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Microplastics in sediment of the Three Gorges Reservoir:abundance and characteristics under different environmental conditions

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Journal of Oceanology and Limnology 2024年第1期42卷 101-112页

作者： Wang LI Bo ZU Yiwei LIU juncheng guo Jiawen LI College of River and Ocean Engineering Chongqing Jiaotong UniversityChongqing 400074China Chongqing Research Academy of Ecology and Environmental Sciences Chongqing 401147China

Freshwater microplastic pollution is an urgent issue of global concern,and research on the distribution in reservoirs is *** investigated the microplastic pollution levels in wet sediments collected from the Three Gorges Reservoir,the largest reservoir of *** show that microplastics were ubiquitous in the sediments of the Three Gorges Reservoir,and their abundance ranged from 59 to 276 pp/kg(plastic particles per kg,dry weight).Economic development and total population were important factors affecting the spatial heterogeneity of microplastic abundance,and the contribution of large cities along the reservoir to microplastic pollution should be paid with more *** microplastics were the most abundant type of microplastic particles in reservoir sediments,whereas polystyrene,polypropylene,and polyamide were the main types of *** apparent spatial heterogeneity in morphology and color of microplastics is attributed to different anthropogenic or landbased pollution ***,the accumulation of microplastics near the inlet of tributaries reflects the role of potential contributors of *** importantly,multiple bisphenols(BPs)and heavy metals detected at the microplastic surfaces indicate that microplastics can act as carriers of these pollutants in the environment in the same way as sediments did,which may alter the environmental fate and toxicity of these ***,we conclude that the Three Gorges Reservoir had been contaminated with microplastics,which posed a stress risk for organisms who ingest them along with their associated pollutants(BPs,heavy metals).

关键词： microplastics Three Gorges Reservoir sediment bisphenol heavy metal

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Advances in the Treatment of Advanced Non-Small Cell Lung Cancer

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Advances in Lung Cancer 2020年第2期9卷 30-40页

作者： Xianshan Chen juncheng guo Min guo Hainan General Hospital Haikou China Central South University Xiangya School of Medical Affiliated Haikou Hospital Haikou China

In recent years, the incidence of lung adenocarcinoma has been increasing, and now it has become the largest type of non-small cell lung cancer (NSCLC). Currently, treatment of advanced NSCLC consists of several modalities: systemic chemotherapy, local radiation therapy, and targeted therapy (including most recently immunotherapy). In the past decade, the discovery of new molecular subtypes, the search for tumor driver gene mutations, the development of targeted molecular targeted drugs, or targeted therapy to suppress tumor angiogenesis and regulate tumor immune response have been the main directions of NSCLC research and clinical diagnosis and treatment. At present, platinum-based chemotherapy is widely used in NSCLC patients clinically. Platinum-based chemotherapy drugs can effectively prolong the survival time of patients and improve their quality of life, but the incidence of adverse reactions is still high. Therefore, it is necessary to find a drug that can improve the efficacy of patients and reduce the adverse reactions of platinum chemotherapy drugs to NSCLC patients.

关键词： Non-Small Cell Lung Cancer Drugs Targeted Chemotherapy

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BDNF Gene Frequency Distribution in Li and Han Ethnic Groups in Hainan Province and Its Comparison with Other Countries and Regions

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Journal of Behavioral and Brain Science 2020年第12期10卷 531-536页

作者： Min guo juncheng guo Yunsuo Gao Xiaodan Wang Hainan General Hospital Haikou China Central South University Xiangya School of Medical Affiliated Haikou Hospital Haikou China School of Public Health Hainan Medical University Haikou China

Objective: To explore the distribution characteristics of BDNF gene frequency in Li and Han nationalities in Hainan province. Methods: In June 2018-2019 and march to the people’s hospital of Hainan province health volunteers, 152 cases (Li 80, Han, 72), the application of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect the polymorphism of BDNF gene (rs6265), and compared with other countries and regions of ethnic comparison between BDNF gene frequency distribution. Results: No statistical difference was found in rs6265 genotype distribution of BDNF gene in Li and Han ethnic groups (P = 0.3358, P = 0.0892, P = 0.1549). Comparison of BDNF allele frequency between Li and other ethnic groups showed statistically significant differences with other ethnic groups in China and Europe (P = 0.0384, P = 0.0000), but not statistically significant differences with Japan (P = 0.1164). Conclusion: BDNF gene (rs6265) has polymorphism in both Li and Han ethnic groups in Hainan Province. Compared with ethnic groups in other countries and regions, the polymorphism distribution is ethnically different.

