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The MtRGF6 Peptide Differentially Regulates Root Development and Symbiotic Nodulation of Medicago truncatula and Lotus japonicus

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1237-1248页

作者： junhui yan Yawen Wang Qiong Li Yu Zhou Xu Wang Li Luo School of Life Sciences Shanghai Key Laboratory of Bio-Energy CropsShanghai UniversityShanghai200444China College of Life Science State Key Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefei230036China

Rhizobia induces nitrogen-ﬁxing nodules in legumes used in agricultural production,providing a direct source of combined nitrogen to leguminous crops.Small peptides,such as CLAVATA3/EMBRYO SURROUNDING REGION peptides(CLE),are known to regulate the formation and development of nitrogen-ﬁxing nodules in legumes.Root meristem growth factor(RGF)peptides from Medicago truncatula not only regulate root develop-ment but also modulate nodulation symbiosis with Sinorhizobium meliloti.However,the impact of RGF peptides from one leguminous species on the others remains unclear.In this study,we investigate the effects of the RGF family peptide MtRGF6p from M.truncatula on nodulation symbiosis and root development in Lotus japonicus.The MtRGF6 gene is predominantly expressed in the root nodules of M.truncatula and shows low identity with RGF homologous genes from L.japonicus.The gene promoter is active in the primordia of root nodules and lat-eral roots,as well as in young nodules and roots,and the meristem,infection,and nitrogen-ﬁxing regions of the mature nodule.Chemically synthesized MtRGF6p promoted primary root growth in M.truncatula but sup-pressed the growth of L.japonicus primary roots.The peptide negatively affected the initiation of nodule primor-dia,the formation of infection threads,and nodulation in both legumes,with a low dosage showing effects on L.japonicus compared to M.truncatula.These results suggest that the MtRGF6 peptide from M.truncatula may serve as an inter-species signal affecting the root organ development of L.japonicus.

关键词： Peptide RGF legume symbiotic nodulation lateral root

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Late Holocene brGDGTs-based quantitative paleotemperature reconstruction from lacustrine sediments on the western Tibetan Plateau

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Frontiers of Earth Science 2023年第4期17卷 997-1011页

作者： Xiumei LI Sutao LIU Juzhi HOU Zhe SUN Mingda WANG Xiaohuan HOU Minghua LIU junhui yan Lifang ZHANG Henan Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution School of Geographic SciencesXinyang Normal UniversityXinyang 464000China Institute of Tibetan Plateau Research Chinese Academy of SciencesBeijing 100101China Institute of Geography and Resources Science Sichuan Normal UniversityChengdu 610066China School of Geography Liaoning Normal UniversityDalian 116029China

We present a quantitative mean annual air temperature(MAAT)record spanning the past 4700 years based on the analysis of branched glycerol dialkyl glycerol tetraethers(brGDGTs)from a sediment core from Xiada Co,an alpine lake on the western Tibetan Plateau(TP).The record indicates a relatively stable and warm MAAT until 2200 cal yr BP;subsequently,the MAAT decreased by~4.4℃ at~2100 cal yr BP and maintained a cooling trend until the present day,with centennial-scale oscillations centered at~800 cal yr BP,~600 cal yr BP,and~190-170 cal yr BP.MAAT decreased abruptly at~500-300 cal yr BP and reached its minimum for the past 4700 years.We assessed the representativeness of our record by comparing it with 15 published paleotemperature records from the TP spanning the past~5000 years.The results show divergent temperature variations,including a gradual cooling trend,a warming trend,and no clear trend.We suggest that these discrepancies could be caused by factors such as the seasonality of the temperature proxies,the length of the freezing season of the lakes,the choice of proxy-temperature calibrations,and chronological errors.Our results highlight the need for more high-quality paleotemperature reconstructions with unambiguous climatic significance,clear seasonality,site-specific calibration,and robust dating,to better understand the processes,trends,and mechanisms of Holocene temperature changes on the TP.

