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Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish

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Journal of Integrative Agriculture 2024年第5期23卷 1557-1567页

作者： Jiali Ying Yan Wang Liang Xu Tiaojiao Qin Kai Xia Peng Zhang Yinbo ma Keyun Zhang Lun Wang junhui Dong Lianxue Fan Yuelin Zhu Liwang Liu National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization/Key Laboratory of Horticultural Crop Biology and Genetic Improvement(East China)of Ministry of Agriculture and Rural Affairs/College of Horticulture Nanjing Agricultural UniversityNanjing210095China College of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China

Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification in modern plant biotechnology.However,the investigation of gene silencing and editing in radish remains limited.In this study,a bleaching phenotype was generated through the knockdown of RsPDS using tobacco rattle virus(TRV)-and turnip yellow mosaic virus(TYMV)-mediated gene silencing vectors.The TYMV-mediated gene silencing efficiency was higher than the TRV-based VIGS system in radish.The expression level of RsPDS was significantly inhibited using VIGS in'NAU-067'radish leaves.The rootless seedlings of‘NAU-067'were infected with Agrobacterium rhizogenes using the 2300GN-Ubi-RsPDS-Cas9 vector with two target sequences.Nine adventitious roots were blue with GUs staining,and four of these adventitious roots were edited at target sequence 1 of the RsPDS gene as indicated by Sanger sequencing.Furthermore,albino lines were generated with A.tumefaciens-mediated transformation of radish cotyledons.Five base substitutions and three base deletions occurred at target sequence 2 in Line 1,and three base insertions and three base substitutions occurred at target sequence 1 in Line 2.This study shows that VIGS and CRISPR/Cas9 techniques can be employed to precisely verify the biological functions of genes in radish,which will facilitate the genetic improvement of vital horticultural traits in radish breeding programs.

关键词： Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS

聚二甲基硅氧烷封装石墨烯基柔性红外探测器的制备及其应用

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化学学报 2020年第2期78卷 161-169页

作者：赵雅婧谢亮马兰超贺军辉中国科学院理化技术研究所微纳材料与技术研究中心功能纳米材料实验室北京100190 北京石油化工学院材料科学与工程学院北京102617 中国科学院大学北京100049

采用热还原法在不同退火温度(从100到1200℃)下制备了一系列还原氧化石墨烯(r GO)薄膜,并通过X射线粉末衍射(XRD)、傅里叶变换红外光谱(FTIR)、拉曼光谱、四探针电阻仪和扫描电镜(SEM)对其进行了表征和分析.随后利用旋涂封装法制备了聚... 详细信息

采用热还原法在不同退火温度(从100到1200℃)下制备了一系列还原氧化石墨烯(r GO)薄膜,并通过X射线粉末衍射(XRD)、傅里叶变换红外光谱(FTIR)、拉曼光谱、四探针电阻仪和扫描电镜(SEM)对其进行了表征和分析.随后利用旋涂封装法制备了聚二甲基硅氧烷(PDMS)封装的石墨烯基柔性红外探测器(P-r GO-P),并成功用于红外激光、人体红外辐射、弯曲变化和压力探测.实验结果表明,采用不同温度还原的rGO薄膜封装得到的P-r GO-P柔性红外探测器在近红外(1064 nm)激光照射下均有响应,其中最高的光响应可达2.78 ma/W.此外, P-rGO-P柔性探测器对人体红外辐射和弯曲变化也具有快速、灵敏的响应,并且经过反复弯折仍能保持完整性与响应能力.

关键词：氧化还原石墨烯柔性光电转换红外探测器压力探测器

基于文本分类与语义识别的电力运营数据智能处理

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电子设计工程 2023年第9期31卷 104-108页

作者：马行星左军辉池俊国网新疆营销服务中心新疆乌鲁木齐830017

针对当前电力运营数据分析和利用不足的现状,提出一种基于文本分类与语义识别的电力运营数据智能处理算法。该算法采用剔除异常文本、分词以及去停顿词等操作进行电力运营文本清洗,再使用连续词袋(CBOW)模型实现电力运营文本的向量化表... 详细信息

