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Development and application of an efficient virus-induced gene silencing system in Nicotiana tabacum using geminivirus alphasatellite

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第2期12卷 83-92页

作者： Chang-jun huang tong ZHANG Fang-fang LI Xin-yue ZHANG Xue-ping ZHOU State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310029 China

Virus-induced gene silencing (VIGS) is a recently developed technique for characterizing the function of plant genes by gene transcript suppression and is increasingly used to generate transient loss-of-function assays. Here we report that the 2mDNA1, a geminivirus satellite vector, can induce efficient gene silencing in Nicotiana tabacum with Tobacco curly shoot virus. We have successfully silenced the β-glucuronidase (GUS) gene in GUS transgenic N. tabacum plants and the sulphur desaturase (Su) gene in five different N. tabacum cultivars. These pronounced and severe silencing phenotypes are persistent and ubiquitous. Once initiated in seedlings, the silencing phenotype lasted for the entire life span of the plants and silencing could be induced in a variety of tissues and organs including leaf, shoot, stem, root, and flower, and achieved at any growth stage. This system works well between 18-32°C. We also silenced the NtEDS1 gene and demonstrated that NtEDS1 is essential for N gene mediated resistance against Tobacco mosaic virus in N. tabacum. The above results indicate that this system has great potential as a versatile VIGS system for routine functional analysis of genes in N. tabacum.

关键词： Virus induced gene silencing Geminivirus Alphasatellite Nicotiana tabacum

General Automatic Solution Generation for Social Problems

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Machine Intelligence Research 2025年第1期22卷 145-159页

作者： tong Niu Haoyu huang Yu Du Weihao Zhang Luping Shi Rong Zhao Center for Brain-Inspired Computing Research(CBICR) Optical Memory National Engineering Research Centerand Department of Precision InstrumentTsinghua UniversityBeijing100084China IDG/McGovern Institute for Brain Research Tsinghua UniversityBeijing100084China THU-CET HIK Joint Research Center for Brain-Inspired Computing Beijing100084China

Given the escalating intricacy and multifaceted nature of contemporary social systems,manually generating solutions to address pertinent social issues has become a formidable *** response to this challenge,the rapid development of artificial intelligence has spurred the exploration of computational methodologies aimed at automatically generating ***,current methods for the auto-generation of solutions mainly concentrate on local social regulations that pertain to specific ***,we report an automatic social operating system(ASOS)designed for general social solution generation built upon agent-based models that enables both global and local analyses and regulations of social problems across spatial and temporal *** adopts a hypergraph with extensible social semantics for a comprehensive and structured representation of social *** also incorporates a generalized protocol for standardized hypergraph operations and a symbolic hybrid framework that delivers interpretable solutions,yielding a balance between regulatory efficacy and functional *** demonstrate the effectiveness of the ASOS,we apply it to the domain of averting extreme events within international oil futures *** generating a new trading role supplemented by new mechanisms,ASOS can adeptly discern precarious market conditions and make front-running interventions for nonprofit *** study demonstrated that ASOS provides an efficient and systematic approach for generating solutions for enhancing our society.

关键词： General automatic generation global-local regulation hypergraph social representation symbolic hybrid scheme generalized operation protocol

“No-donor” liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 23-28页

作者： Yong-Fa huang Zhi-jun Zhu Liver Transplantation Center National Clinical Research Center for Digestive DiseasesBeijing Friendship HospitalCapital Medical UniversityBeijing 101100China Clinical Center for Pediatric Liver Transplantation Capital Medical UniversityBeijing 101100China

Liver transplantation is hindered by organ shortage. The potential way to relieve this issue is to expand the donor pool via extending the donor criteria and make full use of all available grafts. The concept of “no-donor” liver transplantation allows grafts to be recovered from other liver recipients. This review summarizes the current clinical practice of “no-donor” liver transplantation, focusing on the experiences of Chinese transplant teams. Domino liver transplantation was introduced by Furtado in 1995 and implemented later in 2013 in China, and novel donor indications including some essential-to-treat inherited metabolic liver-based diseases have emerged. The concept of cross-auxiliary domino liver transplantation brings a further expansion of the domino liver graft pool, and the first pair of liver transplantation performed “rigorously without donation” was accomplished in our center in 2018. Our experience with this original transplantation procedure is hereby reviewed. In order to further promote and make successful “no-donor” liver transplantation, close co-operation between researchers, surgeons, physicians, organ procurement organizations, as well as ethical committees is required.

关键词： Domino liver transplantation Cross-auxiliary domino liver transplantation Inherited metabolic liver-based disease

Hierarchical V_(3)S_(4)/C nanofibers with fast kinetics for superior alkali metal batteries

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Rare Metals 2024年第4期43卷 1836-1844页

作者： Li Chen Zhi-Xin Rao Zhi Chen Zhao-Hui Wu Shuai Yang Zhi-jun Feng Hui-Yong Yang Fan-Yan Zeng Xi-Bao Li Jin-Yang Hu jun-tong huang School of Materials Science and Engineering Nanchang Hangkong UniversityNanchang330063China School of Aeronautical Manufacturing Engineering Nanchang Hangkong UniversityNanchang330063China

Owing to the numerous benefits,including low cost and great theoretical capacity,V3S4 has been a research hotspot for Li^(+)/Na^(+)/K^(+) ***,the V_(3)S_(4) electrode with long cycling stability and high specific capacities continues to pose significant *** this work,vacuum vulcanization is combined with electrospinning to synthesize V_(3)S_(4)/C composite nanofibers(V_(3)S_(4)@CNF).

