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Chinese Chemical Letters 2016年第6期27卷 813-816页

作者： Xue-Mei Li Rui-Rui Zhao Yu-Ling Wei Dan Yang zhang-Jian Zhou jun-feng zhang Ying Zhou Department of Safety Evaluation Technology Center of China Tobacco Yunnan Industrial Co. Ltd. College of Chemical Science and Technology Yunnan University College of Chemistry and Chemical Engineering Yunnan Normal University

A rhodamine-based sensor has been developed for the detection of mercuric ions. The colorimetric and fluorescence responses, allowing naked-eye detections, are based on Hg^2＋-induced opening of the rhodamine spirocycle. Among all the testes ions, only Hg^2＋generated a significant fluorescence enhancement of up to 300-fold, with a bright yellow–green emission. This sensor was a low toxic compound, and was successfully applied in the in vivo imaging of Hg^2＋in Spill 2 cells and C. elegans. This approach provides a sensitive and accurate method for the estimation of Hg^2＋in environmental, tobacco and biological applications.

关键词： Chemsensor Mercury Rhodamine Colorimetric Fluorescence In vivo imaging

The genomic,transcriptomic,and immunological profiles of perineural invasion in pancreatic ductal adenocarcinoma

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Science China(Life Sciences) 2023年第1期66卷 183-186页

作者： Shu-Heng Jiang Rong-Kun Li De-jun Liu jun-Li Xue Min-Hao Yu Shan zhang Li-Min Liu jun-feng zhang Rong Hua Yong-Wei Sun Xu Wang Qin Yang Zhi-Gang zhang State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteRen Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200240China Institute of Oncology Affiliated Hospital of Jiangsu UniversityZhenjiang 212001China Department of Biliary-Pancreatic Surgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200217China Department of Oncology Shanghai East HospitalTongji University School of MedicineShanghai 200123China Department of Gastrointestinal Surgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200217China Department of Oral Pathology Shanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineShanghai 200011China State Key Laboratory of Oncogenes and Related Genes Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Shanghai Institute of Precision Medicine Shanghai 200125China

Dear Editor,Cancer neuroscience is an emerging topic in cancer research(Monje et al.,2020;Shi et al.,2022).Perineural invasion(PNI),defined as the neoplastic invasion of tumor cells into or surrounding the different layers of nervous fibers(epineural,perineural,and endoneural spaces of the neuronal sheath),is associated with aggressive tumor behavior.

关键词： invasion neural

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Gamma-aminobutyric acid enhances miR-21-5p loading into adipose-derived stem cell extracellular vesicles to alleviate myocardial ischemia-reperfusion injury via TXNIP regulation

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World Journal of Stem Cells 2024年第10期16卷 873-895页

作者： feng-Dan Wang Yi Ding Jian-Hong Zhou En Zhou Tian-Tian zhang Yu-Qi Fan Qing He Zong-Qi zhang Cheng-Yu Mao jun-feng zhang Jing Zhou Department of Cardiology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Department of Anesthesiology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

BACKGROUND Myocardial ischemia-reperfusion injury(MIRI)poses a prevalent challenge in current reperfusion therapies,with an absence of efficacious interventions to address the underlying *** To investigate whether the extracellular vesicles(EVs)secreted by adipose mesenchymal stem cells(ADSCs)derived from subcutaneous inguinal adipose tissue(IAT)underγ-aminobutyric acid(GABA)induction(GABA-EVs^(IAT))demonstrate a more pronounced inhibitory effect on mitochondrial oxidative stress and elucidate the underlying *** We investigated the potential protective effects of EVs derived from mouse ADSCs pretreated with *** assessed cardiomyocyte injury using terminal deoxynucleotidyl transferase dUTP nick end-labeling and Annexin V/propidium iodide *** integrity of cardiomyocyte mitochondria morphology was assessed using electron microscopy across various intervention *** explore the functional RNA diversity between EVs^(IAT)and GABA-EVs^(IAT),we employed microRNA(miR)*** a dual-luciferase reporter assay,we confirmed the molecular mechanism by which EVs mediate thioredoxin-interacting protein(TXNIP).Western blotting and immunofluorescence were conducted to determine how TXNIP is involved in mediation of oxidative stress and mitochondrial *** Our study demonstrates that,under the influence of GABA,ADSCs exhibit an increased capacity to encapsulate a higher abundance of miR-21-5p within ***,this leads to a more pronounced inhibitory effect on mitochondrial oxidative stress compared to EVs from ADSCs without GABA intervention,ultimately resulting in myocardial *** a molecular mechanism level,EVs regulate the expression of TXNIP and mitigating excessive oxidative stress in mitochondria during MIRI process to rescue *** Administration of GABA leads to the specific loading of miR-21-5p into EVs by ADSCs,thereby regulating the expression of *** EVs derived from

