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Diversity, structure and function of the coiled-coil domains of plant NLR immune receptors

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Journal of Integrative Plant Biology 2021年第2期63卷 283-296页

作者： junzhu Wang Meng Han Yule Liu MOE Key Laboratory of Bioinformatics Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for Life Sciences Beijing 100084China

Plant nucleotide-binding, leucine-rich repeat receptors(NLRs) perceive pathogen avirulence effectors and activate defense ***-binding, leucine-rich repeat receptors are classified into coiled-coil(CC)-containing and Toll/interleukin-1 receptor(TIR)-containing NLRs. Recent advances suggest that NLR CC domains often function in signaling activation, especially for induction of cell death. In this review, we outline our current understanding of NLR CC domains, including their diversity/classification and structure, their roles in cell death induction, disease resistance, and interaction with other proteins. Furthermore, we provide possible directions for future work.

关键词： cell death classification coiled-coil domain disease resistance NLR structure

Michael Additiion of Alkyl Amine to α,β-Unsaturated Carbonyl Compounds Catalyzed by KF/Al_2O_3

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Chinese Chemical Letters 2004年第8期15卷 883-884页

作者： DanBiTIAN junzhu JieFangZHU YuanXiangSHI JinTangWANG SchoolofScience NanjingUniversityofTechnologyNanjing210009

The β-aminocarboxylic acid derivatives were synthesized by Michael addition reaction of alkyl amine and α,β-unsaturated carbonyl compounds in the presence of potassium fluoride on alumina.

关键词： Michael addition,β-aminocarboxylic acid derivatives KF/Al2O3.

Physical stimuli-responsive cell-free protein synthesis

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Synthetic and Systems Biotechnology 2020年第4期5卷 363-368页

作者： junzhu Yang Yuan Lu Key Laboratory of Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijingChina

Cell-free protein synthesis has been developed as a critical platform in synthetic *** the cell-based synthesis system,cell-free system activates transcriptional and translational mechanisms in vitro,and can control protein synthesis by artificially adding components or ***,the control method puts forward higher requirements in terms of accurate and non-toxic control,which cannot be achieved by chemical *** cell-free system,physical signal is a kind of ideal spatiotemporal control approach to replace chemical substances,realizing high accuracy with little side *** we review the methods of using physical signals to control gene expression in cell-free systems,including studies based on light,temperature,electric field,and magnetic *** transfer of these switches into cell-free system further expands the flexibility and controllability of the system,thus further expanding the application capability of cell-free ***,existing problems such as signal source and signal transmission are discussed,and future applications in pharmaceutical production,delivery and industrial production are further looked into.

关键词： Cell-free system Physical stimuli Spatiotemporal control Synthetic biology

Organ failure associated with severe acute pancreatitis

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World Journal of Gastroenterology 2003年第11期9卷 2570-2573页

作者： Ai-junzhu Jing-SenShi Xue-JunSun DepartmentofGeneralsurgery TheFirstHospitalofXi'anJiaotongUniversityXi’an710061ShaanxiProvinceChina DepartmentofGeneralsurgery TheFirstHospitalofXi'anjiaotongUniversityChinaShanxiProvinceXi’an710061

AIM: To investigate the relationship between severe acute pancreatitis (SAP) and organ ***: Clinical data of 74 cases of SAP from Jan. 1993 to Dec. 2002 were retrospectively reviewed, and the relationship between organ failure and age, gender, etiology,extent of necrosis, infection of necrosis and mortality was ***: A total of 47 patients (63.5 %) showed organ failure, 20 patients (27.0 %) multiple organ failure, whereas 27 patients (36.5 %) with dysfunction of a single organ system. Pulmonary failure was the most common organ dysfunction (23.0 %) among single organ failures. There were no significant differences in age, gender and gallstone pancreatitis among patients with or without organ failure (P>0.05). The incidence of organ failure in infected necrosis was not higher compared with sterile necrosis, and patients with increased amount of necrosis did not have an increased prevalence of organ failure (P>0.05). Patients with organ failure had a higher mortality rate compared with those without organ failure (P<0.05). The death of SAP was associated with multiple organ failure (P<0.005), pulmonary failure (P<0.005), cardiovascular dysfunction (P<0.05) and gastrointestinal dysfunction (P<0.05).CONCLUSION: Organ failure is common in patients with SAP, and patients with multiple organ failure and pulmonary failure have a higher mortality rate. Prevention and active treatment of organ failure can improve the outcome of patients with SAP.

