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二联体亲子鉴定罕见案例分析及应对策略

西南医科大学学报 2024年第3期47卷 231-235页

作者：郭侃付杰文宋炳辉钱捷罗章成竞梁何涛傅俊江西南医科大学医学基础研究中心表观遗传与肿瘤四川省高校重点实验室泸州646000 西南医科大学司法鉴定中心DNA法医物证实验室泸州646000

目的对亲子鉴定中二联体罕见案例进行分析并给出应对策略。方法对罕见案例样本进行DNA提取,使用AGCU EX-22试剂盒进行二联体和三联体检测;使用阅微基因36A试剂盒进行二联体检测。通过遗传测序仪进行电泳分析,GeneMapper ID-X软件进行数... 详细信息

目的对亲子鉴定中二联体罕见案例进行分析并给出应对策略。方法对罕见案例样本进行DNA提取,使用AGCU EX-22试剂盒进行二联体和三联体检测;使用阅微基因36A试剂盒进行二联体检测。通过遗传测序仪进行电泳分析,GeneMapper ID-X软件进行数据分析得到短串联重复序列(short tandem repeats,STR)图谱和相应的基因座数据,并计算其累积亲权指数。结果采用AGCU EX-22试剂盒进行被检父和孩子二联体鉴定,获得累积亲权指数为7.0516×10^(2),这个值既大于0.0001又小于10000。根据《亲权鉴定技术规范》(GB/T 37223-2018),其结果是既不支持、又不能排除被检父为孩子的生物学父亲,不能出具明确的鉴定意见;增加孩子母亲样本,改为三联体鉴定后,使用相同的试剂盒进行鉴定,获得的累积亲权指数为2.6327×10^(8),大于10000,支持被检父为孩子的生物学父亲。与此同时,增加STR基因座位点,使用阅微基因36A试剂盒进行被检父和孩子的二联体鉴定,获得累积亲权指数为1.0378×10^(9),同样大于10000,亦能出具明确的鉴定意见。结论在二联体亲子鉴定中,通过增加孩子生母做三联体亲子鉴定或者是增加STR位点进行检测,都能提高累积亲权指数,帮助罕见疑难亲子鉴定案例出具明确的鉴定意见。

关键词：短串联重复序列二联体亲权鉴定三联体亲权鉴定累积亲权指数

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通过改良RAPD技术获得黑毛石斛、蜂腰石斛和石仙桃的3条SCAR新标记

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西南医科大学学报 2024年第1期47卷 29-33页

作者：梅志强郭侃魏春莉郑美玲傅俊江西南医科大学医学基础研究中心泸州646000

目的寻找快速区分黑毛石斛、蜂腰石斛和石仙桃的序列特征扩增区域(sequence characterized amplified regions,SCAR)新标记。方法在改良的随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)的基础上对10个物种进行基因组DNA随... 详细信息

目的寻找快速区分黑毛石斛、蜂腰石斛和石仙桃的序列特征扩增区域(sequence characterized amplified regions,SCAR)新标记。方法在改良的随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)的基础上对10个物种进行基因组DNA随机扩增、分子克隆和测序,分别设计了可鉴别3个物种的SCAR标记。结果成功筛选出3个SCAR标记,其相应的特异性标记分别为N2-12、A2-17和A6-33;其中SCAR标记N2-12在黑毛种属中扩增出约750 bp的特异性条带,SCAR标记A2-17在蜂腰石斛中扩增出约750 bp的特异性条带,SCAR标记A6-33在石仙桃种属中扩增出约600 bp的特异性条带。结论N2-12、A2-17和A6-33这3对特异性SCAR新标记能快速、准确的区分出黑毛石斛、蜂腰石斛和石仙桃这3个物种,为今后进行该中药道地药材样品的鉴定提供了实验依据。

关键词：黑毛石斛蜂腰石斛石仙桃序列特征扩增区域标记鉴定

Mutant p53 promotes cell spreading and migration via ARHGAP44

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Science China(Life Sciences) 2017年第9期60卷 1019-1029页

