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无限长均匀带电直圆柱面面上电场强度分布

辽宁工程技术大学学报（自然科学版） 2007年第6期26卷 959-960页

作者：牟俊侠大连交通大学数理系辽宁大连116028

分别利用电场力做功和能量的关系以及场强叠加原理等方法,给出了计算无限长均匀带电直圆柱面面上电场强度的几种方法,从而得到了无限长均匀带电直圆柱面面上的电场强度。同时明确指出了无限长均匀带电直圆柱面面上的电场强度具有确定的... 详细信息

分别利用电场力做功和能量的关系以及场强叠加原理等方法,给出了计算无限长均匀带电直圆柱面面上电场强度的几种方法,从而得到了无限长均匀带电直圆柱面面上的电场强度。同时明确指出了无限长均匀带电直圆柱面面上的电场强度具有确定的值。并且指出现在在计算无限长均匀带电直圆柱面电场强度的分布时,忽略了其面上电场强度的讨论。建议今后对其的研究应分别从其面内、面外、带电直圆柱面面上三个方面加以讨论。

关键词：无限长均匀带电直圆柱面电场强度

罗汉果甜苷与黄芪多糖联用对小鼠骨骼肌抗氧化能力的影响

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南方农业学报 2024年第7期55卷 1925-1934页

作者：卢晓凤骆隽言韦明涵农彩密党钊林李银王倩蒋钦杨黄艳娜广西大学动物科学技术学院广西南宁530004

【目的】探讨罗汉果甜苷与黄芪多糖联用对提升小鼠骨骼肌抗氧化能力的影响,并揭示其协同作用机制,为开发罗汉果甜苷和黄芪多糖作为绿色、安全的新型饲料添加剂应用于畜禽骨骼肌抗氧化能力提升及肉品质改善提供理论依据。【方法】将40只... 详细信息

【目的】探讨罗汉果甜苷与黄芪多糖联用对提升小鼠骨骼肌抗氧化能力的影响,并揭示其协同作用机制,为开发罗汉果甜苷和黄芪多糖作为绿色、安全的新型饲料添加剂应用于畜禽骨骼肌抗氧化能力提升及肉品质改善提供理论依据。【方法】将40只SPF级昆明小鼠随机分为4组,即对照组(CK)、罗汉果甜苷组(MOG)、黄芪多糖组(APS)及罗汉果甜苷+黄芪多糖联用组(MOG+APS),每组10只。采取经口灌胃方式给药,MOG组小鼠每日灌服200 mg/kg罗汉果甜苷,APS组小鼠每日灌服200 mg/kg黄芪多糖,MOG+APS组小鼠每日灌服200 mg/kg罗汉果甜苷+200 mg/kg黄芪多糖,CK组小鼠每日灌服0.1 mL生理盐水,连续灌服4周。给药结束后,采集小鼠脏器计算脏器指数,测定血清和骨骼肌中的抗氧化酶活性,并通过实时荧光定量PCR检测小鼠骨骼肌中抗氧化相关基因及Keap1/Nrf2信号通路相关基因的表达变化。【结果】与CK组小鼠相比,MOG组、APS组和MOG+APS组小鼠的平均日采食量、平均日增重及各脏器指数均无显著变化(P>0.05)。MOG组、APS组和MOG+APS组小鼠血清和腓肠肌中的丙二醛(MDA)含量较CK组小鼠显著降低(P<0.05,下同),谷胱苷肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性则显著升高;MOG+APS组小鼠血清和腓肠肌中的MDA含量较MOG组和APS组显著降低,而GSH-Px和SOD活性显著升高。与MOG组和APS组小鼠相比,MOG+APS组小鼠腓肠肌中的GSH-Px和SOD基因相对表达量显著上升,NF-E2相关因子2基因(Nrf2)相对表达量分别显著升高19.02%和20.10%,而Kelch样环氧氯丙烷相关蛋白1基因(Keap1)相对表达量分别显著下降29.92%和24.20%。【结论】罗汉果甜苷与黄芪多糖联用能激活Keap1/Nrf2信号通路,上调SOD和GSH-Px基因表达,提高抗氧化酶活性,而增强小鼠骨骼肌抗氧化能力。罗汉果甜苷和黄芪多糖可作为绿色、安全的新型饲料添加剂,用于提升畜禽骨骼肌抗氧化能力及改善畜禽肉品质。

关键词：小鼠罗汉果甜苷黄芪多糖骨骼肌抗氧化能力 Keap1/Nrf2信号通路

The Effect of Dietary Vegetable Oilseeds Supplement on Fatty Acid Profiles in Milk Fat from Lactating Dairy Cows

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Agricultural Sciences in China 2007年第8期6卷 1002-1008页

