检索结果-南通市图书馆

维普期刊数据库博看期刊

SPORTS1.0: A Tool for Annotating and Profiling Non-coding RNAs Optimized for rRNA-and tRNA-derived Small RNAs

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2018年第2期16卷 144-151页

作者： junchao shi Eun-A Ko Kenton M.Sanders Qi Chen Tong Zhou Department of Physiology and Cell Biology University of Nevada Reno School of Medicine

High-throughput RNAoseq has revolutionized the process of small RNA （sRNA） discovery, leading to a rapid expansion of sRNA categories. In addition to the previously wellcharacterized sRNAs such as microRNAs （miRNAs）, piwi-interacting RNAs （piRNAs）, and small nucleolar RNA （snoRNAs）, recent emerging studies have spotlighted on tRNA-derived sRNAs （tsRNAs） and rRNA-derived sRNAs （rsRNAs） as new categories of sRNAs that bear versatile functions. Since existing software and pipelines for sRNA annotation are mostly focused on analyzing miRNAs or piRNAs, here we developed the sRNA annotation pipeline _optimized for rRNA- and tRNA-derived s_RNAs （SPORTS 1 .0）. SPORTS1.0 is optimized for analyzing tsRNAs and rsRNAs from sRNA-seq data, in addition to its capacity to annotate canonical sRNAs such as miRNAs and piRNAs. Moreover, SPORTS1.0 can predict potential RNA modification sites based on nucleotide mismatches within sRNAs. SPORTS1.0 is precompiled to annotate sRNAs for a wide range of 68 species across bacteria, yeast, plant, and animal kingdoms, while additional species for analyses could be readily expanded upon end users＇ input. For demonstration, by analyzing sRNA datasets using SPORTS1.0, we reveal that distinct signatures are present in tsRNAs and rsRNAs from different mouse cell types. We also find that compared to other sRNA species, tsRNAs bear the highest mismatch rate, which is consistent with their highly modified nature. SPORTS1.0 is an opensource software and can be publically accessed at https：//***/junchaoshi/sports1.0.

关键词： Small RNA RNA-seq data analysistsRNA rsRNA Annotation pipeline

Evaluation and Hydrological Application of CMADS Reanalysis Precipitation Data against Four Satellite Precipitation Products in the Upper Huaihe River Basin, China

在线全文

学校读者我要写书评

暂无评论

Journal of Meteorological Research 2020年第5期34卷 1096-1113页

作者： Shanhu JIANG Ruolan LIU Liliang REN Menghao WANG junchao shi Feng ZHONG Zheng DUAN State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai UniversityNanjing 210098China Hydrology and Water Resources Monitoring and Forecasting Center Ministry of Water ResourcesBeijing 100053China Department of Physical Geography and Ecosystem Science L und UniversityLund S-22362Sweden

Satellite-and reanalysis-based precipitation products are important data source for precipitation, particularly in areas with a sparse gauge network. Here, five open-access precipitation products, including the newly released China Meteorological Assimilation Driving Datasets for the Soil and Water Assessment Tool(SWAT) model(CMADS)reanalysis dataset and four widely used bias-adjusted satellite precipitation products [SPPs;i.e., Tropical Rainfall Measuring Mission(TRMM) Multisatellite Precipitation Analysis 3B42 Version 7(TMPA 3B42V7), Climate Prediction Center(CPC) morphing technique satellite–gauge blended product(CMORPH-BLD), Climate Hazards Group Infrared Precipitation with Station Data(CHIRPS), and Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks–Climate Data Record(PERSIANN-CDR)], were assessed. These products were first compared with the gauge observed data collected for the upper Huaihe River basin, and then were used as forcing data for streamflow simulation by the Xin’anjiang(XAJ) hydrological model under two scenarios with different calibration procedures. The performance of CMADS precipitation product for the Chinese mainland was also assessed. The results show that:(1) for the statistical assessment, CMADS and CMORPH-BLD perform the best, followed by TMPA 3B42V7, CHIRPS, and PERSIANN-CDR, among which the correlation coefficient(CC) and rootmean-square error(RMSE) values of CMADS are optimal, although it exhibits certain significant negative relative bias(BIAS;-22.72%);(2) CMORPH-BLD performs the best in capturing and detecting rainfall events, while CMADS tends to underestimate heavy and torrential precipitation;(3) for streamflow simulation, the performance of using CMADS as input is very good, with the highest Nash–Sutcliffe efficiency(NSE) values(0.85 and 0.75 for calibration period and validation period, respectively);and(4) CMADS exhibits high accuracy in eastern China while with significant negative BIAS,

关键词： reanalysis precipitation data China Meteorological Assimilation Driving Datasets for the Soil and Water Assessment Tool(SWAT)model(CMADS) satellite precipitation hydrological evaluation Xin’anjiang(XAJ)hydrological model