关键词： Extracellular vesicles Myocardial ischemia-reperfusion injury Adipose-derived mesenchymal stem cells Gammaaminobutyric acid Thioredoxin-interacting protein

Strategic insights into the cultivation of pancreatic cancer organoids from endoscopic ultrasonography-guided biopsy tissue

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World Journal of Gastroenterology 2024年第42期30卷 4532-4543页

作者： Jia-Li Yang jun-feng zhang Jian-You Gu Mei Gao Ming-You Zheng Shi-Xiang Guo Tao zhang Institute of Hepatopancreatobiliary Surgery Chongqing General HospitalChongqing UniversityChongqing 401147China

BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced *** To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized *** Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively *** refined the endoscopic biopsy procedures and organoid cultivation *** tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic *** assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability *** In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor *** organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five *** these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO *** key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical *** Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultiv

关键词： Endoscopic ultrasound-guided fine-needle biopsy Pancreatic cancer organoid Puncture technique Cultivation optimization strategy Drug screening

Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton

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Journal of Integrative Plant Biology 2009年第5期51卷 456-465页

作者： Hai-Hong Shang Chuan-Liang Liu Chao-jun zhang feng-Lian Li Wei-Dong Hong Fu-Guang Li Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture Chinese Cotton Research Institute Chinese Agricultural Academy of Sciences Anyang 455004 China

Over the past few decades genetic engineering has been applied to improve cotton breeding. Agrobacterium medicated transformation is nowadays widely used as an efficient approach to introduce exogenous genes into cotton for genetically modified organisms. However, it still needs to be improved for better transformation efficiency and higher embryogenic callus induction ratios. To research further the difference of mechanisms for morphogenesis between embryogenic callus and non-embryogenic callus, we carried out a systematical study on the histological and cellular ultrastructure of Agrobacterium transformed calli. Results showed that the embryogenic callus developed nodule-like structures, which were formed by small, tightly packed, hemispherical cells. The surface of some embryogenic callus was covered with a fibrilar-like structure named extracellular matrix. The cells of embryogenic calli had similar morphological characteristics. Organelles of embryogenic callus cells were located near the nucleus, and chloroplasts degraded to proplastid-like structures with some starch grains. In contrast, the non-embryogenic calli were covered by oval or sphere cells or small clusters of cells. It was observed that cells had vacuolation of cytoplasm and plastids with a well organized endomembrane system. This study aims to understand the mechanisms of embryogenic callus morphogenesis and to improve the efficiency of cotton transformation in future.

关键词： cell organization pattern chloroplasts embryogenic callus Gossypiurn hirsutum histological structure transformation ultrastructure

Chemotherapy,transarterial chemoembolization,and nephrectomy combined treated one giant renal cell carcinoma(T3aN1M1)associated with Xp11.2/TFE3:A case report

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World Journal of Clinical Cases 2022年第28期10卷 10180-10185页

作者： Peng Wang Xiao zhang Shuo-Han Shao Fa Wu Fei-Zhou Du jun-feng zhang Zhi-Way Zuo Rui Jiang Department of Radiology The General Hospital of Western Theater CommandChengdu 610083Sichuan ProvinceChina

BACKGROUND Renal cell carcinoma(RCC)with Xp11.2 translocation/TFE3 gene fusion is a rare and distinct subtype of RCC that is classified under tumors with translocation of the microphthalmia-associated transcriptional *** SUMMARY We report an adult case of Xp11.2 translocation advanced RCC with metastasis(T3a N1M1),after targeted treatment,alcohol ablation,and transarterial chemoembolization,who eventually underwent successful surgical *** recurrence or transfer was seen within one year,and the survival period was more than 3 years.A review of the relevant literature was conducted to improve our understanding of the pathogenesis,epidemiology,clinical manifestations,diagnosis,differential diagnosis,treatment,and other aspects of the *** Transarterial chemoembolization and ablation did not achieve the desired tumor reduction in this patient,but had a significant effect on reducing intraoperative bleeding and inhibiting tumor activity.

