检索结果-南通市图书馆

Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCR

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第3期18卷 268-274页

作者： Zhou-Rui Tang Kai Li Yu-Xun Zhou Zhen-Xian xiao jun-hua xiao Rui huang Guo-Hao Gu College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shang hai 200237 China Department of Pre-Clinical Medicine School Soochow University Suzhou 215123 Jiangsu Prov ince China

AIM: To establish a multiple detection method based on comparative polymerase chain reaction (cPCR) and ligase detection reaction (LDR)/ligase chain reaction (LCR) to quantify the intestinal bacterial components. METHODS: Comparative quantification of 16S rDNAs from different intestinal bacterial components was used to quantify multiple intestinal bacteria. The 16S rDNAs of different bacteria were amplified simultaneously by cPCR. The LDR/LCR was examined to actualize the genotyping and quantification. Two beneficial (Bifidobacterium , Lactobacillus ) and three conditionally pathogenic bacteria (Enterococcus , Enterobacterium and Eubacterium ) were used in this detection. With cloned standard bacterial 16S rDNAs, standard curves were prepared to validate the quantitative relations between the ratio of original concentrations of two templates and the ratio ofthe fluorescence signals of their final ligation products. The internal controls were added to monitor the whole detection flow. The quantity ratio between two bacteria was tested. RESULTS: cPCR and LDR revealed obvious linear correlations with standard DNAs, but cPCR and LCR did not. In the sample test, the distributions of the quantity ratio between each two bacterial species were obtained. There were significant differences among these distributions in the total samples. But these distributions of quantity ratio of each two bacteria remained stable among groups divided by age or sex. CONCLUSION: The detection method in this study can be used to conduct multiple intestinal bacteria genotyping and quantification, and to monitor the human intestinal health status as well.

关键词： 16s rDNA Comparative quantification Com- parative polymerase chain reaction Intestinal bacteria Ligase chain reaction Ligase detection reaction

The denitrogenation by breathing oxygen-rich gas to prevent altitude decompression sickness

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国应用生理学杂志 2012年第6期28卷 568-571页

作者： hua-jun xiao xiao-peng LIU Bin ZANG Gui-you WANG Zhao GU Institute of Aviation Medicine

Objective While the technology of the molecular sieve oxygen generation system(MSOGS) onboard was used,pilots could not breathe pure oxygen to eliminate nitrogen during a high altitude *** is no report whether it is a threat to altitude decompression sickness(ADS) or not in that *** experiment was intended to observe the effects of breathing different oxygen-rich gases of MSOGS on denitrogenation,so that we could make the medical physiological requirements for MSOGS on-board and provide experimental basis for aeromedical *** Eight healthy males were breathed oxygenrich gases(60%,70%,80%,90%and 99.6%) in turn for 60 min,and the concentration of nitrogen,oxygen,carbon dioxide and argon at the end of expiration interval in the oxygen mask were continuously measured by a flight mass spectrometer through the oxygen *** to the variety of the denitrogenation rate by breathing different oxygen-rich gases,its change law was *** There were significant differences(P<0.05) about denitrogenation rate in different oxygen-rich gases due to different oxygen concentration and breathing *** denitrogenation rate of pure oxygen was higher than that of the *** was indicated that the concentration of nitrogen in lung would decrease along with the increase in oxygen concentration of oxygen-rich gases,and the nitrogen concentration in the lung almost decreased by 50% or even more if people were breathed 60%～90% oxygen-rich gas longer than 60 *** The man-made respiration environment of low nitrogen can be provided by breathing oxygen-rich gases,although the denitrogenation velocity of breathing oxygen-rich gases is lower than that of breathing pure *** it can be used as a measure to eliminate and lower the nitrogen in the body to prevent from ADS.

关键词：脱氮率呼吸气体富氧减压高空氧气面罩时间间隔

Hydrocarbon Accumulation Condition and Exploration Direction in the Deepwater Area of Qiongdongnan Basin,Northern South China Sea

在线全文

学校读者我要写书评

暂无评论

地学前缘 2009年第S1期16卷 147-148页

作者： jun xiao,hua Wang,hua Jiang Faculty of Earth Resources,China University of Geosciences(Wuhan),Wuhan 430074,China.