3例犬炎性乳腺癌的病理学诊断和组织学分级评价

在线全文

学校读者我要写书评

暂无评论

中国兽医杂志 2024年第1期60卷 15-20页

作者：高蕊石俊超张竞贾茜涵马莹贺文琦陆慧君吉林大学动物医学学院吉林长春130062

为了对3例临床疑似犬炎性乳腺癌的病例进行确诊,本试验采用苏木精-伊红(H.E.)染色对送检肿物进行组织病理学检查,应用全角蛋白(Pan ck)抗体、角蛋白8(CK8)抗体和血管性血友病因子(VWF)抗体进行免疫组织化学染色以判断免疫表型。结果显... 详细信息

为了对3例临床疑似犬炎性乳腺癌的病例进行确诊,本试验采用苏木精-伊红(H.E.)染色对送检肿物进行组织病理学检查,应用全角蛋白(Pan ck)抗体、角蛋白8(CK8)抗体和血管性血友病因子(VWF)抗体进行免疫组织化学染色以判断免疫表型。结果显示,3例患犬肿瘤内部形成管状癌、致密癌、筛状癌和粉刺性癌,并伴有淋巴管扩张、肿瘤栓子浸润淋巴管等现象,符合犬炎性乳腺癌的特征;免疫组织化学染色结果证实,肿瘤为腺上皮起源,且淋巴管、血管内存在大量肿瘤栓子。通过病理学分析结合临床表现,3例病例均确诊为犬炎性乳腺癌。根据Pena分级标准,将病例1判定为Ⅲ级炎性乳腺癌,病例2和3判定为Ⅱ级炎性乳腺癌。本试验结果不仅可为犬炎性乳腺癌的病理诊断提供参考,而且为犬炎性乳腺癌临床病例治疗方案的制定和预后评估提供理论依据。

关键词：犬炎性乳腺癌病理组织学免疫组织化学

Denoising Autoencoder, A Deep Learning Algorithm, Aids the Identification of A Novel Molecular Signature of Lung Adenocarcinoma

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2020年第4期18卷 468-480页

作者： Jun Wang Xueying Xie junchao shi Wenjun He Qi Chen Liang Chen Wanjun Gu Tong Zhou Department of Thoracic Surgery Jiangsu Province People’s Hospital and the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China State Key Laboratory of Bioelectronics School of Biological Sciences and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Physiology and Cell Biology University of NevadaReno School of MedicineRenoNV 89557USA State Key Lab of Respiratory Disease Guangzhou Medical UniversityGuangzhou 510000China

Precise biomarker development is a key step in disease management. However, most of the published biomarkers were derived from a relatively small number of samples with supervised approaches. Recent advances in unsupervised machine learning promise to leverage very large datasets for making better predictions of disease biomarkers. Denoising autoencoder(DA) is one of the unsupervised deep learning algorithms, which is a stochastic version of autoencoder techniques. The principle of DA is to force the hidden layer of autoencoder to capture more robust features by reconstructing a clean input from a corrupted one. Here, a DA model was applied to analyze integrated transcriptomic data from 13 published lung cancer studies, which consisted of 1916 human lung tissue samples. Using DA, we discovered a molecular signature composed of multiple genes for lung adenocarcinoma(ADC). In independent validation cohorts, the proposed molecular signature is proved to be an effective classifier for lung cancer histological subtypes. Also, this signature successfully predicts clinical outcome in lung ADC, which is independent of traditional prognostic factors. More importantly, this signature exhibits a superior prognostic power compared with the other published prognostic genes. Our study suggests that unsupervised learning is helpful for biomarker development in the era of precision medicine.

关键词： Denoising autoencoder Unsupervised learning Lung cancer Prognosis Molecular signature

通调督脉针刺法配合认知康复训练治疗脑卒中后认知障碍疗效观察

在线全文

学校读者我要写书评

暂无评论

实用中医药杂志 2024年第7期40卷 1413-1415页

作者：陈悦琦汪婷婷史骏超胥琨王雯崔晓上海市长宁区天山中医医院上海200051

目的:观察通调督脉针刺法联合认知康复训练治疗脑卒中后认知障碍(PSCI)的疗效。方法:72例随机分为两组各36例,观察组予针刺联合认知康复训练,对照组予认知康复训练。结果:两组治疗后MoCA评分、MBI评分、FMA评分均升高(P<0.05),且观察组... 详细信息

目的:观察通调督脉针刺法联合认知康复训练治疗脑卒中后认知障碍(PSCI)的疗效。方法:72例随机分为两组各36例,观察组予针刺联合认知康复训练,对照组予认知康复训练。结果:两组治疗后MoCA评分、MBI评分、FMA评分均升高(P<0.05),且观察组高于对照组(P<0.05)。结论:通调督脉针刺法联合认知康复训练能有效改善PSCI患者认知功能、肢体功能及日常生活自理能力。

关键词：脑卒中认知障碍针刺康复训练

GRACE observed mass loss in the middle and lower Yangtze basin

在线全文

学校读者我要写书评

暂无评论

Geodesy and Geodynamics 2019年第2期10卷 157-162页

作者： Jiangjun Ran Natthachet Tangdamrongsub junchao shi Changqing Wang Lihui Wang Xiaoyun Wan Institute of Geodesy and Geophysics Chinese Academy of Sciences School of Engineering University of Newcastle Information Center Ministry of Water Resources Qian Xuesen Laboratory of Space Technology

