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Effects of soil metal(loid)s pollution on microbial activities and environmental risks in an abandoned chemical smelting site

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Journal of Environmental Sciences 2024年第9期143卷 60-70页

作者： Hao Li jun yao Jianli Liu Ning Min Geoffrey Sunahara Dongyang Men Robert Duran School of Water Resources and Environment Research Center of Environmental Science and EngineeringChina University of Geosciences(Beijing)Beijing 100083China Department of Natural Resource Sciences McGill University21111Lakeshore DriveSte-Anne-de-BellevueQuebecH9X 3V9Canada Equipe Environnement et Microbiologie MELODY groupUniversitéde Pau et des Pays de l’AdourE2S-UPPAIPREM UMR CNRS 5254BP 115564013 Pau CedexFrance

Abandoned chemical smelting sites containing toxic substances can seriously threaten and pose a risk to the surrounding ecological environment.Soil samples were collected from different depths(0 to 13 m)and analyzed for metal(loid)s content and fractionation,as well as microbial activities.The potential ecological risk indices for the different soil depths(ordered from high to low)were:1 m(D-1)>surface(S-0)>5 m(D-5)>13 m(D-13)>9 m(D-9),ranging between 1840.65-13,089.62,and representing extremely high environmental risks,of which Cd(and probably not arsenic)contributed to the highest environmental risk.A modified combined pollution risk index(MCR)combining total content and mobile proportion of metal(loid)s,and relative toxicities,was used to evaluate the degree of contamination and potential environmental risks.For the near-surface samples(S-0 and D-1 layers),the MCR considered that As,Cd,Pb,Sb,and Zn achieved high and alarming degrees of contamination,whereas Fe,Mn,and Ti were negligible or low to moderate pollution degrees.Combined microcalorimetry and enzymatic activity measurements of contaminated soil samples were used to assess the microbial metabolic activity characteristics.Correlation analysis elucidated the relationship between metal(loid)s exchangeable fraction or content and microbial activity characteristics(p<0.05).The microbial metabolic activity in the D-1 layer was low presumably due to heavy metal stress.Enzyme activity indicators and microcalorimetric growth rate(k)measurements were considered sensitive indicators to reflect the soil microbial activities in abandoned chemical smelting sites.

关键词： Abandoned chemical smelting sites Microbial activities Modified combined pollution risk Microcalorimetry Enzymatic activities

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Phase behavior of gas condensate in porous media using real-time computed tomography scanning

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Petroleum Science 2024年第2期21卷 1032-1043页

作者： Wen-Long Jing Lei Zhang Ai-Fen Li jun-Jie Zhong Hai Sun Yong-Fei Yang Yu-Long Cheng jun yao Institute of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao266580ShandongChina

The phase behavior of gas condensate in reservoir formations differs from that in pressure-volume-temperature(PVT)cells because it is influenced by porous media in the reservoir formations.Sandstone was used as a sample to investigate the influence of porous media on the phase behavior of the gas condensate.The pore structure was first analyzed using computed tomography(CT)scanning,digital core technology,and a pore network model.The sandstone core sample was then saturated with gas condensate for the pressure depletion experiment.After each pressure-depletion state was stable,realtime CT scanning was performed on the sample.The scanning results of the sample were reconstructed into three-dimensional grayscale images,and the gas condensate and condensate liquid were segmented based on gray value discrepancy to dynamically characterize the phase behavior of the gas condensate in porous media.Pore network models of the condensate liquid ganglia under different pressures were built to calculate the characteristic parameters,including the average radius,coordination number,and tortuosity,and to analyze the changing mechanism caused by the phase behavior change of the gas condensate.Four types of condensate liquid(clustered,branched,membranous,and droplet ganglia)were then classified by shape factor and Euler number to investigate their morphological changes dynamically and elaborately.The results show that the dew point pressure of the gas condensate in porous media is 12.7 MPa,which is 0.7 MPa higher than 12.0 MPa in PVT cells.The average radius,volume,and coordination number of the condensate liquid ganglia increased when the system pressure was between the dew point pressure(12.7 MPa)and the pressure for the maximum liquid dropout,Pmax(10.0 MPa),and decreased when it was below Pmax.The volume proportion of clustered ganglia was the highest,followed by branched,membranous,and droplet ganglia.This study provides crucial experimental evidence for the phase behavior changing process of gas condensate in porous media d

关键词： Gas condensate Pressure depletion Real-time micro-computed tomography scanning Distribution of condensate liquid

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Dynamic interwell connectivity analysis of multi-layer waterflooding reservoirs based on an improved graph neural network

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Petroleum Science 2024年第2期21卷 1062-1080页

