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Effects of early rehabilitation in improvement of paediatric burnt hands function

World Journal of Clinical Cases 2021年第32期9卷 9741-9751页

作者： Ya-Qin zhou jun-yi zhou Gao-Xing Luo Jiang-Lin Tan Institute of Burn Research State Key Laboratory of TraumaBurns and Combined InjuriesChongqing Key Laboratory for Disease ProteomicsSouthwest HospitalThe Third Military Medical University(Army Medical University)Chongqing 400038China

BACKGROUND Hands are one of the most common burn sites in *** scar contractures in hands after wound healing result in further reductions in their range of motion(ROM),motility,and fine motor *** can improve the function of *** the optimal time of rehabilitation intervention is still ***,this study was designed to investigate the effects of early rehabilitation management of paediatric burnt hands and to compare the efficacy between early and later rehabilitation *** To investigate the effects of early rehabilitation management of paediatric burnt *** A total of 52 children with burnt hands were allocated into the early intervention group(≤1 mo from onset)and a late intervention group(>1 mo from onset)between January 2016 and December *** children received the same rehabilitation programme including skin care,scar massage,passive ROM exercises,active ROM exercises,compression therapy,orthotic devices wearing and game or music *** assessments were performed before and after the rehabilitation *** In the early intervention group,the ROM of the hands was significantly improved after rehabilitation(P=0.001).But in the late group the effect was not significant statistically(P=0.142).In the early group,38.5%of the patients showed significant improvement,while in the late group,69.2%of the patients showed no significant *** time from onset to posttraumatic rehabilitation(P=0.0007)and length of hospital stay(P=0.003)were negatively correlated with the hand function *** length of rehabilitation stay was positively correlated with the hand function improvement(P=0.005).CONCLUSION These findings suggest that early rehabilitation might show better results in terms of ROM.

关键词： Burns Children Rehabilitation Range of motion Hand flexion Hand extension

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Linoleic Acid Activates GPR40/FFA1 and Phospholipase C to Increase [Ca^(2+)]_i Release and Insulin Secretion in Islet Beta-Cells

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Chinese Medical Sciences Journal 2012年第1期27卷 18-23页

作者： yi-jun zhou Yu-ling Song Hui zhou Yan Li Department of Endocrinology and Metabolism Fourth Affiliated HospitalChina Medical UniversityShenyang 110034China

Objective To elucidate GPR40/FFA1 and its downstream signaling pathways in regulating insulin secretion. Methods GPR40/FFA1 expression was detected by immunofluorescence imaging. We employed linoleic acid (LA), a free fatty acid that has a high affinity to the rat GPR40, and examined its effect on cytosolic free calcium concentration ([Ca2+]i) in primary rat β-cells by Fluo-3 intensity under confocal microscopy recording. Downregulation of GPR40/FFA1 expression by antisense oligonucleotides was performed in pancreatic β-cells, and insulin secretion was assessed by enzyme-linked immunosorbent assay. Results LA acutely stimulated insulin secretion from primary cultured rat pancreatic islets. LA induced significant increase of [Ca2+]i in the presence of 5.6 mmol/L and 11.1 mmol/L glucose, which was reflected by increased Fluo-3 intensity under confocal microscopy recording. LA-stimulated increase in [Ca2+]i and insulin secretion were blocked by inhibition of GPR40/FFA1 expression in β-cells after GPR40/FFA1-specific antisense treatment. In addition, the inhibition of phospholipase C (PLC) activity by U73122, PLC inhibitor, also markedly inhibited the LA-induced [Ca2+]i increase. Conclusion LA activates GPR40/FFA1 and PLC to stimulate Ca2+ release, resulting in an increase in [Ca2+]i and insulin secretion in rat islet β-cells.

关键词： GPR40 / FFA 1 phospholipase C antisense oligonucleotides intracellular calcium linoleic acid

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Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells

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Chinese Medical Sciences Journal 2009年第4期24卷 231-235页

作者： Jia-he Wang yi-jun zhoux yi-jun zhou Li Tian Ping He Department of Geriatrics Shengjing Hospital China Medical University Shenyang 110004 China Department of Endocrinology and Metabolism the Fourth Affiliated Hospital China Medical University Shenyang 110032 China