Anthropogenic architectures have a significant impact to the environment. The Three Gorges Dam(TGD),as the largest dam in the world, is a typical example, and has influenced the mass anomalies transported by the flow downstream in the Yangtze River since June 2003. However, the evidence of TGD influence on downstream mass transportation is not documented. In this study, we analyze the monthly gravity solutions from GRACE to investigate the downstream mass variations of Yangtze River. From our results,the considerable mass loss is detected in the downstream of TGD. By comparing our estimations with the in situ water level data of TGD, we find that the mass variations derived from GRACE at Datong station decreases shortly after the impoundment of TGD. This confirms a strong connection between ***, by comparing with the in situ sediment load and river discharge at Yichang and Datong gauging stations, we find that the sediment load data shows a similar mass loss signature while the river discharge of both stations has stay at a relative stable level. This indicates that further study is still needed to understand the mechanism better.

关键词： GRACE Mass loss The Yangtze River basin Sediment load River discharge

在线全文

学校读者我要写书评

暂无评论

基于深度学习的轨条砦障碍毁伤等级识别研究

火箭军工程大学学报

火箭军工程大学学报 2024年第4期38卷 34-39+46页

作者：熊建武万文乾姜波朱会杰史俊超陆军研究院五所

针对传统毁伤评估方法需要大量试验数据修正毁伤评估模型，且需要对炸点位置进行测量并结合弹目交汇条件才能计算毁伤效果的问题，提出了一种基于图像识别的端到端轨条砦障碍毁伤等级识别方法，进而快速对毁伤后轨条砦进行毁伤等级判定... 详细信息

针对传统毁伤评估方法需要大量试验数据修正毁伤评估模型，且需要对炸点位置进行测量并结合弹目交汇条件才能计算毁伤效果的问题，提出了一种基于图像识别的端到端轨条砦障碍毁伤等级识别方法，进而快速对毁伤后轨条砦进行毁伤等级判定。该方案基于改进的YOLO5算法实现轨条砦图像检测、毁伤特征识别和毁伤等级评估。首先，由专家对轨条砦数据集进行标注，利用改进的马赛克拼接提高小目标检测能力；然后，使用卷积块注意力模块代替部分卷积模块，提升模型整体性能；最后，针对轨条砦的尺寸特点优化检测端结构，移除大目标尺度特征以减少推理计算量。实验结果表明：该方法可以在保证检测实时性的同时，毁伤评估精度达到98%。

关键词：轨条砦神经网络目标检测毁伤评估

Development of mouse preimplantation embryos in space

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2020年第9期7卷 1437-1446页

作者： Xiaohua Lei Yujing Cao Baohua Ma Yunfang Zhang Lina Ning Jingjing Qian Liwen Zhang Yongcun Qu Tao Zhang Dehong Li Qi Chen junchao shi Xudong Zhang Chiyuan Ma Ying Zhang Enkui Duan State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of Sciences University of Chinese Academy of Sciences Shanghai Institute of Technical Physics Chinese Academy of Sciences Division of Ionizing Radiation National Institute of Metrology College of Veterinary Medicine Northwest A&F University/Key Laboratory of Animal BiotechnologyMinistry of Agriculture

The development of life beyond planet Earth is a long-standing quest of the human race, but whether normal mammalian embryonic development can occur in space is still unclear. Here, we show unequivocally that preimplantation mouse embryos can develop in space, but the rate of blastocyst formation and blastocyst quality are compromised. Additionally, the cells in the embryo contain severe DNA damage, while the genome of the blastocysts developed in space is globally hypomethylated with a unique set of differentially methylated regions. The developmental defects, DNA damage and epigenetic abnormalities can be largely mimicked by the treatment with ground-based low-dose radiation. However,the exposure to simulated microgravity alone does not cause major disruptions of embryonic development,indicating that radiation is the main cause for the developmental defects. This work advances the understanding of embryonic development in space and reveals long-term extreme low-dose radiation as a hazardous factor for mammalian reproduction.

关键词： space-flight radiation microgravity preimplantation development DNA damage DNA methylation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

《儒林外史》的水路空间叙事与江南书写

明清小说研究 2023年第1期 143-159页

作者：史俊超南京大学文学院江苏第二师范学院文学院

作为与《红楼梦》双峰并峙的清代章回小说,《儒林外史》不但具有出色的讽刺性,而且它的写实风格也颇具特色。江南地区丰富的水网以及运河作为枢纽沟通其中,为小说提供了广阔的叙事空间。水路交通的载体航船成为叙事空间的重要组成部分,... 详细信息

作为与《红楼梦》双峰并峙的清代章回小说,《儒林外史》不但具有出色的讽刺性,而且它的写实风格也颇具特色。江南地区丰富的水网以及运河作为枢纽沟通其中,为小说提供了广阔的叙事空间。水路交通的载体航船成为叙事空间的重要组成部分,许多情节的转换,故事的发生都与之相关。从小说中我们可以看出江南地区山水之间的互动,典型的江南书写也体现了吴敬梓的人生理想和全书主题。

关键词：《儒林外史》水路航船叙事空间江南