作者： Zhao-Qin Huang Zhao-Xu Wang Hui-Fang Hu Shi-Ming Zhang Yong-Xing Liang Qi Guo jun yao School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Exploration and Development Research Institute Shengli OilfieldSINOPECDongying257015ShandongChina

The analysis of interwell connectivity plays an important role in the formulation of oilfield development plans and the description of residual oil distribution. In fact, sandstone reservoirs in China's onshore oilfields generally have the characteristics of thin and many layers, so multi-layer joint production is usually adopted. It remains a challenge to ensure the accuracy of splitting and dynamic connectivity in each layer of the injection-production wells with limited field data. The three-dimensional well pattern of multi-layer reservoir and the relationship between injection-production wells can be equivalent to a directional heterogeneous graph. In this paper, an improved graph neural network is proposed to construct an interacting process mimics the real interwell flow regularity. In detail, this method is used to split injection and production rates by combining permeability, porosity and effective thickness, and to invert the dynamic connectivity in each layer of the injection-production wells by attention mechanism.Based on the material balance and physical information, the overall connectivity from the injection wells,through the water injection layers to the production layers and the output of final production wells is established. Meanwhile, the change of well pattern caused by perforation, plugging and switching of wells at different times is achieved by updated graph structure in spatial and temporal ways. The effectiveness of the method is verified by a combination of reservoir numerical simulation examples and field example. The method corresponds to the actual situation of the reservoir, has wide adaptability and low cost, has good practical value, and provides a reference for adjusting the injection-production relationship of the reservoir and the development of the remaining oil.

关键词： Graph neural network Dynamic interwell connectivity Production-injection splitting Attention mechanism Multi-layer reservoir

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Cuproptosis mediates copper-induced testicularspermatogenic cell death

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Asian Journal of Andrology 2024年第3期26卷 295-301页

作者： Jing-Yi Zhang Xu-jun Yu jun-jun Li yao Xiao Guang-Sen Li Fang Yang Liang Dong Chengdu University of Traditional Chinese Medicine Chengdu 611137China Chengdu Fifth People’s Hospital The Fifth People’s Hospital of Chengdu University ofTraditional Chinese MedicineChengdu 611130China TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province Hospital of Chengdu University ofTraditional Chinese MedicineChengdu 610072China Department of Surgery The Reproductive and Women-Children HospitalChengdu University of TraditionalChinese MedicineChengdu 610041China

Cuproptosis,a novel mechanism of programmed cell death,has not been fully explored in the context of spermatogenic cells.Thisstudy investigated the potential involvement of cuproptosis in spermatogenic cell death using a mouse model of copper overload.Sixty male Institute of Cancer Research(ICR)mice were randomly divided into four groups that received daily oral gavage withsodium chloride(control)or copper sulfate(CuSO4)at 50 mg kg−1,100 mg kg−1,or 200 mg kg−1,for 42 consecutive days.Micesubjected to copper overload exhibited a disruption in copper homeostasis.Additionally,significant upregulated expression of keycuproptosis factors was accompanied by a significant rise in the rates of testicular tissue cell apoptosis.Immunohistochemicalanalysis revealed the presence of ferredoxin 1(Fdx1)in Sertoli cells,Leydig cells,and spermatogenic cells at various stages oftesticular development,and the Fdx1-positive staining area was significantly increased in copper-overloaded mice.Mitochondrialdysfunction and decreased adenosine triphosphate levels were also observed,further implicating mitochondrial damage undercuproptosis.Further analyses revealed pathological lesions and blood−testis barrier destruction in the testicular tissue,accompaniedby decreased sperm concentration and motility,in copper-overloaded mice.In summary,our results indicate that copper-overloadedmice exhibit copper homeostasis disorder in the testicular tissue and that cuproptosis participates in spermatogenic cell death.These findings provide novel insights into the pathogenic mechanisms underlying spermatogenic cell death and provide initialexperimental evidence for the occurrence of cuproptosis in the testis.

关键词： blood−testis barrier cuproptosis ferredoxin 1 spermatogenic cell death

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Magnetic nanoparticle-modified hollow double-shell SiC@C@FeCo with excellent electromagnetic wave absorption

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Nano Research 2024年第4期17卷 3164-3174页

作者： Guiyu Peng Jintang Zhou Jiaqi Tao Weize Wang jun Liu junru yao Yijie Liu Zhengjun yao College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 211100China Key Laboratory of Material Preparation and Protection for Harsh Environment(Nanjing University of Aeronautics and Astronautics) Ministry of Industry and Information TechnologyNanjing 211100China