关键词： Tibetan Plateau lake sediments branched GDGTs paleotemperature

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Phytosulfokine-α Promotes Root Growth by Repressing Expression of Pectin Methylesterase Inhibitor (PMEI) Genes in Medicago truncatula

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Phyton-International Journal of Experimental Botany 2020年第4期89卷 873-881页

作者： Liangliang Yu Wenjun Zhou Danping Zhang junhui yan Li Luo Shanghai Key Lab of Bio-Energy Crops School of Life SciencesShanghai UniversityShanghai200444China

Phytosulfokine-α(PSK-α),a sulfated pentapeptide with the sequence YIYTQ,is encoded by a small precursor gene family in Arabidopsis.PSK-αregulates multiple growth and developmental processes as a novel peptide hormone.Despite its importance,functions of PSK-αin M.truncatula growth remains unknown.In this study,we identified five genes to encode PSK-αprecursors in M.truncatula.All of these precursors possess conserved PSK-αsignature motif.Expression pattern analysis of these MtPSK genes revealed that each gene was expressed in a tissue-specific or ubiquitous pattern and three of them were remarkably expressed in root.Treatment of M.truncatula seedlings with synthetic PSK-αpeptide significantly promoted root elongation.In addition,expression analysis of downstream genes by RNA-seq and qRT-PCR assays suggested that PSK-αsignaling might regulate cell wall structure via PMEI-PME module to promote root cell growth.Taken together,our results shed light on the mechanism by which PSK-αpromotes root growth in M.truncatula,providing a new resource for improvement of root growth in agriculture.

关键词： Phytosulfokine-α PSK-α PMEI root growth Medicago truncatula

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黄河中上游极端降水特征及其对区域气候变化的敏感性

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资源科学 2024年第3期46卷 524-537页

作者：贺山峰陈超冰李铮冯爱青闫军辉吴绍洪曲阜师范大学地理与旅游学院日照276800 国家气候中心北京100081 信阳师范大学地理科学学院信阳464000 中国科学院地理科学与资源研究所北京100101

[目的]明晰气候变化背景下极端降水事件的区域性特征及变化趋势,对于保障资源环境安全和防灾减灾具有重要意义。[方法]基于1961-2020年气象观测数据,应用线性趋势、M-K检验、小波分析和相关分析等方法,分前期(1961-1990年)和后期(1991-2... 详细信息

[目的]明晰气候变化背景下极端降水事件的区域性特征及变化趋势,对于保障资源环境安全和防灾减灾具有重要意义。[方法]基于1961-2020年气象观测数据,应用线性趋势、M-K检验、小波分析和相关分析等方法,分前期(1961-1990年)和后期(1991-2020年)揭示了黄河中上游极端降水事件的时空分异特征及其对区域气候变化的敏感性。[结果]①除持续干旱日数连续减少,其他极端降水指数均先下降后上升,趋势反转主要发生在1990年代,并且在后期,日降水强度、极端降水量和暴雨日数的上升速率分别为0.43mm/(d·10a)、13.98mm/10a和0.06d/10a,趋势均达到显著水平(p<0.05)。②全时段内研究区西南部较为湿润、河套平原最为干旱,极端强降水呈现东南多、西部少的空间格局。在后期,黄河上游地区暖湿化趋势逐渐增强,中游地区极端强降水的发生频率和强度显著增加。③近60年研究区年均气温上升约1.5℃,升温速率逐渐加快,年降水量先降后升且后期上升趋势达到极显著水平(p<0.01),气候整体上从前期的暖干化向后期的暖湿化转变,尤以黄河上游地区更为明显。极端降水事件变化与区域年均气温表现出较弱的线性对应关系,但对年降水量变化具有较强的敏感性,除持续干早旱日数与年降水量呈弱负相关外,其他极端降水指数与年降水量呈极显著的正相关关系。[结论]极端降水在前期和后期的变化趋势和幅度远大于全时段的变化。1990年代以来,黄河上游暖湿化趋势明显,中游地区极端强降水事件显著增加,未来需重点关注极端降水可能引发的洪涝灾害。

关键词：极端降水时空分异特征气候变化敏感性 Mann-Kendall检验小波分析黄河中上游

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Study on the extremely cold winter of 1670 over the middle and lower reaches of the yangtze River

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Research in Cold and Arid Regions 2014年第6期6卷 540-545页

作者： junhui yan MingQi Li HaoLong Liu JingYun Zheng Hui Fu Key Laboratory of Land Surface Pattern and Simulation Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences College of Urban and Environmental Sciences Xinyang Normal University College of Geography and Tourism Chongqing Normal University

The snow-cover days over the middle and lower reaches of the yangtze River （MLRYR） in the winter of 1670 were extracted from Chinese historical documents. By these records, the winter temperature anomalies （compared to the mean of 1961-1990） recorded at seven meteorological stations and the regional mean winter temperature were estimated. The results show that：（1） There was an average of about 30 snow-cover days over the MLRYR region in 1670, ranging from 11-20 days in Shanghai and eastern Zhejiang to 5140 days in eastern Hunan Province. The snow-cover days averaged about 40 days in Anqing and Nan- cheng, and ranged from 30 to 40 days in Quzhou, Jingdezhen, and Nanchang; and （2） the regional mean winter temperature in 1670 was estimated to be approximately 4.0 ℃ lower than that of 1961-1990. The maximum negative anomaly of 5.6℃ occurred in Nanchang and the minimum anomaly of-2.8 ℃ was detected in Quzhou. Both of these were lower than that of the coldest winter during the instrumental observation period of 1951-2010. This research could not only provide a method to es- timate historical climate extremes, but also provide a background to understand the recent instrumentally climate extremes.

