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High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S)-2-Octanol Acetate

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Chemical Research in Chinese Universities 2007年第3期23卷 319-324页

作者： ZHANG Gui-rong GAO Ren-jun ZHANG Ai-jun rao Lang CAO Shu-gui Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China

To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of （R, S）-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a screening strategy including two steps, first we selected agar plate with substrate to screen the enzymatic activity; secondly we used a pH indicator to screen the enantioselectivity. This method could rapidly detect favorable mutants with high activity and enantioselectivity. A total of 96. 2% of tedious screening work can be precluded using this screening strategy. It is an effective screening for alkyl ester and can be applied to relative screening researches. The four improved mutants were screened from the mutant esterase library. Their enantioselectivities, activities, and structures were investigated at different temperatures.

关键词： High-throughput screening Enantioselectivity Hyperthermophilic esterase Directed evolution

Alkaline digestion behavior and alumina extraction from sodium aluminosilicate generated in pyrometallurgical process

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International Journal of Minerals,Metallurgy and Materials 2018年第12期25卷 1380-1388页

作者： Bo-na Deng Guang-hui Li jun Luo Jing-hua Zeng Ming-jun rao Zhi-wei Peng Tao Jiang School of Minerals Processing & Bioengineering Central South University

In pyrometallurgical process, Al-and Si-bearing minerals in iron and aluminum ores are easily transformed into sodium aluminosilicates in the presence of Na_2O constituents, which alters the leaching behaviors of Al_2O_3 and SiO_2. It was confirmed that sodium aluminosilicates with different phase compositions synthesized at various roasting conditions were effectively digested in the alkaline digestion process. Under the optimum conditions at temperature of 100–120°C, liquid-to-solid ratio(L/S) of 10:2 mL/g, caustic ratio of 4, and Na_2O concentration of 240 g/L, the actual and relative digestion ratio of Al_2O_3 from the synthesized sodium aluminosilicates reached maximums of about 65% and 95%, respectively, while SiO_2 was barely leached out. To validate the superior digestion property of sodium aluminosilicate generated via an actual process, the Bayer digestion of an Al_2O_3-rich material derived from reductive roasting of bauxite and comprising Na_(1.75) Al_(1.75) Si_(0.25)O_4 was conducted; the relative digestion ratio of Al_2O_3 attained 90% at 200°C.

关键词： alumina sodium aluminosilicate roasting alkaline digestion Bayer process

Development of the fast arcing protection for ECRH system on the HL-2A tokamak

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核聚变与等离子体物理 2007年第1期27卷 44-47页

作者： LI Xiu-juan rao jun WANG Ming-wei WANG Xue-yun LI Bo Southwestern Institute of Physics Chengdu 610225

1 Introduction A series of engineering tests and physical experiments,including ECRH[1],was carried out on the HL-2A tokamak in 2005.A fast arcing protection system[2] was developed to ensure operating of ECRH facilit... 详细信息

关键词：弧阈值响应时间托卡马克装置

Ground-layer Adaptive Optics for the 2.5 m Wide-field and High-resolution Solar Telescope

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Research in Astronomy and Astrophysics 2024年第3期24卷 224-236页

作者： Ying Yang Lan-Qiang Zhang Nan-Fei Yan Jin-Sheng Yang Zhen Li Teng-Fei Song Xue-jun rao Chang-Hui rao Key Laboratory on Adaptive Optics Chinese Academy of SciencesChengdu 610209China Institute of Optics and Electronics Chinese Academy of SciencesChengdu 610209China School of Electronic Electrical and Communication Engineering of the University of Chinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China School of Astronomy and Space Science Nanjing UniversityNanjing 210023China Key Laboratory of Modern A&A Ministry of EducationNanjing 210023China Yunnan Observatories Chinese Academy of SciencesKunming 650216.China

The 2.5 m wide-field and high-resolution solar telescope(WeHoST)is currently under developing for solar *** aims to achieve high-resolution observations over a super-wide field of view(FOV)of5′×5′,and a desired resolution of 0.3″.To meet the scientific requirements of WeHoST,the ground-layer adaptive optics(GLAO)with a specially designed wave front sensing system is as the primary *** introduce the GLAO configuration,particularly the wave front sensing *** analytic method,we simulate the performance of both classical AO and GLAO systems,optimize the wave front sensing system,and evaluate GLAO performance in terms of PSF uniformity and correction improvement across whole *** results indicate that,the classical AO will achieve diffraction-limited resolution;the suggested GLAO configuration will uniformly improve the seeing across the full 5′×5′FOV,reducing the FWHM across the axis FOV to less than0.3″(λ≥705 nm,r0≥11 cm),which is more than two times *** specially designed wave front sensor schedule offers new potential for WeHoST’s GLAO,particularly the multi-FOV GLAO and the flexibility to select the detected *** capabilities will significantly enhance the scientific output of the telescope.