Objective To explore the mechanisms involved in Staphylococcus aureus （S. aureus） invading human monocytic U937 cells. Methods S. aureus were added to U937 cells at multiplicity of infections （MOI） of 20：1 for 0, 15, 30, 60, and 90 minutes, respectively. Cell apoptosis was analyzed with Hoechst 33258 staining and Annexin V-fluorescein isothiocyanate （FITC）/propidium iodide （PI） flow cytometry analysis. Akt and nuclear factor-κB （NF-κB） activities were detected by Western blotting. Results Infection of U937 cells with S. aureus induced rapid cell death in a time-dependent manner, and the cells displayed characteristic features of apoptosis. S. aureus-induced apoptosis was associated with a prominent downregulation of activated （phosphorylated） Akt and NF-κB. The inhibition of phosphorylated Akt by LY294002 led to the inhibition of NF-κB in a dose-dependent manner. Inhibition of Akt with LY294002 caused further increase in apoptosis of U937 cells. Conclusions S. aureus can stimulate the apoptosis of U937 ceils. S. aureus induces apoptosis of U937 cells by inhibiting Akt-regulated NF-κB.

关键词： Staphylococcus aureus apoptosis U937 cells phosphoinositide 3-kinase nuclear factor-κB

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HEPATOCYTE GROWTH FACTOR PROTECTS AGAINST APOPTOSIS INDUCED BY ADVANCED GLYCATION END PRODUCTS IN ENDOTHELIAL CELLS

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Chinese Medical Sciences Journal 2006年第1期21卷 6-10页

作者： yi-jun zhou Jia-he Wang Jin Zhang Department of Endocrinology and Metabolism China Medical University Shenyang 110001 Department of Infectious Diseases the First Affiliated Hospital China Medical University Shenyang 110001

Objective To investigate the effects of hepatocyte growth factor(HGF)on vascular endothelial cells apoptosis induced by advanced glycation end products(AGEs)and its possible mechanism. Methods Human umbilical vein endothelial cells(HUVECs)were cultured in vitro and intervened by different concentrations of AGEs and *** cell inhibitory rates of each group with different culture time(12, 24, 48, and 72 hours)were measured by methyl thiazolyl tetrazolium(MTT)assay. The early stage apoptosis was detected by flow cytometry with Annexin V-FITC/PI double staining, morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the expression of apoptosis-associated genes Bax and Bcl-2 were determined by Western *** activity of caspase-3 was detected by enzyme-linked immunosorbent assay (ELISA).Results Morphological observation indicated that high concentration of AGEs induced characteristic apoptotic changes in *** a certain concentration range, HUVECs apoptosis inducing rates by AGEs were in both dose- and time-dependent *** significantly inhibited the apoptosis of HUVECs induced by AGEs (P< 0.05).AGEs significantly promoted expression of Bax protein, but not *** HGF significantly promoted the expression of Bcl-2(P<0.01)and decreased the activity of caspase-3(P<0.05)without affecting Bax *** AGEs can induce the apoptosis of endothelial cells in *** may effectively attenuate AGEs-induced endothelial cells apoptosis through upregulating Bcl-2 gene expression and inhibiting caspase-3 activation.

关键词： hepatocyte growth factor advanced glycation end products vascular endothelial cell apoptosis atherosclerosis

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Nucleotide-binding Oligomerization Domain-1 Ligand Induces Inflammation and Attenuates Glucose Uptake in Human Adipocytes

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Chinese Medical Sciences Journal 2012年第3期27卷 147-152页

作者： yi-jun zhou Ai Li Yu-ling Song Yan Li Hui zhou Department of Endocrinology and Metabolism Fourth Affiliated Hospital of China Medical UniversityShenyang 110032China

Objective To investigate the effects of stimulant for nucleotide-binding oligomerization domain 1 （NOD1） on secretion of proinflammatory chemokine/cytokines and insulin-dependent glucose uptake in human differentiated adipocytes. Methods Adipose tissues were obtained from patients undergoing liposuction. Stromal vascular cells were extracted and differentiated into adipocytes. A specific ligand for NOD1, was administered to human adipocytes in culture. Nuclear factor-κB transcriptional activity and proinflammatory chemokine/cytokines production were determined by reporter plasmid assay and enzyme-linked immunosorbent assay, respectively. Insulin-stimulated glucose uptake was measured by 2-deoxy-D-[ 3 H] glucose uptake assay. Furthermore, chemokine/cytokine secretion and glucose uptake in adipocytes transfected with small interfering RNA （siRNA） targeting NOD1 upon stimulation of NOD1 ligand were analyzed. Results Nuclear factor-κB transcriptional activity and monocyte chemoattractant protein-1 （MCP-1）, interleukin （IL）-6, and IL-8 secretion in human adipocytes were markedly increased stimulated with NOD1 ligand （all P〈0.01）. Insulin-induced glucose uptake was decreased upon the activation of NOD1 （P〈0.05）. NOD1 gene silencing by siRNA reduced NOD1 ligand-induced MCP-1, IL-6, and IL-8 release and increased insulin-induced glucose uptake （all P〈0.05）. Conclusion NOD1 activation in adipocytes might be implicated in the onset of insulin resistance.