The deepwater area of southern Qiongdongnan Basin is a hydrocarbon exploration frontier and mainly located on the continental slope in the northwestern South China *** tectonic and depositional evolution is similar to the typical marginal deepwater areas abroad where oils have been *** hydrocarbon conditions in this area are as follows:(1) three sets of source rocks (including lacustrine mudstone of Eocene,coastal plain coal-bearing strata and semi-closed shallow sea mudstone of Oligocene,and marine mudstone

关键词： Qiongdongnan Basin deepwater area condition of hydrocarbon reservoir forming hydrocarbon exploration

MicroRNA-155 promotes the pathogenesis of experimental colitis by repressing SHIP-1 expression

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第6期23卷 976-985页

作者： Zhan-jun Lu Jian-Jiong Wu Wei-Liang Jiang jun-hua xiao Kai-Zhong Tao Lei Ma Ping Zheng Rong Wan Xing-Peng Wang Department of Gastroenterology Shanghai General HospitalShanghai Jiao Tong University School of Medicine Department of Gastroenterology Shanghai East HospitalSchool of MedicineTong Ji University

AIM To explore the mechanism by which microRNA-155 (miR-155) regulates the pathogenesis of experimental colitis. METHODS A luciferase assay was performed to confirm the binding of miR-155 to the SHIP-1 3'-UTR. MiR-155 mimics, negative controls and SHIP-1 expression/knockdown vectors were established and then utilized in gain-and loss-of-function studies performed in raw264.7 cells and primary bone marrow-derived macrophages (BMDMs). Thereafter, dextran sulfate sodium (DSS)-induced colitis mouse model with or without antagomiR-155 treatment was established, and the levels of miR-155 and SHIP-1, as well as the pro-inflammatory capabilities, were measured by western blot, quantitative polymerase chain reaction, and immunohistochemistry. RESULTS MiR-155 directly bound to the 3'-UTR of SHIP-1 mRNA and induced a significant decrease in SHIP-1 expression in both raw264.7 cells and primary BMDMs. MiR-155 markedly promoted cell proliferation and proinflammatory secretions including IL-6, TNF-alpha, IL-1 beta, and IFN-gamma, whereas these effects could be reversed by the restoration of SHIP-1 expression. In vivo studies showed that antagomiR-155 administration could alleviate DSS-induced intestinal inflammation in Balb/c mice. Moreover, significantly increased SHIP-1 expression, as well as decreased Akt activation and in-flammatory response, were observed in the antagomiR-155-treated mice. CONCLUSION MiR-155 promotes experimental colitis by repressing SHIP-1 expression. Thus, the inhibition of miR-155 might be a promising strategy for therapy.

关键词： Experimental colitis Inflammatory bowel disease MicroRNA-155 SHIP-1

Synthesis of Pyrrole-Imidazole Polyamide

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2000年第3期11卷 207-208页

作者： jun hua xiao Gu YUAN Wei Qiang huaNG Fei Li TANG Albert S.C.CHAN K.L.Daniel LEE Bioorganic Molecular Engineering Laboratory Department of ChemistryPeking UniversityBeijing 100871 Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHK

One simple and versatile method is established far the synthesis of DNA recognition molecules-polyamides containing alternating N-methylpyrrole and N-methylimidazole without necessitating NH2- group protection.

关键词： polyamides DNA recognition molecules pyrroles and imidazoles

Optical Antenna System Design for 3D Imaging Lidar

在线全文

学校读者我要写书评

暂无评论

Journal of Electronic Science and Technology 2011年第1期9卷 67-70页

作者： hua-jun Yang xiao-Ping huang the School of Physical Electronics University of Electronic Science and Technology of China Chengdu 610054 China

A new aspheric surface pre-collimation lenses system for the optical antenna of three-dimensional （3D） imaging of lidar has been optimally designed and simulated by optical design software *** kinds of aspheric surfaces spherical lenses including the sections of spherical,elliptical,hyperbola,and parabola have been *** optical system,including the elliptical cylinder lenses collimation and the optical antenna,can be realized less than 5 rad collimation angle for dot source semiconductor laser beam.

关键词： Aspheric surface pre-collimation lenses system optical antenna three-dimensional imaging lidar.

