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An endophytic fungus interacts with the defensinlike protein OsCAL1 to regulate cadmium allocation in rice

Molecular Plant 2024年第2期17卷 312-324页

作者： Tianyu Gu Ziai Qi Yating wang Siying Chen Jing Yan Huapeng Qiu Yanxuan Yu Zjun Fang junmin wang Jiming Gong National Key Laboratory of Plant Molecular Genetics Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesChinese AcademyofSciencesShanghai200032China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China University of the Chinese Academy of Sciences Beijing 100049China Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization College of Life ScienceHunan University of Science and TechnologyXiangtan411201China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China Institute of Crops and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021China

Defensin-like proteins are conserved in multicellular organisms and contribute to innate immune re-sponses against fungal pathogens.In rice,defensins play a novel role in regulating cadmium(Cd)efflux from the cytosol.However,whether the antifungal activity of defensins correlates with Cd-efflux function remains unknown.In this study,we isolated an endophytic Fusarium,designed Fo1o,by a comparative mi-crobiome analysis of rice plants grown in a paddy contaminated with Cd.Fo10 is tolerant to high levels of Cd,but is sensitive to the defensin-like protein OsCAL1,which mediates Cd efflux to the apoplast.We found that Fo10 symbiosis in rice is regulated by OsCAL1 dynamics,and Fo10 coordinates multiple plant pro-cesses,including Cd uptake,vacuolar sequestration,efflux to the environment,and formation of Fe pla-ques in the rhizosphere.These processes are dependent on the salicylic acid signaling pathway to keep Cd levels low in the cytosol of rice cells and to decrease Cd levels in rice grains without any yield penalty.Fo10 also plays a role in Cd tolerance in the poaceous crop maize and wheat,but has no observed effects in the eudicot plants Arabidopsis and tomato.Taken together,these findings provide insights into the mech-anistic basis underlying how afungal endophyte and host plant interact to control Cd accumulation in host plants by adapting defense responses to promotethe establishment ofa symbiosis that permits adaptation to high-Cd environments.

关键词： defensin-like protein Fusarium oxysporum endophyte cadmium allocation

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Dietary butyrate,lauric acid and stearic acid improve gut morphology and epithelial cell turnover in weaned piglets

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Animal Nutrition 2022年第4期11卷 276-282页

作者： Xianglin Zeng Yuan Yang junmin wang Zhaobin wang Jun Li Yulong Yin Huansheng Yang Hunan International Joint Laboratory of Animal Intestinal Ecology and Health Animal Nutrition and Human Health LaboratorySchool of Life SciencesHunan Normal UniversityChangshaHunan 410081China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical RegionHunan Provincial Engineering Research Center of Healthy LivestockScientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangshaHunan 410125China State Key Laboratory of Food Safety Technology for Meat Products Yinxiang GroupFujian Aonong Biological Science and Technology Group Co.Ltd.Key Laboratory of Swine Nutrition and Feed Science of Fujian ProvinceAonong GroupZhangzhouFujian 363000China College of Pharmacy Changsha Medical UniversityChangshaHunan410219China

This study was to evaluate the effects of the supplementation of saturated fatty acids with different chain lengths on growth performance,intestinal morphology,epithelial cell proliferation,differentiation and apoptosis in weaned piglets.Thirty-two weaned piglets(Duroc×Landrace×Yorkshire,BW=7.81±0.26 kg)were weaned at 21 d and randomly assigned to 1 of 4 experimental treatments:(1)a basal diet(control);(2)control+0.3% butyrate(BT);(3)control+0.3%lauric acid(LA);(4)control+0.3% stearic acid(SA).All piglets were then slaughtered for tissue sampling after having been fed experimental diets for 28 d after weaning.Supplementation of BT increased the gain-to-feed ratio(G:F)(P<0.05)compared to piglets fed the control diet from 14 to 28 d.In addition,the villus height(VH)to crypt depth(CD)ratio(VH:CD ratio)of the ileum were higher in the BT and LA diets than that of the control diet(P<0.05).The SA-supplemented diet increased ileal VH(P<0.05),whereas the BT-supplemented diet increased jejunal CD(P<0.05).Compared to the control,diets supplemented with BT,LA,or SA all tended to increase jejunal proliferation(Ki67/crypt positive cells)(P=0.190);diets supplemented with BT or SA significantly increased the number of ki67-positive cells in the ileal crypt(P<0.05).Furthermore,in the jejunum,the protein expression of activated caspase 3 and villin were increased in piglets fed BT,LA,or SA diets compared to those on the control diet(P<0.05).In the ileum,compared with the control diet,the BT diet tended to increase the protein level of mammalian phosphorylation target of rapamycin(p-m TOR,P<0.10);LA or SA diets significantly increased p-m TOR protein expression(P<0.05).These results show that dietary supplementation of BT,LA,or SA promotes jejunal cell renewal in weaned piglets.At the same time,increased proliferation of ileal crypt cells by promoting p-m TOR expression has beneficial effects on ileal morphology in weaned piglets.