关键词：急性胰腺炎器官功能衰竭危险因素治疗临床研究

Genetic Algorithms Applied to Multi-Class Clustering for Gene Ex-pression Data

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Genomics, Proteomics & Bioinformatics 2003年第4期1卷 279-287页

作者： HaiyanPan junzhu DanfuHan InstituteofBioinformatics ZhejiangUniversityHangzhou310029China DepartmentofMathematics ZhejiangUniversityHangzhou310027China

A hybrid GA (genetic algorithm)-based clustering (HGACLUS) schema, combining merits of the Simulated Annealing, was described for finding an optimal or near-optimal set of medoids. This schema maximized the clustering success by achieving internal cluster cohesion and external cluster isolation. The performance of HGACLUS and other methods was compared by using simulated data and open microarray gene-expression datasets. HGACLUS was generally found to be more accurate and robust than other methods discussed in this paper by the exact validation strategy and the explicit cluster number.

关键词： genetic algorithms gene expression clustering medoid

Engineering Multipedal DNA Walker on Paper for Sensitive Electrochemical Detection of Plant MicroRNA

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Chinese Journal of Chemistry 2022年第23期40卷 2808-2814页

作者： Xiaojuan Liu junzhu Xiang Hao Cheng Yuying Wang Feng Li College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdaoShandong 266109China College of Plant Health&Medicine Qingdao Agricultural UniversityQingdaoShandong 266109China

MicroRNAs (miRNAs) are vital regulators in both plants and animals. Therefore, it is highly desirable to develop portable and user-friendly biosensors for convenient and sensitive detection of miRNAs. Herein, a novel paper-based electrochemical biosensor was intelligently engineered for the detection of plant miRNA based on a smart DNA walking machine and λ-exonuclease (λ-Exo)-assisted target recycling amplification. Using TaMIR5086 as a target plant miRNA, the presence of TaMIR5086 could initiate the target recycling process and activate the DNA walker to move along the track on the paper. Then, numerous electroactive molecules-labeled single-strand DNA (ssDNA) would be released and adsorbed onto the surface of screen-printed electrode, generating a remarkably increased electrochemical signal. Benefitting from the dual amplification, the developed biosensor exhibits excellent analytical performance toward TaMIR5086 with a detection limit down to 0.37 pmol/L. Furthermore, the paper-based biosensor could be applied for the analysis of target miRNA in complex biological samples, which found great potential in the fields of miRNA analysis and plant biology research.

关键词： RNA recognition Electrochemistry Sensor DNA walker on paper Target recycling amplification

Chemico-biological conversion of carbon dioxide

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Journal of Energy Chemistry 2024年第2期89卷 371-387,I0009页

作者： Liangwei Hu junzhu Yang Qi Xia Jin Zhang Hongxin Zhao Yuan Lu Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou 310018ZhejiangChina Key Laboratory of Industrial Biocatalysis Ministry of EducationTsinghua UniversityBeijing 100084China Department of Chemical Engineering Tsinghua UniversityBeijing 100084China College of Biological and Chemical Engineering Jiaxing UniversityJiaxing 314000ZhejiangChina

The unabated carbon dioxide(CO_(2))emission into the atmosphere has exacerbated global climate change,resulting in extreme weather events,biodiversity loss,and an intensified greenhouse *** address these challenges and work toward carbon(C)neutrality and reduced CO_(2)emissions,the capture and utilization of CO_(2)have become imperative in both scientific research and *** cutting-edge approach to achieving efficient catalytic performance involves integrating green bioconversion and chemical *** innovative strategy offers several advantages,including environmental friendliness,high efficiency,and *** study provides a comprehensive review of existing technical routes for carbon sequestration(CS)and introduces two novel CS pathways:the electrochemicalbiological hybrid and artificial photosynthesis *** also thoroughly examines the synthesis of valuable Cnproducts from the two CS systems employing different catalysts and *** both systems heavily rely on electron transfer,direct and mediated electron transfer has been discussed and summarized in ***,this study explores the conditions suitable for different catalysts and assesses the strengths and weaknesses of *** also explored the biocompatibility of the electrode materials and developed novel *** materials were specifically engineered to combine with enzymes or microbial cells to solve the biocompatibility problem,while improving the electron transfer efficiency of ***,this review summarizes the relevant systems developed in recent years for manufacturing different products,along with their respective production efficiencies,providing a solid database for development in this *** novel chemical-biological combination proposed herein holds great promise for the future conversion of CO_(2)into advanced organic ***,it offers exciting prospects for utilizing CO_(2)in synthesizing

