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Biochemical Estimation of Glucose 6 Phosphate Dehydrogenase Deficiency in Saudi Adults: Different Methods and Its Rationalization

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Advances in Bioscience and Biotechnology 2014年第5期5卷 434-437页

作者： jummanah jarullah Soad AlJaouni Mahesh C. Sharma Bushra M. S. jarullah Department of Biotechnology Kadi Sarva Vishwavidyalaya Ghandinagar India Haematology Research La King Fahd Medical Research Centre King Abdulaziz University Jeddah KSA

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzymopathy affecting 400 million people, globally. G6PD deficiency is an X-linked genetic condition, which is more likely to affect males than females. Heterozygous females go undetected in a commonly used method. The aim of the study was to identify & rationalize different biochemical methods for detections of G6PD deficiency. Methods: Cross section retrospective study was conducted on 1584 (800 males, 784 females) blood samples collected from King Abdulaziz University Hospital (KAUH) and King Fahd Armed force hospital (KFAFH) in Jeddah, Western Saudi Arabia. Blood samples were screened for G6PD activity by fluorescence spot test, semi quantitative color reduction test and spectrometric quantitative evaluation. Hemoglobin (Hb) was measured on the same sample by BC-3200 Auto hematology Analyser. G6PD activity was recorded as U/g Hb. Samples identified as deficient with cutoff ≤4.6 U/gHb. Results: The prevalence of G6PD deficiency identified by fluorescence spot test was 73(4.6%) and all were deficient male. By semi quantitative method, the prevalence rate was 51(3.2%) and again all were male deficit patients. However, when quantitative spectrometric method was used, the prevalence was found in 90(5.7%), where in 73(4.6%) deficient patients were males and 17(1.1%) were females. Conclusion: Since the fluorescence spot test did not miss any G6PD deficient male, it should be restricted to males and quantitative test should be done on females. Each ethnic group should cultivate their own cutoff value for categorization of deficient patients.

关键词： Glucose 6 Phosphate Dehydrogenase Fluorescence Semi-Quantitative & Quantitative

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Prevalence and distribution of <i>Tomato leaf curl virus</i>in major agroclimatic zones of Gujarat

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Advances in Bioscience and Biotechnology 2014年第1期5卷 1-3页

作者： M. Shelat S. Murari M. C. Sharma R. B. Subramanian J. jummanah B. jarullah BRD School of Biosciences Sardar Patel University Vallabh Vidyanagar India Department of Biotechnology Kadi Sarva Vishwavidyalaya Gandhinagar India King Fahd Medical Research Center King Abdulaziz University Jeddah KSA

A study was conducted to establish the prevalence and severity of Tomato leaf curl viral disease in the major agroclimatic tomato cultivating regions of Gujarat. Districts of Gandhinagar, Kheda, Mehsana, Panchmahal and Rajkot of Gujarat were surveyed. The survey aimed at assessing types of symptoms, severity and yield loss in the tomato plants. Results obtained indicate vast spread of Tomato leaf curl virus in majority of the tomato fields in Gujarat. The maximum infection was observed in the Kheda district with almost complete crop loss followed by Mehasna and Gandhinagar districts showing high prevalence of infection. Influence of the climatic conditions and cultural practices were observed on the level of infection.

关键词： Field Survey Geographical Distribution TLCV Yield Loss

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