作者： LIU Shi-jun WANG Jia-qi BU Deng-pan WEI Hong-yang ZHOU Ling-yun luo Qiu-jiang The State Key Laboratory of Animal Nutrition/Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100094 P. R. China Xinjiang Agricultural University Urumchi 830052 P.R.China

To determine the effect of dietary supplementation with vegetable oilseeds on the composition of bovine milk fatty acids （FAs）, 40 Holstein dairy cows were used with a complete randomized design. At the beginning of the experiment, the cows were 150±25 day in milk （DIM）. Total duration of the experiment was six weeks. Measurements were made during the last three weeks. Cows in four treatments were fed with a basal diet （CT） or basal diet supplemented with either whole full fat soybean （WFS）, full fat expanded soybean （FPS） or whole full fat soybean with whole cottonseed and full fat expanded soybean （MIX）. The composition of the milk fat was analyzed by gas chromatography. Relative to control, the conjugated linoleic acid （CLA） concentration in milk fat from cows on FPS was significantly increased by 83.88% （P〈 0.05）. The proportions of C12：0 were decreased by 35.7, 35.51, and 38.65% in milk fat from cows on WFS, MIX, and FPS compared with cows on CT. Similar decreases in C 14：0 were 23.83, 24.85, and 31.48% in WFS, MIX, and FPS treatments, respectively. Feeding vegetable oilseeds increased the proportion of healthy FAs （mainly CLA）, whereas decreased the concentratiofi of C12：0 and C14：0. Therefore, milk and dairy products would have higher nutritive and therapeutic value.

关键词： dairy cattle vegetable oilseed conjugated linoleic acid milk fat

Salvianolic acid A attenuates ischemia reperfusion induced rat brain damage by protecting the blood brain barrier through MMP-9 inhibition and anti-inflammation

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Chinese Journal of Natural Medicines 2018年第3期16卷 184-193页

作者： ZHANG Wen SONG jun-Ke ZHANG Xue ZHOU Qi-Meng HE Guo-Rong XU xiao-Na Yan Rong ZHOU Wen-xia DU Guan-Hua Beijing Key Laboratory of Drug Target Identification and Drug Screening Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College Department of Neuroimmunopharmacology Beijing Institute of Pharmacology and Toxicology State Key Laboratory of Toxicology and Medical Countermeasures

Salvianolic acid A （SAA） is a water-soluble component from the root of Salvia Miltiorrhiza Bge, a traditional Chinese medicine, which has been used for the treatment of cerebrovascular diseases for centuries. The present study aimed to determine the brain protective effects of SAA against cerebral ischemia reperfusion injury in rats, and to figure out whether SAA could protect the blood brain barrier （BBB） through matrix metallopeptidase 9 （MMP-9） inhibition. A focal cerebral ischemia reperfusion model was induced by middle cerebral artery occlusion （MCAO） for 1.5-h followed by 24-h reperfusion. SAA was administered intravenously at doses of 5, 10, and 20 ***-l. SAA significantly reduced the infarct volumes and neurological deficit scores. Immunohistochemical analyses showed that SAA treatments could also improve the morphology of neurons in hippocampus CA1 and CA3 regions and increase the number of neurons. Western blotting analyses showed that SAA downregulated the levels of MMP-9 and upregulated the levels of tissue inhibitor of metalloproteinase 1 （TIMP-1） to attenuate BBB injury. SAA treatment significantly prevented MMP-9-induced degradation of ZO-1, claudin-5 and occludin proteins. SAA also prevented cerebral NF-κB p65 activation and reduced inflammation response. Our results suggested that SAA could be a promising agent to attenuate cerebral ischemia reperfusion injury through MMP-9 inhibition and anti-inflammation activities.

关键词： Salvianolic acid A Ischemia MCAO Blood brain barrier NF-κB MMP-9

The Impact of Chinese Economic Increase on Economy of Northeast Asia

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Chinese Business Review 2007年第1期6卷 39-44页

作者： CHEN Zhi-jun luo xiao-yan School of Economics ＆ Management Shijiazhuang Railway Institute Shijiazhuang 050043 China）

Chinese economic total quantity has leaped to the sixth m the world because ot the tast development for nearly 30 years, and become the world economics powerful nation gradually. The Chinese economic growth has influenced tremendously on the world economic development. Being Chinese close neighbor, how has the Northeast Asia be influenced？ This paper will elaborate from the following three aspects： Chinese economic increase made China become important strength to promote the process of regional cooperation; Chinese economic increase made the pattern of Northeast Asian cooperation in economy and trade changed; competition and difference that Chinese economic increase brought to Northeast Asia. The increase of Chinese economic has positive influence on the economy of Northeast Asia, and is one of the powers causing the Northeast Asian economy to grow. At the same time, this kind of influence is mutual. In particular the economic cooperation with Japan is the key to establish the integration of the Northeast Asian economy.

