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Modulations of genes related to gut integrity,apoptosis,and immunity underlie the beneficial effects of Bacillus amyloliquefaciens CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model

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Journal of Animal Science and Biotechnology 2021年第2期12卷 626-638页

作者： Kosar Gharib-Naseri juliano cesar de paula dorigam Kiran Doranalli Sarbast Kheravii obert A.Swick Mingan Choct Shu-Biao Wu School of Environmental and Rural Science University of New EnglandArmidaleNSW 2351Australia Evonik Nutrition&Care GmbH 10-B226Rodenbacher Chaussee 463457 Hanau-WolfgangGermany University of New England ArmidaleNSW 2351Australia

Background:The ban of in-feed antimicrobial additives has negatively affected the poultry industry by causing necrotic enteritis(NE)to emerge in the *** such as Bacillus probiotics have shown to be effective on eliminating the negative effects of this *** experiments were conducted to investigate the effect of Bacillus amyloliquefaciens CECT 5940(BA)in broiler chickens under NE challenge and/or fed diets with different protein ***:In both experiments,480 day-old mix-sexed Ross-308 broilers were arranged in a 2×2 factorial arrangement of *** experiment 1,the factors were NE challenge(yes or no)and probiotic(yes or no).In experiment 2,the factors were dietary crude protein levels(standard or reduced)and probiotic(yes or no)and were used under NE challenge *** administration of Eimeria oocysts(day 9)followed by inoculation with Clostridium perfringens(day 14 and 15)was used to induce NE *** day 16,two birds from each treatment were gavaged with fluorescein isothiocyanate-dextran(FITC-d)and blood samples were collected for gut integrity evaluation,and jejunal samples were collected for gene expression ***:In experiment 1,BA supplementation decreased caspase-3(CASP3)(Pde protein ***,BA supplementation downregulated INF-γ(Pde protein ***:These findings suggest that supplementation of BA in broil

关键词： Bacillus amyloliquefaciens Broiler Clostridium perfringens Gene expression Necrotic enteritis Probiotic

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Reply to:“Absorption of methionine sources in animals-is there more to know?”-Yes, there is more to know!

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Animal Nutrition 2023年第3期14卷 67-68页

作者： Andreas Lemme juliano cesar de paula dorigam Stefan Mack Evonik Operations GmbH

Recently,above mentioned paper compiling and discussing literature concerning the absorption of DL-methionine(DL-Met)and two forms of the hydroxy analogue of methionine(2-hydroxy-4-methylthiobutyric acid[HMTBa],Ca-salt of HMTBa[HMTBa-Ca])was published in Animal Nutrition(https://***/10.1016/***.2022.09.004).We would like to comment on this article regarding the aim of the study as expressed in the introduction:“This critical review provides an understanding of diverging studies and provides insights into how further evaluation should be conducted when the bioefficacy of the two molecules is discussed”.

关键词： mentioned concerning sources

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Implications of excreta uric acid concentrations in broilers offered reduced crude protein diets and dietary glycine requirements for uric acid synthesis

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Animal Nutrition 2021年第4期7卷 939-946页

作者： Peter H.Selle David I.Cantor Leon R.McQuade Bernard V.McInerney juliano cesar de paula dorigam Shemil P.Macelline Peter V.Chrystal Sonia Y.Liu Poultry Research Foundation within The University of Sydney CamdenNSW 2570Australia Sydney School of Veterinary Science The University of SydneySydneyNSW 2006Australia Australian Proteome Analysis Facility Macquarie UniversitySydneyNSW 2109Australia Evonik Nutrition and Care GmbH Hanau-Wolfgang 63547Germany School of Life and Environmental Sciences Faculty of ScienceThe University of SydneySydneyNSW 2006Australia Baiada Poultry Pty Limited Pendle HillNSW 2145Australia

