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The Role of Propeptide in the Refolding of Human Group IB Phospholipase A2

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 583-588页

作者： Hong-Qiang CHENG1 and Gen-jun xu1,2*Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 2College of Life Science, Zhejiang University of Sciences, Hangzhou 310033, China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences College of Life Science Zhejiang University of Sciences

Human group IB phospholipase A2 (IB-PLA2) and its zymogen (proIB-PLA2) were purified from E. coli. Refolding was carried out by diluting the denatured forms of both IB-PLA2 and proIB-PLA2 with renaturation buffer in which the disulfide bonds were completely reduced. The refolding yield of proIB-PLA2 was increased by about 50% over that of the mature enzyme. The refolding of IB-PLA2 usually produced aggregates under normal conditions, as determined by light scattering. In addition, the unfolding experiments showed that the mature enzyme was more stable than the proenzyme toward denaturants in the presence of DTT. Results suggested that the N-terminal sequence rather than its conformation of human proIB-PLA2 played an important role in the refolding process.

关键词： human group IB phospholipase A2 (IB-PLA2) refolding first disulfide bond propeptide

Impact Experimental Investigation of PVB Laminated Glass Based on High-Speed Photography: An Initial Attempt

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Impact Experimental Investigation of PVB Laminated Glass Bas...

Bayesian inference of neutron-skin thickness and neutron-star observables based on effective nuclear interactions

第八届国际汽车交通安全学术会议

作者： Mengyi ZHU1 Bohan LIU1 Yueting SUN1 jun xu1 Yibing LI1 (1State Key Laboratory of Automotive Safety & Energy Department of Automotive Engineering Tsinghua University Beijing 10084)

In this paper,a high-speed photography method combined with drop-weight test is used to study the dynamic cracking behavior of PVB （polyvinyl butyral） laminated glass subject to low-speed *** patterns,paths and velocities,derived from a series of dynamic photos,are thoroughly analyzed to explore the characteristics cracking *** addition,given the different the experimental conditions,*** crack velocities,cracking speed （including maximum speed and speed-time history curve） are examined and compared.

关键词： PVB laminated windshield high-speed photography crack.

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Science China(Physics,Mechanics & Astronomy) 2024年第8期67卷 97-105页

作者： Jia Zhou jun xu Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China School of Physics Science and Engineering Tongji UniversityShanghai 200092China

We have obtained the constraints on the density dependence of the symmetry energy from neutron-skin thickness data by parityviolating electron scatterings and neutron-star observables using a Bayesian approach,based on the standard Skyrme-HartreeFock(SHF)model and its extension as well as the relativistic mean-field(RMF)*** the neutron-skin thickness data(neutron-star observables)mostly constrain the symmetry energy at subsaturation(suprasaturation)densities,they may more or less constrain the behavior of the symmetry energy at suprasaturation(subsaturation)densities,depending on the energy-density functional *** showing the final posterior density dependence of the symmetry energy,we also compare the slope parameters of the symmetry energy at 0.10 fm-3as well as the values of the symmetry energy at twice saturation density from three effective nuclear *** present work serves as a comparison study based on relativistic and non-relativistic energy-density functionals,for constraining the nuclear symmetry energy from low to high densities using a Bayesian approach.

关键词： symmetry energy neutron skin neutron star

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Phonon-blockade-based multiple-photon bundle emission in a quadratically coupled optomechanical system

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Frontiers of physics 2024年第3期19卷 207-215页

作者： Ye-jun xu Hong Xie Interdisciplinary Research Center of Quantum and Photoelectric Information Chizhou UniversityChizhou 247000China Department of Mathematics and Physics Fujian Jiangxia UniversityFuzhou 350108China

We propose a scheme to realize antibunched multiple-photon bundles based on phonon blockade in a quadratically coupled optomechanical *** adjusting the detunings to match the conditions of phonon blockade in the photon sidebands,we establish super-Rabi oscillation between zero-photon state and multiple-photon states with adjustable super-Rabi frequencies under appropriate single-phonon resonant *** the system dissipation into account,we numerically calculate the standard and generalized second-order functions of the cavity mode as well as the quantum trajectories of the state populations with Monte Carlo simulation to confirm that the emitted photons form antibunched multiple-photon ***,the desirable n-photon states are reconstructed after a direct phonon emission based on phonon blockade,and thus the single-phonon emission heralds the cascade emission of n-photon *** proposal shows that the optomechanical system can simultaneously behave as antibunched multiple-photon emitter and single-phonon *** a nonclassical source could have potential applications in quantum information science.

