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MiR-106b-5p Inhibits Tumor Necrosis Factor-α-induced Apoptosis by Targeting Phosphatase and Tensin Homolog Deleted on Chromosome 10 in Vascular Endothelial Cells

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Chinese Medical Journal 2016年第12期129卷 1406-1412页

作者： Jing Zhang Su-Fang Li Hong Chen jun-xian song Department of Cardiology Peking University People's Hospital Beijing 100044 China Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction Peking University People's Hospital Beijing 100044 China Center for Cardiovascular Translational Research Peking University People's Hospital Beijing 100044 China

Background： Apoptosis of endothelial cells （ECs） plays a key role in the development of atherosclerosis and there are also evidence indicated that phosphatase and tensin homolog deleted on chromosome 10 （PTEN） is a viable target in therapeutic approaches to prevent vascular ECs apoptosis. Aberrant miR-106b-5p expression has been reported in the plasma of patients with unstable atherosclerotic plaques. However, the role and underlying mechanism of miR-106-5p in the genesis of atherosclerosis have not been addressed. In this study, we explored the anti-apoptotic role of miR-106-5p by regulating PTEN expression in vascular ECs. Methods： Real-time reverse transcription polymerase chain reaction （RT-PCR） was performed to detect the expression levels of miR-106b-5p in human atherosclerotic plaques and normal vascular tissues. Human umbilical vein endothelial cells （HUVEC） were transfected with miR-106b-5p mimic or negative control mimic, and apoptosis was induced by serum starvation and tumor necrosis factor-α （TN F-α） treat. Western blotting and real-time RT-PCR experiments were used to detect PTEN expression levels and TN F-α-induced apoptosis was evaluated by the activation of caspase-3 and cell DNA fragmentation levels in HUVEC. Results： The expression ofmiR-106b-5p was significantly downregulated in plaques than in normal vascular tissues. TNF-α significantly downregulated miR-106b-5p expression levels and upregulated activation of caspase-3 and cell DNA fragmentation levels in HUVEC. Overexpression ofmiR-106b-5p with miR-106b-5p mimic inhibited PTEN expression and TNF-α-induced apoptosis in HUVEC. Luciferase reporter assays confirmed that miR-106b-5p binds to PTEN mRNA 3＇ untranslated region site, Conclusion： MiR-106b-5p could inhibit the expression of PTEN in vascular ECs, which could block TNF-α-induced activation of caspase-3, thus prevent ECs apoptosis in atherosclerosis diseases.

关键词： Apoptosis Atherosclerosis MicroRNAs Phosphatase and Tensin Homolog Deleted on Chromosome 10

Angiotensin-（1-7）： new perspectives in atherosclerosis treatment

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Journal of Geriatric Cardiology 2015年第6期12卷 676-682页

作者： Feng ZHANG jun LIU Su-Fang LI jun-xian song Jing-Yi REN Hong CHEN Department of Cardiology Peking University People's Hospital Beijing China William Harvey Research Institute Barts and the London School of Medicine and Dentistry Queen Mary University of London London United Kingdom

血管收缩素(Ang )-(1-7) 在高血压蛋白原酶血管收缩素系统(地岬) 作为新 bioactive 肽被认出。Ang-(1-7 ) 是看起来对心血管的疾病保护的 Ang-II 的一个柜台规章的调停人。最近的研究发现了 Ang-(1-7 ) 在减少光滑的肌肉房间增长和移植... 详细信息

血管收缩素(Ang )-(1-7) 在高血压蛋白原酶血管收缩素系统(地岬) 作为新 bioactive 肽被认出。Ang-(1-7 ) 是看起来对心血管的疾病保护的 Ang-II 的一个柜台规章的调停人。最近的研究发现了 Ang-(1-7 ) 在减少光滑的肌肉房间增长和移植起了一个重要作用，改进 endothelial 功能并且调整类脂化合物新陈代谢，导致动脉粥样硬化患者损害的抑制和匾稳定性的增加。尽管 Ang-(1-7 ) 的临床的申请由于它的 pharmacokinetic 性质被限制，包括更稳定的类似物和特定的交货混合物，稳定的混合物的鉴定启用了 Ang-(1-7 ) 的临床的申请。在这评论，我们在动脉粥样硬化开发期间有关 Ang-(1-7 ) 和相关机制的生物角色讨论了最近的调查结果。另外，我们加亮观点用 Ang-(1-7 ) 开发治疗学的策略对待动脉粥样硬化。

关键词：动脉粥样硬化肾素-血管紧张素系统治疗临床应用生物活性肽心血管疾病动力学特性稳定性

Predictors and in-hospital prognosis of recurrent acute myocardial infarction

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Journal of Geriatric Cardiology 2016年第10期13卷 836-839页