关键词： Xp11.2/TFE3 Renal Cell Carcinoma TACE Axitinib Zoledronic Acid Targeted therapy Multimodal imaging Case report

Extracellular vesicles from hypoxia-preconditioned mesenchymal stem cells alleviates myocardial injury by targeting thioredoxininteracting protein-mediated hypoxia-inducible factor-1αpathway

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World Journal of Stem Cells 2022年第2期14卷 183-199页

作者： Cheng-Yu Mao Tian-Tian zhang Dong-Jiu Li En Zhou Yu-Qi Fan Qing He Chang-Qian Wang jun-feng zhang Department of Cardiology Shanghai Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200010China

BACKGROUND Extracellular vesicles(EVs)derived from hypoxia-preconditioned(HP)mesenchymal stem cells(MSCs)have better cardioprotective effects against myocardial infarction(MI)in the early stage than EVs isolated from normoxic(NC)-***,the cardioprotective mechanisms of HP-EVs are not fully *** To explore the cardioprotective mechanism of EVs derived from HP *** We evaluated the cardioprotective effects of HP-EVs or NC-EVs from mouse adipose-derived MSCs(ADSCs)following hypoxia in vitro or MI in vivo,in order to improve the survival of cardiomyocytes(CMs)and restore cardiac *** degree of CM apoptosis in each group was assessed by the terminal deoxynucleotidyl transferase dUTP nick end-labeling and Annexin V/PI ***(miRNA)sequencing was used to investigate the functional RNA diversity between HP-EVs and NC-EVs from mouse *** molecular mechanism of EVs in mediating thioredoxin-interacting protein(TXNIP)was verified by the dual-luciferase reporter ***-immunoprecipitation,western blotting,and immunofluorescence were performed to determine if TXNIP is involved in hypoxia-inducible factor-1 alpha(HIF-1α)ubiquitination and degradation via the chromosomal region maintenance-1(CRM-1)-dependent nuclear transport *** HP-EVs derived from MSCs reduced both infarct size(necrosis area)and apoptotic degree to a greater extent than NC-EVs from CMs subjected to hypoxia in vitro and mice with MI in *** of EV-associated miRNAs showed the upregulation of 10 miRNAs predicted to bind TXNIP,an oxidative stress-associated *** showed miRNA224-5p,the most upregulated miRNA in HP-EVs,directly combined the 3’untranslated region of TXNIP and demonstrated its critical protective role against hypoxia-mediated CM *** results demonstrated that MI triggered TXNIP-mediated HIF-1αubiquitination and degradation in the CRM-1-mediated nuclear transport pathway in CMs,which led to aggravated injury and hypoxia t

关键词： Extracellular vesicles Myocardial infarction Mesenchymal stem cells Hypoxia preconditioning Thioredoxin-interacting protein Hypoxia-inducible factor 1 alpha

A Rhodamine-based fluorescent sensor for chromium ions and its application in bioimaging

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Chinese Chemical Letters 2017年第6期28卷 1258-1261页

作者： Xue-Mei Li Rui-Rui Zhao Yan Yang Xue Wei Lv Yu-Ling Wei Rui Tan jun-feng zhang Ying Zhou Department of Safety Evaluation Technology Center of China Tobacco Yunnan Industrial Co.Ltd. College of Chemical Science and Technology Yunnan University College of Chemistry and Chemical Engineering Yunnan Normal University

A rhodamine-based sensor（1） has been developed for the detection of chromium ***-（3＋）-induced opening of the rhodamine spirocycle in sensor（1） led to the distinct colorimetric and fluorescence *** all the tested ions,only Cr-（3＋） generated a significant fluorescence enhancement of up to13-fold,which indicated the high selectivity of ***（1） was successfully applied in the in vivo fluorescence imaging of Cr-（3＋） in *** results provided solid evidences for the future estimation of Cr-（3＋） in environmental applications and tobacco samples.