关键词： Weaned piglet Saturated fatty acid Growth performance Intestinal morphology Proliferation

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Stabilization of the Cascaded ODE-Schrödinger Equations Subject to Observation With Time Delay

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IEEE/CAA Journal of Automatica Sinica 2019年第4期6卷 1027-1035页

作者： Aye Aye Than junmin wang the School of Mathematics and Statistics Beijing Institute of TechnologyBeijing 100081China IEEE

This paper focuses on the stabilization of the cascaded Schrodinger-ODE equations subject to the observation with time delay.Both observer and predictor systems are designed to estimate the state variable on the time interval[0,t-τ]when the observation is available,and to predict the state variable on the time interval[t-τ,t]when the observation is not available,respectively.Based on the estimated state variable and the output feedback stabilizing controller using the backstepping method,it is shown that the closed-loop system is exponentially stable.

关键词： Backstepping observer and predictor ODE-Schrödinger system stability time delay

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Boundary feedback stabilization of a Schrdinger equation interconnected with a heat equation

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控制理论与应用（英文版） 2013年第4期11卷 558-562页

作者： Junjun LIU junmin wang School of Mathematics Beijing Institute of Technology

In this paper, we study stabilization for a Schoedinger equation, which is interconnected with a heat equation via boundary coupling. A direct boundary feedback control is adopted. By a detailed spectral analysis, it is found that there are two branches of eigenvalues： one is along the negative real axis, and the other is approaching to a vertical line, which is parallel to the imagine axis. Moreover, it is shown that there is a set of generalized eigenfunctions, which forms a Riesz basis for the energy state space. Finally, the spectrum-determined growth condition is held and the exponential stability of the system is then concluded.

关键词： Schroedinger equation Heat equation Boundary control Stability Riesz basis

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新西兰学前儿童社会情感能力培养:指导框架与项目实践

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学前教育研究 2024年第3期 12-24页

作者：王俊民袁瑀重庆师范大学初等教育学院重庆400700 怀卡托大学教育学部新西兰汉密尔顿3240

为提高儿童幸福感和社会包容性,落实国家课程有关社会情感能力的学习结果,在国家课程倡导有意识教学的背景下,新西兰教育部于2019年发布指南文件《在早期学习中支持社会情感能力》,为学前教育提供指导框架,支持教师有目的、有计划地培... 详细信息

为提高儿童幸福感和社会包容性,落实国家课程有关社会情感能力的学习结果,在国家课程倡导有意识教学的背景下,新西兰教育部于2019年发布指南文件《在早期学习中支持社会情感能力》,为学前教育提供指导框架,支持教师有目的、有计划地培养儿童的社会情感能力。2020年,在前期项目实践的基础上,新西兰政府发起三个专门针对学前教育的社会情感学习试点项目,对教师和家长进行理论培训与实践指导,取得了良好效果。在追求高质量发展的背景下,新西兰学前教育正在寻求儿童主导学习和教师主导学习之间的一种平衡;通过提供策略和实践指导,可以提升教师有意识地开展社会情感学习的信心和实践能力,改善实践效果。我国儿童社会情感能力培养实践可借鉴国际理论成果和优秀实践经验,关注有意识教学和文化响应实践,加强对教师和家长实践能力的培训。

关键词：新西兰学前教育社会情感能力有意识教学文化响应实践

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新西兰新一轮基础教育课程改革:背景、内容与特点

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比较教育研究 2024年第3期46卷 43-51页

作者：王俊民袁瑀重庆师范大学初等教育学院重庆400700 怀卡托大学教育学部新西兰汉密尔顿3240

2018年,新西兰启动21世纪第二轮基础教育课程改革,自2023年初开始分批次实施新课程。本次课程改革是基于核心素养的《新西兰课程》实施十年后,在政府倡导高质量、全纳的公共教育体系背景下展开。新课程延续了“学习者中心”的课程价值取... 详细信息

2018年,新西兰启动21世纪第二轮基础教育课程改革,自2023年初开始分批次实施新课程。本次课程改革是基于核心素养的《新西兰课程》实施十年后,在政府倡导高质量、全纳的公共教育体系背景下展开。新课程延续了“学习者中心”的课程价值取向,强调包容性和个性化学习;将毛利知识作为独立的知识体系全面融入各学习领域,强调双文化整合与身份认同,表现出明显的“本土化”趋势;重构“理解、知道、做”的内容模型和基于学习进阶的学业表现,强化学习领域与课程愿景、核心素养之间的关系,强调通过学科实践和社会情感学习促进核心素养的落实,表现出从“灵活”走向“明确”的趋势和学科实践取向。

关键词：新西兰基础教育课程改革核心素养双文化课程

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Stability analysis for an Euler-Bernoulli beam under local internal control and boundary observation

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控制理论与应用（英文版） 2008年第4期6卷 341-350页

作者： junmin wang Baozhu GUO Kunyi YANG Department of Mathematics Beijing Institute of Technology Beijing 100081 China Academy of Mathematics and Systems Science Academia Sinica Beijing 100080 China

An Euler-Bernoulli beam system under the local internal distributed control and boundary point observation is studied. An infinite-dimensional observer for the open-loop system is designed. The closed-loop system that is non- dissipative is obtained by the estimated state feedback. By a detailed spectral analysis, it is shown that there is a set of generalized eigenfunctions, which forms a Riesz basis for the state space. Consequently, both the spectrum-determined growth condition and exponential stability are concluded.