作者： Jinjin Xu Jian Jiao Wei Xu Lei Ji Dongjie Jiang Shaofang Xie Syeda Kubra Xiaotao Li junjiang fu Jianru Xiao Bianhong Zhang Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical SciencesSchool of Life SciencesEast China Normal University Department of Orthopedic Oncology Changzheng Hospitalthe Second Military Medical University Key Laboratory of Epigenetics and Oncology The Research Center for Preclinical MedicineSouthwest Medical University

The tumor suppressor p53 protein is either lost or mutated in about half of all human *** of p53 function is well known to influence cell spreading,migration and *** expression of mutant p53 is not equivalent to p53 loss,mutant p53 can acquire new functions to drive cell spreading and migration via different *** our study,we found that mutant p53 significantly increased cell spreading and migration when comparing with p53-null ***-Seq analysis suggested that Rho GTPase activating protein 44(ARHGAP44) is a new target of mutant p53,which suppressed AKHGAP44 ***44 has GAP activity and catalyze GTP hydrolysis on *** level of GTP-Cdc42 was correlated with increase expression of mutant p53 and reduced ***,wt-ARHGAP44 but not mutant ARHGAP44(R291A) suppressed mutant p53 mediated cell spreading and *** analysis indicated lower expression of ARHGAP44 in lung carcinoma compared with normal tissues,which was verified by RT-qPCR using specimens from *** interestingly,ARHGAP44 mRNA level was lower in tumors with mutant p53 than those with normal ***,our results disclose a new mechanism by which mutant p53 stimulates cell spreading and migration.

关键词： mutant p53 novel function of ARHGAP44 cell spreading cell migration cancer new mechanism

Upregulation of IL17RD links to anti-metastatic effect of thymoquinone in triple negative breast cancer cells

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Upregulation of IL17RD links to anti-metastatic effect of th...

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： KHAN,MDASADUZZAMAN ZHENG,MEILING fu,junjiang Southwest Medical University

Triple negative breast cancer (TNBC) is a molecular subtype of breast cancer,which is a major burden on women’s *** is so difficult to develop effective chemotherapeutic drugs against TNBC due to the complex carcinogenic *** previous study [Khan et al.2017;Oncotarget:https://***/10.18632/oncotarget.3973] indicated that natural product thymoquinone is effective against TNBC cells and animal *** this study,

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Linear shallow neural network to accelerate transmitter dispersion eye closure quaternary(TDECQ) assessment

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Science China(Information Sciences) 2024年第4期67卷 337-338页

作者： junjiang XIANG Zejun CHEN Yijun CHENG Hailin YANG Xuancheng HUO Meng XIANG Gai ZHOU Yuwen QIN Songnian fu Institute of Advanced Photonics Technology School of Information EngineeringGuangdong University of Technology School of Optical and Electronic Information Huazhong University of Science and Technology Key Laboratory of Photonic Technology for Integrated Sensing and Communication Ministry of Education of ChinaGuangdong University of Technology Guangdong Provincial Key Laboratory of Information Photonics Technology Guangdong University of Technology

To support the ever-emerging capacity requirement of datacenter interconnection(DCI) applications, transmitter dispersion eye closure quaternary(TDECQ) has been proposed by IEEE802.3 standard [1], which is specifically employed for the four-level pulse amplitude modulation(PAM-4) signals. However, the traditional scheme of TDECQ assessment needs several iterative operations,

关键词： IEEE Standards

Impacts of transmembrane serine protease 4 expression on susceptibility to severe acute respiratory syndrome coronavirus 2

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Chinese Medical Journal 2023年第7期136卷 860-862页

作者： Qi Tan Jiewen fu Zhiying Liu Haoyue Deng Lianmei Zhang Jiayue He Xiaotao Li junjiang fu Key Laboratory of Epigenetics and Oncology The Research Center for Preclinical MedicineSouthwest Medical UniversityLuzhouSichuan 646000China Department of Pathology The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical UniversityHuai’anJiangsu 223300China Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical SciencesSchool of Life SciencesEast China Normal UniversityShanghai 200241China

To the Editor:Transmembrane serine protease 4(TMPRSS4;OMIM:606565),which encodes a member of the serine protease family,is involved in various biological processes related to diseases such as cancer and pediatric neur... 详细信息

关键词： protease diseases respiratory

The Speckle-type POZ protein(SPOP) inhibits breast cancer malignancy by destabilizing TWIST1

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The Speckle-type POZ protein(SPOP) inhibits breast cancer ma...

