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牛下丘脑lncRNA SNHG3的筛选及其调控CART表达的功能鉴定

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生物工程学报 2024年第7期40卷 2162-2177页

作者：郝琴琴闫俊蓉任静成俊丽朱芷葳李鹏飞山西农业大学生命科学学院山西太谷030801

本研究旨在筛选调控牛下丘脑可卡因-苯丙胺调节转录肽(cocaine and amphetamine regulated transcript peptide,CART)表达的长链非编码RNA (long non-coding RNA,lncRNA)小核仁RNA宿主基因3 (small nucleolar RNA host gene 3,SNHG3),明确lncRNA SNHG3调控牛下丘脑CART表达的作用机制。利用Star Base v2.0、NCBI、DIANA tools数据库预测分别与极显著抑制CART表达的miR-381、miR-491存在靶向关系的lncRNAs,分析其结合位点;选取3头健康成年西门塔尔母牛,提取其下丘脑组织总RNA,通过半定量RT-PCR技术鉴定所筛选的lncRNAs内源表达情况;双荧光素酶报告基因技术检测miR-381/491与lncRNAs间的靶向结合关系;构建lncRNAs、CART过表达载体及miR-381/491 mimics,分别转染至293T细胞,分析lncRNAs对CART基因表达的调控作用机制;利用动物活体实验对在细胞水平调控作用效果最强的lncRNA进行功能分析。lncRNA TUG1、SNHG3与miR-381存在结合位点,lncRNA H19、SNHG12、DANCR与miR-491存在结合位点,且lncRNA TUG1、SNHG3、H19、SNHG12、DANCR均在牛下丘脑中有表达;双荧光素酶检测结果显示,miR-381显著抑制TUG1-WT重组荧光质粒的相对荧光活性(P<0.05),极显著抑制SNHG3-WT重组荧光质粒的相对荧光活性(P<0.01);miR-491显著抑制DANCR-WT和H19-WT的荧光素酶活性表达(P<0.05),极显著抑制SNHG12-WT荧光素酶活性表达(P<0.01);在细胞水平,lncRNA SNHG3通过特异性结合miR-381极显著提高CART表达(P<0.001),lncRNA SNHG12通过特异性结合miR-491极显著提高CART表达(P<0.01),其中,lncRNA SNHG3调控CART表达效果最强;动物实验结果表明,lncRNA SNHG3通过特异性结合miR-381显著提高CART m RNA和蛋白表达。本研究证实了lncRNA SNHG3作为miR-381的竞争性内源RNA (competing endogenous RNAs,ce RNA),在转录和转录后水平均显著上调CART表达,为进一步研究牛下丘脑CART的分子网络调控机制奠定了基础。

关键词：牛卵泡发育下丘脑可卡因-苯丙胺调节转录肽(CART) 长链非编码RNA(lncRNAs) miR-381/491

侵袭性肺炎克雷伯菌肝脓肿综合征临床及影像学特征

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中国介入影像与治疗学 2024年第2期21卷 84-88页

作者：吴山杨美霞闫俊荣陈吉虎亢志琴山西白求恩医院放射科山西太原030032 山西白求恩医院急诊科山西太原030032

目的观察侵袭性肺炎克雷伯菌肝脓肿综合征(IKPLAS)临床及影像学特征。方法回顾性纳入68例肺炎克雷伯菌肝脓肿(KPLA)患者,根据是否发生肝外侵袭性感染分为IKPLAS组(n=25)及非IKPLAS组(n=43),比较组间临床资料及CT和/或MRI表现。结果IKP... 详细信息

目的观察侵袭性肺炎克雷伯菌肝脓肿综合征(IKPLAS)临床及影像学特征。方法回顾性纳入68例肺炎克雷伯菌肝脓肿(KPLA)患者,根据是否发生肝外侵袭性感染分为IKPLAS组(n=25)及非IKPLAS组(n=43),比较组间临床资料及CT和/或MRI表现。结果IKPLAS组患者年龄低于而糖化血红蛋白、D-二聚体均高于非IKPLAS组(P均<0.05)。IKPLAS组18例、非IKPLAS组6例可见肝静脉血栓性静脉炎,IKPLAS组10例、非IKPLAS组28例可见动脉期脓肿周围异常高灌注,组间差异均有统计学意义(P均<0.05)。IKPLAS组肝外感染主要累及肺(19/25,76.00%)。结论 IKPLAS患者年龄低于而糖化血红蛋白、D-二聚体高于其他KPLA;较常见肝静脉血栓性静脉炎及肝外感染主要累及肺为IKPLAS影像学特征。