关键词： extreme cold winter snow-cover days Chinese historical documents the middle and lower reaches of the yangtze River the winter of 1670

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苏通GIL综合管廊工程GIL管道专用运输机具研发

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起重运输机械 2024年第11期 76-82页

作者：贾文强闫俊慧宋帅太原学院太原030032 太原重工股份有限公司技术中心太原030032

针对某特高压综合管廊过江输电项目,文中采用盾构管廊方式,管廊两侧分别设3相GIL输电管路,从江底穿长江主航道,两侧通过竖井和地面引接站与架空线路相连,在综合管廊路径长、走向复杂;内部作业空间受限,施工量大且工期紧的情况下,实现了... 详细信息

针对某特高压综合管廊过江输电项目,文中采用盾构管廊方式,管廊两侧分别设3相GIL输电管路,从江底穿长江主航道,两侧通过竖井和地面引接站与架空线路相连,在综合管廊路径长、走向复杂;内部作业空间受限,施工量大且工期紧的情况下,实现了采用过江管廊GIL的方式长距离输电。

关键词：管道专用运输机具综合管廊工程 GIL创新工程长距离输电

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真武汤合肾着汤加减治疗特发性膜性肾病合并急性肾损伤临床观察

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中国中医急症 2024年第5期33卷 852-856页

作者：闫俊慧张乐刘育硕李建军北京大学第三医院秦皇岛医院河北秦皇岛066000

目的观察真武汤合肾着汤加减对特发性膜性肾病(IMN)合并急性肾损伤(AKI)的临床疗效并探讨其作用机制。方法106例患者随机分为对照组与观察组各53例,对照组采用西医综合治疗,观察组在对照组基础上加用真武汤合肾着汤加减内服,连续治疗4... 详细信息

目的观察真武汤合肾着汤加减对特发性膜性肾病(IMN)合并急性肾损伤(AKI)的临床疗效并探讨其作用机制。方法106例患者随机分为对照组与观察组各53例,对照组采用西医综合治疗,观察组在对照组基础上加用真武汤合肾着汤加减内服,连续治疗4周。比较两组治疗前后血肌酐(Scr)、尿素氮(BUN)、肌酐清除率(Ccr)、24 h尿蛋白定量(24 h-UTP)、血清白蛋白(ALB)、三酰甘油(TAG)、总胆固醇(TC)等肾功能和血脂指标。比较治疗前后血液同型半胱氨酸(Hcy)水平、尿液N-乙酰-β-D氨基葡萄糖糖苷酶(NAG)、尿β2-微球蛋白(β2-MG)和肾损伤分子1(Kim-1)水平。比较血清抗磷脂酶A2受体(PLA2R)抗体水平、免疫功能指标外周血T细胞亚群(CD4+、CD8+及CD4+/CD8+)水平、炎症因子白细胞介素-17(IL-17)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和白细胞介素-35(IL-35)水平以及脾肾阳虚证评分。比较治疗后临床总有效率。结果两组治疗前各项指标比较差别均不大(均P>0.05)。两组治疗后,24 h-UTP、Scr、BUN、TAG、TC、Hcy、NAG、β2-MG、Kim-1和抗PLA2R抗体水平下降(均P<0.05),Ccr和ALB水平升高(均P<0.05),且均以观察组为优(均P<0.05)。两组治疗后脾肾阳虚证各主要症状评分及总积分降低(均P<0.05),且观察组低于对照组(P<0.05)。观察组治疗后IL-35、CD4+、CD4+/CD8+均高于对照组(均P<0.05),TNF-α、IL-1β、IL-17、CD8+水平则均低于对照组(均P<0.05)。观察组总有效率为92.45%,高于对照组的75.47%(P<0.05)。结论真武汤合肾着汤加减治疗IMN合并AKI临床疗效显著,其可调节免疫炎症因子,抑制抗PLA2R抗体,减轻肾损伤和蛋白尿,保护肾功能,改善临床症状。

