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Primary pulmonary lymphoepithelioma-like carcinoma combined with situs inversus totalis

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Chinese Medical Journal 2019年第2期132卷 223-226页

作者： Tao Zhg Zhi-Hong Chen Shuo Tang xian-jun yang Shun Fang Huo-Jin Deng Department of Respiratory Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China Department of Respiratory Zhongshan HospitalShanghai Institute of Respiratory DiseaseFudan UniversityShanghai 200032China Department of Orthopaedics The Eighth Affiliated HospitalSun Yat-sen UniversityShenzhenGuangdong 517000China Department of Pathology Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong 510280China Department of Respiratory Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong 510280China

To the Editor:According to World Health Organization (WHO) histologic classification of lung tumors, primary pulmonary lymphoepithelioma-like carcinoma (LELC), belonging to the other and unclassified carcinoma, is con... 详细信息

关键词： World Health histologic primary pulmonary

Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon(Columba livia)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 511-523页

作者： Ming-xia CHEN xiang-guang LI jun-xian yang Chun-qi GAO Bin WANG Xiu-qi WANG Hui-chao YAN College of Animal Science South China Agricultural University/Key Laboratory of Chicken GeneticsBreeding and ReproductionMinistry of Agriculture/Guangdong Provincial Key Laboratory of Agro-Animal Genomics/South China Collaborative Innovation Center for Poultry Disease Control and Product Safety Guangdong Fengli Agricultural Comprehensive Development Co. Ltd.

The objective of this study was to investigate the relationship between gene expression of nutrient（amino acid, peptide, sodium and proton） transporters in the small intestine and embryonic growth in domestic pigeons（Columba livia）. One hundred and twenty-five fertilized eggs were randomly assigned into five groups and were incubated under optimal conditions（temperature of 38.1 °C and relative humidity of 55%）. Twenty embryos/birds from each group were sacrificed by cervical dislocation on embryonic day（E） 9, 11, 13, 15 and day of hatch（DOH）. The eggs, embryos（without yolk sac）, and organs（head, brain, heart, liver, lungs, kidney, gizzard, small intestine, legs, and thorax） were dissected, cleaned, and weighed. Small intestine samples were collected for RNA isolation. The m RNA abundance of intestinal nutrient transporters was evaluated by real-time reverse transcription-polymerase chain reaction（RT-PCR）. We classified these ten organs into four types according to the changes in relative weight during embryonic development. In addition, the gene expression of nutrient transporters was differentially regulated by embryonic day. The m RNA abundances of b^0,＋AT, EAAT3, y^＋LAT2, Pep T1, LAT4, NHE2, and NHE3 increased linearly with age, whereas m RNA abundances of CAT1, CAT2, LAT1, EAAT2, SNAT1, and SNAT2 were increased to higher levels on E9 or E11 and then decreased to lower levels until DOH. The results of correlation analysis showed that the gene expressions of b^0,＋AT, EAAT3, Pep T1, LAT4, NHE2, NHE3, and y^＋LAT2 had positive correlations with body weight（0.71〈correlation coefficient（CC）〈0.82, P〈0.0001）, while CAT1, CAT2, EAAT2, SNAT1, and SNAT2 had negative correlations with body weight（-0.86〈CC〈-0.64, P〈0.0001）. The gene expressions of b^0,＋AT, EAAT3, LAT4, Pep T1, NHE2, NHE3, and y^＋LAT2 showed positive correlations with intestinal weight（0.80〈CC〈0.91, P〈0.0001）, while CAT1, CAT2, and EAAT2 showed negative correlations with intestinal weight（-0.84〈CC〈-0.67, P〈0.0

关键词： Embryonic growth Nutrient transporters Gene expression Small intestine Pigeons

Effects of promoters, the Woodchuck hepatitis post-transcriptional regulatory element, and weakened selection markers on transgene expression in transfected Chinese hamster ovary cells

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Effects of promoters, the Woodchuck hepatitis post-transcrip...