关键词：肝脓肿肺炎克雷伯菌体层摄影术 X线计算机磁共振成像

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20例戈谢病遗传学特点和临床分析

中华血液学杂志 2024年第1期45卷 82-85页

作者：张恬波文晓玲张夏林闫俊荣郝国平杨林花张睿娟山西医科大学第三医院(山西白求恩医院、山西医学科学院、同济山西医院) 太原030032 宜宾市第一人民医院血液科宜宾644600 华中科技大学同济医学院附属同济医院武汉430030 山西省儿童医院血液科太原030013 山西医科大学第二医院血液科太原030001

戈谢病(Gaucher disease,GD)是一种常染色体隐性溶酶体贮积症,具有高度异质性。本研究通过回顾性分析山西白求恩医院20例GD患者的临床表现、实验室检查、酶学及基因结果,进一步了解GD患者临床表型与基因型相关性。20例GD患者中,Ⅰ型GD16... 详细信息

戈谢病(Gaucher disease,GD)是一种常染色体隐性溶酶体贮积症,具有高度异质性。本研究通过回顾性分析山西白求恩医院20例GD患者的临床表现、实验室检查、酶学及基因结果,进一步了解GD患者临床表型与基因型相关性。20例GD患者中,Ⅰ型GD16例,中位确诊年龄24岁;Ⅲ型GD 4例,中位确诊年龄19岁。所有患者均有脾大和血小板减少;16例患者有骨骼影像学改变,5例合并骨痛症状。20例患者共检出15种基因突变,多为错义突变,以L483P(35.7%)突变多见,其次为V414L、L303I、F252I,突变位点多见于7号外显子。其中,S310G突变为本课题组首次报道,K196R突变为中国人群首次报道,发现N227S突变可能与神经病变相关。GD患者临床表型与基因型之间仍存在不确定性。

关键词：常染色体隐性戈谢病临床表型溶酶体贮积症神经病变脾大影像学改变基因突变

EFFICIENT DESIGN OF ANTI-JAMMING RATELESS CODING IN COGNITIVE OFDM

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Journal of Electronics(China) 2010年第6期27卷 781-789页

作者： Xu Xiaorong Zhang Jianwu Zheng Baoyu yan junrong College of Telecommunication Engineering Hangzhou Dianzi University Hangzhou 310018 China Institute of Signal Processing and Transmission Nanjing University of Posts and Telecommunications Nanjing 210003 China

Efficient anti-jamming rateless coding based on cognitive Orthogonal Frequency Division Multiplexing (OFDM) modulation in Cognitive Radio Network (CRN) is mainly discussed. Rateless coding with small redundancy and low complexity is presented, and the optimal design methods of building rateless codes are also proposed. In CRN, anti-jamming rateless coding could recover the lost packets in parallel channels of cognitive OFDM, thus it protects Secondary Users (SUs) from the in-terference by Primary Users (PUs) efficiently. Frame Error Rate (FER) and throughput performance of SU employing anti-jamming rateless coding are analyzed in detail. Performance comparison between rateless coding and piecewise coding are also presented. It is shown that, anti-jamming rateless coding provides low FER and Word Error Rate (WER) performance with uniform sub-channel selection. Meanwhile, it is also verified that, in higher jamming rate and longer code redundancy scenario, rateless coding method could achieve better FER and throughput performance than another anti-jamming coding schemes.

关键词： Anti-jamming rateless coding Cognitive Orthogonal Frequency Division Multiplexing (OFDM) Frame Error Rate (FER) Word Error Rate (WER) Throughput performance

HYBRID INTERNET TRAFFIC CLASSIFICATION TECHNIQUE

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Journal of Electronics(China) 2009年第1期26卷 101-112页

作者： Li Jun Zhang Shunyi Lu yanqing yan junrong Nanjing University of Posts and Telecommunications Nanjing 210003 China Zhejiang Wanli University Ningbo 315100 China

Accurate and real-time classification of network traffic is significant to network operation and management such as QoS differentiation, traffic shaping and security surveillance. However, with many newly emerged P2P applications using dynamic port numbers, masquerading techniques, and payload encryption to avoid detection, traditional classification approaches turn to be ineffective. In this paper, we present a layered hybrid system to classify current Internet traffic, motivated by variety of network activities and their requirements of traffic classification. The proposed method could achieve fast and accurate traffic classification with low overheads and robustness to accommodate both known and unknown/encrypted applications. Furthermore, it is feasible to be used in the context of real-time traffic classification. Our experimental results show the distinct advantages of the proposed classifi- cation system, compared with the one-step Machine Learning (ML) approach.

