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稻曲病菌毒素的活性测定、抗体制备与细胞定位

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实验生物学报 2004年第4期37卷 310-314页

作者：陈美军胡东维徐颖浙江大学生物技术研究所杭州310029

稻曲病菌在PD 液体培养基中生长良好,并能产生对植物细胞具有高度生物抑制活性的毒素。生物学活性测定袁明,用100%的甲醇能提取稻曲病菌液体培养物中的粗毒素。粗毒素对小麦胚根胚芽的生长有强烈的抑制作用。把毒素主要成分Ustiloxin A ... 详细信息

稻曲病菌在PD 液体培养基中生长良好,并能产生对植物细胞具有高度生物抑制活性的毒素。生物学活性测定袁明,用100%的甲醇能提取稻曲病菌液体培养物中的粗毒素。粗毒素对小麦胚根胚芽的生长有强烈的抑制作用。把毒素主要成分Ustiloxin A 和BSA 偶联后,制备了抗血清,ELISA 检测表明用两种偶联剂偶联所制备的抗体效价分别为1∶20000和1∶6000。进一步的免疫胶体金标记分析表明,所制备的抗体能与茼丝中分泌的毒素特异性结合,说明所获得的抗体是特异性的。

关键词：稻曲病菌毒素活性抗体细胞定位免疫金标记

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Integration of perioperative features and intragraft TCF7L2 expression to predict lipid metabolic disorder in liver transplant recipients

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Science China Life Sciences 2025年第1期 297-299页

作者： junbin Zhou Rongli wei Guanghan Fan Zhengxing Lian xuanyu Zhang Linsong Tang Zhetuo Qi Haiyang Xie Shusen Zheng Qiang wei Xiao xu Department of Hepatobiliary and Pancreatic Surgery People's Hospital of LishuiAffiliated Lishui Hospital of Wenzhou Medical UniversityThe First Affiliated Hospital of Lishui University NHFPC Key Laboratory of Combined Multi-Organ Transplantation Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province Department of Hepatobiliary and Pancreatic SurgeryHangzhou First People's Hospital Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of Medicine Department of Urology The Second Affiliated HospitalZhejiang University School of Medicine Department of Hepatobiliary and Pancreatic Surgery Department of Liver TransplantationShulan (Hangzhou) Hospital Hangzhou Medical College Zhejiang University School of Medicine

Dear Editor, Liver transplantation(LT) is the only curative therapy for patients with endstage liver disease(He et al., 2023; Ling et al., 2022). Post-transplant hypertriglyceridemia(PTHT), which has an incidence of up to 32.8% in 6 months after LT, is one of the major post-transplant complications(Toshima et al., 2020).

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Reverse modulation of defect density of Ce-MOF-808 by solventassisted ligand exchange for dicyclopentadiene hydrogenation

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Science China Chemistry 2025年

作者： Danfeng Zhao Fajie Hu Rushuo Li Xinmeng xu Zhaokun Wang Zuoshuai Xi jun Tong Xiubing Huang Ge Wang Advanced Innovation Center for Materials Genome Engineering Beijing Key Laboratory of Function Materials for Molecule & Structure Construction School of Materials Science and Engineering University of Science and Technology Beijing

Defect engineering is a pivotal strategy for tailoring the electronic structure and physicochemical properties of catalysts, yet the straightforward manipulation of defect density in porous materials presents a current challenge. In this work, we achieved precise control over the coordination unsaturated site（CUS） content and pore architecture of Ce-MOF-808 by employing 2-fluorobenzoic acid（2-FBA） as a modifier. Leveraging the p Kadisparities among three ligands（1,3,5-tricarboxylic benzoic acid,formic acid, and 2-FBA）, we preserved the spn topology of Ce-MOF within an aqueous system, while realizing partial substitution of the auxiliary ligands and saturation of the CUS. The amount of 2-FBA was negatively correlated with the number of ligand defects, and the consequent decrease in Ce3+content and the increase in the number of ligand linkages also induced crystal structure transformation. The modulation of the defect structure was correlated with the catalytic performance of dicyclopentadiene（DCPD） hydrogenation reaction, in which Ce-MMF0.15, constructed by the modulation of 0.15 mol amount of 2-fluorobenzoic acid, exhibited an optimal ligand defect density and micro-mesoporous structure, enabling almost complete conversion of DCPD at 100 ℃ and 2 MPa. This article also elucidates the correlation between the regulation of defect density and the accessibility of frustrated Lewis pair（FLP） active sites, guiding the application of novel porous intrinsic FLP materials in hydrogenation catalysis.