关键词： Sensor Chromium Rhodamine Colorimetric Fluorescence In vivo imaging

Clinicopathological,treatment,and prognosis study of 43 gastric neuroendocrine carcinomas

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World Journal of Gastroenterology 2017年第3期23卷 516-524页

作者： De-jun Liu Xue-Liang Fu Wei Liu Lu-Ying Zheng jun-feng zhang Yan-Miao Huo Jiao Li Rong Hua Qiang Liu Yong-Wei Sun Department Biliary-Pancreatic Surgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department Pathology Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China

AIM To provide more information and therapeutic methods about gastric neuroendocrine carcinomas(G-NECs) which occur rarely but are highly malignant and clinically *** We retrospectively analyzed the clinicopathological characteristics, treatments, and prognosis of 43 G-NEC patients at our hospital between January 2007 and December 2014. The diagnosis was based on the 2010 World Health Organization *** Forty-three G-NECs containing 39 small cell carcinomas and 4 large cell NECs with Ki67 > 60% were included in this study, accounting for only 0.95% of all gastric carcinomas. The median patient age was 62 years (range, 33-82) and the male-to-female ratio was 4.4:1. All patients underwent surgery, including 38 curative resections and 5 palliative resections. Among these 43 patients, nearly half(48.84%) of these tumors were located in the cardiac region of the stomach, regional lymph node metastasis was found in 31 cases(72.09%), and liver metastasis was found in 6 cases(13.95%). Follow-up information was got for 40 patients. Twentythree die of this disease with a median survival of 31 mo(range 1-90). The 1-year, 2-year, 3-year, and 5-year survival rate was 77.50%, 57.04%, 44.51%, and 35.05%, respectively. Survival was better in patients with tumor located in the cardiac region of the stomach, less than 7 lymph nodes metastasis and no liver metastasis. Five patients did not undergo postoperative chemotherapy, and the median survival time for these patients was 15 mo. For the remaining 34 patients who received postoperative chemotherapy, the median survival time was 44 mo and those received etoposide, cisplatin, and Paclitaxel survived the best. One patient with resected liver metastasis who received postoperative Capecitabine plus Oxaliplatin and Paclitaxel systemic chemotherapy plus octreotide LAR(30 mg intramuscularly, every 4 wk, for 2 years) has survived for 74 mo with no *** G-NECs are mostly nonfunctioning, which lead

关键词： Gastric neuroendocrine carcinomas Liver metastasis Medical treatment Surgery Prognosis

Clinical significance of programmed death 1/programmed death ligand 1 pathway in gastric neuroendocrine carcinomas

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World Journal of Gastroenterology 2019年第14期25卷 1684-1696页

作者： Min-Wei Yang Xue-Liang Fu Yong-Sheng Jiang Xiao-Jing Chen Ling-Ye Tao Jian-Yu Yang Yan-Miao Huo Wei Liu jun-feng zhang Pei-feng Liu Qiang Liu Rong Hua Zhi-Gang zhang Yong-Wei Sun De-jun Liu Department of Biliary-Pancreatic Surgery Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Central Laboratory Ren Ji Hospital School of MedicineShanghai Jiao Tong University Department of Pathology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiao Tong University

BACKGROUND Recently, more and more studies have demonstrated the pivotal role of programmed death 1/programmed death ligand 1(PD-1/PD-L1) pathway in the immune evasion of tumors from the host immune system. However, the role of PD-1/PD-L1 pathway in gastric neuroendocrine carcinomas(G-NECs) remains *** To investigate the expression of PD-1/PD-L1 and role of PD-1/PD-L1 pathway in G-NECs, which occur rarely but are highly malignant and clinically *** We investigated the expression of PD-L1 on tumor cells and PD-1^+, CD8^+, and FOXP3^+ T cell infiltration by immunohistochemistry in 43 resected G-NEC tissue specimens. The copy number alterations of PD-L1 were assessed by *** Most of the G-NECs tumor cells exhibited a near-uniform expression pattern of PD-L1, while some showed a tumor-stromal interface enhanced pattern. Of the 43G-NECs, 21(48.8%) were classified as a high PD-L1 expression group, and the high expression of PD-L1 was associated with poor overall survival(OS). The high expression of PD-L1 was correlated with abundant PD-1^+ tumor infiltrating lymphocytes(TILs) instead of CD8^+ TILs and FOXP3^+ regulatory T cells(Tregs).Our analysis also suggested that the infiltration of CD8^+ TILs tended to be a favorable factor for OS, although the difference did not reach the statistical significance(P = 0.065). Meanwhile, PD-L1 was significantly overexpressed in cases with copy number gain as compared with those *** Our data demonstrated for the first time that high expression of PD-L1 in GNECs is associated with a poor prognosis, while the high expression may be due to the copy number variation of PD-L1 gene or stimulation of TILs. These results provide a basis for the immunotherapy targeting PD-1/PD-L1 pathway in GNECs.

关键词： Programmed death 1 Programmed death ligand 1 Gastric neuroendocrine carcinomas Prognosis Tumor infiltrating lymphocytes