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Influence of Different Sterilization Conditions on the Growth and Exopolysaccharide of Streptococcus thermophilusand Co-Cultivation with Lactobacillus delbrueckiisubsp. bulgaricusOLL1073R-1

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Advances in Microbiology 2015年第11期5卷 760-767页

作者： junko nishimura Seiya Makino Katsunori Kimura Emiko Isogai Tadao Saito Laboratory of Nutrition and Life Sciences Department of Biotechnology and Environmental Engineering Faculty of Engineering Hachinohe Institute of Technology Hachinohe Japan Laboratory of Animal Products Chemistry Graduate School of Agricultural Science Tohoku University Sendai Japan Food Science Research Labs. Meiji Co. Ltd. Odawara Japan Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University Sendai Japan

The sterilization conditions between experiment and dairy industrial level are different and concern about influence on product’s qualities. In this study, the fermentation properties of Streptococcus thermophilus, alone and in combination with Lactobacillus delbrueckii subsp. bulgaricus, were evaluated in skim milk that had been subjected to distinct sterilization conditions. Growth, organic acid generation, and EPS production were determined using pasteurized or autoclaved milk. When S. thermophilus was cultivated in pasteurized skim milk, the growth was strain-dependent. On the other hand, growth of S. thermophilus was accelerated in autoclaved milk. Exocellular polysaccharide (EPS) production by L. bulgaricus was not affected by the combination of S. thermophilus strains. Thus, we observed that yogurt fermented by L. bulgaricus was minimally affected by the combination of S. thermophilus strains;growth of L. bulgaricus was maintained under the constant environment. These results should facilitate the development of fermented milk produced from L. bulgaricus in the dairy industry.

关键词： Streptococcus thermophilus Lactobacillus delbrueckii subsp. bulgaricus Sterilization Formic Acid Exopolysaccharide

Risk factors for deterioration of long-term liver function after radiofrequency ablation therapy

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World Journal of Hepatology 2016年第13期8卷 597-604页

作者： Koichi Honda Masataka Seike Junya Oribe Mizuki Endo Mie Arakawa Hiroki Syo Masao Iwao Masanori Tokoro junko nishimura Tetsu Mori Tsutomu Yamashita Satoshi Fukuchi Toyokichi Muro Kazunari Murakami Department of Gastroenterology Faculty of Medicine Oita University Department of Gastroenterology and Hepatology National Hospital Organization Oita Medical Center

AIM:To identify factors that influence long-term liver function following radiofrequency ablation(RFA)in patients with viral hepatitis-related hepatocellular carcinoma.METHODS:A total of 123 patients with hepatitis B virus-or hepatitis C virus-related hepatocellular carcinoma(HCC)(n=12 and n=111,respectively)were enrolled.Cumulative rates of worsening Child-Pugh(CP)scores(defined as a 2-point increase)were examined.RESULTS:CP score worsening was confirmed in 22patients over a mean follow-up period of 43.8±26.3mo.Multivariate analysis identified CP class,platelet count,and aspartate aminotransferase levels as significant predictors of a worsening CP score(P=0.000,P=0.011 and P=0.024,respectively).In contrast,repeated RFA was not identified as a risk factor for liver function deterioration.CONCLUSION:Long-term liver function following RFA was dependent on liver functional reserve,the degreeof fibrosis present,and the activity of the hepatitis condition for this cohort.Therefore,in order to maintain liver function for an extended period following RFA,suppression of viral hepatitis activity is important even after the treatment of HCC.

关键词： Radiofrequency ablation Hepatocellular carcinoma Liver function Hepatitis B Hepatitis C

Biofilm Formation by Streptococcus mutansand Related Bacteria

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Advances in Microbiology 2012年第3期2卷 208-215页

作者： junko nishimura Tadao Saito Hiroshi Yoneyama Lan Lan Bai Kazuhiko Okumura Emiko Isogai Department of Oral Surgery Health Sciences University of Hokkaido Hokkaido Japan Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University Sendai Japan Laboratory of Animal Products Chemistry Graduate School of Agricultural Science Tohoku University Sendai Japan

Caries is a disease of human dentition characterized by the loss of mineralized surfaces of the tooth;it is an infectious disease of the oral cavity in which biofilms play a causative role. Control of biofilms has traditionally relied on non-specific removal of plaque by mechanical means such as brushing, although it is difficult to remove biofilms by this method. Caries is also a widespread infection in children. Streptococcus mutans and S. sobrinus are important causative agents of caries. They produce a homologous exocellular polysaccharide called glucan, which strongly adheres to the enamel surface. This is a review of oral microbial biofilm formation by S. mutans and other related bacteria.