针对当前电力运营数据分析和利用不足的现状,提出一种基于文本分类与语义识别的电力运营数据智能处理算法。该算法采用剔除异常文本、分词以及去停顿词等操作进行电力运营文本清洗,再使用连续词袋(CBOW)模型实现电力运营文本的向量化表示。同时利用Apriori算法挖掘电力运营文本向量与文本分类结果的关联规则,并基于小批量梯度下降(MBGD)的长短期记忆网络(LSTM)算法来获取电力运营文本分类的结果。通过对新疆电网某历史数据的仿真分析结果表明,文中所提算法相较于LSTM与Apriori-SVM算法,在电力运营文本分类上具有更高的准确率;且与传统梯度下降法相比,MBGD算法能够在保持较高准确率的同时大幅缩减模型的训练时间。

关键词：文本挖掘 Apriori 向量化表示长短期记忆网络

玉米-棉籽粕型饲粮添加过瘤胃赖氨酸和过瘤胃蛋氨酸对育肥湖羊生长性能、屠宰性能、器官指数和肉品质的影响

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动物营养学报 2023年第6期35卷 3812-3821页

作者：曹广吕刚刘伟刘保仓韩坤马军辉马学军新疆泰昆集团股份有限责任公司昌吉831100 新疆昌吉市二六工镇农业(畜牧业)发展服务中心昌吉831108

本试验旨在探究玉米-棉籽粕型饲粮添加过瘤胃赖氨酸(RPLys)和过瘤胃蛋氨酸(RPMet)对育肥湖羊生长性能、屠宰性能、器官指数和肉品质的影响。试验选用160头年龄(5月龄)和体重[(38.00±0.63) kg]相近的健康育肥期湖羊公羊,随机分为4个组,... 详细信息

本试验旨在探究玉米-棉籽粕型饲粮添加过瘤胃赖氨酸(RPLys)和过瘤胃蛋氨酸(RPMet)对育肥湖羊生长性能、屠宰性能、器官指数和肉品质的影响。试验选用160头年龄(5月龄)和体重[(38.00±0.63) kg]相近的健康育肥期湖羊公羊,随机分为4个组,每组40头羊。对照组饲喂基础饲粮,RPLys添加组在基础饲粮中添加3 g/kg RPLys,RPMet添加组在基础饲粮中添加2 g/kg RPMet,RPLys+RPMet添加组在基础饲粮中添加3 g/kg RPLys+2 g/kg RPMet。预试期7 d,正试期60 d。结果表明:1)与对照组相比,各过瘤胃氨基酸添加组育肥湖羊终末体重和平均日增重(ADG)显著提高(P<0.05)。2)与对照组相比,RPLys添加组和RPLys+RPMet添加组育肥湖羊胴体重、屠宰率、净肉重和净肉率均显著提高(P<0.05),RPMet添加组胴体重、净肉重和净肉率也显著提高(P<0.05)。3)RPLys添加组育肥湖羊肝脏相对重量显著高于其他各组(P<0.05),且RPMet添加组和RPLys+RPMet添加组肝脏相对重量显著高于对照组(P<0.05)。与对照组相比,各过瘤胃氨基酸添加组育肥湖羊肾周脂肪组织相对重量显著提高(P<0.05),RPLys添加组和RPLys+RPMet添加组尾脂组织相对重量显著提高(P<0.05)。4)与对照组相比,各过瘤胃氨基酸添加组育肥湖羊背最长肌45 min pH显著降低(P<0.05),RPMet添加组背最长肌蒸煮损失显著降低(P<0.05)。综上所述,在玉米-棉籽粕型饲粮中添加RPLys和RPMet可提高育肥湖羊生长性能和屠宰性能,提高肝脏、肾周脂肪和尾脂组织相对重量,改善肉品质,且以添加RPLys效果较佳。

关键词：过瘤胃赖氨酸过瘤胃蛋氨酸湖羊生长性能屠宰性能器官指数肉品质

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健康童鞋的繁荣先驱

中国卫生产业 2010年第3期7卷 93-95页

作者：马军辉《中国卫生产业》编辑部

健康越来成为人们日常生活追求的目标与此同时，很多企业也看到我们国内健康产业的发展空间。当下无论是童鞋企业2010年的订贷会主题。还是企业的品牌核心定位，都四处可见“健康”的口号.