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Targeted migration of engrafted neural precursor cells derived from ES cells and their differentiation in A β-injuried hippocampus of rats

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Targeted migration of engrafted neural precursor cells deriv...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： jun TANG,Hai-wei xu~*,Xiao-Tang FAN,Zhi-Fang LI,Da-Bing LI,Li YANG,Guang-Ji ZHOU Department of Physiology,Third Military Medical University,Chongqing,400038,China

＜正＞Neural stem cells（NSCs） could be applied for neuronal regeneration and structural brain repair only when they migrate within the CNS and reach the precise regions where neuronal replacement is *** the brain was injuried, NSCs in the hippocampus and subventricular zone（SVZ） of the adult changed their migratory route and moved toward the injury *** present study,we observed the migration and differentiation of neural precursor cells（NPCs） derived from murine embryonic stem（ES） cells when transplanted into the A β-injuried hippocampus of *** between migration distance and differentiation ratio of the engrafted NPCs was also *** ES cell line MESPU35,which expressing enhanced green fluorescent protein（EGFP）,was differentiated into nestin-positive NPCs with modified serumfree methods before *** migration distance was increased about 5 times 12 weeks *** differentiation ratio to GFAP-positive cells of migratory NPCs kept rising from about 30.41±1.45%in 4 weeks to 49.25±1.23%in 16 weeks,and differentiation ratio to NF200-positive cells was increased from 16.68±0.95%in 4 weeks to 27.94±1.21%in 16 ***,the percentage of migratory GFAP-positive cells distributed at the ipsilateral of Aβplaques were increased from 60.2%to 81.3%,and the percentage of migratory NF200-positive cells localized at the ipsilateral was increased from 61.3%to 84.1%.It showed that there was a significant positive linear correlation between migration distance and the neural differentiation ratio（correlation coefficient,r=0.991）,a significant positive linear correlation between migration distance and differentiation ratio to astrocyte（correlation coefficient,r = 0.953）.Our findings suggested that engrafted NPCs migrated targetedly to the A β-injured site and differentiated into neurons and astrocyte.

关键词： Embryonic stem cells β-amyloid peptide Cell transplantation Differentiation Migration Rat

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大跨网架屋盖结构风振响应的模拟分析

武汉理工大学学报 2006年第7期28卷 67-70页

作者：卫军徐明鸣张华华中科技大学土木工程与力学学院武汉430074

根据空间风场模拟技术和结构随机振动理论,运用MATLAB编制了大跨网架屋盖上各点风速模拟程序,结合ANSYS动力分析模块和APDL参数化设计语言的二次开发,对体育中心网架钢屋盖进行了风振响应的时程模拟分析。结果表明,该方法能合理而准确... 详细信息

根据空间风场模拟技术和结构随机振动理论,运用MATLAB编制了大跨网架屋盖上各点风速模拟程序,结合ANSYS动力分析模块和APDL参数化设计语言的二次开发,对体育中心网架钢屋盖进行了风振响应的时程模拟分析。结果表明,该方法能合理而准确地反映大跨网架屋盖结构在风场作用下的响应,可用于工程设计。

关键词：风场模拟随机振动耦合时程分析

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曲面急变流水面线及床面压力的计算

四川大学学报（工程科学版） 2002年第6期34卷 43-46页

作者：田忠王韦刘善均许唯临四川大学高速水力学国家重点实验室四川成都610065

给出了一个实用、有效的曲面急变流水面线及床面压力的计算方法。经理论分析 ,充分考虑了曲率、水流重力等因素的影响 ,建立了水流的连续方程和动量积分方程 ,应用四阶龙格库塔求解 ,得出其水深、压强和流速值。计算结果表明 ,计算值与... 详细信息

给出了一个实用、有效的曲面急变流水面线及床面压力的计算方法。经理论分析 ,充分考虑了曲率、水流重力等因素的影响 ,建立了水流的连续方程和动量积分方程 ,应用四阶龙格库塔求解 ,得出其水深、压强和流速值。计算结果表明 ,计算值与实测值吻合良好。

关键词：水面线床面压力曲面急变流计算方法曲率水流重力水力学

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Engineering N3C vacancies in hierarchical porous carbon nitride nanosheets for room temperature ultradeep photocatalytic aerobic oxidative desulfurization

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Science China Chemistry 2025年

作者： Yina Nie weijie Yang Xin Wang Xiang Li Lele wei Xiaolin Bai Lin Liu Feng Fu jun Wan College of Chemistry & Chemical Engineering Research Institute of Comprehensive Energy Industrial Technology Shaanxi Key Laboratory of Chemical Reaction Engineering Yan'an University Anhui Engineering Research Center of Carbon Neutrality College of Chemistry and Materials Science Anhui Normal University