关键词： BDNF Gene The PCR-RFLP Alleles Genotype

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Research on HOXA11-AS in Malignant Tumors

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Journal of Cancer Therapy 2021年第1期12卷 19-30页

作者： juncheng guo Jinfang Zheng Yijun Yang Affiliated Haikou Hospital of Central South University Xiangya School of Medicine Haikou China Liver and Gallbladder Surgery Hainan General Hospital Haikou China

Long-chain non-coding RNA HOXA11 antisense RNA (HOXA11-AS) is a kind of lncRNA discovered in recent years. Long-chain non-coding RNA (LncRNA) is an important regul

关键词： Long Non-Coding RNA HOXA11-AS Malignant Tumor

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MIR-448 Regulates MAGEA6/AMPK Signaling Pathway in Hepatocellular Carcinoma Tumor Stem Cells

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Journal of Cancer Therapy 2023年第4期14卷 182-201页

作者： Changliang Jiao Jinfang Zheng juncheng guo Hainan Vocational University of Science and Technology Haikou China Hainan Provincial People’s Hospital Haikou China Haikou Hospital Xiangya School of Medicine Central South University Haikou China

Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway in the biological study of hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using the database, the hepatocellular carcinoma related expression chips were obtained and the regulatory mirnas of candidate genes were predicted, and the predicted results were analyzed. The effects of miR-448 and MAGEA6 on the pellet formation rate and clone formation rate of hepatocellular carcinoma stem cells were detected by immunofluorescence identification of stem cell markers and light microscope counting method. The effects of miR-448 and MAGEA6 on migration and invasion of hepatocellular carcinoma stem cells were detected by scratch and Transwell assay. Dual luciferase reporter assay to verify whether miR-448 targets MAGEA6. The expression and influence of miR-448 on MAGEA6 and AMPK pathway were detected by qRT-PCR and Western blot. Results: It was found that miR-448 may directly regulate the expression of MAGEA6. Overexpression of miR-448 inhibited the characteristics, proliferation, migration, and invasion of hepatocellular carcinoma stem cells in vitro, as well as the ability of xenograft tumor formation in vivo. However, inhibition of miR-448 showed opposite results. In addition, miR-448 directly targets MAGEA6 and regulates AMPK signaling. Silencing MAGEA6 and adding AMPK activator further verified that miR-448 activated AMPK signaling pathway by targeting MAGEA6, thus affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. Conclusions: Our results reveal that miR-448 activates AMPK signaling pathway by targeting MAGEA6, thereby affecting characteristics, proliferation, migration and invasion of hepatoma stem cells. It is suggested that overexpression of miR-448 may be a new therapeutic strategy for hepatocellular carcinoma.

关键词： mir-448 MAGEA6 AMPK Signaling Pathway Liver Cancer Tumor Stem Cells

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Comorbidity between Neurotic Depression and Post-Traumatic Stress Disorder (PTSD)

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World Journal of Neuroscience 2020年第3期10卷 135-140页

作者： Huang Tao Chunru Wang juncheng guo Min guo Hainan General Hospital Haikou China People’s Hospital of Qionghai City Qionghai China Central South University Xiangya School of Medical Affiliated Haikou Hospital Haikou China

Comorbidity with PTSD has been studied at home and abroad in follow-up studies following major traumatic events, indexing patients diagnosed with PTSD and investigating rates of comorbidity and other psychiatric disorders. In practical clinical practice, the majority of patients with PTSD are diagnosed with neurosis and depression, but the clinical manifestations and treatment of some of these patients are more complicated and difficult, and it is clinically found that these patients may have comorbidities and other mental disorders. This study in hospitalized patients with neurosis and depression as the main research object, through the investigation found that some patients (36.4%) of comorbid PTSD, just, this part of the patients reported by a traumatic event is not CCMD-3 defined in the “unusual” traumatic event. Therefore, the results of this study suggest that attention should be paid to the collection of patients’ medical history, especially the inquiry of traumatic experiences. This study is only a preliminary study with a small sample size, and the conclusions obtained need to be further confirmed in future studies.