关键词：健康产业童鞋制鞋工业发展现状

绩效考核纵横谈——对话北京同合益众医院管理咨询公司董事长梁庭斌

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中国卫生产业 2010年第3期7卷 76-79页

作者：马军辉《中国卫生产业》编辑部

公立医院改革错综复杂，内容之一的“绩效考核”,如何做到合情合理．更是莫衷一是。从事多年医院管理咨询工作并在绩效考核上锻造不少成功案例的梁庭斌．近日接受本刊专访畅论医院绩效考核.

关键词：绩效考核医院管理咨询公司董事长北京医院改革咨询工作

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国医大师讲养生

中国卫生产业 2010年第3期7卷 88-92页

作者：马军辉《中国卫生产业》编辑部

2010年，国家提出大力发展中医药产业。又逢春节临近，养生之首成为人们谈论的重点，究竟应该怎样养生，却不是每个人都能说清楚的。中医药能否养生，还是听听这位年逾八旬的老先生的见解吧！

关键词：养生国医大师中医药产业

Rational construction of high-active CO_(3)O_(4) electrocatalysts for oxygen evolution reaction

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Nano Research 2023年第1期16卷 624-633页

作者： Tianyun Zhang Shichao Zhao Chuanming Zhu Jing Shi Chao Su Jiawen Yang Meng Wang Jun Li junhui Li Pingle Liu Conghui Wang College of Chemical Engineering Xiangtan UniversityXiangtan 411105China State Key Laboratory of Coal Conversion Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China Analytical Instrumentation center Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China

Rational construction of high-efficiency electrocatalysts for oxygen evolution reaction(OER)is critical for renewable-energy technologies,but it is highly challenging to rationally regulate their surface structures to improve the OER performance.Herein,we proposed a“model-etching”strategy to investigate chemical etching of CO_(3)O_(4).The cubic CO_(3)O_(4)nanocrystals enclosed by well-defined facets are synthesized as model crystals,whose uniform surface structures allow us to study the etching mechanism at atomic level.Etching kinetics study together with DFT calculations discloses that{111}facets,the highly active facets for OER,serve as etch-stop facets in the etching reaction and H_(2)SO_(4)molecules play a special role in creating surface Co2^(+),the active center of OER.These results direct us to rationally optimize the surface structures of CO_(3)O_(4) to develop highly active OER electrocatalysts.The favorable performance of overpotential(η)and the Tafel slope decrease even to 268 mV@10 ma·cm−2 and 74 mV·dec−1,respectively.In general,our study shows that chemical etching of model crystals could help us rationally construct high-efficiency electrocatalysts.

关键词： cobalt oxide chemical etching high active facets oxygen evolution reaction surface structure

Mechanical and Galvano-chemistry Property Variation within Dissimilar Metal Weld between 1Cr18Ni9Ti and 1Cr13 Stainless Steel

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Journal of materials Science & Technology 2010年第5期26卷 477-480页

作者： Yongtao Zhao ,junhui Dong,Yonglin ma,Liping Zhao and Xiaobing Pei material and Metallurgy Engineering School,University of Science and Technology Inner Mongolia,Baotou 014010,China material and Metallurgy Engineering School University of Science and Technology Inner Mongolia Baotou 014010 China

The purpose of this study is to discuss the microstructure and properties variation of the weld metal in the dissimilar stainless steels during the argon tungsten-arc welding process.Because of the existence of different materials and chemistry variation within welds,properties,such as tensile and fracture properties and so on,are expected to show spatial variation.In the study,microstructures were observed by optical and electron microscopy.Good appearance and uniform structure and typical dendrite structure were observed in welding joint.The micro-hardness tester and electronic universal stretcher were used to measure the mechanical properties of the weldments and base metals.The heat affected zone（HAZ） near 1Cr13 hardness is up to peak value of 1150 HV,the strength of weldments is nearly same as austenite stainless steel.In addition,through seawater immersion test,polarization curves and AC impedance spectroscopy of the weldments and base metals are obtained.It was found that corrosion resistance at welding joint lies between 1C13 and 1Cr18Ni9Ti.

关键词： 1Cr13,1Cr18Ni9Ti Welding joint Mechanical and galvano-chemistry property