The production of ultra-low sulfur clean fuels holds global significance for improving energy efficiency and reducing environmental pollution. Photocatalytic aerobic oxidative desulfurization（PODS） offers an attractive approach to efficiently remove refractory heterocyclic aromatic sulfur compounds under mild conditions. However, the sluggish charge dynamics,insufficient molecule activation, and slow mass transfer within photocatalysts restrict the overall desulfurization efficiency,hindering the widespread application of this technology. Herein, we developed a defective g-C3N4（M1U3CN） photocatalyst with bridged N3Cvacancies and hierarchical porous structures to address these limitations. This catalyst not only demonstrates outstanding dibenzothiophene（DBT） removal efficiency of 99.6% for model oil with high conversion and selectivity but also achieves complete sulfur removal from gasoline as well as 96.7% sulfur removal for distilled diesel at room temperature,successfully achieving ultradeep aerobic oxidative desulfurization. The introduction of rich N3Cvacancies and abundant micromeso-macroporous distribution in M1U3CN enhances kinetic efficiencies by promoting charge carrier separation, mass transfer,and molecular activation simultaneously; resulting in exceptional PODS performance. This work provides a collaborative strategy to overcome kinetic challenges in aerobic oxidative desulfurization via defect and structural engineering of the catalyst.

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Synthesis of chiral phosphine derivatives through a copper（I）-catalyzed desymmetric SNAr reaction

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Science China Chemistry 2025年

作者： Shuai Zhang Pei-Zhi Liu Yan-Bo Li Qi Zhang jun-Zhao Xiao Yi-Fan Wang Tian-Yu wei Jia-wei Jiang Ye Wang Qian Peng Liang Yin Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences State Key Laboratory of Elemento-Organic Chemistry and Tianjin Key Laboratory of Biosensing and Molecular Recognition College of Chemistry Frontiers Science Center for New Organic Matter Nankai University Haihe Laboratory of Sustainable Chemical Transformations

Axially chiral phosphines are a class of extremely important ligands in asymmetric catalysis. Herein, an unprecedented copper（I）-catalyzed asymmetric SNAr reaction with HP（S）R2is uncovered, which delivers a series of axially chiral phosphine derivatives in high yields with excellent enantioselectivity. Furthermore, the diastereoselective reaction with the kinetic resolution of racemic HP（S）ArR proceeds smoothly, providing the products with moderate diastereoselectivity and excellent enantioselectivity. Control experiments indicate that HP（S）Ph2is much more active than HPPh2and HP（O）Ph2as the pronucleophile in the present reaction. It is found that the SNAr reaction （the first SNAr） proceeds in a desymmetric manner, providing the desired chiral products while a continued SNAr reaction （the second SNAr） slightly occurs in a kinetic resolution manner in the present catalytic system, which enhances the ee of the desired chiral products. DFTcalculations support a concerted reaction pathway rather than a step-wise mechanism. Finally, axially chiral olefin-phosphines produced by the present method work as suitable ligands in Pdcatalyzed asymmetric allylic substitution with C-, N-, and O-nucleophiles and Rh-catalyzed asymmetric addition of arylboronic acid to benzil.

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Ni-catalyzed cross-electrophile alkyl-alkyl coupling reactions

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Science China Chemistry 2025年

作者： Li-xu You Lan Tian Chun-Ling Guo Shun-Xi Li Yu-Cheng Liu Yu-Long Li wei Shu College of Chemistry and Environmental Engineering Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan Sichuan University of Science and Engineering Guangming Advanced Research Institute Shenzhen Grubbs Institute Department of Chemistry and Guangdong Provincial Key Laboratory of Catalysis Southern University of Science and Technology

Introducing saturated carbon-carbon bonds into organic molecules significantly increases the three-dimensional configuration,thus altering the physical and chemical properties as well as the functions of molecules. Transition-metal-catalyzed carboncarbon cross-couplings represent one of the most straightforward, efficient and modular alternatives to build saturation in a single step. However, the efficient construction of alkyl-alkyl bonds remains a formidable challenge due to the precise manipulation of multiple alkyl metallic intermediates as well as associated competitive side reactions and selectivity control. Over the past years, Ni-catalyzed cross-coupling under reductive conditions has gained more and more attention for alkyl-alkyl crosscoupling partially attributing to the low-valent state of metal centers under reducing conditions, which suppresses the competitive β-hydride elimination process, thus facilitating the formation of alkyl-alkyl bonds. In this review, the initiative and progress of Ni-catalyzed cross-electrophile alkyl-alkyl cross-coupling, including the efforts in racemic and enantioselective formation of alkyl-alkyl bonds, have been summarized and discussed. Moreover, the challenges and further opportunities in this area have also been discussed.

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