作者： Cheng-Fu CAO Su-Fang LI Hong CHEN jun-xian song Department of Cardiology Peking University People's Hospital Beijing China Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction Peking University People's Hospital Beijing China Center for Cardiovascular Translational Research Peking University People's Hospital Beijing China

为了调查作出贡献的因素和病人的在里面医院预后与或没有周期性的尖锐心肌的梗塞(AMI ) ， 1686 个连续 AMI 病人全部的 .MethodsA 承认到 2015 年 12 月的从 2010 年 1 月的医院被招募到北京大学民族。他们的临床的特征回顾地与或没有... 详细信息

为了调查作出贡献的因素和病人的在里面医院预后与或没有周期性的尖锐心肌的梗塞(AMI ) ， 1686 个连续 AMI 病人全部的 .MethodsA 承认到 2015 年 12 月的从 2010 年 1 月的医院被招募到北京大学民族。他们的临床的特征回顾地与或没有周期性的 AMI 在病人之间被作比较。然后 multivariable 逻辑回归被用来估计周期性的心肌的 infarction.ResultsRecurrent AMI 病人的预言者更旧(69.3 #

关键词：急性心肌梗死临床特点复发 logistic回归分析预测预后冠状动脉疾病危险因素

Total ischemic time and outcomes for patients with ST-elevation myocardial infarction： does time of admission make a difference？

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Journal of Geriatric Cardiology 2016年第8期13卷 658-664页

作者： jun-xian song Li ZHU Chong-You LEE Hui REN Cheng-Fu CAO Hong CHEN Department of Cardiology Peking University People's Hospital Beijing China Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction Peking University People's Hospital Beijing China Center for Cardiovascular Translational Research Peking University People's Hospital Beijing China Department of Nephrology Peking University People's Hospital Beijing China

ObjectiveTo 调查录取时间是否与圣举起心肌的梗塞(STEMI ) 在病人与灌注治疗和在里面医院死亡的延期被联系进入紧急情况部门并且在在 2012 年 4 月和 2015 年 3 月之间的北京大学民族医院经历了主要经皮的冠的干预的有 STEMI 的 .Metho... 详细信息

ObjectiveTo 调查录取时间是否与圣举起心肌的梗塞(STEMI ) 在病人与灌注治疗和在里面医院死亡的延期被联系进入紧急情况部门并且在在 2012 年 4 月和 2015 年 3 月之间的北京大学民族医院经历了主要经皮的冠的干预的有 STEMI 的 .MethodsAll 病人被包括。我们在在病人之间的死亡在在常规小时期间承认的不值勤的时间或时期和那些期间承认了的临床的特征，全部的 ischemic 时间，和在里面医院检验了差别。Multivariate 逻辑回归被用来估计在不值勤的时间或时期录取和临床的 outcome.ResultsThe 样品之间的关系包括了 184 和 105 个病人， STEMI 分别地在不值勤的时间或时期和常规小时期间承认了到医院。全部的 ischemic 和 onset-to-door 时间在比在在常规小时期间承认的那些之中在不值勤的时间或时期期间招收的病人是显著地更短的(所有 P <；0.05 ) 。Door-to-balloon (DTB ) 时间， DTB 时间 ≤ 的率；90 min，和在里面医院死亡在组之间是可比较的。Multivariate 逻辑回归显示出那年龄和 creatinine 水平，然而并非不值勤的时间或时期承认，与增加的在里面医院 death.ConclusionsOff 小时承认独立地被联系没导致推迟的灌注在有 STEMI 的病人的治疗或增加的在里面医院死亡。进一步的努力应该集中于识别与医院前和灌注治疗的在里面医院延期联系的枢轴的因素，并且为灌注节目实现优秀改进行动。

关键词：在里面医院死亡心肌的梗塞不值勤的时间或时期承认经皮的冠的干预总计 ischemic 时间

Characteristics of elevated cardiac troponin I in patients with acute ischemic stroke

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Journal of Geriatric Cardiology 2017年第6期14卷 401-406页

作者： Yu-Xia CUI Hui REN Chong-You LEE Su-Fang LI jun-xian song Xu-Guang GAO Hong CHEN Department of Cardiology Peking University People's Hospital Beijing China Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction Peking University People's Hospital Beijing China Center for Cardiovascular Translational Research Peking University People's Hospital Beijing China Department of Neurology Peking University People's Hospital Beijing China

ObjectiveTo 学习心脏的 troponin 的预示的特征我(cTnI ) 在尖锐 ischemic stroke.MethodsWe 的举起回顾地学习了病人(n = 248 ) 与尖锐 ischemic 击，尖锐圣片断举起心肌的梗塞，和尖锐 non-ST-elevation 心肌的梗塞在 2013 年 1 月和... 详细信息