Integrated micro and nanostructures,heterogeneous components,defects,and interfaces is the way to develop high-performance microwave absorbing materials.However,there still needs to be more precise experimental routes and effective validation.In this work,by a continuous process of vacuum sintering,hydrothermal,and carbon thermal reduction,magnetic FeCo nanoparticles were successfully embedded on the hollow double-shell mesoporous SiC@C surface,thus solving the challenges of a single component loss mechanism.The hollow double-shell nanostructure introduces air to enhance impedance matching while significantly reducing the density of the material.The extensive defects and heterogeneous grain boundaries effectively enhance the polarization loss capacity.The magnetic loss mechanism introduced by the magnetic particles effectively improves the impedance matching properties of the material.The synergy of these multiple advantages has enabled the SCFC_(2)-8(here SiC@C@FeCo is abbreviated to SCFC,2 represents the initial metal ion content,and 8 represents the hydrothermal time)sample to achieve an adequate absorption bandwidth of 6.09 GHz at 2.0 mm.With a minimum reflection loss of-60.56 dB,the absorption bandwidth can cover the entire C,X,and Ku bands by adjusting the matching thickness(1.3–4.0 mm).This work provides a valuable paradigm for the deeper exploitation of microwave absorption potential and guides the development of other high-performance materials.

关键词： SiC@C@FeCo(SCFC)composites core–shell hollow structure microwave absorption

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Primary biliary cholangitis presenting with granulomatous lung disease misdiagnosed as lung cancer:A case report

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World Journal of Clinical Cases 2024年第2期12卷 354-360页

作者： Shan-Li Feng jun-yao Li Chun-Ling Dong Department of Respiratory and Critical Care Medicine The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina

BACKGROUND There are few cases of pulmonary granulomatous changes secondary to primary biliary cirrhosis(PBC).No case of granulomatous lung disease secondary to PBC misdiagnosed as lung cancer had been reported.CASE SUMMARY A middle-aged woman presented with lung nodules and was misdiagnosed with lung cancer by positron emission tomography/computed tomography.She underwent left lobectomy,and the pathology of the nodules showed granulomatous inflammation,which was then treated with antibiotics.However,a new nodule appeared.Further investigation with lung biopsy and liver serology led to the diagnosis of PBC,and chest computed tomography indicated significant reduction in the pulmonary nodule by treatment with methylprednisolone and ursodeoxycholic acid.CONCLUSION Diagnosis of pulmonary nodules requires integrating various clinical data to avoid unnecessary pulmonary lobectomy.

关键词： Granulomatous lung diseases Primary biliary cirrhosis Differential diagnosis Misdiagnosis Lung cancer Case report

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Multi-junction cascaded vertical-cavity surface-emitting laser with a high power conversion efficiency of 74%

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Light(Science & Applications) 2024年第3期13卷 555-565页

作者： yao Xiao jun Wang Heng Liu Pei Miao Yudan Gou Zhicheng Zhang Guoliang Deng Shouhuan Zhou College of Electronics and Information Engineering Sichuan UniversityChengdu 610064China Suzhou Everbright Photonics Co. LtdSuzhou 215163China

High electro-optical conversion efficiency is one of the most distinctive features of semiconductor lasers as compared to other types of lasers.Its further increase remains a significant objective.Further enhancing the efficiency of edgeemitting lasers(EEL),which represent the highest efficiency among semiconductor lasers at present,is challenging.The efficiency of vertical cavity surface emitting lasers(VCSELs)has always been relatively low compared to EEL.This paper,combining modeling with experiments,demonstrates the potential of multi-junction cascaded VCSELs to achieve high efficiency beyond that of EELs,our simulations show,that a 20-junction VCSEL can achieve an efficiency of more than 88%at room temperature.We fabricated VCSEL devices with different numbers of junctions and compared their energy efficiency.15-junction VCSELs achieved a maximum efficiency of 74%at room temperature under nanosecond driving current,the corresponding differential quantum efficiency exceeds 1100%,being the largest electro-optical conversion efficiency and differential quantum efficiency reported until now for VCSELs.