关键词： Neurosis Depression Comorbidities Post-Traumatic Stress Disorder

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Study on the Relationship between 5-HttLPR Gene and BDNF Gene Polymorphism and Post-Traumatic Stress Disorder in Li and Han Nationality of Hainan Province

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Health 2022年第1期14卷 158-175页

作者： Haiyan Lin juncheng guo Min guo Xiangling Jiang The Fourth People’s Hospital of Haikou Haikou China Hainan Provincial People’s Hospital Haikou China

Objective: To investigate the correlation between 5-HTTLPR (5-and serotonin transporter linked polymer region) gene polymorphism and BDNF (brain derived neural factor) gene polymorphism and PTSD (post traumatic stress disorders) in Li and Han nationalities in Hainan Province. Methods: 167 Hainan Li PTSD patients, 141 Hainan Han PTSD patients and 158 healthy volunteers (control group) were investigated by ETI, caps, Toh, WCST, TMT and WAIS-RC. The polymorphisms of rs6265 locus of 5-HTTLPR and BDNF genes were detected by PCR (polymerase chain reaction) and page (polycylamide gel electrophoresis), and the correlation with PTSD was analyzed. Logistic regression analysis was used to analyze the influencing factors of PTSD. Results: The ETI score, total PTSD score and TMT time of Li PTSD patients were significantly higher than those of Han PTSD patients (P 0.05). SS genotype of 5-HTTLPR and (GA + AA) genotype of rs6265 locus may increase the risk of PTSD in Hainan Han population. AA and GA + AA genotypes at rs6265 locus may increase the risk of PTSD in Li population (P < 0.05). Among Li PTSD patients, the ETI score, PTSD total score, TMT time, Toh planning time and execution time of AA genotype at rs6265 locus were significantly higher than those of GG genotype;the total scores of comprehension and operation IQ, and Toh in WAIS-RC were significantly lower than those in GG genotype (P < 0.05). Among Han PTSD patients, the ETI score, PTSD total score and TMT time of SS genotype of 5-HTTLPR were significantly high

关键词： 5-HTTLPR BDNF Gene Polymorphism Post-Traumatic Stress Disorder Li Nationality Han Nationality Frequency Distribution

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A Study on the Association between Patients with Post-Traumatic Stress Disorder in Li and Han Ethnic Groups in Hainan Province and DNA Methylation of Brain-Derived Neurotrophic Factor Genes

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Journal of Behavioral and Brain Science 2020年第10期10卷 400-409页

作者： Xiaodan Wang Yunsuo Gao Min guo juncheng guo School of Public Health Hainan Medical University Haikou China Hainan General Hospital Haikou China Central South University Xiangya School of Medical Affiliated Haikou Hospital Haikou China

Objective: To explore the pathogenesis of PTSD in the brain-derived neurotrophic factor (BDNF) gene methylation of patients with posttraumatic stress disorder (Posttraumatic Stress Disorder, PTSD) in Hainan Province, the relationship between the influence of BDNF gene methylation and the influence of PTSD. Methods: A case-control study method was adopted, strictly in accordance with DSM-IV and PTSD diagnosis, and 150 Li PTSD patients matched with gender and age of 300 Han PTSD patients were selected as the research objects. The peripheral venous whole blood of the subjects was drawn, genomic DNA was extracted, modified with bisulfite, and directly sequenced to quantitatively detect the methylation status of the CpG island in the promoter region of brain-derived neurotrophic factor (BDNF). Results: The results showed that the methylation levels of CPGl, CPG2, CPG3, CPG4, CPG5, CPG6, CPG7, CPG9, CPGl2, CPGl3, CPGl4, CPGl5, CPGl6, CPGl7, and CPGl8 in THE BDNF promoter were significantly different between the HAN PTSD group and the Li PTSD group (P Conclusion: It is suggested that CPG methylation in the promoter region of BDNF gene is closely related to patients with PTSD. There is a statistical difference in the level of CpG methylation in the promoter region of BDNF gene in PTSD between Li and Han ethnic groups in Hainan Province. CpG methylation in the promoter region of BDNF gene may be used as a biomarker for the diagnosis of PTSD.

