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Enhanced tolerance to drought in transgenic rice plants overexpressing C_4 photosynthesis enzymes

The Crop Journal 2013年第2期1卷 105-114页

作者： jun-fei gu Ming Qiu Jian-Chang Yang Key Laboratory of Crop Genetics and Physiology of Jiangsu Province College of AgricultureYangzhou University

Maize-specific pyruvate orthophosphate dikinase(PPDK) was overexpressed in rice independently or in combination with the maize C4-specific phosphoenolpyruvate carboxylase(PCK). The wild-type(WT) cultivar Kitaake and transgenic plants were evaluated in independent field and tank experiments. Three soil moisture treatments,well-watered(WW), moderate drought(MD) and severe drought(SD), were imposed from 9d post-anthesis till maturity. Leaf physiological and biochemical traits, root activities,biomass, grain yield, and yield components in the untransformed WT and two transgenic rice lines(PPDK and PCK) were systematically studied. Compared with the WT, both transgenic rice lines showed increased leaf photosynthetic rate: by 20%–40% under WW, by45%–60% under MD, and by 80%–120% under SD. The transgenic plants produced 16.1%,20.2% and 20.0% higher grain yields than WT under the WW, MD and SD treatments,respectively. Under the same soil moisture treatments, activities of phosphoenolpyruvate carboxylase(PEPC) and carbonic anhydrase(CA) in transgenic plants were 3–5-fold higher than those in WT plants. Compared with ribulose-1,5-bisphosphate carboxylase, activities of PEPC and CA were less reduced under both MD and SD treatments. The transgenic plants also showed higher leaf water content, stomatal conductance, transpiration efficiency, and root oxidation activity and a stronger active oxygen scavenging system than the WT under all soil moisture treatments, especially MD and SD. The results suggest that drought tolerance is greatly enhanced in transgenic rice plants overexpressing C4photosynthesis enzymes. This study was performed under natural conditions and normal planting density to evaluate yield advantages on a field basis. It may open a new avenue to droughttolerance breeding via overexpression of C4enzymes in rice.

关键词： Pyruvate orthophosphate dikinase(PPDK) C4-specific phosphoenolpyruvate carboxylase(PEPC) and PPDK(PCK) Transgenic rice Photosynthesis Drought tolerance

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Nanostructured bimetallic Zn/Co in N-doped carbon as an efficient catalyst for the alcohol dehydrogenation to carboxylic acids under solvent-free conditions

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Journal of Materials Science & Technology 2023年第30期161卷 111-122页

作者： Zhong-Hui Zheng Somboon Chaemchuen jun-fei gu Jing Hang Wei Sang Ji-Chao Wang Ye Yuan Cheng Chen State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China

The catalytic alcohol dehydrogenation to carboxylic acids has been demonstrated as an atom-economic and environmental-friendly synthetic ***,the reported catalysts either require relatively complicated synthesis or exhibit high activity only in the presence of a ***,it is still in high demand to obtain catalysts that feature facile synthesis and perform well even without any ***,a series of nanostructured bimetallic Zn/Co species embedded in N-doped hierarchical porous carbon were fabricated in a facile and green *** significantly,the best-performing material(Zn/Co@NC-800)was identified as the first heterogeneous catalyst to enable this transformation under solvent-free ***,the as-prepared composites were comprehensively investigated to de-termine their structural and morphological properties,which rationalized the superiority of Zn/Co@NC-800 over the other synthesized *** studies signified that Co sites(both Co^(0)and Co^(2+))and N species(mainly pyridinic N)were probably involved in this process,functioning as the Lewis acid site and basic site,***,this study could provide a simple and sustainable method for carboxylic acid synthesis.

关键词： Heterogeneous catalysis Bimetallic ZIFs Pyrolysis Solvent-free Alcohol dehydrogenation Carboxylic acids

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Research progress on the effect of pyroptosis on the occurrence,development,invasion and metastasis of colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3410-3427页

作者： Xu Wang Qi-Hang Yin Lin-Lu Wan Ruo-Lan Sun Gang Wang jun-fei gu De-Cai Tang School of Traditional Chinese Medicine Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina Department of Ana and Intestine Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina

Pyroptosis is a type of programmed cell death mediated by gasdermines(GSDMs).The N-terminal domain of GSDMs forms pores in the plasma membrane,causing cell membrane rupture and the release of cell contents,leading to an inflammatory response and mediating *** plays an important role in inflammatory diseases and malignant *** the further study of pyroptosis,an increasing number of studies have shown that the pyroptosis pathway can regulate the tumor microenvironment and antitumor immunity of colorectal cancer and is closely related to the occurrence,development,treatment and prognosis of colorectal *** review aimed to explore the molecular mechanism of pyroptosis and the role of pyroptosis in the occurrence,development,treatment and prognosis of colorectal cancer(CRC)and to provide ideas for the clinical diagnosis and treatment of CRC.