In a previous experiment,male Ross 308 broiler chickens were offered dietary treatments with 3 levels of crude protein(222,193,165 g/kg)and 3 feed grains(ground maize,ground wheat,whole wheat)from 7 to 35 d ***-based diets supported superior growth performance in comparison to wheatbased *** acid concentrations in excreta were retrospectively determined and related to total nitrogen(N)excreta *** acid concentrations ranged from 28.5 to 69.4 mg/g and proportions of uric acid-N to total excreta-N ranged from 27.4%to 42.6%in broiler chickens offered the 3×3 factorial array of dietary *** of uric acid-N to total N in excreta in birds offered the165 g/kg CP,maize-based diet were significantly lower by 10.6 percentage units(27.4%versus 38.0%;P=0.00057)than their wheat-based *** excreta analysed had been collected from 35 to37 d post-hatch when feed intakes and excreta outputs were *** were linear relationships between proportions of uric acid-N to total N in excreta in birds offered the three 165 g/kg CP diets with weight gain(r=-0.587;P=0.010),feed intake(r=-0.526;P=0.025)and feed conversion ratios(r=0.635;P=0.005).The possibility that increasing uric acid-N proportions in excreta is indicative of excessive ammonia accumulations compromising growth performance is *** mean proportion of dietary glycine involved in uric acid excretion was 49.2%across all dietary treatments but ranged from 25.0%to 80.9%.Thus,the appropriate amount of dietary glycine is variable and largely dependent on the volume of uric acid synthesised and excreted.

关键词： Ammonia Broiler chicken Glycine Reduced crude protein diet Uric acid

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Assessment of four different dietary amino acid profiles recommended for ISA Brown layer hens: A validation study

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Animal Nutrition 2024年第2期17卷 438-446页

作者： Mehdi Toghyani Shemil Macelline juliano C.de paula dorigam Peter V.Chrystal Peter H.Selle Sonia Y.Liu School of Life and Environmental Sciences Faculty of Science The University of Sydney Poultry Research Foundation The University of Sydney Evonik Operations GmbH

The current feeding study was designed to validate the two dietary essential amino acid profiles(EAAP)established based on linear broken-line(LBL) and quadratic broken-line(QBL) models, in a previous study, against Evonik(AMINOHen) and breeder recommendations for ISA Brown layers for peak production(PP, 20 to 44 weeks of age), and post peak production(post PP, 44 to 75 weeks of age). The EAAP based on LBL models on average had 19.5% and 26.0% lower digestible AA(Lys, Met + Cys, Thr, Trp, Ile and Val), than the EAAP based on QBL models for PP and post PP, respectively. The EAAP based on AMINOHen and breeder recommendation had lower digestible AA than QBL, and higher EAAP than LBL models for both production phases. At 20 weeks of age, 224 ISA Brown layer hens were weighed and randomly allocated to individual battery cages. Each of the four diets was replicated 8 times with 7 birds per replicate. Egg production was recorded daily, and egg weights were measured at the end of each week. Feed consumption was measured at the end of each period. The egg production rate was not significantly affected by the diets and remained at around 98.0%(PP) and 95.0%(post PP)(P > 0.05). Birds fed diets based on LBL recommendation consistently laid smaller eggs, resulting in a lower egg mass(59.8 vs. 62.0 g egg/hen per day during PP, and 60.3 vs. 63.0 g egg/hen per day during post PP; P 0.05). The highest feed conversion ratio(FCR) during PP(P = 0.067) and post PP(P ded for the birds offered diets based on LBL recommendation. In conclusion, all four EAAP tested in this study support an above average egg production rate. However, the EAAP based on LBL models may potentially decrease the input feed cost per kilogram of eggs but are not set to optimise FCR and maximise egg mass.

关键词： Ideal amino acid profile Regression model Egg production Egg mass

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Abiotic stress on seed germination and plant growth of Zeyheria tuberculosa

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林业研究:英文版 2023年第5期34卷 1511-1522页

作者： Robson Luis Silva de Medeiros Rinaldo cesar de paula Joao Vitor Oliveira de Souza Joao Pedro Peixoto Fernandes department of Agricultural Sciences School of Agricultural and Veterinarian SciencesSao Paulo State University(Unesp)14884-900JaboticabalSao PauloBrazil