2022CCTB中国肿瘤标志物学术大会暨中国整合肿瘤学大会暨第十六届肿瘤标志物青年科学家论坛暨中国肿瘤标志物产业创新大会

作者： Chunli Wei junjiang fu Xiaoyan Liu Jingliang Cheng Jiewen fu Southwest Medical University

Epithelial-mesenchymal transition(EMT) inducing transcription factor TWIST1 plays a vital role in cancer *** the tumor-suppressive E3 ligase,Speckle-type POZ protein(SPOP),regulates TWIST1 in breast cancer remains *** this study,we report that SPOP physically interacts with,ubiquitinates,and destabilizes *** promotes K63-and K48-linked ubiquitination of TWIST1,predominantly at K73,thereby suppressing cancer cell migration and *** SPOP significantly enhances EMT,which accelerates breast cancer cell migration and invasiveness in vitro and lung metastasis in ***,SPOP is negatively correlated with the levels of TWIST1 in highly invasive breast *** SPOP expression,along with elevated TWIST1 levels,is associated with poor prognosis in advanced breast cancer patients,particularly those with metastatic triple-negative breast cancer(TNBC).Taken together,we have disclosed a new mechanism linking SPOP to TWIST1 *** SPOP may serve as a prognostic marker and a potential therapeutic target for advanced TNBC patients.

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Exploring the effect of thymoquinone on PI3K/Akt pathway and PIK3CA mutations in breast cancer cells

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Exploring the effect of thymoquinone on PI3K/Akt pathway and...

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： Zhou,Ju Shen,Shiyi Xiao,Ting fu,junjiang Southwest Medical University

[Purpose]17 million new cases of breast cancer each year represent a major health problem,although significant progress has been made in the field of medical *** addition to surgery,the main interventions for breast cancer include chemotherapy and radiotherapy,which are expensive,low selectivity and high side *** with traditional therapy,natural drugs like thmoquinone,

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Thymoquinone inhibits cervical cancer metastasis in vitro by targeting epithelial to mesenchymal transition associated transcription factors Twist1 and Zeb1

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Thymoquinone inhibits cervical cancer metastasis in vitro by...

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Jun Li Asaduzzaman Khan Chunli Wei Jingliang Cheng Hanchun Chen junjiang fu Key Laboratory of Epigenetics and Oncology Research Center for Preclinical MedicineSouthwest Medical University

Cervical cancer is a major type of gynecological malignant tumor, of which the chemotherapeutic strategies are limited by its non-specific cytotoxicity and drug resistance. Thymoquinone(TQ), a natural product has been reported to target vast number of signaling pathways in carcinogenesis in different cancers, and hence regarded as a promising anticancer molecule. Inhibition of epithelial to mesenchymal transition(EMT) regulators is anical cancer cell lines, SiHa and CaSki, to investigate its effects on EMT-regulatory important cancer therapeutic approach. Here TQ was used to treat cervproteins and cancer metastasis. We found that TQ has time-dependent and dose-dependent cytotoxic effect, as well as it inhibits the migration and invasion process in different cervical cancer cells. Mechanistically TQ treatment inhibited the expression of Twist1, Zeb1 expression, and increased E-Cadherin expression. Luciferase reporter assay showed that TQ decreases the Twist1 and Zeb1 promoter activity, indicating that Twist1 and Zeb1 might be the direct target of TQ. TQ also increased cellular apoptosis in some extent, but apoptotic genes/proteins we tested were not affected. We summarize that TQ inhibits the migration and invasion of cervical cancer cells probably via Twist1/ECadherin/EMT or/and Zeb1/E-Cadherin/EMT among other signaling pathways.

关键词： Cervical cancer Metastasis Epithelial to mesenchymal transition Twist1 Zeb1 E-Cadherin Thymoquinone