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Rice Stomatal Closure Requires Guard Cell Plasma Membrane ATP-Binding Cassette Transporter RCN1/OsABCG5

Molecular Plant 2016年第3期9卷 417-427页

作者： Shuichi Matsuda Sho Takano Moeko Sato Kaoru Furukawa Hidetaka Nagasawa Shoko Yoshikawa jun kasuga Yoshihiko Tokuji Kazufumi Yazaki Mikio Nakazono Itsuro Takamure Kiyoaki Kato Department of Agro-environmental Science Obihiro University of Agricultural and Veterinary Medicine 2-11 Nishi Inada Obihiro Hokkaido 080-0834 Japan Department of Food Science Obihiro University of Agricultural and Veterinary Medicine 2-11 Nishi Inada Obihiro Hokkaido 080-0834 Japan Research Institute for Sustainable Humanosphere Kyoto University Gokasho Uji Kyoto 611-0011 Japan Graduate School of Bioagricultural Sciences Nagoya University Furo-cho Chikusa Nagoya Aichi 464-8601 Japan Graduate School of Agriculture Hokkaido University Kita 9 Nishi 9 Kita-ku Sapporo Hokkaido 060-8589 Japan

Water stress is one of the major environmental stresses that affect agricultural production worldwide. Water loss from plants occurs primarily through stomatal pores. Here, we report that an Oryza sativa half-size ATP-binding cassette （ABC） subfamily G protein, RCN 1/OsABCG5, is involved in stomatal closure mediated by phytohormone abscisic acid （ABA） accumulation in guard cells. We found that the GFP-RCN1/ OsABCG5-fusion protein was localized at the plasma membrane in guard cells. The percentage of guard cell pairs containing both ABA and GFP-RCN1/OsABCG5 increased after exogenous ABA treatment, whereas they were co-localized in guard cell pairs regardless of whether exogenous ABA was applied. ABA application resulted in a smaller increase in the percentage of guard cell pairs containing ABA in rcnl mutant （A684P） and RCN1-RNAi than in wild-type plants. Furthermore, polyethylene glycol （drought stress）-inducible ABA accumulation in guard cells did not occur in rcnl mutants. Stomata closure mediated by exogenous ABA application was strongly reduced in rcnl mutants. Finally, rcnl mutant plants had more rapid water loss from detached leaves than the wild-type plants. These results indicate that in response to drought stress, RCN1/OsABCG5 is involved in accumulation of ABA in guard cells, which is indispensable for stomatal closure.

关键词： stomatal closure abscisic acid ABC transporter rice drought stress

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地被菊花Fall Color体细胞胚途径再生、遗传转化及转基因植株的抗寒性检测

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中国农业科学 2006年第7期39卷 1443-1450页

作者：洪波仝征李邱华马超 kasuga Mie YAMAGUCHI-SHINOZA KIKaziko 高俊平中国农业大学观赏园艺与园林系 Biological Resources Division Japan International Research Center for Agricultural Sciences1-1 OhwashiTsukubaIbaraki305-8686 Japan

【目的】培育耐寒性强的地被菊花[Dendranthemagrandiflorum(Ramat.)Kitamura]新材料。【方法】以FallColor品种的幼嫩叶片为外植体,探讨胚状体诱导所需的植物生长调节剂浓度和诱导培养时间等条件。【结果】叶片外植体在添加0.75mg·L-1... 详细信息

【目的】培育耐寒性强的地被菊花[Dendranthemagrandiflorum(Ramat.)Kitamura]新材料。【方法】以FallColor品种的幼嫩叶片为外植体,探讨胚状体诱导所需的植物生长调节剂浓度和诱导培养时间等条件。【结果】叶片外植体在添加0.75mg·L-12,4-D的诱导培养基上诱导15d,再进行分生培养,不仅能够诱导胚性愈伤组织形成,还能够诱导胚状体发生,并进一步诱导芽再生,最终93%的供试外植体通过胚状体途径获得芽的再生。通过根癌农杆菌介导法将35S启动子驱动的逆境诱导转录因子DREB1A基因导入该品种。转化株在低温下的种子发芽率、扦插苗生长以及植株露地越冬生长状况等方面都明显优于对照。【结论】本研究成功地建立了地被菊花FallColor体细胞胚再生途径,并成功地获得了具有越冬耐性的地被菊花转化株系。