关键词： Ethnicity Brain-Derived Neurotrophic Factor DNA Methylation Post-Traumatic Stress Disorder Epigenetics

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A Review of Studies on Ethnic Differences of Brain-Derived Neurotrophic Factor Genes in Patients with Post-Traumatic Stress Disorder

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World Journal of Neuroscience 2019年第4期9卷 229-242页

作者： Liumei Luo juncheng guo Xiang Li Min guo Ping Huang Hainan General Hospital Sanya China Haikou Hospital Affiliated to Xiangya School of Medicine Central South University Haikou China The Third People’s Hospital of Hubei Province Wuhan China University of South China Hengyang China

In the regulation of human nervous system, cognitive function and other genes, epigenetics changes the expression of genes after being influenced by the external environment. DNA methylation levels are also different in different ethnic groups, and a large number of studies have shown that post-traumatic stress disorder (PTSD) has a certain genetic predisposition. Through the national differences of PTSD brain-derived neurotrophic factor genes, it not only provides new research directions for the pathogenesis and treatment of 5-HT-related mental diseases, but also provides information and new genetic indicators for forensic personal identification, paternity testing and assessment of mental status. A review studies on the national differences of brain-derived neurotrophic factor genes in patients with post-traumatic stress disorder.

关键词： Ethnicity Post-Traumatic Stress Disorder Brain-Derived Neurotrophic Factor Gene Methylation

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Correlation between Expression Differences of Epithelial-Mesenchymal Transition (EMT) in Cholangiocarcinoma Tissue

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International Journal of Clinical Medicine 2021年第8期12卷 328-341页

作者： Qi Liu Xiang Rao Haixiang Cai juncheng guo The Fourth People’s Hospital of Haikou Haikou China Hainan General Hospital Haikou China

Background: By studying the expression of epithelial-mesenchymal transition regulators in cholangiocarcinoma and intrahepatic duct stones, the correlation between the expression of epithelial-mesenchymal transition regulators and cholangiocarcinoma was revealed. Objective: The objective is to investigate the correlation between the expression of epithelial-mesenchymal transition (EC) regulatory factors and cholangiocarcinoma in patients with intrahepatic duct stones and cholangiocarcinoma, to investigate the relationship between clinicopathological features and prognosis, and to observe the expression of molecular markers of epithelial-mesenchymal transition (EMT) in intrahepatic duct stones and bile duct carcinoma. Methods: Twenty cases of primary cholangiocarcinoma, 20 cases of intrahepatic cholangiolithiasis complicated with cholangiocarcinoma, and 20 cases of intrahepatic cholangiolithiasis specimens were collected from the Fourth People’s Hospital and the friendly medical unit of Haikou. Immunohistochemistry was used to detect the expression differences of EMT-related molecular markers Twisit1, Twisit2, E-cadherin, N-cadherin, and Vimentin in paraffin sections of normal intrahepatic bile duct tissues and patients with intrahepatic duct stones and cholangiocarcinoma. Results: Immunohistochemical staining revealed epithelial-mesenchymal transition (EMT) in intrahepatic cholangiocarcinoma tissue, intrahepatic cholangiolithiasis with cholangiocarcinoma, intrahepatic cholangiolithiasis with normal intrahepatic cholangiolithiasis, such as Sit1, Twisit2, E-cadherin, N-cadherin, and Vimentin proteins were different. The expression of E-cadherin was decreased in cholangiocarcinoma tissue and intrahepatic cholangiolithiocarcinoma combined with cholangiocarcinoma (P < 0.05), while the expression of N-cadherin and Vimentin was up-regulated (P < 0.05). The expression of Twisit1 and Twisit2 had no difference

关键词： Intrahepatic Bile Duct Stones Hepatobiliary Cell Carcinoma Organiza-tions EMT. Immunohistochemical

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