关键词： economy impact Northeast Asia

大麦非特异性磷脂酶C基因家族全基因组鉴定及苗期胁迫表达分析

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生物技术通报 2024年第8期40卷 74-82页

作者：崔原瑗王昭懿白双宇任毓昭豆飞飞刘彩霞刘凤楼王掌军李清峰宁夏大学农学院银川750021

【目的】非特异性磷脂酶C(non-specific phospholipase C,NPC)是磷脂酶C的一种,在植物生长发育和响应非生物胁迫等方面发挥重要作用。在大麦全基因组范围内鉴定NPC基因家族成员,分析相关基因表达特点,为大麦NPC基因功能研究及遗传改良... 详细信息

【目的】非特异性磷脂酶C(non-specific phospholipase C,NPC)是磷脂酶C的一种,在植物生长发育和响应非生物胁迫等方面发挥重要作用。在大麦全基因组范围内鉴定NPC基因家族成员,分析相关基因表达特点,为大麦NPC基因功能研究及遗传改良提供理论依据。【方法】基于EnsemblPlants数据库收录的大麦全基因组数据,利用生物信息学的方法对大麦NPC基因家族进行鉴定,并对大麦NPC基因家族的理化特性、亚细胞定位、系统进化关系、基因结构、蛋白结构域和启动子顺势元件等进行分析。利用转录组数据和实时荧光定量PCR分析HvNPC基因在不同组织以及苗期在低温、干旱、盐胁迫下的表达特征。【结果】在大麦基因组中,共鉴定了5个非特异性磷脂酶C基因家族成员,其编码蛋白与其他植物的NPC基因结构相似,均具有Phosphoesterase结构域;HvNPC蛋白序列长度为477-553 aa,等电点为5.22-8.83,分子量为53.12-61.33 kD;亚细胞定位预测HvNPC基因定位于叶绿体、液泡膜、细胞质中;HvNPC启动子区含有大量与非生物胁迫有关的顺势作用元件。实时荧光定量PCR分析证实HvNPC基因受干旱、高盐及低温胁迫诱导表达,其中HvNPC2、HvNPC3和HvNPC5受低温和干旱胁迫诱导表达;HvNPC3和HvNPC5受盐胁迫诱导表达。【结论】在大麦基因组中共鉴定5个HvNPC基因,HvNPC基因成员具有器官表达特异性,并且不同基因对非生物胁迫响应不同。

关键词：大麦非特异性磷脂酶C 全基因组鉴定非生物胁迫表达分析

Changes in oxygen saturation can not help diagnose acute mountain sickness(AMS)： ascending to Lhasa on the Qinghai-Tibet train

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中国应用生理学杂志 2016年第6期32卷 519-524页

作者： jun-bo AN Hao-ran GU Yu WU Yong-jun luo Department of Military Medical Geography College of High Altitude Military Medicine Third Military Medical University Chongqing 400038 China Battalion 5 of Cadet Brigade Third Military Medical University Chongqing 400038 China Key Laboratory of High Altitude Medicine (Ministry of Education) Third Military Medical University Chongqing 400038 China The 12th hospital of PLA Kashi 844200 China

Objective: Acute mountain sickness(AMS) is a common condition in individuals who ascend to altitudes over 2 500 m. There is no measurements that can reliably predict or diagnose this condition. We therefore determined whether pulse oximetry data are associated with the development of AMS and can help diagnose AMS. Methods: We studied 58 young male undergraduates who traveled from Chongqing(300 m) to Lhasa(3 658 m) by train. We collected data on the ascent profiles and AMS symptoms based on the Lake Louise Score(LLS). The resting arterial oxygen saturation(R-Sp O2) and pulse rate were then measured using finger pulse oximetry. Results: In Golmud(2 800 m) and Tanggula(5 200 m), R-SpO_2 was significantly lower in the AMS group than in the group without AMS(P<0.05). However, upon arrival in Lhasa(3 658 m), the R-SpO_2 was higher in the AMS group than in the non-AMS group(P<0.05). In Tanggula, the change in the SpO_2(CR-SpO_2) in the AMS group was higher than that in the non-AMS group(P<0.05). But in Lhasa, the CR-SpO_2 in the AMS group was lower than that in the non-AMS group(P<0.05). We also monitored heart rate(HR) throughout the study. In Xining(2 200 m) and Golmud, the HRs in the AMS group were higher than those in the non-AMS group. However, the HRs in the AMS group were lower than those in the non-AMS group in Tanggula and Lhasa. Conclusion: Based on the results of this study, the R-SpO_2 graph was not consistent. We can thus conclude that the utility of SpO_2 remains limited in the diagnosis of AMS. The results suggest that using pulse oximetry to diagnose AMS is not valuable in people ascending to Lhasa on the Qinghai-Tibet train.

