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Effect of Indole 3-Butyric Acid and Media Type on Adventitious Root Formation in Sheanut Tree (Vitellaria paradoxaC. F. Gaertn.) Stem Cuttings

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American Journal of Plant Sciences 2014年第3期5卷 313-318页

作者： Daniel Brain Akakpo Naalamle Amissah julius yeboah Essie Blay Cocoa Research Institute of Ghana (CRIG) Bole Ghana Department of Crop Science College of Agriculture and Consumer Sciences University of Ghana Accra Ghana

Shea nuts play an important role in food security for rural folks within sub-Sahara Africa, serving as the main source of income for many people living in Northern Ghana. Unfortunately, the full economic potential of the Sheanut tree has not been fully realized due to the difficulty involved in its domestication. This difficulty in vegetatively propagating sheanut trees has greatly hindered its cultivation and the realization of its true economic potential. Two experiments were conducted to investigate the effects of rooting media and varying indole 3-butyric acid (IBA) concentrations on adventitious root formation in cuttings taken from coppiced sheanut trees. Results indicated that 3000 ppm produced significantly (p 0.05) better rooting (57.5%) than 5000 ppm (30%), 7000 ppm (45.0%) and the control (7.5%). Although the levels of soluble sugars (SS) and total free phenols (TFP) in the cutting were significantly (p 0.05) higher at the end of the experiment (after IBA treatment) compared to the start (prior to IBA treatment), the SS and TFP trends observed did not clearly explain the rooting differences found between the IBA levels investigated. Callus formation was significantly (p 0.05) higher (35.0%) in the control (no IBA). Generally, callus formation decreased with increasing IBA concentration. In the rooting media experiment, rooting was significantly (p 0.05) higher in the rice husk medium (35.0%) compared to that in the palm fiber (18.3%), saw dust (14.1%) and top soil (16.7%) media.

关键词： Coppiced Indole 3-Butyric Acid Sheanut Tree Cuttings Rooting Medium

Weaning and Field Survival Responses of Propagules to Propagating Structures and Seedling Types in Sheanut Production in Tropical Africa

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American Journal of Plant Sciences 2014年第15期5卷 2247-2255页

作者： julius yeboah Ben Kwaku Branoh Banful Frank Manu Amoah Bonaventure Kissinger Maalekuu Peter Yaw Boateng Cocoa Research Institute of Ghana (CRIG) Tafo-Akim Ghana Department of Horticulture Kwame Nkrumah University of Science and Technology Kumasi Ghana

The Shea is an economic tree found in West and Central Africa with huge industrial uses in the confectionery, pharmaceutical and cosmetic sectors worldwide. Its rapid multiplication has been hampered by its slow growth and long gestation period. Successes in cutting propagation have been achieved (between 60% - 80%), however weaning of the rooted cuttings for establishment has been a major challenge. Two factorial experiments were carried out in a study in 2012. The objective of this study was to determine the effects of propagating structures and seedling types on the weaning and subsequent field establishment of propagules of Shea tree for plantation establishment. A randomized complete block design with three replications was used. Survival of the rooted cuttings in the mist propagator was very high (93.3%) and comparable to that of the seedlings (100%). The rooted cuttings in mist propagator produced the highest number of leaves, 11 times greater than the least number of leaves produced by seedlings in the lath house. Comparing the seedling types, the rooted cuttings produced significantly greater number of leaves, 4.8 times than the normal seedlings. Rooted cuttings in the mist propagator produced the biggest stem girth significantly greater than the seedlings kept in all the structures. The rooted cuttings in the mist propagator produced 4.4 times bigger stem girths than the seedlings in the lath house which had the smallest stem girth. The rooted cuttings in the mist propagator produced the tallest plants, 1.4 times and 1.9 times significantly taller than the seedlings in the propagating pit and lath house, respectively, which produced the shortest plants. There was a significant relationship between field survival of propagules and the month of establishment expressed as Y (percent survival) = -2844 + 0.070 × (month);P < 0.001;R2 = 0.68;n = 90. Rooted cuttings transplanted in a hole depth of 52.0 cm produced the biggest stem girth, 5.6 times bigger than the

