 form a dynamic regulatory network that plays crucial roles in optimizing plant growth and environmental adaptation under drought stress.

关键词： ABA signaling RAF22 ABl1 OST1 phosphorylation

Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack

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Journal of Integrative Plant Biology 2018年第9期60卷 805-826页

作者： junsheng qi Chun-Peng Song Baoshan Wang Jianmin Zhou Jaakko Kangasjarvi Jian-Kang Zhu Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Henan ProvinceInstitute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant Stress Research College of Life ScienceShandong Normal UniversityJi'nan 250000China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Division of Plant Biology Viikki Plant Science CentreDepartment of BiosciencesUniversity of Helsinki00014 HelsinkiFinland Shanghai Center for Plant Stress Biology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907USA

Stomata, the pores formed by a pair of guard cells, are the main gateways for water transpiration and photosynthetic CO2 exchange, as well as pathogen invasion in land plants. Guard cell movement is regulated by a combination of environmental factors, including water status, light, CO2 levels and pathogen attack, as well as endogenous signals, such as abscisic acid and apoplastic reactive oxygen species （ROS）. Under abiotic and biotic stress conditions, extracellular ROS are mainly produced by plasma membrane-localized NADPH oxidases, whereas intracellular ROS are produced in multiple organelles. These ROS form a sophisticated cellular signaling network, with the accumulation of apoplastic ROS an early hallmark of stomatal movement. Here, we review recent progress in understanding the molecular mechanisms of the ROS signaling network, primarily during drought stress and pathogen attack. We summarize the roles of apoplastic ROS in regulating stomatal movement, ABA and CO2 signaling, and immunity responses. Finally, we discuss ROS accumulation and communication between organelles and cells. This information provides a conceptual framework for understanding how ROS signaling is integrated with various signaling pathways during plant responses to abiotic and biotic stress stimuli.

关键词： ROS Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack ABA

GbAt11 gene cloned from Gossypium barbadense mediates resistance to Verticillium wilt in Gossypium hirsutum

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Journal of Cotton Research 2020年第2期3卷 69-78页

作者： qiU Tingting WANG Yanjun JIANG Juan ZHAO Jia WANG Yanqing qi junsheng State Key Laboratory of Plant Physiology and Biochemistry Department of Plant ScienceCollege of Biology ScienceChina Agricultural UniversityBeijing 100193China

Background:Gossypium hirsutum is highly susceptible to Verticillium wilt,and once infected Verticillium wilt,its yield is greatly *** *** is highly resistant to Verticillium *** is possible that transferring some disease-resistant genes from *** to *** may contribute to *** resistance to Verticillium ***:Here,we described a new gene in *** encoding AXMN Toxin Induced Protein-11,Gb At11,which is specifically induced by Verticillium dahliae in *** and enhances Verticillium wilt resistance in *** in *** not only significantly improves resistance to Verticillium wilt,but also increases the boll number per *** analysis and real-time polymerase chain reaction showed that Gb At11 overexpression can simultaneously activate FLS2,BAK1 and other genes,which are involved in ETI and PTI pathways in ***:These data suggest that Gb At11 plays a very important role in resistance to Verticillium wilt in *** it is significant for improving resistance to Verticillium wilt and breeding high-yield cotton cultivars.

关键词： Gossypium barbadense GbAt11 Verticillium wilt resistance

BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis

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Journal of Integrative Plant Biology 2022年第6期64卷 1264-1280页

作者： Jinping Deng Lingyao Kong Yinhua Zhu Dan Pei Xuexue Chen Yu Wang junsheng qi Chunpeng Song Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Qingdao UniversityQingdao 266071China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001HenanChina Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071002China

The mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid(ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1(BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1(OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1-1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1-2 abi2-2 hab1-1 loss-of-function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2 Cs,and Sn RK2 s, during seed germination and primary root growth. Although the upstream brassinosteroid(BR) signaling components BAK1 and BR INSENSITIVE 1(BRI1) positively regulate ABAinduced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID-SIGNALING KINASEs(BSKs)and BRASSINOSTEROID-INSENSITIVE 2(BIN2), did not affect ABA-mediated stomatal movement. Thus,our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA-induced stomatal closure, thus optimizing the plant growth under drought stress.