ObjectiveTo 学习心脏的 troponin 的预示的特征我(cTnI ) 在尖锐 ischemic stroke.MethodsWe 的举起回顾地学习了病人(n = 248 ) 与尖锐 ischemic 击，尖锐圣片断举起心肌的梗塞，和尖锐 non-ST-elevation 心肌的梗塞在 2013 年 1 月和 2015 年 10 月之间被对待。在在这三个组之中的 cTnI 层次的基线人口统计的数据和变化被比较。有尖锐 ischemic 击的病人被分到 cTnI 举起组也(cTnI > 0.034 ng/mL ) 或没有 cTnI 举起组(cTnI 0.034 ng/mL ) 。逻辑回归分析被用来识别与尖锐 ischemic 击在病人与提高的浆液 cTnI 联系的风险因素。而且，医院停留和主要心血管的结果的发生的持续时间与尖锐 ischemic 击在病人被作比较，与尖锐 ischemic 击与这或没有提高的 cTnI.ResultsIn 学习病人的人口( n = 178 )，尖锐圣片断举起心肌的梗塞( n = 35 )，并且尖锐 non-ST-elevation 心肌的梗塞( n = 35 )，有有提高的 cTnI 的尖锐 ischemic 击的病人包括了18.5%题目。有提高的 cTnI 的病人更老、更可能有高血压的历史。另外，这些病人有煽动性的标记，减少的肾的功能，增加的 D 暗淡层次，更高的 NIH 击分数，和更低的左室的喷射部分的高水平。逻辑回归分析两个都显示出那百分比嗜中性并且 NIH 击分数被提高；估计的 glomerular 过滤率和左室的喷射部分与尖锐 ischemic 击在病人被减少提高了 cTnI，并且他们在与尖锐 ischemic 击在病人检测的医院 stay.ConclusionElevated cTnI 期间有更经常的主要心血管的事件，在医院停留期间显示了差的短期的预后的更大的可能性的。

关键词：心肌肌钙蛋白i 脑卒中 logistic回归分析急性患者缺血 Logistic回归分析心肌肌钙蛋白I

Effect of Endothelial Microparticles Induced by Hypoxia on Migration and Angiogenesis of Human Umbilical Vein Endothelial Cells by Delivering MicroRNA-19b

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Chinese Medical Journal 2018年第22期131卷 2726-2733页

作者： Hui-Zhu Liang Su-Fang Li Feng Zhang Man-Yan Wu Chang-Long Li jun-xian song Chongyou Lee Hong Chen Department of Cardiology Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial InfarctionCenter for Cardiovascular Translational ResearchPeking University People's HospitalBeijing 100044China

Background: Microparticles (MPs) are small extracellular plasma membrane particles shed by activated and apoptotic cells, which are involved in the development of atherosclerosis. Our previous study found that microRNA (miR)-19b encapsulated within endothelial MPs (EMPs) may contribute to the upregulation of circulating miR-19b in unstable angina patients. Hypoxia is involved in atherosclerosis as a critical pathological stimulus. However, it still remains unclear whether the increase of miR-19b levels in EMPs is related to hypoxia and if the effect of miR-19b - wrapped within EMPs - stimulates hypoxia on vascular endothelial cells. This study aimed to explore the changes of miR-19b in EMPs induced by hypoxia as well as their effects on endothelial cells.Methods: Human umbilical vein endothelial cells (HUVECs) were culturedin vitro and arranged to harvest EMPs in two parts: the first part consisted of EMPcontrol and EMPhypoxia and the second part included EMPvehicle, EMPNC mimic, and EMPmiR-19b mimic. Cell migration was detected by scratch migration and transwell chamber migration. Angiogenesis was assessed by tube formation assays. Furthermore, we predicted the target gene of miR-19b by bioinformatics analysis, and luciferase assay was used to verify the targeted gene of miR-19b. Data were analyzed by one-way analysis of variance. Student’st-test was used when two groups were compared.Results: Compared with EMPcontrol- and EMPhypoxia-inhibited migration of cells by scratch migration assay (80.77 ± 1.10 vs. 28.37 ± 1.40,P < 0. 001) and transwell chamber migration assay (83.00 ± 3.46 vs. 235.00 ± 16.52,P < 0.01), the number of tube formations was markedly reduced by 70% in the EMPhypoxia group (P < 0.001)in vitro analysis of HUVECs. Meanwhile, a strong inhibition of migration and tube formation of HUVECs in the presence of miR-19b-enriched EMPmiR-19b mimic was observed. This effect might be due to the delivery of miR-19b in EMPs. Transforming growth factor-β2 (TGFβ2) was predicted to be one of the target genes of