关键词： Caries Streptococcus mutans Streptococcus sobrinus Biofilm Exocellular Polysaccharide Glucan Glucosyltransferase

Survival, Proliferation and Cell Cycle of Swine Fibroblast after Infection with Salmonella enterica

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Advances in Microbiology 2016年第13期6卷 942-952页

作者： Mizuki Masuda Yijie Guo Kengo Kuroda Jun Xu Hiroshi Yoneyama Tomokazu Fukuda Bernard Mudenda Hang’ombe Katsuya Okuno junko nishimura Emiko Isogai Laboratory of Animal Microbiology Graduate School of Agricultural Science Tohoku University Sendai Japan Department of Immunobiology and Pathogenic Biology Medical School of Xi’an Jiaotong University Xi’an China Laboratory of Cell Engineering and Molecular Genetics Faculty of Science and Engineering Iwate University Morioka Japan Department of Clinical Microbiology School of Veterinary Medicine University of Zambia Lusaka Zambia Laboratory of Nutrition and Life Sciences Department of Biotechnology and Environmental Engineering Faculty of Engineering Hachinohe Institute of Technology Hachinohe Japan

Food-borne salmonellosis continues to be a major health concern worldwide. Carry-contamination of Salmonella frequently occurs in meat production. We focused on cell dynamics of swine fibroblasts after infection with Salmonella enterica serovar Enteritidis and Typhimurium, because fibroblast can be a target cell for Salmonella latent infection. It was found that both S. Enteritidis and S. Typhimurium were able to adhere and invade to swine fibroblasts. The proliferations in fibroblasts were different between each serovar. S. Enteritidis reached to the maximum at 24 hr after infection while S. Typhimurium did not. In addition, the decrease in the G0/G1 phase cells and increase in G2/M phase cells on the fibroblast were observed by both Salmonella infection. Cell death including apoptosis in the cells was inhibited by the infection of Salmonella. These results suggest that nontyphoidal Salmonella can survive for the long term by modifying bacterial cell proliferation and preventing cell death of host cells.

关键词： Salmonella enterica Fibroblast Swine Cell Death Cell Cycle

Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA Gene Sequences

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Advances in Microbiology 2014年第13期4卷 857-870页

作者： I-Nung Huang Yuri Sato Mitsuo Sakamoto Moriya Ohkuma Shinobu Ohnuma Takeshi Naitoh Chikashi Shibata Akira Horii junko nishimura Haruki Kitazawa Tadao Saito Graduate School of Agricultural Science Tohoku University Miyagi Japan Japan Collection of Microorganisms RIKEN BioResource Center Ibaraki Japan Department of Surgery Tohoku University Graduate School of Medicine Miyagi Japan Department of Molecular Pathology Tohoku University Graduate School of Medicine Miyagi Japan

Although the gut microflora is thought to be an essential factor in the development of ulcerative colitis (UC), the entire gut microflora occurring in UC remains unknown. Most studies use feces to represent the microflora distribution;however, here we analyzed the bacterial diversity in colonic mucus from UC patients receiving colectomy surgery and control patients. The diversity of microflora was investigated using a combination of terminal restriction fragment length polymorphism (T-RFLP) and clone library analyses of the 16S rRNA gene sequences. In the T-RFLP analysis, the number of terminal restriction fragments (T-RFs) decreased significantly in UC patients when compared to control samples. Also in the clone library analysis, the number of operational taxonomic units (OTU) and the Shannon diversity index were reduced significantly in UC patients. These molecular analyses reveal an overall dysbiosis in UC patients. No specific pathogen was found, and a strong negative correlation in relative abundance of bacterial populations was observed between the phyla Bacteroidetes and Firmicutes in the UC patients. This is the first report showing a significant correlation between these two phyla, which may be important characteristics in the pathogenesis of UC.

关键词： Ulcerative Colitis Microflora Terminal Restriction Fragment Length Polymorphism 16S rRNA Gene Clone Library

桑蚕(Bombyx mori)发育中成虫脑的促前胸腺激素(PTTH)的部分提纯及其性质

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中国蚕业 1986年第1期 29-34页

作者： junko Nishitsutsuji-Uwo Masaji S. nishimura 华跃进

材料和方法 PTTH提取物的来源: 把种和雌雄混合的桑蚕(Bombys mori)潜成虫(phaiute adult)(蛹)用干冰冷冻2～3天,解剖下来的脑迅速浸入冷丙酮(-20℃)中,换液6～10次,直到提取液无色,贮存到冰箱内(-20℃)。 PTTH的生物测定: 用蓖麻蚕去... 详细信息

材料和方法 PTTH提取物的来源: 把种和雌雄混合的桑蚕(Bombys mori)潜成虫(phaiute adult)(蛹)用干冰冷冻2～3天,解剖下来的脑迅速浸入冷丙酮(-20℃)中,换液6～10次,直到提取液无色,贮存到冰箱内(-20℃)。 PTTH的生物测定: 用蓖麻蚕去脑蛹。如结果所详述的,用在每一纯化过程中使用的去离子水或缓冲剂

关键词：层析柱溶液平衡葡聚糖制剂 DEAE PTTH Bombyx mori 蓖麻蚕鹤木蚕有效剂量活性组分桑蚕家蚕