关键词： Vitellaria paradoxa Propagating Structures Seedling Types Hole Depths

Genotypic Effect of Rootstock and Scion on Grafting Success and Growth of Kola (Cola nitida) Seedlings

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American Journal of Plant Sciences 2014年第26期5卷 3873-3879页

作者： Abu Mustapha Dadzie Abraham Akpertey julius yeboah Stephen Yaw Opoku Atta Ofori Samuel Lowor Richard Ackyeampong Patricia Adu- yeboah Mercy Asamoah Frank Manu Amoah Cocoa Research Institute of Ghana (CRIG) Tafo-Akim Ghana

Kola (Cola nitida) is an important economic cash crop for many West and Central African countries. It has several medicinal uses in the pharmaceutical industries and also plays a major role in traditional marriages among Islamic communities across West and Central Africa. The crop is extensively cultivated in Nigeria and Ghana. However, it exhibit signs of total and partial sterility as well as self incompatibility when propagated from seeds in most cases. Therefore, grafting is seen as a method of choice in addressing the problem stated above. Though grafting accounts for some degree of success, there is the need to assess genotypic and physiological factors that account for high or low grafting success. Genetic and physiological factors (such as rootstock age) affecting grafting success and growth in kola (C. nitida) were investigated in two separate experiments. In experiment I720 kola seedlings were raised from unselected kola nuts and sown at two monthly intervals. Four groups of seedlings (180/group) i.e. 6, 8, 10 and 12 months old were thus produced. Three different scions (A1, A12 and JB1) measuring (5-10 cm) were grafted onto the four age groups of rootstocks, namely, 6, 8, 10 and 12 months. Experiment II consisted of 540 seedlings raised from three main crosses (JX1/9 × JX1/11 * B1/142 × B1/151, JX1/9 × JX1/11 * B2/177 × B2/156 and JX1/9 × JX1/11 * GX1/46 × GX1/53). Grafting was done after six months using the same set of scions as described in experiment I. Experimental design used was 3 × 4 and 3 × 3 factorial designs in completely randomised design with three replicates for experiment I and II respectively. The fixed effects were the different genotype and age of rootstock at grafting whilst the response variable was the percentage of successful grafting two months as well as growth at six monthly intervals. Results from the study showed that grafting onto 6 months old stocks gave the highest percentage success and growth of grafts followed by 8, 10 and

关键词： Kola Rootstock Scion Genotype

Rooting Response of Air-Layered Shea (Vitellaria paradoxa) Trees to Media and Hormonal Application under Two Different Climatic Conditions

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American Journal of Plant Sciences 2014年第9期5卷 1212-1219页

作者： julius yeboah Ben Kwaku Branoh Banful Peter Yaw Boateng Frank Manu Amoah Bonaventure Kissinger Maalekuu Samuel Tetteh Lowor Cocoa Research Institute of Ghana Tafo-Akim Ghana Department of Horticulture Kwame Nkrumah University of Science and Technology Kumasi Ghana

Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, hampered by the poor rooting success of its planting material with adverse consequences on field establishment and total fruit yield. A 3 × 2 factorial experiment arranged in a randomized complete block design was carried out in 2012 at Bole in the Interior Savanna agro-ecological zone. The objective was to assess the rooting success of Shea shoots by the air-layering technique using two media types (palm fibre and Sphagnum moss) and three IBA hormone concentrations (0, 5000 and 10,000 ppm) under contrasting climatic (wet and dry) conditions. Layered shoots which were sprayed with 10,000 ppm IBA and wrapped with Sphagnum moss gave significantly (P < 0.05) higher rooting success in terms of more roots per cutting (73.3%) and longer roots per cutting (9.0 cm) than palm fibre at both 5000 ppm (30.0%;3.7 cm) and 10,000 ppm (46.7%;7.9 cm) concentrations. Higher rooting success was significantly achieved in the wet season than in the dry season. Sphagnum moss treated with 10,000 ppm IBA facilitated the translocation of higher levels of sugar and total free phenol (TFP) to the base of the layered shoots which resulted in significantly (P < 0.05) higher number of roots and better roots protection from fungal infection than the other treatments. Rooting of layered shoots was affected to a greater extent by low temperatures (Rooting = 836 – 34.2 Temp (low);R2 = 82%, p < 0.001) than by high temperatures (Rooting = 5250 – 175.0 Temp (high);R2 = 64.5%, p < 0.009). Rooting of layered shoots was also significantly and negatively affected by the spread of the canopy of the selected tree such that closed canopy trees resulted in higher and better rooting that open canopy trees (Rooting = 113.87-23.697(Canopy spread);R2 = 89%, p = 0.002;n = 9). Furthermore, significant and