关键词： nucleotide-binding oligomerization domain-1 human adipocyte inflammation insulin resistance

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INVOLVEMENT OF p38 MITOGEN-ACTIVATED PROTEIN KINASE IN ***-INDUCED U937 APOPTOSIS

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Chinese Medical Sciences Journal 2007年第1期22卷 49-53页

作者： Jia-he Wang yi-jun zhou Ping He Bai-yi Chen Department of Geriatrics the Second Affiliated Hospital China Medical University Shenyang 110004 Department of Endocrinology and Metabolism the Fourth Affiliated Hospital China Medical University Shenyang 110032 Department of Infectious Diseases the First Affiliated Hospital China Medical University Shenyang 110001

Objective To investigate whether the effect of E. coli on U937 cell fines apoptosis is mediated via p38 mitogen-activated protein kinase （MAPK） activation. Methods The U937 cell lines were treated with E. coli at different time or together with SB203580, an inhibitor for p38. Cell apoptosis was analyzed by flow cytometry, p38 activities were detected by Western blotting. Results E. coli induced apoptosis in cultured U937 cell lines in a time-dependent manner. The phosphorylation of p38 was induced after 10 minutes infection, reached the peak after 20 minutes, and started to decline after 30 minutes. In contrast, the level of total p38 protein was not changed in whole experimental period. Inhibition of p38 with SB203580 significantly inhibited E. coli induced apoptosis in U937 cells. Conclusion The activation of the p38 MAPK in U937 cell lines by E. coli is a major pathway to mediate the apoptosis.

关键词： E. coli apoptosis U937 p38 mitogen-activated protein kinase

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Saturated Fatty Acid Induces Insulin Resistance Partially Through Nucleotide-binding Oligomerization Domain 1 Signaling Pathway in Adipocytes

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Chinese Medical Sciences Journal 2013年第4期28卷 211-217页

作者： yi-jun zhou yin-si Tang Yu-ling Song Ai Li Hui zhou Yan Li Department of Endocrinology and Metabolism Fourth Affiliated Hospital of China Medical University

Objective To investigate the potential role of nucleotide-binding oligomerization domain 1 （NOD1）, a component of the innate immune system, in mediating lipid-induced insulin resistance in adipocytes. Methods Adipocytes from Toll-like receptor 4 deficiency mice were used for stimulation experiments. The effect of oleate/palmitate mixture on nuclear factor-κB （NF-κB） activation was analyzed by reporter plasmid assay. The release of proinflammatory chemokine/cytokines production was determined by using real-time PCR. Insulin-stimulated glucose uptake was measured by 2-deoxy-D-[SH] glucose uptake assay. Chemokine/cytokine expression and glucose uptake in adipocytes transfected with small interfering RNA （siRNA） targeting NOD 1 upon fatty acids treatment were analyzed. Results Oleate/palmitate mixture activated the NF-κB pathway and induced interleukin-6, tumor necrosis factor-R, and monocyte chemoattractant protein-1 mRNA expressions in adipocytes from mice deficient in Toll-like receptor 4, and these effects were blocked by siRNA targeting NOD1. Furthermore, saturated fatty acids decreased the ability of insulin-stimulated glucose uptake. Importantly, siRNA targeting NOD 1 partially reversed saturated fatty acid-induced suppression of insulin-induced glucose uptake. Conclusion NOD1 might play an important role in saturated fatty acid-induced insulin resistance in adipocytes, suggesting a mechanism by which reduced NOD1 activity confers beneficial effects on insulin action.

关键词： nucleotide-binding oligomerization domain 1 saturated fatty acids innate immunity insulin resistance

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Clinical outcomes of robotic-assisted and manual total hip arthroplasty in the same patient:A case report

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World Journal of Clinical Cases 2023年第23期11卷 5519-5524页

作者： Tian-Ye Hu Dao-Chao Lin yi-jun zhou Zhi-Wu Zhang Jia-jun Yuan Department of Orthopaedics Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhou 311000Zhejiang ProvinceChina

BACKGROUND Total hip arthroplasty(THA)is an effective treatment for advanced osteonecrosis of the femoral head,which can significantly relieve pain and improve patients'quality of ***-assisted THA enhances the accuracy and stability of THA surgery and achieves better clinical outcomes than manual *** SUMMARY We report the clinical outcomes of robotic-assisted THA and manual THA in the same patient with osteonecrosis of the femoral head.A 49-year-old male patient attended our hospital due to more than 3 years of pain in both hip *** left hip was treated with robotic-assisted *** patient underwent manual THA of the right hip 3 mo after robotic-assisted *** obtained postoperative radiograph parameters,Harris hip score and forgotten joint score of the patient 1 year after *** Compared with manual THA,the patient’s left hip felt better 1 year after roboticassisted ***-assisted THA resulted in a better Harris hip score and forgotten joint score than manual THA in the same patient with osteonecrosis of the femoral head.