关键词： Cell pyroptosis Tumor metastasis Colorectal cancer Clinical diagnosis Review

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First observation results of Macao Science Satellite-1 on lightning-induced electron precipitation

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Science China Earth Sciences 2025年第1期68卷 1-10页

作者： Yongping WANG Yixin SUN Qiugang ZONG Gaopeng LU Xudong gu Ze-jun HU Bin LI Key Laboratory for Polar Science Ministry of Natural Resources Polar Research Institute of China Institute of Space Physics and Applied Technology Peking University State Key Laboratory of Lunar and Planetary Science Macau University of Science and Technology School of Earth and Space Sciences University of Science and Technology of China School of Electronic Information Wuhan University

The Medium-energy Electron Spectrometers(MES) is a space-borne instrument onboard Macao Science Satellite-1(MSS-1) dedicated to monitoring the typical charged particle radiation characteristics in the satellite orbit and the process of their occurrence and development, including short bursts of lightning-induced electron precipitation(LEP).This paper presents the first results of the LEP measurements by the MSS-1.47 LEP events are identified with the routine operation for 3 months since satellite launch, all within the range of 1.5

关键词： Lightning-induced electron precipitation Inner radiation belts Energetic electrons Macao Science Satellite-1

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Integrated data mining and network pharmacology to discover a novel traditional Chinese medicine prescription against diabetic retinopathy and reveal its mechanism

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TMR Modern Herbal Medicine 2024年第2期7卷 41-55页

作者： Kai-Lun Zhang Xu Wang Xiang-Wei Chang jun-fei gu Bo-Yang Zhu Shi-Bing Wei Bo Wu Can Peng Jiu-Sheng Nie De-Ling Wu College of Pharmacy Anhui University of Chinese MedicineHefei 230012China School of Traditional Chinese Medicine and School of Integrated Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing 210023China MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials Anhui University of Chinese MedicineHefei 230012China Institute of Pharmaceutics Anhui Academy of Chinese MedicineHefei 230012China Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application Anhui University of Chinese MedicineHefei 230012China Engineering Technology Research Center of Modernized Pharmaceutics Anhui Education Department(AUCM)Hefei 230012China The First Clinical Medical College Anhui University of Chinese MedicineHefei 230012China

Background:Diabetic retinopathy(DR)is currently the leading cause of blindness in elderly individuals with *** Chinese medicine(TCM)prescriptions have shown remarkable effectiveness for treating *** study aimed to screen a novel TCM prescription against DR from patents and elucidate its medication rule and molecular mechanism using data mining,network pharmacology,molecular docking and molecular dynamics(MD)***:TCM prescriptions for treating DR was collected from patents and a novel TCM prescription was identified using data ***,the mechanism of the novel TCM prescription against DR was explored by constructing a network of core TCMs-core active ingredients-core targets-core ***,molecular docking and MD simulation were employed to validate the findings from network ***:The TCMs of the collected prescriptions primarily possessed bitter and cold properties with heat-clearing and supplementing effects,attributed to the liver,lung and kidney ***,a novel TCM prescription for treating DR was identified,composed of Lycii Fructus,Chrysanthemi Flos,Astragali Radix and Angelicae Sinensis *** core active ingredients and ten core targets of the novel TCM prescription for treating DR were ***,the novel TCM prescription played a crucial role for treating DR by inhibiting inflammatory response,oxidative stress,retinal pigment epithelium cell apoptosis and retinal neovascularization through various pathways,such as the AGE-RAGE signaling pathway in diabetic complications and the MAPK signaling ***,molecular docking and MD simulation demonstrated that almost all core active ingredients exhibited satisfactory binding energies to core ***:This study identified a novel TCM prescription and unveiled its multi-component,multi-target and multi-pathway characteristics for treating *** findings provide a scientific basis and novel insights into

关键词： TCM prescriptions diabetic retinopathy medication rule molecular mechanism data mining network pharmacology molecular docking

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Evaluation of combined therapy with transarterial chemoembolization and percutaneous ethanol injection for large hepatocellular carcinoma

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当代医学(中国介入放射学) 2008年第3期 280-284页

