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Effect of herbal medicine Juzentaihoto on hepatic and intestinal heat shock gene expression requires intestinal microflora in mouse

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World Journal of Gastroenterology 2007年第16期13卷 2289-2297页

作者： Miho Kato Atsushi Ishige Naoko Anjiki Masahiro Yamamoto Yoshifumi Irie Mitsue Taniyama Ryoko Kibe junichiro oka Yoshimi Benno Kenji Watanabe Department of Kampo Medicine Keio University School of Medicine 35 Shinano-machi Shinjuku-ku Tokyo 160-8582 Japan Faculty of Pharmaceutical Science Tokyo University of Science 264 Yamazaki Noda City Chiba 278-8519 Japan Japan Collection of Microorganisms Riken 2-1 Hirosawa Wako-shi Saitama 351-0198 Japan Department of Kampo Medicine Keio University School of Medicine 35 Shinano-machi Shinjuku-ku Tokyo 160-8582 Japan Department of Kampo Medicine Keio University School of Medicine 35 Shinano-machi Shinjuku-ku Tokyo 160-8582 Japan Pharmacology Research Department Tsumura Research Institute Research Division 3586 Yoshiwara Ami-machi Inashiki-gun Ibaraki 300-1192 Japan Pharmacology Research Department Tsumura Research Institute Research Division 3586 Yoshiwara Ami-machi Inashiki-gun Ibaraki 300-1192 Japan Japan Collection of Microorganisms Riken 2-1 Hirosawa Wako-shi Saitama 351-0198 Japan Faculty of Pharmaceutical Science Tokyo University of Science 264 Yamazaki Noda City Chiba 278-8519 Japan

AIM： To evaluate the role of intestinal microflora in the effects of multi-herbal medicine on gene expression in the gut and liver. METHODS： The multi-herbal medicine Juzentaihoto （JTX） was administered to five germ-free mice and regular mice for 2 wk. Among the results of the comprehensive gene chip analysis of the intestine and liver, we featured heat shock proteins （HSPs） 70 and 105 because their gene expression changed only in the presence of microflora. Real-time RT-PCR was performed to confirm the expression levels of these HSP genes. To determine whether JTX acts directly on the HSP genes, sodium arsenite （SA） was used to induce the heat shock proteins directly. To examine the change of the intestinal microflora with administration of JTX, the terminal restriction fragment polymorphism （T-RFLP） method was used. To identify the changed bacteria, DNA sequencing was *** by gene chip and real-time RT-PCR, changed with the administration of JTX in the regular mice but not in the germ-free mice. JTX did not suppress the direct induction of the HSPs by SA. T-RFLP suggested that JTX decreased unculturable bacteria and increased Lactobacillus johnsoni. These data suggested that JTX changed the intestinal microflora which, in turn, changed HSP gene ***： Intestinal microflora affects multi-herbal product JTX on the gene expression in the gut and liver.

关键词： Herbal medicine Juzentaihoto Intestinal microflora Terminal restriction fragment length polymorphism Heat shock protein

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Reactivity improvement of ilmenite by calcium nitrate melt infiltration for Chemical Looping Combustion of biomass

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Carbon Resources Conversion 2019年第1期2卷 51-58页

作者： Martin Keller Hikaru oka junichiro Otomo Graduate School of Frontier Sciences The University of Tokyo5-1-5 KashiwanohaKashiwa-shiChiba 277-8563Japan

Chemical Looping Combustion is a novel process that generates sequestration-ready CO_(2) from the combustion of woody biomass without requiring a gas separation step and without diluting the CO_(2) with N_(2) from *** is achieved by oxidizing the fuel with lattice oxygen of a metal oxide oxygen *** using cheap and abundant ilmenite ore(FeTiO3)as the oxygen carrier,the rather low reactivity towards volatiles released from the biomass upon devolatilization,as well as detrimental structural changes due to a segregation of Fe and Ti in the material,are of *** issues can be addressed by modifying ilmenite with Ca via melt *** this work,we demonstrate that this modification results in a good distribution of Ca throughout the ilmenite particles that a)prevents detrimental Fe/Ti segregation,b)improves the mechanical stability of the particle compared to materials prepared by solution impregnation and c)improves the reactivity of this material towards hydrogen and wet ***,fixed bed experiments showed that the Ca modification not only resulted in increased methane conversion,but also in an increased degree of oxidation of gaseous intermediates CO and *** thus conclude that the performance of ilmenite in Chemical Looping processes can be significantly enhanced by Ca modification of ilmenite either prior to use or in-situ during operation of this bed material with Ca-rich fuels such as woody biomass.

关键词： Carbon dioxide removal Chemical Looping Combustion Ilmenite Melt infiltration BECCS Biomass combustion

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