关键词： composite fibers kinetics

Reverse modulation of defect density of Ce-MOF-808 by solventassisted ligand exchange for dicyclopentadiene hydrogenation

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Science China Chemistry 2025年

作者： Danfeng Zhao Fajie Hu Rushuo Li Xinmeng Xu Zhaokun Wang Zuoshuai Xi jun tong Xiubing huang Ge Wang Advanced Innovation Center for Materials Genome Engineering Beijing Key Laboratory of Function Materials for Molecule & Structure Construction School of Materials Science and Engineering University of Science and Technology Beijing

Defect engineering is a pivotal strategy for tailoring the electronic structure and physicochemical properties of catalysts, yet the straightforward manipulation of defect density in porous materials presents a current challenge. In this work, we achieved precise control over the coordination unsaturated site（CUS） content and pore architecture of Ce-MOF-808 by employing 2-fluorobenzoic acid（2-FBA） as a modifier. Leveraging the p Kadisparities among three ligands（1,3,5-tricarboxylic benzoic acid,formic acid, and 2-FBA）, we preserved the spn topology of Ce-MOF within an aqueous system, while realizing partial substitution of the auxiliary ligands and saturation of the CUS. The amount of 2-FBA was negatively correlated with the number of ligand defects, and the consequent decrease in Ce3+content and the increase in the number of ligand linkages also induced crystal structure transformation. The modulation of the defect structure was correlated with the catalytic performance of dicyclopentadiene（DCPD） hydrogenation reaction, in which Ce-MMF0.15, constructed by the modulation of 0.15 mol amount of 2-fluorobenzoic acid, exhibited an optimal ligand defect density and micro-mesoporous structure, enabling almost complete conversion of DCPD at 100 ℃ and 2 MPa. This article also elucidates the correlation between the regulation of defect density and the accessibility of frustrated Lewis pair（FLP） active sites, guiding the application of novel porous intrinsic FLP materials in hydrogenation catalysis.

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Experimental Study and a Modified Model for Temperature-Recovery Stress of Shape Memory Alloy Wire under Different Temperatures

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Structural Durability & Health Monitoring 2025年第2期19卷 347-364页

作者： Zhi-Xiang Wei Wen-Wei Wang Yan-Jie Xue Wu-tong ZhangQiu-Di huang Guangxi Lepu Expressway Co. Ltd.Nanning530000China Department of Bridge Engineering School of TransportationSoutheast UniversityNanjing211189China Department of Road and Bridge Engineering College of Construction EngineeringJilin UniversityChangchun130012China

To investigate the performance of utilizing the shape memory effect of SMA(Shape Memory Alloy)wire to generate recovery stress,this paper performed single heating recovery stress tests and reciprocating heating-cooling recovery stress tests on SMA wire under varying initial strain *** effects of various strains and different energized heating methods on the recovery stress of SMA wires were explored in the single heating *** SMA wire was strained from 2%to 8%initially,and two distinct heating approaches were employed:one using a large current interval for rapid heating and one using a small current interval for slower *** experimental outcomes reveal that during a single heating cycle,the temperature-recovery stress relationship of SMA wire exhibits three distinct stages:the martensite phase stage,the transition stage from martensite to austenite phase,and the austenite phase ***,the choice of heating method does not influence the maximum recovery stress value,and the correlation between initial strain and maximum recovery stress is predominantly ***,conducting the reciprocating temperature rise and fall performance test is important to better simulate the scenario in practical engineering where multiple recovery stress in SMA wires for structural *** this test,two temperature cycling methods were studied:interval rise and fall,as well as direct rise and *** the case of utilizing the interval temperature rise and fall method,it was observed that the recovery stress associated with cooling was significantly higher than that corresponding to heating at the same ***,the recovery stress was lower upon subsequent heating than that measured during the previous heating *** on the experimental results,a prediction model for the temperature-recovery stress relationship has been proposed to simplify numerical *** is hoped that an approximate temperaturerecovery stress curve can be

关键词： SMA wire temperature recovery stress modified model phase transition

Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO2 nano-membrane for enhancing catalytic performance

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Chinese Chemical Letters 2025年第2期36卷 279-283页

作者： Guang-Xu Duan Queting Chen Rui-Rui Shao Hui-huang Sun tong Yuan Dong-Hao Zhang College of Pharmaceutical Science Hebei University Affiliated Hospital of Hebei University Key Laboratory of Pharmaceutical Quality Control of Hebei Province College of Pharmaceutical ScienceHebei University Institute of Life Science and Green Development Hebei University