关键词： Euler-Bernoulli equation Observer Riesz basis Controllability and observability Stability

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End-to-End Fusion of Hyperspectral and Chlorophyll Fluorescence Imaging to Identify Rice Stresses

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Plant Phenomics 2022年第1期4卷 142-155页

作者： Chu Zhang Lei Zhou Qinlin Xiao Xiulin Bai Baohua Wu Na Wu Yiying Zhao junmin wang Lei Feng School of Information Engineering Huzhou UniversityHuzhou 313000China College of Mechanical and Electronic Engineering Nanjing Forestry UniversityNanjingChina College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China Key Laboratory of Spectroscopy Sensing Ministry of Agriculture and Rural AffairsHangzhou 310058China Institute of Digital Agriculture Zhejiang Academy of Agricultural SciencesHangzhou 310021China Institute of Crop Science and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021China

Herbicides and heavy metals are hazardous substances of environmental pollution,resulting in plant stress and harming humans and animals.Identification of stress types can help trace stress sources,manage plant growth,and improve stress-resistant breeding.In this research,hyperspectral imaging(HSI)and chlorophyll fluorescence imaging(Chl-FI)were adopted to identify the rice plants under two types of herbicide stresses(butachlor(DCA)and quinclorac(ELK))and two types of heavy metal stresses(cadmium(Cd)and copper(Cu)).

关键词： breeding. Fluorescence pollution

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Denitrification and phosphorus uptake by DPAOs using nitrite as an electron acceptor by step-feed strategies

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Frontiers of Environmental Science & Engineering 2013年第2期7卷 267-272页

作者： Bin MA Shuying wang Guibing ZHU Shijian GE junmin wang Nanqi Ren Yongzhen PENG State Key Laboratory of Urban Water Resource and Environment (HIT) Harbin Institute of Technology Harbin 150090 China Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering Beijing University of Technology Beijing 100022 China State Key Laboratory of Environmental Aquatic Quality Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

把亚硝酸根用作一个电子领受人的从的磷积累有机体(DPAO ) 能减少更多的精力。然而，亚硝酸根被报导了在从上有抑制磷移动。在这研究，步喂策略被建议完成低亚硝酸根集中，它能避免或减轻亚硝酸根抑制。结果证明那 denitrification 率... 详细信息

把亚硝酸根用作一个电子领受人的从的磷积累有机体(DPAO ) 能减少更多的精力。然而，亚硝酸根被报导了在从上有抑制磷移动。在这研究，步喂策略被建议完成低亚硝酸根集中，它能避免或减轻亚硝酸根抑制。结果证明那 denitrification 率，磷举起率和到亚硝酸根的磷 uptaken 的比率从(缺氧的 P/N 比率) 当亚硝酸根集中是 15 mg 时。

关键词：反硝化聚磷亚硝酸盐电子受体吸收利用饲料反硝化除磷 NO2-N 浓度增加

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相关专业培养小学科学教师的政策逻辑、挑战与实现路径

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天津市教科院学报 2024年第1期36卷 3-13页

作者：王俊民重庆师范大学科技教育与传播中心重庆400700

加大相关专业科学教师人才培养力度是我国加强小学科学教师培养的一项重要举措。但数学与应用数学、物理学等相关专业的人才培养目标和课程设置各不相同,培养小学科学教师的优势和面临的挑战差异较大。培养高素质专业化创新型小学科学... 详细信息

加大相关专业科学教师人才培养力度是我国加强小学科学教师培养的一项重要举措。但数学与应用数学、物理学等相关专业的人才培养目标和课程设置各不相同,培养小学科学教师的优势和面临的挑战差异较大。培养高素质专业化创新型小学科学教师背景下,相关专业应在地方政策协调与调研的基础上,基于专业优势,以直接培养、第二学位、辅修专业、选修课程、中小学合作项目、特殊培养计划等多种方式参与小学科学教师培养,同时通过宣传加强理工科大学生对小学科学教学工作的了解和对小学科学教师身份的认同。我国可借鉴国际经验,设置一年制教学学士后文凭课程,为有志于从事小学科学教学工作的理工科人才提供入职通道。

关键词：小学科学教师教师培养专业化政策

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