关键词： Vitellaria paradoxa Rooting IBA Auxin Palm Fibre Sphagnum Peat Moss Air-Layering

Evaluation of Potential Cashew Clones for Utilization in Ghana

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Advances in Biological Chemistry 2014年第4期4卷 232-239页

作者： Abu Mustapha Dadzie Paul Kwesi Krah Adu-Gyamfi Stephen Yaw Opoku julius yeboah Abraham Akpertey Kwabena Opoku-Ameyaw Michael Assuah Esther Gyedu-Akoto Wiseborn Bismark Danquah Cocoa Research Institute of Ghana (CRIG) Tafo-Akim Ghana Department of Plant Pathology University of California Davis USA

Cashew (Anacardium occidentale L) is an important cash crop cultivated by about 3 million households in Africa and serves as the livelihood for many African farmers, especially Ghana. Despite the importance of cashew as a commodity crop with increasing cultivation in Northern Ghana, the crop is challenged with problems such as, low and variable nut yields, low kernel out turn percentage and susceptibility to insect pests as a result of establishing cashew farms with unselected seeds. In order to address the challenges, the Cocoa Research Institute of Ghana established a clonal evaluation trial in Northern Ghana (dry savanna vegetation) to indentify promising clones for subsequent distribution to cashew farmers as an interim measure. The trials consisted of ten different clones planted in Randomised Completed Block Design (RCBD) with four replicates. Parameters evaluated were yield, yield efficiency, nut weight, percentage out turn and canopy area. Data analysis was performed with Gen Stat version 11.0 and the results revealed significant differences (P < 0.05) in the performance of the clones in all the parameters considered. Such differences allowed the identification of promising clones over other clones tested. A few clones combined two or three traits (parameters) which were significantly different from the rest of the clones evaluated. No single clone was found to be significantly different from the rest in terms of all the parameters considered. However a greater proportion of the clones were found to produce yields far above average yields recorded by unselected cashew trees in farmers’ fields. Clones W266 and W278 seem outstanding for most of the parameters considered, though not exclusive.

关键词： Cashew Clone Yield Kernel and Efficiency

Evaluation of Major Chemical Components in Beans of Some Selected International Cocoa Clones in Ghana

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Journal of Agricultural Science and Technology(B) 2014年第3期4卷 200-208页

作者： Abu Mustapha Dadzie Stephen Yaw Opoku Atta Ofori Samuel Lowor Jemmy Felix Takrama Francis Kwame Padi julius yeboah Department of Plant Breeding Cocoa Research Institute of Ghana (CRIG) Tafo-Akim Ghana

Cocoa bean is an important raw material for the chocolate, pharmaceutical and cosmetic industries. The butter content and other chemical components such as polyphenols play essential role in the health benefits of consumers as well as flavor of chocolates produced. In this study cocoa beans from 15 international clones (genotypes) were fermented and dried for six and 14 days, respectively. Genotypic effect on the major chemical components of the beans that affect chocolate flavour and taste were assessed using various protocols in the laboratory. Concentrations and relationship existing among the chemical components tested during three- and two-year periods were determined. Genotypic differences were found to account for variation in the concentration of various components tested. Among the genotypes used Man 15-2 seems to be high in fat content whilst IMC 47 was low. In terms of O-dihydric phenol content ICS 1 recorded the highest whilst MO 20 recorded the least. A further test on polyphenols content showed high levels for Gu 255 whilst T 79/501 recorded the least. Significant positive correlation was also observed among the various chemical components tested. The findings of this work clearly confirmed the existence of significant correlations among the major chemical components in cocoa beans and the influence of genotype on the chemical component concentration. This information could be very useful in breeding and selection for a particular component since genotype plays a major role.

关键词：化学成分可可豆评估克隆国际基因型效应茶多酚含量时间测试