关键词：菊花地被菊花体细胞胚遗传转化 AtDREB1A 耐性检测

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根癌农杆菌介导的转录因子DREB1A基因在地被菊花中的遗传转化

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农业生物技术学报 2005年第3期13卷 304-309+408页

作者：洪波,张常青,李邱华,高俊平,kasuga Mie,SHINOZAKI-YAMAGUCHI Kazuko 中国农业大学观赏园艺与园林系中国农业大学观赏园艺与园林系中国农业大学观赏园艺与园林系中国农业大学观赏园艺与园林系 Biological Resources Division Japan International Research Center for Agricultural Sciences 1-1 Ohwashi Tsukuba Ibaraki 305-8686 Japan Biological Resources Division Japan International Research Center for Agricultural Sciences 1-1 Ohwashi Tsukuba Ibaraki 305-8686 Japan 北京100094 北京100094 北京100094 北京100094

以地被菊花(Dendranthema grandiflorum *** Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L6-BA和0.2mg/LNAA组合可获得93.8%的再生芽。将含拟南... 详细信息

以地被菊花(Dendranthema grandiflorum *** Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L6-BA和0.2mg/LNAA组合可获得93.8%的再生芽。将含拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因的植物表达载体pBI-DREB1A导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404,农杆菌介导法遗传转化地被菊花White Snow叶盘,经PCR和PCR-Southern检测,DREB1A基因已整合到转化植株的基因组中。遗传转化过程中,预培养2d后,将OD600=0.5～0.7的根癌农杆菌稀释30倍后侵染叶盘10min,再共培养2d,有利于获得最高遗传转化效率。

关键词：地被菊花再生植株遗传转化

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Influence of waning immunity on vaccination decision-making:A multi-strain epidemic model with an evolutionary approach analyzing cost and efficacy

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Infectious Disease Modelling 2024年第3期9卷 657-672页

作者： Md.Mamun-Ur-Rashid Khan jun Tanimoto Interdisciplinary Graduate School of Engineering Sciences Kyushu UniversityKasuga-koenKasuga-shiFukuoka816-8580Japan Faculty of Engineering Sciences Kyushu UniversityKasuga-koenKasuga-shiFukuoka816-8580Japan Department of Mathematics University of DhakaDhaka1000Bangladesh

In this research,we introduce a comprehensive epidemiological model that accounts for multiple strains of an infectious disease and two distinct vaccination *** stands out as the most effective means to prevent and manage infectious ***,when there are various vaccines available,each with its costs and effectiveness,the decision-making process for individuals becomes ***,the factor of waning immunity following vaccination also plays a significant role in influencing these *** understand how individuals make decisions in the context of multiple strains and waning immunity,we employ a behavioral model,allowing an epidemiological model to be coupled with the dynamics of a decision-making *** base their choice of vaccination on factors such as the total number of infected individuals and the cost-effectiveness of the *** findings indicate that as waning immunity increases,people tend to prioritize vaccines with higher costs and greater ***,when more contagious strains are present,the equilibrium in vaccine adoption is reached more ***,we delve into the social dilemma inherent in our model by quantifying the social efficiency deficit(SED)under various parameter combinations.

关键词： Multistrain epidemic model Vaccination Behavior dynamics Waning immunity Social dilemma

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Assessing mixing uniformity in microreactors via in-line spectroscopy

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Chinese Journal of Chemical Engineering 2024年第2期66卷 119-124页

作者： Shusaku Asano Shinji Kudo Taisuke Maki Yosuke Muranaka Kazuhiro Mae jun-ichiro Hayashi Institute for Materials Chemistry and Engineering Kyushu UniversityKasuga 816-8580Japan Department of Chemical Engineering Graduate School of EngineeringKyoto UniversityJapan

Mixing behavior is critical for enhancing the selectivity of fast chemical reactions in microreactors.A high Reynolds number(Re)improves the mixing rate and selectivity of the reactions,but some exceptions of increasing side product yield with the higher Re have been *** study investigated the mixing uniformity in microreactors with in-line UV-vis spectroscopy to clarify the relationship between reaction selectivity and chaotic mixing with the higher Re.A colorization experiment of thymolphthalein in an acidic solution was conducted with an excess acid amount to the base to indicate a non-uniformly mixed ***-uniformity significantly increased with *** the same time,the degree of mixing,which was measured by a usual decolorization experiment,showed that the mixing rate increased with *** in-line analysis of the Villermaux-Dushman reaction during the mixing clarified that side product yield significantly increased with Re at around 300 and then decreased at around *** results suggest the compensation effect between the mixing uniformity and mixing rate on the selectivity of the mixing-sensitive *** mixing,characterized by a larger Re,can disturb mixing uniformity and,in some cases,decrease reaction selectivity.

