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Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

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Journal of Integrative Plant Biology 2010年第10期52卷 891-903页

作者： Yuchul Jung jungan park Yunjung Choi Jin-Gweon Yang Donggiun Kim Beom-Gi Kim Kyunghee Roh Dong-Hee Lee Chung-Kyoon Auh Sukchan Lee Department of Genetic Engineering Sungkyunkwan University Institute of Life Science and Technology Sungkyunkwan University National Academy of Agricultural Science Rural Development Administration Department of Life Science Ewha Womans University Department of Biological Sciences Mokpo National University

Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCI. The expression of At-pyrroline-5-carboxylate synthetase was not affected by NaCI stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCI. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCI. Mannitol induced L-proline accumulation more rapidly than NaCI or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.

关键词： Expression Analysis of Proline Metabolism-related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

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R ＆ D Activities for Power Semiconductor Devices in South Korea

R ＆ D Activities for Power Semiconductor Devices in South K...

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中国电工技术学会电力电子学会第八届学术年会

作者： Eun-Dong Kim Pill-Hwan park Power Semiconductor Research group Korea Electrotechnology Research Institute P. O. Box 20 Changwon 641-120 Gyungnam Republic of Korea Machinery and Electronics Technology Devision Ministry of Science and Technology 1st Jungan-dong Kwacheun 427-715 Kyungki Republic of Korea

＜正＞ Nation-wide research and development activities for power semiconductor devices in South Korea have been promoted by sponsorship of the Government, the Ministry of Science and Technology, since 1997. The activities have covered a wide range of devices including insulated gate bipolar transistors and their intelligent modules. The sponsorship is expected to advance the realization of ’Power System on Chip’ through intelligent power integrated circuits. Our efforts have given very positive effects for the domestic companies to penetrate the conservative power semiconductor market with new devices and to expand their market shares. Help of national research institutes and universities has certainly encouraged for emerging companies to introduce their products to the market.

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