关键词： cascaded power cavity

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Atomic tailoring-induced deficiency in tungsten oxides for high-performance energy-related devices

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Tungsten 2024年第2期6卷 269-277页

作者： Jing-Huang Lin yao-Tian Yan jun-Lei Qi Chen-Yang Zha Institute of Applied Physics and Materials Engineering Zhuhai UM Science and Technology Research InstituteUniversity of MacaoTaipaMacao SAR 999078China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001China Jiangmen Laboratory of Carbon Science and Technology Hong Kong University of Science and Technology(Guangzhou)Jiangmen 529199China Key Laboratory of Flexible Electronics School of Flexible Electronics(Future Technologies)Institute of Advanced MaterialsNanjing Tech UniversityNanjing 211816China

Nowadays,tungsten oxides,as a typical transition metal oxide,are widely and intensively investigated owing to their excellent material properties and device properties.Controlling oxygen defi ciency in tungsten oxides is typically the key to enhance their performances for a variety of critical technological applications.With a gradual increase of oxygen defi ciency,various non-stoichiometric tungsten oxides can be formed by re-adjustment of the atomic arrangement,which exhibits superior performances than their traditional stoichiometric counterparts.This review mainly focuses on the recent advances in oxygen-defi cient tungsten oxides from the point of atomic structures,including the forming mechanism of non-stoichiometric tungsten oxides and the superiority of these oxygen-defi cient tungsten oxides in energy-related devices.Finally,the challenge and perspective of oxygen-defi cient tungsten oxides are also discussed.

关键词： Oxygen defi ciency Tungsten oxide Atomic structures Catalyst Storage

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Enhancing milk quality and modulating rectal microbiota of dairy goats in starch‑rich diet:the role of bile acid supplementation

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Journal of Animal Science and Biotechnology 2024年第2期15卷 862-877页

作者： Qingyan Yin junjian Yu Jiaxiao Li Tianci Zhang Tianyu Wang Yufei Zhu jun Zhang junhu yao College of Animal Science and Technology Northwest A&F UniversityYangling 712100ShaanxiP.R.China Key Laboratory of Livestock Biology Northwest A&F UniversityYangling 712100ShaanxiP.R.China DAYU Bioengineering(Xi’an)Industrial Development Research Institute Xi’an 710000ShaanxiP.R.China

Background Diets rich in starch have been shown to increase a risk of reducing milk fat content in dairy goats.While bile acids(BAs)have been used as a lipid emulsifier in monogastric and aquatic animals,their effect on ruminants is not well understood.This study aimed to investigate the impact of BAs supplementation on various aspects of dairy goat physiology,including milk composition,rumen fermentation,gut microbiota,and BA metabolism.Results We randomly divided eighteen healthy primiparity lactating dairy goats(days in milk=100±6 d)into two groups and supplemented them with 0 or 4 g/d of BAs undergoing 5 weeks of feeding on a starch-rich diet.The results showed that BAs supplementation positively influenced milk yield and improved the quality of fatty acids in goat milk.BAs supplementation led to a reduction in saturated fatty acids(C16:0)and an increase in monounsaturated fatty acids(cis-9 C18:1),resulting in a healthier milk fatty acid profile.We observed a significant increase in plasma total bile acid concentration while the proportion of rumen short-chain fatty acids was not affected.Furthermore,BAs supplementation induced significant changes in the composition of the gut microbiota,favoring the enrichment of specific bacterial groups and altering the balance of microbial populations.Correlation analysis revealed associations between specific bacterial groups(Bacillus and Christensenellaceae R-7 group)and BA types,suggesting a role for the gut microbiota in BA metabolism.Functional prediction analysis revealed notable changes in pathways associated with lipid metabolism,suggesting that BAs supplementation has the potential to modulate lipid-related processes.Conclusion These findings highlight the potential benefits of BAs supplementation in enhancing milk production,improving milk quality,and influencing metabolic pathways in dairy goats.Further research is warranted to elucidate the underlying mechanisms and explore the broader implications of these findings.

关键词： Bile acids Dairy goats Lipid metabolism Gut microbiota

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Development and application of bionic systems consisting of tumor-cell membranes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第9期23卷 770-777页

作者： Tianjiao PENG jun yao Clinical Medical College Henan University of Science and TechnologyLuoyang471003China Department of Oncology the First Affiliated Hospital of Henan University of Science and TechnologyLuoyang471003China Epigenetics and Molecular Biology Laboratory the First Affiliated Hospital of Henan University of Science and TechnologyLuoyang471003China

Malignant tumors pose a serious threat to human health but during the past decade,great progress has been made in the treatment of tumors.The tumorcell membrane is well constructed and can be used to solve problems in tumor therapy.Tumor-cell membranes exhibit not only high biocompatibility duc to their homology but also enhanced therapeutic ffects when combined with nanotechnology.Meanwhile,nanoma-tcrials show high selectivity,sensitivity,and clinical transformation potential.Enhanced immunotherapy or tumor vaccines have potential clinical application because of tumor-membranc surfacc-specific antigens.Several studies have confirmed the feasibility and ad-vantages of using tumorcell membrane incorporated nanosystems for tumor therapy.Considering all this,we focus in this review on the application of tumor-cell-membrane bionic platforms and,in the summary,provide ideas for new scientific developments.

关键词： membranes tumor clinical

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