关键词：脉搏血氧饱和度诊断系统 AMS 高原病拉萨火车西藏青海

Developmental Gerontology and Active Population Aging in China

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China CDC weekly 2023年第8期5卷 184-187页

作者： xiaoying Zheng Yanan luo Binbin Su Ping He Chao Guo Yaohua Tian Shasha Han Chen Chen Yiran Wang Jufen Liu jun Li School of Population Medicine and Public Health Chinese Academy of Medical Sciences/Peking Union Medical CollegeBeijing MunicipalityChina Department of Global Health School of Public HealthPeking UniversityBeijing MunicipalityChina China Center for Health Development Studies Peking UniversityBeijing MunicipalityChina APEC Health Science Academy Peking UniversityBeijing MunicipalityChina Ministry of Education Key Laboratory of Environment and Health and State Key Laboratory of Environmental Health(Incubating)School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Maternal and Child Health School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Institute of Reproductive and Child Health/National Health Commission Key Laboratory of Reproductive Health Peking UniversityBeijing MunicipalityChina Department of Epidemiology and Biostatistics School of Public HealthPeking UniversityBeijing MunicipalityChina Institute of Economics Chinese Academy of Social SciencesBeijing MunicipalityChina

Population aging is an irreversible process in the development of modern society,which brings challenges to comprehensive modernized social *** aging is a“dualistic”development issue that not only leads to aging of the labor force structure but also creates new demographic *** study describes the core thoughts of developmental gerontology(DG),which provides new insight into the relationship between active aging and comprehensive governance for modernized *** development of DG will provide a feasible and sustainable path to integrate and coordinate the relationship between population aging,society,and economy.

关键词： aging irreversible Active

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边缘革蜱AQPs重组蛋白的表达及抗蜱效果

中国兽医学报 2024年第7期44卷 1466-1472,1506页

作者：伍军何文文普浩金敏石文玉马爱军罗亭祥杨德鹏巴音查汗呼尔查新疆农业大学动物医学学院新疆乌鲁木齐830052 新疆农业大学兽医学博士后流动站新疆乌鲁木齐830052

对边缘革蜱(Dermacentor marginatus)水通道蛋白(aquaporin,AQP)rDmAQP1、rDmAQP2和rDmAQP3纯化后免疫家兔,采血,进行Western blot和ELISA试验,第3次免疫2周后进行抗蜱试验。利用SDS-PAGE凝胶电泳筛选出rDmAQP1、rDmAQP2和rDmAQP3的最优表达条件,经HisSep Ni-NTA 6FF纯化柱将3种重组蛋白纯化,家兔分4组,每组3只家兔,对照组1组,免疫组3组。纯化的3种重组蛋白分别单独免疫3组家兔,在0、14、21d分别进行1次免疫,每隔7d采血1次,制备多克隆抗体,Western blot检测其反应原性,用ELISA检测其抗体效价,待抗体效价升高后开展攻蜱试验。结果显示,rDmAQP1的最优表达条件是IPTG浓度为1.0mmol/L、37℃诱导8h;rDmAQP2的最优表达条件是IPTG浓度为1.0mmol/L、37℃诱导7h;rDmAQP3的最优表达条件是IPTG浓度为1.0mmol/L、37℃条件下诱导5h。Western blot结果显示rDmAQP1、rDmAQP2和rDmAQP3都具有一定的反应原性。ELISA结果显示rDmAQP1、rDmAQP2和rDmAQP3免疫家兔的抗体效价分别为:1∶409600、1∶51200和1∶409600,rDmAQP1蛋白免疫后总的抗蜱效果为79.74%,对边缘革蜱的平均饱血蜱质量、平均卵质量及平均卵孵化率的抑制率分别为9.43%、25.17%和63.81%;rDmAQP2蛋白免疫后总的抗蜱效果为78.78%,对边缘革蜱的平均饱血蜱质量、平均卵质量及平均卵孵化率的抑制率分别为8.30%、20.14%和68.26%;rDmAQP3蛋白免疫后总的抗蜱效果为87.91%,对边缘革蜱的平均饱血蜱质量、平均卵质量及平均卵孵化率的抑制率分别为3.23%、22.47%和80.50%。本研究通过血清学试验及抗蜱试验,发现rDmAQP1、rDmAQP2和rDmAQP3都具有抗蜱疫苗候选抗原潜力,其中rDmAQP3的免疫效果最佳,为rDmAQP1、rDmAQP2和rDmAQP3功能研究奠定了基础。

关键词：边缘革蜱水通道蛋白多克隆抗体的制备抗蜱效果评价