关键词： multiple-photon bundle emission phonon blockade optomechanical system

A modern survey method for determining live loads based on multi-source and open-access data on the Internet

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Frontiers of Structural and Civil Engineering 2024年第8期18卷 1135-1147页

作者： Chi xu jun CHEN Jie LI School of Civil Engineering Tongji UniversityShanghai 200092China The State Key Laboratory on Disaster Reduction in Civil Engineering Tongji UniversityShanghai 200092China

Sufficient survey data are required to describe the stochastic behaviors of live ***,due to manual and on-site operation required by traditional survey methods,traditional surveys face challenges like occupant resistance,high costs,and long implementation *** study proposes a new survey method to access live load data online and *** samples are acquired from multi-source,open-access and dynamically updated data on the *** change intervals,geometrical dimensions and object quantities are obtained from transaction information,building attributes and virtual reality models on real estate websites,*** object weights are collected from commodity information on e-commerce *** integration of the aforementioned data allows for the extraction of necessary statistics to describe a live load *** proposed method is applied to a live load survey in China,covering 20040 m^(2),with around 90000 samples acquired for object weights and load *** survey results reveal that about 70%−80%of the amplitude statistics are attributable to 1/6 of the total object types.

关键词： live load online survey method data acquisition data integration

An alternative 2D ICEEMDAN-based denoising method and its application in processing magnetic anomaly data

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Geodesy and Geodynamics 2024年第5期15卷 465-476页

作者： jun xu Jinsong Du Changqing Yuan School of Geophysics and Geomatics China University of GeosciencesWuhan 430074China Hubei Subsurface Multi-scale Imaging Key Laboratory China University of GeosciencesWuhan 430074China State Key Laboratory of Geological Processes and Mineral Resources China University of GeosciencesWuhan 430074China Key Laboratory of Geological Survey and Evaluation of Ministry of Education China University of GeosciencesWuhan 430074China

Due to environmental noise and human factors,magnetic data collected in the field often contain various noises and interferences that significantly affect the subsequent data processing and *** Mode Decomposition(EMD),an adaptive multiscale analysis method for nonlinear and non-stationary signals,is widely used in geophysical and geodetic data *** with traditional EMD,Improved Complete Ensemble EMD with Adaptive Noise(ICEEMDAN)is more effective in addressing the problem of mode *** on the principles of 1D ICEEMDAN,this paper presents an alternative algorithm for 2D ICEEMDAN,extending its application to two-dimensional *** effectiveness of the proposed approach is demonstrated through synthetic signal experiments,which show that the 2D ICEEMDAN exhibits a weaker mode mixing effect compared to the traditional bidimensional EMD(BEMD)***,to improve the performance of the denoising method based on 2D ICEEMDAN and preserve useful signals in high-frequency components,an improved soft thresholding technique is *** magnetic anomaly data testing indicates that our denoising method effectively preserves signal continuity and outperforms traditional soft thresholding *** validate the practical application of this improved threshold denoising method based on 2D ICEEMDAN,it is applied to ground magnetic survey data in the Yandun area of *** results demonstrate the effectiveness of the method in removing noise while retaining essential information from practical magnetic anomaly *** particular,practical applications suggest that 2D ICEEMDAN can extract trend signals more accurately than the *** conclusion,as a potential tool for multi-scale decomposition,the 2D ICEEMDAN is versatile in processing and analyzing 2D geophysical and geodetic data.