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Tian-Yun Wang Wen Wang Si-Jia Chen Qin Li Fang Wang Li Wang xian-jun yang Department of Biochemistry and Molecular Biology Xinxiang Medical University International Joint Research Laboratory for Recombiant Pharmaceutical Protein Expression System of Henan Xinxiang Medical University College of Pharmacy Xinxiang Medical University

Chinese hamster ovary(CHO) cells are the host of choice for the production of recombinant proteins in mammalian expression ***,we investigated the effects of promoters,the Woodchuck hepatitis posttranscriptional regulatory element(WPRE),and selection markers on transgene expression in CHO *** cytomegalovirus major(CMV) and Simian virus 40(SV40) promoters,were combined with the G418 weakened resistance gene and WPRE cisacting element to generate the expression *** results showed that the G418 weakened resistance gene increased transgene expression driven by the CMV and SV40 ***,WPRE combined with the G418 resistance gene obviously upregulated gene expression in CHO ***,WPRE showed no enhancing effects when the G418 weakened resistance gene was used,suggesting that the effects of WPRE were selection marker *** addition,WPRE combined with the G418 resistance gene showed higher translational efficiency and transient transgene expression compared with the control ***,analysis of cetuximab expression also showed that the CMV promoter combined with WPRE expression produced higher protein levels than the CMV promoter *** conclusion,the CMV promoter combined with the WPRE had positive effects on the regulation of CHO expression and could be used for recombinant protein production.

关键词： Chinese hamster ovary WPRE Promoter Weakened Resistance gene Transgene

来源： cnki会议评论

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全反式维甲酸对K562细胞的诱导分化作用

中国当代儿科杂志 2003年第1期5卷 8-11页

作者：袁晓军廖清奎罗春华赵玉红李丰益周密 xian-jun yang 华西医科大学附属第二医院儿科成都四川610041 Department of Pediatrics Second Affiliated Hospital West China University of Medical Sciences Chengdu 610041 China

目的全反式维甲酸(ATRA)对肿瘤细胞具有广泛的抑制增殖、促进分化作用。本研究通过体外培养检测ATRA对K562细胞是否具有诱导分化作用。方法采用联苯胺染色、瑞氏染色、非特异性酯酶染色、硝基四唑氮蓝还原试验4种不同的染色方法及流... 详细信息

目的全反式维甲酸(ATRA)对肿瘤细胞具有广泛的抑制增殖、促进分化作用。本研究通过体外培养检测ATRA对K562细胞是否具有诱导分化作用。方法采用联苯胺染色、瑞氏染色、非特异性酯酶染色、硝基四唑氮蓝还原试验4种不同的染色方法及流式细胞术,观察经1μmol/L及2.5μmol/L ATRA诱导1d,4d,5d后,K562细胞出现诱导分化特征。结果 1μmol/L及2.5μmol/L ATRA均可诱导K562细胞向粒系分化。培养4d,1μmol/L ATRA组61.5％的K562细胞、2.5μmol/L组39％的K562细胞显示出向粒系成熟方向分化;培养5d,处理组的分化细胞数仍明显高于对照组(P0.05)。且均未出现向红系或单核细胞方向分化的特征。流式细胞仪检测ATRA诱导前后CD13及CD71的表达,1μmol/L ATRA诱导K562细胞1d,CD13抗原表达阳性率为8.0％,2.5μmol/L组则为6.7％,均高于对照组(2.1％)。培养5d,1μmol/L和2.5μmol/L AFRA组的CD13分别升高到28.1％,37.8％,而CD71则分别下降至1.2％和0.9％。与对照组比差异均具有显著性(P<0.05)。结论 ATRA可诱导K562细胞向粒系成熟方向分化。

关键词：全反式维甲酸白血病细胞 K562 诱导分化

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献县家蝇对常用杀虫剂抗药性调查及防制对策

医学动物防制 2016年第10期32卷 1166-1167页

作者：李俊杰赵俊先杨刚献县疾病预防控制中心河北062250

目的了解献县家蝇对溴氰菊酯等常用杀虫剂的抗性现状,为合理选用化学杀虫剂、指导家蝇防制提供科学依据。方法采用点滴法,测定家蝇半数致死量(LD50)。结果献县家蝇对4种常用杀虫剂的LD50依次为溴氰菊酯0.49 590μg/只、高效氯氰菊酯0.01... 详细信息