关键词：特发性膜性肾病急性肾损伤真武汤肾着汤抗磷脂酶A2受体(PLA2R)抗体免疫功能炎症因子

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Reconstruction of Sub-Decadal Winter Half-Year Temperature during 1651-2010 for the North China Plain Using Records of Frost Date

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Atmospheric and Climate Sciences 2014年第2期4卷 211-218页

作者： junhui yan Quansheng Ge Haolong Liu Jingyun Zheng Hui Fu Chongqing Normal University Chongqing China Institute of Geographic Sciences and Natural Resources Research (IGSNRR) Chinese Academy of Sciences (CAS) Beijing China

We extracted 374 pieces of records of frost date from historical documents. Using these records, we reconstructed winter half-year (October to next April) temperature series, with a resolution of 5-year, for the North China Plain during 1651-2010. The results show that the temperature changes in the North China Plain were divided into four phases. With the reference period of 1951-1980, two cold phases, 1651-1700 and 1781-1900, have cold anomaly of 0.83°C and 0.60°C respectively. However, between the two cold phases, 1701-1780 was a relative warm phase with the cold anomaly of 0.36°C referring to the mean of 1951-1980. After the 1900, the climate came into a warm phase. The mean temperature of 1901-2010 was 0.11°C higher than the mean of 1951-1980. During 1651-2010, the 1996-2000 is the warmest 5-year with the warm anomaly of 1.25°C than that of the reference period of 1951-1980.

关键词： Historical Climatology Temperature Reconstruction Winter Half-Year The North China Plain 1651-2010

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Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish

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Journal of Integrative Agriculture 2024年第5期23卷 1557-1567页

作者： Jiali Ying yan Wang Liang Xu Tiaojiao Qin Kai Xia Peng Zhang Yinbo Ma Keyun Zhang Lun Wang junhui Dong Lianxue Fan Yuelin Zhu Liwang Liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization/Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of Ministry of Agriculture and Rural Affairs/College of Horticulture Nanjing Agricultural UniversityNanjing210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China

Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification in modern plant biotechnology.However,the investigation of gene silencing and editing in radish remains limited.In this study,a bleaching phenotype was generated through the knockdown of RsPDS using tobacco rattle virus(TRV)-and turnip yellow mosaic virus(TYMV)-mediated gene silencing vectors.The TYMV-mediated gene silencing efficiency was higher than the TRV-based VIGS system in radish.The expression level of RsPDS was significantly inhibited using VIGS in'NAU-067'radish leaves.The rootless seedlings of‘NAU-067'were infected with Agrobacterium rhizogenes using the 2300GN-Ubi-RsPDS-Cas9 vector with two target sequences.Nine adventitious roots were blue with GUs staining,and four of these adventitious roots were edited at target sequence 1 of the RsPDS gene as indicated by Sanger sequencing.Furthermore,albino lines were generated with A.tumefaciens-mediated transformation of radish cotyledons.Five base substitutions and three base deletions occurred at target sequence 2 in Line 1,and three base insertions and three base substitutions occurred at target sequence 1 in Line 2.This study shows that VIGS and CRISPR/Cas9 techniques can be employed to precisely verify the biological functions of genes in radish,which will facilitate the genetic improvement of vital horticultural traits in radish breeding programs.

关键词： Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS

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基于多维关系和用户聚类的智慧图书馆个性化图书推荐研究

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现代计算机 2023年第14期29卷 62-65,73页

作者：闫俊辉运城学院数学与信息技术学院运城044000

如何为用户提供感兴趣的个性化推荐图书商品向来都是智慧图书馆最核心的难题之一。因此,利用优质的推荐算法构建推荐系统就变得尤为重要。基于多维关系和用户聚类两个方面构建推荐算法,在充分考虑用户之间相关关系和图书商品之间相关关... 详细信息

如何为用户提供感兴趣的个性化推荐图书商品向来都是智慧图书馆最核心的难题之一。因此,利用优质的推荐算法构建推荐系统就变得尤为重要。基于多维关系和用户聚类两个方面构建推荐算法,在充分考虑用户之间相关关系和图书商品之间相关关系的基础上,不断更新“用户-图书商品”二维矩阵,使其数值更加合理和真实。随后使用k均值聚类方法聚拢高相关性用户。最后在类内选取目标用户实现个性化图书商品推荐。实验结果表明,该推荐算法能取得较高的评价指标F1值,即算法更加优质和有效。

关键词：智慧图书馆多维关系用户聚类个性化推荐系统

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