A novel autoantibody in patients with primary hypertension： antibody against L-type Ca^2＋ channel

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2008年第16期121卷 1513-1517页

作者： ZHOU Zi-hua WANG jun xiao hua CHEN Zhi-jian WANG Min CHENG Xiang LIAO Yu-hua Institute of Cardiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China

Background Recently, it has been proposed that the autoantibodies against various cardiovascular receptors play a role in the pathogenesis of primary hypertension. In this study, we aimed to identify whether or not there are autoantibodies against cardiovascular L-type Ca^2＋ channels in patients with primary hypertension. Methods A peptide corresponding to the sequence 2-16 of the alc-subunit of L-type Ca^2＋ channel was used as an antigen to screen the autoantibodies from 90 patients with primary hypertension and 45 healthy controls by an enzyme-linked immunosorbent assay （ELISA）. The clinical data of 90 hypertensive patients were compared between patients with and without these autoantibodies. Results Serum from 3 （6.7%） of the 45 healthy controls, 33 （36.7%） of 90 hypertensives showed positive responses in ELISA （P 〈0.01）. The prevalence of such autoantibodies in two subgroups of hypertensives with coronary heart disease （9/21, 57.14%, P 〈0.05） and left ventricular diastolic dysfunction （28/63, 44.4%, P 〈0.05） was higher than in those without the corresponding complications. And the patients with such autoantibodies had lower E/A than patients without such autoantibodies （0.803±0.191 vs 1.004±0.322, P=0.002）. Conclusion There are autoantibodies against vascular L-tyPe Ca^2＋ channels in patients with primary hvnertension.

关键词： autoantibody primary hypertension L-trpe Ca^2+ channel

Angiotensin H Induces Mesenchymal Stem Cells Proliferation in vitro

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药理通讯 2008年第2期25卷 39-40页

作者： xiao-jun Wang xiao-hua Zhong Rui-Zhen Shi Ji-Chang Wang Song-hua huang Qing-Ping Li Department of Pharmacology Nanjing Medical University Nanjing 210029

lncRNA SNHG4 enhanced gastric cancer progression by modulating miR-409-3p/CREB1 axis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2025年第1期33卷 185-198页

作者： ZHOUYANG CHENG YUCHEN hua YANG CAO jun QIN Department of General Surgery Affiliated Hospital of Nantong UniversityNantong226001China Department of Surgery Affiliated Hospital of Nantong UniversityMedical School of Nantong UniversityNantong226001China Department of Operation Affiliated Hospital of Nantong UniversityNantong226001China

Objective:Gastric cancer(GC)is a globally common cancer characterized by high incidence and mortality *** in the molecular understanding of GC provide promising targets for GC diagnosis and *** non-coding RNAs(lncRNAs)and their downstream regulators are regarded to be implicated in the progression of multiple types of *** have shown that the lncRNA small nucleolar RNA host gene 4(SNHG4)serves as a tumor promoter in various malignancies,while its function in GC has yet to be ***,our study aimed to explore the role and underlying mechanism of SNHG4 in ***:We used qRT-PCR to analyze SNHG4 expression in GC tissues and ***-Meier analysis was used to assess the correlation between SNHG4 expression and the survival rate of GC *** function experiments such as CCK-8,BrdU,colony formation,flow cytometry analysis,and transwell were performed to explore the effects of SNHG4 on GC cell proliferation,apoptosis,cell cycle,migration,and *** also established xenograft mouse models to explore the effect of SNHG4 on GC tumor ***,dual luciferase reporter assay was used to verify the interaction between SNHG4 and miR-409-3p and between miR-409-3p and cAMP responsive element binding protein 1(CREB1).Results:The results indicated that SNHG4 was overexpressed in GC tissues and cell lines,and was linked with poor survival rate of GC ***4 promoted GC cell proliferation,migration,and invasion while inhibiting cell apoptosis and cell cycle arrest in *** in vivo experiment indicated that SNHG4 facilitated GC tumor ***,SNHG4 was demonstrated to bind to ***,CREB1 was directly targeted by *** assays demonstrated that miR-409-3p deficiency reversed the suppressive impact of SNHG4 knockdown on GC cell ***,miR-409-3p was also revealed to inhibit GC cell proliferation,migration,and invasion by targeting CREB1

关键词： Gastric cancer Small nucleolar RNA host gene 4(SNHG4) MicroRNA-409-3p(miR-409-3p) cAMP responsive element binding protein 1(CREB1)