关键词： Total hip arthroplasty Robotic-assisted Osteonecrosis of the femoral head Case report

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Syngas production in a novel perovskite membrane reactor with co-feed of CO_2

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Chinese Chemical Letters 2011年第12期22卷 1492-1496页

作者： Yan ying Wei Liu Huang jun Tang Ling yi zhou Zhong Li Hai Hui Wang School of Chemistry ＆ Chemical Engineering South China University of Technology Guangzhou 510640 China

Partial oxidation of methane（POM） co-fed with CO_2 to syngas in a novel catalytic BaCo_（0.6）Fe_（0.2）Ta_（0.2）O_（3-δ） oxygen permeable membrane reactor was successfully *** CO_2 to the partial oxidation of methane reaction not only alters the ratio of CO/H_2,but also increases the oxygen permeation flux and CH_4 *** 96%CH_4 conversion with more than 93%CO_2 conversion and 100%CO selectivity is achieved,which shows an excellent reaction performance.A steady oxygen permeation flux of 15 mL/（cm^2 min） is obtained during the 100-h operation,which shows good stability as well.

关键词： POM Oxygen separation CO_2 Perovskite Membrane reactor

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Morphology and ontogeny of dendritic cells in rats at different development periods

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World Journal of Gastroenterology 2009年第10期15卷 1246-1253页

作者： yi-jun zhou Juan Gao Hua-Mei Yang Jian-Xin Zhu Tong-Xin Chen Zhen-Juan He Xinhua Hospital Affiliated to Medical College Shanghai Jiaotong University Shanghai 200092 China Shanghai Sixth People's Hospital Affiliated toMedical College Shanghai Jiaotong University Shanghai200233 China Xinhua Hospital Affiliated to Medical College Shanghai Jiaotong University Shanghai 200092 China

AIM： To study the morphology and ontogeny of dendritic cells of Peyer＇s patches in rats at different development periods. METHODS： The morphometric and flow cytometric analyses were performed to detect all the parameters of villous-crypts axis and the number of OX62＋DC, OX62＋CD4＋SIRP＋DC, and OX62＋CD4-SIRP-DC in the small intestine in different groups of rats. The relationship between the parameters of villous-axis and the number of DC and DC subtype were analyzed. RESULTS： All morphometric parameters changed significantly with the development of pups in the different age groups （F = 10.751, 12.374, 16.527, 5.291, 3.486; P = 0.000, 0.000, 0.000, 0.001, 0.015）. Villous height levels were unstable and increased from 115.24μm to 140.43 μm as early as 3 wk postpartum. Villous area increased significantly between 5 and 7 wk postpartum, peeked up to 13817.60 tam2 at 7 wk postpartum. Villous height and crypt depth ratios were relatively stable and increased significantly from 2.80 ＋ 1.01 to 4.54 =1= 1.56, 9-11 wk postpartum. The expression of OX62＋DC increased from 33.30%±5.80% to 80%± 17.30%, 3-11 wk postpartum （F =5.536, P = 0.0013）. OX62＋CD4＋SIRP＋DC subset levels detected in single-cell suspensions of rat total Peyer＇s patch dendritic cells （PP-DCs） increased significantly from 30.73% ± 5.16% to 35.50% ± 4.08%, 5-7 wk postpartum and from 34.20% ±1.35% to 43.60% ± 2.07% 9-11 wk postpartum （F = 7.216, P = 0.005）. CONCLUSION： This study confirms the agerelated changes in villous-crypt axis differentiation in the small intestine. Simultaneously, there are also development and maturation in rat PP-DCs phenotypic expression. Furthermore, the morphological changes of intestinal mucosa and the development of immune cells （especially DC） peaked at 9-11 wk postpartum, indicating that the intestinal mucosae reached a relatively mature state at 11 wk postpartum.

关键词： Intestinal mucosa Dendric cell Peyer'spatches Intestinal development

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