作者： FAN Wei-jun,GAO fei,gu Yang-kui,HUANG Jin-hua,LI You-he,LI Wen-quan,LU Lian-wei Weijun Fan,Gao fei,gu Yangkui,Jinhua Huang,Wenquan Li,Department of Imaging and Interventional Radiology,Cancer Center,Sun Yat-sen University,China,510060

Objective To assess the effects of combined transcatheter arterial chemoembolization(TACE) and percutaneous ethanol injection(PEI) in patients with large hepatocellular carcinoma(HCC).Methods A total of 63 patients with large unresectable HCC was treated with TACE followed by *** largest dimension of the tumours ranged from 5.3 cm to 17.8 *** rates,acute effects,toxicity and prognostic factors were ***-up ranged from 3 months to 62 *** The cumulative survival rates at 1,3 and 5 years were 59.4%,28.4% and 15.8% respectively(median survival is 27.7 months).A reduction of tumor area greater than 50%,was achieved in 48.6% of *** 86.9% of the cases with increased a-feto protein(AFP) values,AFP level underwent a reduction of more than 75%.The combination therapy was generally well *** two patient died from variceal bleeding associated with *** Cox proportional hazards model showed that the number of tumours the tumor margin and the ethanol dose were independent prognostic *** The results indicate that combined TACE with PEI is a promising therapeutic approach for large unresectable HCC.

关键词： Hepatocellular carcinoma Chemoembolization Ethanol injection Combination therapy

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Development of an improved CBR model for predicting steel temperature in ladle furnace refining

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International Journal of Minerals,Metallurgy and Materials 2021年第8期28卷 1321-1331页

作者： fei Yuan An-jun Xu Mao-qiang gu School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

In the prediction of the end-point molten steel temperature of the ladle furnace, the influence of some factors is nonlinear. The prediction accuracy will be affected by directly inputting these nonlinear factors into the data-driven model. To solve this problem, an improved case-based reasoning model based on heat transfer calculation(CBR-HTC) was established through the nonlinear processing of these factors with software Ansys. The results showed that the CBR-HTC model improves the prediction accuracy of end-point molten steel temperature by5.33% and 7.00% compared with the original CBR model and 6.66% and 5.33% compared with the back propagation neural network(BPNN)model in the ranges of [-3, 3] and [-7, 7], respectively. It was found that the mean absolute error(MAE) and root-mean-square error(RMSE)values of the CBR-HTC model are also lower. It was verified that the prediction accuracy of the data-driven model can be improved by combining the mechanism model with the data-driven model.

关键词： case-based reasoning LF refining steel temperature prediction ladle lining

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan jun-Bo Yang Zhi-Li Zuo Han guo Zi-fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei gu Xin Shao Qinqin Wang Xin Dong Bin Duan feiyang Xing jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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电切镜联合输尿管镜在腺性膀胱炎中寻找困难输尿管开口的应用

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中国内镜杂志 2017年第2期23卷 73-76页

作者：谷军飞任立新张勇王柱李建兴赵清河北医科大学第二医院泌尿外科河北石家庄050000

目的探讨电切镜联合输尿管镜在腺性膀胱炎中寻找困难输尿管开口的方法,提高内镜下置入双J管的成功率。方法回顾性分析2015年3月-2016年5月,8例腺性膀胱炎合并有困难输尿管开口的患者的临床资料。均为男性患者,年龄38~64岁,平均44.3岁。... 详细信息

目的探讨电切镜联合输尿管镜在腺性膀胱炎中寻找困难输尿管开口的方法,提高内镜下置入双J管的成功率。方法回顾性分析2015年3月-2016年5月,8例腺性膀胱炎合并有困难输尿管开口的患者的临床资料。均为男性患者,年龄38~64岁,平均44.3岁。电切膀胱内病变,切除深度为黏膜下层和肌层,将输尿管开口区域的坏死组织切除干净,切除深度能够显露肌层的纹理改变。进一步切除肌肉组织,透过薄层肌肉组织能够看见尿液。在导丝引导下置入输尿管镜,镜下观察证实为输尿管管腔。在导丝引导下留置双J管一根。结果 8例患者(16侧)均应用电切镜联合输尿管镜成功置入双J管。手术时间35~205 min,平均时间83.2 min,术中失血量约20~50 ml。术后3~5天拔除尿管。术后1或2天复查腹部平片,显示双侧双J管位置良好,没有出现异位、扭曲等现象。围手术期未出现并发症。结论应用电切镜联合输尿管镜在腺性膀胱炎中寻找困难输尿管开口,能够提高寻找输尿管开口成功的概率,此方法安全、创伤小、避免开放手术。

关键词：腺性膀胱炎输尿管开口双J管电切镜输尿管镜检查术

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