The preparation of immobilized enzyme with excellent performance is one of the difficulties that restrict the application of enzyme catalysis technology. Here, Candida rugosa lipase（CRL） was firstly adsorbed on the surface of magnetic zeolitic imidazolate framework-8（ZIF-8） nanospheres, which was further encapsulated with a mesoporous SiO2nano-membrane formed by tetraethyl orthosilicate（TEOS） polycondensation. Consequently, lipase could be firmly immobilized on carrier surface by physical binding rather than chemical binding, which did not damage the active conformation of enzyme. There were mesopores on the silica nano-membrane, which could improve the accessibility of enzyme and its apparent catalytic activity. Moreover, silica membrane encapsulation could also improve the stability of enzyme, suggesting an effective enzyme immobilization strategy. It showed that TEOS amount and the encapsulation time had significant effects on the thickness of silica membrane and the enzyme activity. The analysis in enzyme activity and protein secondary structure showed that lipase encapsulated in silica membrane retained the active conformation to the greatest extent. Compared with the adsorbed lipase, the encapsulated lipase increased its thermostability by 3 times and resistance to chemical denaturants by 7 times. The relative enzyme activity remained around 80% after 8 repetitions, while the adsorbed lipase only remained at7.3%.

关键词： Encapsulation Enzyme immobilization Lipase activity Mesoporous silica membrane ZIF-8

Effect of h-BN rods on high-temperature properties of alumina-carbon refractory

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Journal of Iron and Steel Research International 2025年第1期32卷 115-128页

作者： Ya-ge Li Shuai-bing Wu Feng Liang Zhong huang Tu Long Long Dong jun-yi Lv Hong-juan Duan Biao Wen Yong Liu Hai-jun Zhang The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan430081HubeiChina Sinosteel Luoyang Institute of Refractories Research Co. Ltd.Luoyang471039HenanChina

h-BN rods modified low-carbon alumina-carbon(Al_(2)O_(3)-C)refractories were prepared,and the effect of h-BN rod addition on the high-temperature properties was investigated and compared with commercial h-BN flake,carbon black,and carbon nanotubes *** results demonstrated that Al_(2)O_(3)-C refractories containing h-BN rods exhibited optimal high-temperature service performances,including 25%higher hot modulus of rupture,21.3%higher thermal shock strength residual ratio,20.9%lower in oxidation and 44.3%less in slag corrosion,compared to the counterpart specimens without ***,benefiting from the synergistic enhancement of the rod-like h-BN and in-situ generated SiC whiskers,the high-temperature service performances of h-BN rods containing specimens outperformed counterpart specimens containing commercial h-BN flake,carbon black,and carbon nanotubes,respectively.

关键词： Al_(2)O_(3)-C refractory h-BN rod Microstructure High-temperature service performance

Modified Pulsatilla decoction alleviates 5-fluorouracil-induced intestinal mucositis by modulating the TLR4/MyD88/NF-κB pathway and gut microbiota

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World Journal of Gastroenterology 2025年第7期31卷 110-129页

作者： Yi-tong Qiu Xin-Yi Luo Ya-Feng Deng Xue Zheng Jian-Guo Qiu Lin-Sheng Zhang Xiao-Qi huang Xue-Bao Zheng Hai-Yang huang School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 525000Guangdong ProvinceChina Druggability Research Team Dongguan Institute of Guangzhou University of Chinese MedicineDongguan 523808Guangdong ProvinceChina Institute of Traditional Chinese Medicine Dongguan Hospital of Traditional Chinese MedicineDongguan 523000Guangdong ProvinceChina

BACKGROUND Modified Pulsatilla decoction(PD),a PD with licorice and ejiao,is a classic Traditional Chinese Medicine formula with significant efficacy in treating intestinal mucositis(IM)induced by tumor ***,its specific molecular and biological mechanisms remain *** To investigate the therapeutic effect and mechanism of modified PD in *** This study used an IM mouse model established using 5-fluorouracil injections to investigate the effects of the modified PD(3,6,and 12 g/kg)in *** primary chemical components of the modified PD were identified using liquid chromatography-mass *** weight loss,diarrhea scores,intestinal length,histopathological scores,and inflammatory cytokine levels were measured to evaluate the effects of the modified PD in *** on the TLR4/MyD88/NF-κB pathway were evaluated using western blot *** intestinal microbiota was characterized using Illumina NovaSeq *** The results showed that modified PD significantly improved weight loss and diarrhea and shortened the intestines in IM ***,modified PD suppressed the TLR4/MyD88/NF-κB pathway and downregulated the expression of reactive oxygen species,lipopolysaccharides,and pro-inflammatory cytokines(IL-1β,TNF-α,IFN-γ,IL-6,IL-8,and IL-17),while increasing the expression of the anti-inflammatory cytokine ***,modified PD protected the intestinal mucosal barrier by increasing the expression of tight junction proteins(occludin-1,claudin-1,and ZO-1)and ***,16S rDNA sequencing revealed that modified PD improved intestinal *** Our research offers new insights into the potential mechanism of modified PD in alleviating IM and provides experimental evidence supporting its pharmaceutical application in clinical IM treatment.

关键词： Modified Pulsatilla decoction Intestinal mucositis Oxidative stress Gut microbiota,TLR4/MyD88/NF-κB pathway Inflammatory response