关键词： Endothelial Microparticle MicroRNA-19b Hypoxia Cell Migration Angiogenesis Transforming Growth Factor-β2

Clopidogrel induced arthritis and fever

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Journal of Geriatric Cardiology 2017年第6期14卷 427-427页

作者： Cheng-Fu CAO Feng ZHANG Hong Chen jun-xian song Chuan-Fen LIU Su-Fang LI Chong-You LEE Department of Cardiology Peking University People's Hospital Beijing China Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction Peking University People's Hospital Beijing China Center for Cardiovascular Translational Research Peking University People's Hospital Beijing China

Clopidogrel induced arthritis and fever was a very scarce drug side effect. These may be the manifestation of clopidogrel hypersensitivity. There were several previously reported cases. After reviewing these reports, the most common characteristics of clopidogrel hypersensitivity include (1) fever, (2) rash, (3) pruritis and (4) acute arthritis.

关键词：氯吡格雷关节炎冠状动脉造影发热肌钙蛋白I 介入治疗糖尿病心电图

Recent advances in vacancy engineering for reliable lithiumsulfur batteries

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Rare Metals 2024年第2期43卷 455-477页

作者： Wen-Dong Liu Xiao Tang jun-An Feng Chao-Yue Zhang Hao Liu Chuan Shi Xiao-xian Zhao Jian-jun song College of Physics School of Chemistry and Chemical EngineeringQingdao UniversityQingdao266071China Faculty of Science Centre for Clean Energy TechnologySchool of Mathematical and Physical SciencesUniversity of Technology SydneySydneyNSW2007Australia Department of Chemistry College of ScienceHebei Agricultural UniversityBaoding071001China

The high-energy-density and low-cost features endow lithium-sulfur batteries with broad application prospects.However,many drawbacks,especially the detrimental shuttle effect,have hindered the further development of LSBs.In response,a lot of new structures have been applied to suppress the shuttle effect and promote the development of LSBs.Recently,vacancy engineering has gained the attention of researchers due to its unique electronic structure.This review aims to analyze the application of vacancy engineering in LSBs.Firstly,the electrochemistry of LSBs has been systematically discussed and the existing challenges as well as improvement tactics of LSBs have also been presented.Subsequently,the preparation methods and characterization technologies of various vacancies are summarized,including oxygen vacancies,sulfur vacancies,selenium vacancies,other anion vacancies,cation vacancies,etc.The latest applications of vacancy engineering in LSBs are also summarized in this review.Finally,some prospects and insights for further investigation and practical application of vacancy engineering in LSBs are put forward.

关键词： Lithium-sulfur batteries(LSBs) Defects Vacancy engineering Electronic structure Shuttle effect

Interpretation and characterization of rate of penetration intelligent prediction model

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Petroleum Science 2024年第1期21卷 582-596页

作者： Zhi-jun Pei xian-Zhi song Hai-Tao Wang Yi-Qi Shi Shou-Ceng Tian Gen-Sheng Li College of Petroleum Engineering China University of Petroleum(Beijing)Beijing102249China State Key Laboratory of Petroleum Resources and Prospecting Beijing102249China Kunlun Digital Technology Co. Ltd.Beijing102206China

Accurate prediction of the rate of penetration(ROP)is significant for drilling optimization.While the intelligent ROP prediction model based on fully connected neural networks(FNN)outperforms traditional ROP equations and machine learning algorithms,its lack of interpretability undermines its credibility.This study proposes a novel interpretation and characterization method for the FNN ROP prediction model using the Rectified Linear Unit(ReLU)activation function.By leveraging the derivative of the ReLU function,the FNN function calculation process is transformed into vector operations.The FNN model is linearly characterized through further simplification,enabling its interpretation and analysis.The proposed method is applied in ROP prediction scenarios using drilling data from three vertical wells in the Tarim Oilfield.The results demonstrate that the FNN ROP prediction model with ReLU as the activation function performs exceptionally well.The relative activation frequency curve of hidden layer neurons aids in analyzing the overfitting of the FNN ROP model and determining drilling data similarity.In the well sections with similar drilling data,averaging the weight parameters enables linear characterization of the FNN ROP prediction model,leading to the establishment of a corresponding linear representation equation.Furthermore,the quantitative analysis of each feature's influence on ROP facilitates the proposal of drilling parameter optimization schemes for the current well section.The established linear characterization equation exhibits high precision,strong stability,and adaptability through the application and validation across multiple well sections.

关键词： Fully connected neural network Explainable artificial intelligence Rate of penetration ReLU active function Deep learning Machine learning