关键词： Microreactor Villermaux-Dushman reaction In-line analysis Mixing Mass transfer Uniformity

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Continuous monitoring of char surface activity toward benzene

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Carbon Resources Conversion 2019年第1期2卷 43-50页

作者： Cheolyong Choi Kentaro Shima Shinji Kudo Koyo Norinaga Xiangpeng Gao jun-ichiro Hayashi Interdisciplinary Graduate School of Engineering Sciences Kyushu University6-1Kasuga KoenKasuga 816-8580Japan Institute for Materials and Engineering Chemistry Kyushu University6-1Kasuga KoenKasuga 816-8580Japan Department of Materials Science and Engineering School of EngineeringNagoya UniversityNagoya 464-8601Japan Discipline of Electrical Engineering Energy and PhysicsSchool of Engineering and Information TechnologyMurdoch University90 South StreetMurdochWA 6150Australia Transdisciplinary Research and Education Center of Green Technology Kyushu University6-1Kasuga KoenKasuga 816-8580Japan

Kinetics of thermal decomposition of benzene on lignite-derived char was investigated at 900℃ by applying a new method to continuously monitor the char surface *** vapor was continuously forced to pass through a micro fixed bed of char with residence time as short as 7.6 ms,and then detected continuously by a flame-ionization *** showed the presence of two different types of char surfaces;consumptive Type I surface and non-consumptive(sustainable)Type II *** I surface of a partially CO_(2)-gasified char had an capacity of carbon deposit from benzene over 20 wt%-char and an initial activity(represented by a first-order rate constant)as high as 160 s−*** of them decreased with increasing carbon deposit due to consumption of micropores accessible to benzene,and finally became zero leaving Type II surface that had a very stable activity with rate constant of 4 s−*** chars without gasification had capacities of Type I surfaces smaller by two orders of magnitude than the partially gasified char,while the Type II surfaces had activities similar to that of the partially gasified *** was found that Type II surface converted benzene into not only carbon deposit but also diaromatics and even greater *** of the greater aromatics was unknown because they were deposited onto the reactor wall immediately after passing through the char bed.

关键词： Carbon deposition Aromatics Tar Char Kinetics Pyrolysis Gasification

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Time delay of the appearance of a new strain can affect vaccination behavior and disease dynamics:An evolutionary explanation

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Infectious Disease Modelling 2023年第3期8卷 656-671页

作者： Md.Mamun-Ur-Rashid Khan Md.Rajib Arefin jun Tanimoto Interdisciplinary Graduate School of Engineering Sciences Kyushu UniversityKasuga-koenKasuga-shiFukuoka816-8580Japan Faculty of Engineering Sciences Kyushu UniversityKasuga-koenKasuga-shiFukuoka816-8580Japan Department of Mathematics University of DhakaDhaka1000Bangladesh

The emergence of a novel strain during a pandemic,like the current COVID-19,is a major concern to the healthcare *** most effective strategy to control this type of pandemic is *** previous studies suggest that the existing vaccine may not be fully effective against the new ***,the new strain's late arrival has a significant impact on the disease dynamics and vaccine *** on these issues,this study presents a two-strain epidemic model in which the new strain appears with a time *** considered two vaccination provisions,namely preinfection and post-infection vaccinations,which are governed by human behavioral *** such a framework,individuals have the option to commit vaccination before being infected with the first ***,people who forgo vaccination and become infected with the first train have the chance to be vaccinated(after recovery)in an attempt to avoid infection from the second ***,a second strain can infect vaccinated and unvaccinated *** may have additional opportunities to be vaccinated and to protect themselves from the second strain due to the time *** the cost of the vaccine,the severity of the new strain,and the vaccine's effectiveness,our results indicated that delaying the second strain decreases the peak size of the infected ***,by estimating the social efficiency deficit,we discovered that the social dilemma for receiving immunization decreases with the delay in the arrival of the second strain.