关键词： 2D ICEEMDAN Mode mixing effect Magnetic anomaly Denoising Improved soft thresholding

Enhanced Flexoelectric Response from Functionally Gradient Electrets Undergoing Crumpling Deformation

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Acta Mechanica Solida Sinica 2024年第4期37卷 541-554页

作者： Jianhua Ma Jinrui xu jun Li Binglei Wang Department of Engineering Mechanics Shandong UniversityJinan250061China

Non-uniform deformation of the dielectric subjected to external forces can induce the flexoelectric effect, a phenomenon that couples electrical polarization to strain gradients. However, limited by the size effects, flexoelectricity is not significant at the macroscale and only becomes catchable at the microscale and nanoscale. In recent work, we obtained a considerable flexoelectric-like response by crumpling the dielectric embedded with charges, i.e., the electret, which significantly improved the flexoelectric effect at the macroscale. In this work, we further optimize the macroscopic performance of the flexoelectric response by applying gradient treatment to the electret films. Specifically, we analytically derive the electromechanical coupling of crumpled electret films with gradients of different thicknesses, charge densities, and Young’s moduli as key design variables. It is shown that the gradient-oriented electret film can be tuned to nearly five times that of a uniform electret film.

关键词： Functionally gradient electret Size effects Crumpling Flexoelectric-like response

青藏高原东北部不同降水梯度下高山林线祁连圆柏径向生长与气候关系的比较

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植物生态学报 2018年第1期42卷 66-77页

作者：宋文琦朱良军张旭王晓春张远东东北林业大学林学院生态研究中心哈尔滨150040 中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态环境重点实验室北京100091

为了了解青藏高原东北部不同降水梯度下,高山林线处的树木径向生长与气候关系是否存在差异,在青海东北部从西北到东南沿降水梯度设置3个高山林线采样点:乌兰县哈里哈图国家森林公园(HL,年降水量217 mm)、都兰县曲什岗(QS,281 mm)和同德... 详细信息

为了了解青藏高原东北部不同降水梯度下,高山林线处的树木径向生长与气候关系是否存在差异,在青海东北部从西北到东南沿降水梯度设置3个高山林线采样点:乌兰县哈里哈图国家森林公园(HL,年降水量217 mm)、都兰县曲什岗(QS,281 mm)和同德县河北林场(HB,470 mm),运用树轮年轮学方法分析林线优势种祁连圆柏(Sabina przewalskii)的径向生长-气候关系随降水梯度的变化规律。结果表明:不同降水梯度下,降水对祁连圆柏径向生长的限制作用差异不明显,但温度对祁连圆柏径向生长的影响存在显著差异。在低降水区域(HL),冬、夏季最低气温主要限制祁连圆柏径向生长,并且在不同气候特征年中无明显变化;在中降水区域(QS),祁连圆柏的径向生长明显受冬季最低气温影响,与低降水区域相比,春、夏季最低气温对祁连圆柏径向生长的限制作用减弱,并且主要限制因子在不同气候特征年存在显著变化;在高降水区域(HB),冬、夏季最低气温对祁连圆柏径向生长的限制作用不显著,而春、秋季最低气温对祁连圆柏径向生长的抑制作用显著增加,并且主要集中在高温年和干旱年。该研究结果并未支持干旱(湿润)区高山林线树木径向生长主要由水分(温度)限制的假说,但是林线处降水量会影响树木生长与温度的关系。随着青藏高原东北部暖湿化加剧,不同地区林线处树木生长的气候限制因子可能存在复杂化趋势。

关键词：高山林线气候限制因子降水梯度祁连圆柏青藏高原树轮指数

Advances in wearable and implantable bioelectronics for precision medicine

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Bio-Design and Manufacturing 2024年第4期7卷 383-387页

作者： Kaichen xu Seung Hwan Ko jun Chen State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310058China Applied Nano and Thermal Science Lab Department of Mechanical EngineeringSeoul National UniversitySeoul 08826Republic of Korea Department of Bioengineering University of CaliforniaLos AngelesLos AngelesCA 90095-1599USA

The continuous pursuit for a better quality of life promotes continuous advancements in intelligent *** wearable and implantable bioelectronics have emerged as an innovative complement to rigid material-based electronic devices[1-3].Due to their distinct advantages in terms of ductile,ultrathin,and biocompatible features,these elastic and soft bioelectronic devices can be seamlessly mounted onto various real or artificial tissues and organs.

关键词： wear mounted pursuit