目的了解献县家蝇对溴氰菊酯等常用杀虫剂的抗性现状,为合理选用化学杀虫剂、指导家蝇防制提供科学依据。方法采用点滴法,测定家蝇半数致死量(LD50)。结果献县家蝇对4种常用杀虫剂的LD50依次为溴氰菊酯0.49 590μg/只、高效氯氰菊酯0.01 990μg/只,敌敌畏0.05 376μg/只,残杀威0.03 393μg/只,与敏感试虫相比,抗性系数(R/S值)溴氰菊酯为43.50倍、高效氯氰菊酯为33.17倍、敌敌畏为6.48倍、残杀威为54.73倍。结论献县家蝇对4种杀虫剂均产生不同程度的抗药性,与卫生城市创建等工作中杀虫剂的不合理使用有关;建议加强抗性监测,合理选择用药,采取综合防治措施,以缓解家蝇抗药性的发展。

关键词：家蝇抗药性杀虫剂防制对策

双交联网络有机硅弹性体的制备及其自修复性能研究

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高分子学报 2020年第6期51卷 656-669,I0006页

作者：刘珠洪鹏向洪平黄梓英罗青宏杨先君刘晓暄广东省功能软凝聚态物质重点实验室广东工业大学材料与能源学院广州510006

首先通过水解缩合法制备出了不同巯基含量的无色透明巯基硅油(PDMS-SH),然后通过PDMS-SH与端乙烯基硅油的光诱导点击反应和羧基硅油与氨基硅油的热可逆动态离子交联构建出可逆/不可逆杂化双交联网络,制备出一种可快速UV固化及优异自修... 详细信息

首先通过水解缩合法制备出了不同巯基含量的无色透明巯基硅油(PDMS-SH),然后通过PDMS-SH与端乙烯基硅油的光诱导点击反应和羧基硅油与氨基硅油的热可逆动态离子交联构建出可逆/不可逆杂化双交联网络,制备出一种可快速UV固化及优异自修复性的有机硅透明弹性体.辐照强度为70 mW/cm^2,Darocur1173加入量为1.0 wt%,―SH/―Vi摩尔比为1.5/1时,巯基-烯点击聚合具有较高的凝胶率、转化率及聚合速率,且固化速率不受离子交联网络的影响.巯基-烯初网络形成后,进一步加热可促进离子网络的形成,提高弹性体力学性能.此外,动态离子交联网络在弹性体中均匀分布.增加离子交联网络可有效降低松弛活化能,有利于应力松弛及动态可逆性,同时热处理工艺有利于动态离子网络的移动及解离-重组过程,从而有利于双网络结构的形成及修复效率的提升.更重要的是,选用巯基硅油DE/15及离子交联网络含量仅15 wt%时,弹性体具有较优力学性能及自修复效率,多次修复后的修复效率仍可高达90%以上,同时快速固化的弹性体具有高达90%以上的可见光透光率.为基于可逆动态离子缔合诱导的快速固化自修复透明有机硅材料提供一种新型可行的制备方法.

关键词：巯基-烯点击光聚合动态离子交联杂化双网络自修复有机硅弹性体巯基硅油

时频交叠LFMCW信号的PAHT检测与频移分离

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科学技术与工程 2016年第36期16卷 184-189页

作者：熊智威高宪军杨承志吴宏超空军航空大学长春130022

为有效解决时频交叠周期LFMCW信号分离的难题,首先提出一种基于周期AHT(PAHT)的检测算法,通过对匹配函数进行改进,有效积累截获到的交叠多周期能量实现高概率检测,然后将PAHT积累得到的强信号解线调,根据此时的频率分布特性近似量化频率... 详细信息