关键词： Carbon dioxide Bioelectric synthesis Artificial photosynthesis Synthetic product

Influence of Al_(2)O_(3)/SiO_(2) Ratio on the Structure and Properties of Na^(+)/K^(+)Ion Exchange Na_(2)O-MgO-Al_(2)O_(3)-SiO_(2) Glasses

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Journal of Wuhan University of Technology(Materials Science) 2024年第3期39卷 606-612页

作者：吴建磊 CHEN junzhu TIAN Xiaokun LI Jiahao GAO Wenkai YUE Yunlong 康俊峰 School of Materials Science and Engineering University of JinanJinan 250022China CDGM GLASS Co.LTD Chengda 610199China

In this work,the structure,viscosity and ion-exchange process of Na_(2)O-MgO-Al_(2)O_(3)-SiO_(2) glasses with different Al_(2)O_(3)/SiO_(2) molar ratios were *** results showed that,with increasing Al_(2)O_(3)/SiO_(2) ratio,the simple structural units Q_(1) and Q_(2) transformed into highly aggregated structural units Q_(3) and Q_(4),indicating the increase of polymerization degree of glass ***,the coefficient of thermal expansion decreased from 9.23×10^(-6)℃^(-1) to 8.88×10^(-6)℃^(-1).The characteristic temperatures such as melting,forming,softening and glass transition temperatures increased with the increase of Al_(2)O_(3)/SiO_(2) ratio,while the glasses working temperature range became *** increasing Al_(2)O_(3)/SiO_(2) ratio and prolonging ion-exchange time enhanced the surface compressive stress(CS)and depth of stress layer(DOL).However,the increase of ion exchange temperature increased the DOL and decreased the CS affected by stress *** was a good linear relationship between stress relaxation and surface compressive *** strengthening significantly improved the hardness of glasses,which reached the maximum value of(622.1±10)MPa for sample with Al_(2)O_(3)/SiO_(2) ratio of 0.27 after heat treated at 410℃for 2 h.

关键词： network structure viscosity ion exchange aluminosilicate glass

A Shifting Strategy for Electric Commercial Vehicles Considering Mass and Gradient Estimation

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Computer Modeling in Engineering & Sciences 2023年第10期137卷 489-508页

作者： Weiguang Zheng junzhu Zhang Shanchao Wang Gaoshan Feng Xiaohong Xu Qiuxiang Ma School of Mechanical and Electrical Engineering Guilin University of Electronic TechnologyGuilin541004China Commercial Vehicle Technology Center Dongfeng Liuzhou Automobile Co.Ltd.Liuzhou545005China School of Mechanical and Automotive Engineering Guangxi University of Science and TechnologyLiuzhou545005China

The extended Kalman filter (EKF) algorithm and acceleration sensor measurements were used to identify vehiclemass and road gradient in the work. Four different states of fixed mass, variable mass, fixed slope and variableslope were set to simulate real-time working conditions, respectively. A comprehensive electric commercial vehicleshifting strategy was formulated according to the identification results. The co-simulation results showed that,compared with the recursive least square (RLS) algorithm, the proposed algorithm could identify the real-timevehicle mass and road gradient quickly and accurately. The comprehensive shifting strategy formulated had thefollowing advantages, e.g., avoiding frequent shifting of vehicles up the hill, making full use ofmotor braking downthe hill, and improving the overall performance of vehicles.

关键词： EKF algorithm electric commercial vehicle vehicle mass road gradient comprehensive shifting strategy