关键词： abscisic acid BAK1 primary root growth stomatai closure

Arabidopsis U-box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor-like protein kinases LRR1 and KIN7

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Journal of Integrative Plant Biology 2021年第3期63卷 494-509页

作者： Xuexue Chen Tingting Wang Amin Ur Rehman Yu Wang junsheng qi Zhen Li Chunpeng Song Baoshan Wang Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant Stress Research College of Life ScienceShandong Normal UniversityJi'nan 250000China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China

Both plant receptor-like protein kinases(RLKs)and ubiquitin-mediated proteolysis play crucial roles in plant responses to drought ***,the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly *** this study,we showed that Arabidopsis PLANT U-BOX PROTEIN 11(PUB11),an E3 ubiquitin ligase,negatively regulates abscisic acid(ABA)-mediated drought ***11 interacts with and ubiquitinates two receptor-like protein kinases,LEUCINE RICH REPEAT PROTEIN 1(LRR1)and KINASE 7(KIN7),and mediates their degradation during plant responses to drought stress in vitro and in ***11 mutants were more tolerant,whereas Irr1 and kin7 mutants were more sensitive,to drought stress than the wild *** analyses show that the pub11 Irr1 kin7 triple mutant exhibited similar drought sensitivity as the Irr1 kin7 double mutant,placing PUB11 upstream of the two *** acid and drought treatment promoted the accumulation of PUB11,which likely accelerates LRR1 and KIN7 ***,our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.

关键词： abscisic acid drought response E3 ligase receptor-like kinase ubiquitination

2种免疫诱抗剂和常规药剂在小麦上的应用比较

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浙江农业科学 2024年第3期65卷 672-675页

作者：张付强张红杰张国于居龙王俊文齐俊生河北北方学院河北张家口075000 中捷四方生物科技股份有限公司陕西杨凌712100 江苏丘陵地区镇江农业科学研究所江苏镇江212499 中国农业大学生物学院北京100193

为了验证免疫诱抗剂对小麦具有促生、防病、增产和提质的效果,本研究通过对比维大力、氨基寡糖素、常规化学药剂和空白对照在小麦拌种及叶面喷施应用的效果,研究其对小麦秧苗素质、纹枯病防治效果、赤霉病防治效果、产量及呕吐毒素的影... 详细信息

为了验证免疫诱抗剂对小麦具有促生、防病、增产和提质的效果,本研究通过对比维大力、氨基寡糖素、常规化学药剂和空白对照在小麦拌种及叶面喷施应用的效果,研究其对小麦秧苗素质、纹枯病防治效果、赤霉病防治效果、产量及呕吐毒素的影响。结果显示,利用维大力进行小麦拌种处理能显著增加小麦根长,促进次生根产生;在小麦拔节期喷施免疫诱抗剂对纹枯病防治效果达83.33%,显著优于常规药剂;在小麦抽穗扬花期喷施免疫诱抗剂对赤霉病防治效果达96.45%~98.05%,显著降低了呕吐毒素产生;利用免疫诱抗剂在拔节期和扬花期与常规药剂配合使用,可显著提高小麦籽粒实粒数,较单用常规药剂增产12.1%~13.7%。免疫诱抗剂在小麦应用上具有较高价值。

关键词：植物免疫诱抗剂维大力秧苗素质抗病产量呕吐毒素

Study on Rice Water Requirement of Liangping County under Climate Change Background

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Meteorological and Environmental Research 2014年第9期5卷 57-59页

作者： Gao Fei qi junsheng Wang qinqin Zhang Ting Mou Xinli Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservoir ( Chongqing Three Gorges University )

Under global climate change background,using daily meteorological data at Liangping ground meteorological station during 1961- 2012,we calculated crop water requirement and net irrigation water requirement during rice growth period in Liangping County,and analyzed its climate tendency rate. Results showed that climate tendency rate of crop water requirement during growth period of rice was only- 0. 007 mm /10 a; climate tendency rate of rainfall was- 0. 06 mm /10 a,but interannual change was relatively larger; climate tendency rate of net irrigation water requirement was 0. 011 mm /10 a. In the years when drought occurred,such as 2006 and 2011,both rice water requirement and net irrigation water requirement in Liangping were greatly higher than means over the years. Therefore,we should focus on drought pre-warning and risk management improving drought disaster prevention in Liangping in the future.

关键词： Liangping County Rice Crop water requirement Net irrigation water requirement Climate change China