为有效解决时频交叠周期LFMCW信号分离的难题,首先提出一种基于周期AHT(PAHT)的检测算法,通过对匹配函数进行改进,有效积累截获到的交叠多周期能量实现高概率检测,然后将PAHT积累得到的强信号解线调,根据此时的频率分布特性近似量化频率,估计频率量化点与信号频率的偏离差值,再通过频移改善窄带频域滤波分离信号的效果;最后利用IFFT恢复信号,依次循环完成交叠强弱信号的分离。仿真结果对算法的检测效果和复杂度进行了分析,并在验证分离效果的同时采取了两种方法对信号的滤波质量进行了评价,验证了其在低信噪比下的可行性。

关键词：信号分离信号检测周期模糊函数霍夫变换频率偏移时频交叠

二氧化碳诱蚊灯法和栖息蚊虫捕捉法成蚊监测效果比较

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中国国境卫生检疫杂志 2016年第5期39卷 333-335页

作者：赵锋陈琳王俊贤杨雨樊学军四川出入境检验检疫局四川成都610041

目的比较二氧化碳诱蚊灯法和栖息蚊虫捕捉法捕获成蚊的差异,探索两种方法在成蚊监测中的作用。方法 2014年5-10月在四川双流国际机场范围内4种生境,采用二氧化碳诱蚊灯法和栖息蚊虫捕捉法进行成蚊监测调查。结果二氧化碳诱蚊灯法捕获成... 详细信息

目的比较二氧化碳诱蚊灯法和栖息蚊虫捕捉法捕获成蚊的差异,探索两种方法在成蚊监测中的作用。方法 2014年5-10月在四川双流国际机场范围内4种生境,采用二氧化碳诱蚊灯法和栖息蚊虫捕捉法进行成蚊监测调查。结果二氧化碳诱蚊灯法捕获成蚊225只,其中雌蚊214只,占95.11%;栖息蚊虫捕捉法捕获成蚊322只,其中雌蚊282只,占87.58%。统计分析显示,两种方法捕获的致倦库蚊雌蚊、三带喙库蚊雌蚊、白纹伊蚊雌蚊数量差异没有统计学意义。两种方法在机场住宿区、候机楼入境关口、国际货站货运区捕获蚊类数量差异没有统计学意义,而在污水处理厂办公区捕获蚊类数量差异有统计学意义。从捕获成蚊种类来看,二氧化碳诱蚊灯法没有捕获骚扰阿蚊、中华按蚊等当地稀少种类。两种监测方法监测的蚊虫季节消长趋势基本一致。结论二氧化碳诱蚊灯法比栖息蚊虫捕捉法更稳定,更适用于监测;在本底调查时,应采用多种方法,互相配合。

关键词：二氧化碳诱蚊灯法栖息蚊虫捕捉法成蚊

Detection and evaluation of the mutations of embB gene in etham butol-susceptible and resistant Mycobacterium tuberculos isisolates from China

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中华医学杂志(英文版) 2005年第20期000卷 69-71页

作者： WU Xue-qiong, LIANG Jian-qin, ZHANG jun-xian, LU yang, LI Hong-min, ZHANG G uang-yu, YAN Guo-rui and DING Bei-chuan Tuberculosis Research Laboratory, Tuberculosis Centre, 309th Hospital of PLA , Beijing 100091, China Clinical Laboratory, Thorax Disease Hospital of Hebei Province, Shijiazhuang 050 041, China Clinical Laboratory, Thorax Disease Hospital of Henan Province, Zhengzhou 450003 , China Clinical Laboratory, Research Institute of TB Control and Prevention of Beijing, Beijing 100035, China Tuberculosis Research Laboratory Tuberculosis Centre 309th Hospital of PLA Beijing 100091 China Clinical Laboratory Thorax Disease Hospital of Hebei Province Shijiazhuang 050041 China Clinical Laboratory Thorax Disease Hospital of Henan Province Zhengzhou 450003 China Clinical Laboratory Research Institute of TB Control and Prevention of Beijing Beijing 100035 China