关键词： instrumentation adaptive optics-instrumentation detectors-instrumentation high angular resolution-methods numerical-telescopes-Sun activity

Research on Reactive Power Optimization of Offshore Wind Farms Based on Improved Particle Swarm Optimization

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Energy Engineering 2023年第9期120卷 2013-2027页

作者： Zhonghao Qian Hanyi Ma jun rao jun Hu Lichengzi Yu Caoyi Feng Yunxu Qiu Kemo Ding State Grid Jiangsu Nantong Electric Power Co. Ltd.Nantong226000China College of Energy and Electrical Engineering Hohai UniversityNanjing211100China

The lack of reactive power in offshore wind farms will affect the voltage stability and power transmission quality of wind *** improve the voltage stability and reactive power economy of wind farms,the improved particle swarmoptimization is used to optimize the reactive power planning in wind ***,the power flow of offshore wind farms is modeled,analyzed and *** improve the global search ability and local optimization ability of particle swarm optimization,the improved particle swarm optimization adopts the adaptive inertia weight and asynchronous learning *** the minimum active power loss of the offshore wind farms as the objective function,the installation location of the reactive power compensation device is compared according to the node voltage amplitude and the actual engineering ***,a reactive power optimizationmodel based on Static Var Compensator is established inMATLAB to consider the optimal compensation capacity,network loss,convergence speed and voltage amplitude enhancement effect of *** the compensation methods in several different locations,the compensation scheme with the best reactive power optimization effect is ***,the optimization results of the standard particle swarm optimization and the improved particle swarm optimization are compared to verify the superiority of the proposed improved algorithm.

关键词： Offshore wind farms improved particle swarm optimization reactive power optimization adaptive weight asynchronous learning factor voltage stability

Preparation of blast furnace burdens by composite agglomeration process: effect of distribution of magnetite and hematite concentrates in pelletized and matrix feed

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Journal of Iron and Steel Research International 2020年第12期27卷 1363-1371页

作者： Fo-quan Gu Yuan-bo Zhang Guang-hui Li Qiang Zhong jun Luo Zi-jian Su Ming-jun rao Zhi-wei Peng Tao Jiang School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083HunanChina

Effect of distribution of iron concentrates between pelletized and matrix feed on the preparation of blast furnace burdens from two different kinds of fine iron concentrates (magnetite and hematite) by composite agglomeration process (CAP) was explored. It was found that when the mass ratio of iron concentrate A (magnetite) to iron concentrate B (hematite) in the mixed feed was constant, the proportion of iron concentrate A in the pelletized and matrix feed significantly affected the quality of CAP products. Particularly, as the proportion of iron concentrate A in the pelletized feed increased from 0 to 100%, the yield decreased from 82.11% to 79.19% and the tumbler index decreased from 71.33% to 68.27%. The mineralization characterization results indicated that when 100% iron concentrate A was used as the pelletized feed, the crystallization styles of the outer layer and the inner layer of the pellet were different, and a lot of pores exist around hematite and magnetite phases in the pelletized part, with the weak connection of pelletized and matrix part, resulting in poor strength of agglomeration product.

关键词： Composite agglomeration process Sintering Fine iron concentrate Pelletized feed Blast furnace burden

First generation solar adaptive optics system for 1-m New Vacuum Solar Telescope at Fuxian Solar Observatory

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Research in Astronomy and Astrophysics 2016年第2期16卷 19-26页

作者： Chang-Hui rao Lei Zhu Xue-jun rao Lan-Qiang Zhang Hua Bao Xue-An Ma Nai-Ting Gu Chun-Lin Guan Dong-Hong Chen Cheng Wang jun Lin Zen-Yu Jin Zhong Liu Key Laboratory on Adaptive Optics Chinese Academy of Sciences Laboratory on Adaptive Optics Institute of Optics and Electronics Chinese Academy of Sciences Yunnan Astronomical Observatories Chinese Academy of Sciences