关键词： Vaccination Behavior dynamics Two-strain epidemic Time delay Social efficiency deficit

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Effect of SiO_(2)on loss of catalysis of inherent metallic species in CO_(2)gasification of coke from lignite

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Carbon Resources Conversion 2019年第1期2卷 13-22页

作者： Cheolyong Choi U.P.M.Ashik Shinji Kudo Kazuya Uebo Koyo Norinaga jun-ichiro Hayashi Interdisciplinary Graduate School of Engineering Sciences Kyushu University6-1Kasuga KoenKasuga 816-8580Japan Institute for Materials Chemistry and Engineering Kyushu University6-1Kasuga KoenKasuga 816-8580Japan Process Research Laboratories Nippon Steel&Sumitomo Metal Corporation20-1ShintomiFuttsu 293-8511Japan Department of Materials Science and Engineering School of EngineeringNagoya UniversityNagoyaAichi 464-8601Japan Transdisciplinary Research and Education Center of Green Technology Kyushu University6-1Kasuga KoenKasuga 816-8580Japan

Inherent metallic species retained by coal char or coke,such as Na and Ca,behave as catalysts in *** char/coke normally contains inherent SiO_(2),which can react with the inherent catalysts to form silicates,resulting in catalyst deactivation over the range of pyrolysis,carbonization and gasification,and thereby reducing the char/coke *** present authors simulated the inherent catalyst deactivation experimentally by blending a Victorian lignite with SiO_(2),briquetting the SiO_(2)/lignite blend,carbonizing the briquette,and then gasifying the coke with CO_(2).The kinetic analysis of the gasification employed a comprehensive model,which assumed progress in parallel of non-catalytic and catalytic *** model quantitatively described the measured kinetics of the coke gasification with different SiO_(2)contents over a range of coke conversion up to 99.9%.The kinetic analysis revealed that the SiO_(2)deactivated substantial and entire portions of the most active catalyst and its precursor,respectively,before the gasification(i.e.,during the carbonization).The catalyst deactivation also occurred during the gasification,but mainly following a self-deactivation mechanism that involved no silicates formation.

关键词： Coke Lignite Reactivity CO_(2)gasification Inherent metallic species Catalyst Deactivation SiO_(2)

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Analytical Solutions of Fukui-Ishibashi (FI) Model and Quick-Start (QS) Model

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Journal of Applied Mathematics and Physics 2014年第7期2卷 691-697页

作者： Shinji Kukida jun Tanimoto Naoki Ikegaya Aya Hagishimaors Interdisciplinary Graduate School of Engineering Sciences Kyushu University Kasuga Japan

Through straightforward deduction procedure, we explicitly show analytical solutions for both Fukui-Ishibashi (FI) model and Quick-Start (QS) model, which are fundamental deterministic Cellular Automaton (CA), applied... 详细信息

关键词： Cellular Automaton Analytical Solutions

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Improved Car-Following Model Considering Modified Backward Optimal Velocity and Velocity Difference with Backward-Looking Effect

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Journal of Applied Mathematics and Physics 2021年第2期9卷 242-259页

作者： Md. Anowar Hossain K. M. Ariful Kabir jun Tanimoto Interdisciplinary Graduate School of Engineering Sciences Kyushu University Kasuga-koen Kasuga-shi Fukuoka Japan Department of Mathematics Bangladesh University of Engineering and Technology Dhaka Bangladesh Faculty of Engineering Sciences Kyushu University Kasuga-koen Kasuga-shi Fukuoka Japan

In this paper, a new traffic flow model called the forward-backward velocity difference (FBVD) model based on the full velocity difference model is proposed to investigate the backward-looking effect by applying a modified backward optimal velocity using generalized backward maximum speed. The FBVD model belongs to the family of microscopic models that consider spatiotemporally continuous formulations. Neutral stability conditions of the discrete car-following model are derived using the linear stability theory. The stability analysis results prove that the modified backward optimal velocity has a significant positive effect in stabilizing the traffic flow. Through nonlinear analysis, a kink-antikink solution is derived from the modified Korteweg-de Vries equation of the FBVD model to explain traffic congestion of the model. The validity of this theoretical model is checked using numerical results, according to which traffic jams were found to have been significantly diminished by the introduction of the modified backward optimal velocity.

关键词： Realistic Backward Optimal Velocity Standerized Backward Maximum Speed Honked Effect Backward-Looking Effect Car-Following Modelling

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