关键词： Traffic classification Machine Learning (ML) Real-time identification

A novel acquired EGFR-SEPT14 fusion confers differential drug resistance to EGFR inhibitors in lung adenocarcinoma

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Genes & Diseases 2023年第6期10卷 2241-2244页

作者： Weidong Wang Wang Lv Hui Wang yang Xu junrong yan Han-Ming Shen Liqun Shan Jian Hu Department of Thoracic Surgery The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310003China Department of Thoracic Surgery The First People's Hospital of WenlingWenlingZhejiang 317599China Medical Department Nanjing Geneseeq Technology IncNanjingJiangsu 210000China Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore 119077Singapore Faculty of Health Sciences University of MacaoMacao SAR 519000China Department of Thoracic Surgery Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdong 510060China

Around 10%-30%of non-small cell lung cancer(NSCLC)patients harbored epidermal growth factor receptor(EGFR)mutations,with L858R and exon-19 deletions(19-Del)accounting for 90%of *** tyrosine kinase inhibitors(TKls)showed significant efficacy against common EGFR ***,the therapeutic relevance of uncommon EGFR mutations remained insufficiently *** fusions are extremely rare(0.05%-0.13%)in NSCLC,and Konduri group reported only 5 EGFR fusions from 10,097 patients.i2Additional EGFR'fusions were reported in NSCLC,3,4 all of which were oncogenic drivers and sensitive to EGFR ***,we reported an NSCLC patient with leptomeningeal metastasis(LM)who acquired a novel EGFR-SEPT14 fusion during TKI resistance and showed promising responses to certain EGFR TKls and intrathecal pemetrexed(IP).

关键词： lung acquired adenocarcinoma

Simplifying genetic classifiers by six mutated genes in diffuse large B-cell lymphoma

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Genes & Diseases 2023年第1期10卷 37-40页

作者： Jinghan Wang Fang Yu Wenwen Wei Jiansong Huang yang Shao junrong yan Liping Mao Wenjuan Yu Wanzhuo Xie Jie Jin Department of Hematology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang 310003China Key Laboratory of Hematologic Malignancies Diagnosis and TreatmentHangzhouZhejiang 310003China Zhejiang Provincial Clinical Research Center for Hematologic Diseases HangzhouZhejiang 310003China Cancer Center Zhejiang UniversityHangzhouZhejiang 310003China Department of Pathology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang 310003China Nanjing Geneseeq Technology Inc. NanjingJiangsu 210008China

Although diffuse large B-cell lymphoma(DLBCL)is considered as a curable disease after standard immunochemotherapy,approximately30%of patients would succumb to short *** date,clinical presentations and gene expressions... 详细信息

关键词： lymphoma chemotherapy patients

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山西平定方言“给给”的使用条件

语文研究 2006年第3期 64-64,F0003页

作者：延俊荣天津师范大学外国语学院天津300074

山西平定方言属于晋语阳泉片,其“给予”表达既有用“给”的情况,也有用“给给”的情况。本文将以“给”的分布为参照,描写平定方言“给给”的使用条件。

关键词：平定方言 “给给” 使用条件

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语义兼容对“V”“给”并置的制约

语文研究 2007年第4期 29-33页

作者：延俊荣天津师范大学外国语学院天津300387

作为构式,"NP1+V给+NP2+NP3"表达一个给予事件,但进入该构式的"V给"在句法表现上存在着差异。文章论证了典型"V"的语义属性包括[+方向性][+终点性][+途径性]。在此基础上标注了不同动词小类的语义属性。得出结论:"V"和"给"的[+方向性]... 详细信息

作为构式,"NP1+V给+NP2+NP3"表达一个给予事件,但进入该构式的"V给"在句法表现上存在着差异。文章论证了典型"V"的语义属性包括[+方向性][+终点性][+途径性]。在此基础上标注了不同动词小类的语义属性。得出结论:"V"和"给"的[+方向性]和[+终点性]是否相容制约着二者的并置与否。具体表现为:只要"V"与"给"[+方向性]不悖,二者即可并置。当"V"的[+终点性]是[+潜在的]或[-终点性]时,则"V"必须与"给"并置或共现才能表示"给予"。

关键词：语义兼容并置方向性终点性途径性