The first generation solar adaptive optics （AO） system, which consists of a fine tracking loop with a tip-tilt mirror （TTM） and a correlation tracker, and a high-order correction loop with a 37-element deformable mirror （DM）, a correlating Shack-Hartmann （SH） wavefront sensor （WFS） based on the ab- solute difference algorithm and a real time controller （RTC）, has been developed and installed at the 1-m New Vacuum Solar Telescope （NVST） that is part of Fuxian Solar Observatory （FSO）. Compared with the 37-element solar AO system developed for the 26-cm Solar Fine Structure Telescope, administered by Yunnan Astronomical Observatories, this AO system has two updates： one is the subaperture arrangement of the WFS changed from square to hexagon; the other is the high speed camera of the WFS and the corre- sponding real time controller. The WFS can be operated at a frame rate of 2100 Hz and the error correction bandwidth can exceed 100 Hz. After AO correction, the averaged residual image motion and the averaged RMS wavefront error are reduced to 0.06＂ and 45 nm, respectively. The results of on-sky testing obser- vations demonstrate better contrast and finer structures of the images taken with AO than those without AO.

关键词： adaptive optics -- high angular resolution -- sunspots -- granulation

Computer Vision-based Navigation and Predefined Track Following Control of a Small Robotic Airship

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自动化学报 2007年第3期33卷 286-291页

作者： XIE Shao-Rong LUO jun rao Jin-jun GONG Zhen-Bang School of Mechatronics Engineering and Automation ShanghaiUniversity Shanghai 200072 P. R. China

为小机器的飞艇，飞艇应该能够跟随一条预定义的轨道，这被要求。在这份报纸，计算机为机器的飞艇的基于视觉的航行和最佳的模糊控制策略被建议。第一，视觉航行基于环境的自然里程碑被介绍。例如，当飞艇在一个城市上正在飞时，大楼能... 详细信息

为小机器的飞艇，飞艇应该能够跟随一条预定义的轨道，这被要求。在这份报纸，计算机为机器的飞艇的基于视觉的航行和最佳的模糊控制策略被建议。第一，视觉航行基于环境的自然里程碑被介绍。例如，当飞艇在一个城市上正在飞时，大楼能被用作其几何性质从数字地图或一个地理信息系统(GIS ) 被知道的视觉烽火。然后，几何方法论被采用关于飞艇的取向和位置提取信息。以便在一个预定义的磁道上保留飞艇，一个模糊飞行控制系统被设计，它把那些数据用作它的输入。并且基因算法(气体) ，一个通用全球优化方法，被利用优化模糊控制器的会员功能。最后，航行和控制策略被验证。

关键词：自动化系统飞行系统智能机器人宇宙飞船

An efficient method for iron ore sintering with high-bed layer:double-layer sintering

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Journal of Iron and Steel Research International 2021年第11期28卷 1366-1374页

作者： Qiang Zhong Hui-bo Liu Liang-ping Xu Xin Zhang Ming-jun rao Zhi-wei Peng Guang-hui Li Tao Jiang School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083HunanChina

Poor permeability and low sintering productivity restrict the development of high-bed *** efficient method of the double-layer sintering process(DLSP)was proposed to achieve high-bed sintering and solve the aforementioned *** calculation and sintering pot experiments were implemented to investigate the double-layer sintering *** sintering process and DLSP were compared in terms of sintering indices,metallurgical properties and morphology *** the condition of traditional sintering process,DLSP successfully realized fast velocity and highly productive sintering of 1000-mm high *** the sintering bed is charged and ignited twice,the air permeability of the bed has been greatly *** time is shortened significantly by simultaneous sintering of the upper and lower feed *** the condition of bed height proportion of 350/650 mm and pre-sintering time of 20 min,the yield,tumbler strength,productivity and solid fuel consumption are 69.96%,65.87%,1.71 t(m^(2)h)^(-1)and 56.71 kg/t,***,hematite,calcium ferrite and complex calcium ferrite are the main phases of DLSP *** metallurgical properties of DSLP products meet the requirement of *** indicates that DLSP is an effective method to solve the disadvantages of bad permeability and low sintering productivity in high-bed sintering.

关键词： Iron ore sintering High-bed sintering Double-layer sintering process Sintering productivity Bed permeability