Zeyheria tuberculosa(Vell.)Bureau ex *** among the Brazilian flora threatened with *** has high-quality wood and can be used to recover herbicidecontaminated ***,abiotic stresses can harm seed germination and seedling development under adverse conditions and need to be understood to improve autochthonous or indigenous biodiversity conservation and strengthen reforestation *** study evaluated the effects of temperature,water and saline stress on *** seed germination and water stress on seedling *** laboratory experiments evaluated germination under:(1)different temperatures(20,25,30,35 and 40℃);(2)different water potentials simulated with polyethylene glycol(PEG6000)at 25 and 30℃;and,(3)different osmotic potentials simulated with NaCl at 25 and 30℃.In addition,a greenhouse experiment subjected seedlings to five water regimes(20%,35%,50%,65%and 80%of maximum substrate water holding capacity).In the laboratory,seedling biometry and germination were evaluated,while physiological and morphological parameters were assessed in the *** potential of seeds increased at 25 and 30℃.Germination and vigor decreased as water and saline stresses *** tolerance thresholds for water and salt stresses were-0.6 and-0.4 MPa,*** the greenhouse,the most restrictive water regimes reduced seedling physiological and morphological *** development was compromised at moisture levels below50%field capacity.

关键词： Bignoniaceae Climate change Ipê-felpudo Ipê-preto Water stress

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Effect of increasing densities of Urochloa brizantha cv. Marandu on Eucalyptus urograndis initial development in silvopastoral system

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Journal of Forestry Research 2019年第2期30卷 537-543页

作者： Michel Anderson Almeida Colmanetti Allan Lopes Bacha Pedro Luis da Costa Aguiar Alves Rinaldo cesar de paula University of Sao Paulo – USP/ESALQ Sao Paulo State University – UNESP/FCAV

In silvopasture system, the coexistence of eucalyptus seedlings with other species may result in growth reduction, especially during eucalyptus early ***, studies elucidating how forage species affect the eucalyptus growth can provide important information for their rational management aiming to obtain the maximum gain of the system. The aim of this work was to evaluate the effect of increasing densities of Urochloa brizantha *** in the early development of Eucalyptus urograndis. An experiment was conducted in 20 L pots, in an open and semi-controlled area, during 90 days after planting of eucalyptus. A completely randomized design with four replications was used, in a 6 9 7 factorial system, meaning six evaluation periods and seven densities of U. brizantha: 0(control), 22, 33, 44, 67, 89 and111 plants m^(-2). Fortnightly, eucalyptus height, stem diameter and chlorophyll fluorescence(Fv/Fm) were evaluated. At the end of experimental period, the net assimilation rate, stomatal conductance and transpiration rate of eucalyptus plants were determined, in addition to the dry matter of eucalyptus(leaves and stem) and U. brizantha (leaves). In coexistence with 111 plants m^(-2), eucalyptus had reduction of 63.9% on total dry matter and 72.7% on leaf area, compared to the control. From the density of22 plants m^(-2), U. brizantha negatively interfere significantly the growth of E. urograndis. Up to 8 plants m^(-2) there are no reductions greater than 5% in eucalyptus height and stem diameter.

关键词： Eucalyptus Palisade grass Competition Interference

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Behavioral study with Erythrina velutina fractions

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Asian Pacific Journal of Tropical Medicine 2009年第5期2卷 30-33页

作者： Silvania Maria Mendes Vasconcelos Natalia Martins Lima Lucas Siqueira de Lucena Alexandre cesar Praxedes Rodrigues Edilberto Rocha Silveira Carlos Clayton Torres Aguiar paula Matias Soares Francisca Clea Florenco de Sousa Glauce Socorro de Barros Viana department of Physiology and Pharmacology Federal University of CearaRua Cel.Nunes de Melo 112760431-270FortalezaCearaBrazil department of Organic Chemistry Federal University of CearaAv.Mister Hull60455-760FortalezaCearaBrazil

Objective:To study the behavioral effects of semi purified fraction prepared from the stem bark of Erythrina velutina on ***:Three chloroformic fractions(CA) at 50 or 100 mg/kg(CA1,CA2 and CA3) were tested in two behavioral tests;plus maze and open ***:In the plus maze test,it was observed a decrease in the number of entries in the closed arms(NECA) CA2 at both doses as compared to the control *** the open field test,CA2 and CA3 fractions decreased the number of squares crossed and the time of permanence on the bar,increased the number of falls at both doses in the rota rod *** CA2 fraction decreased the NECA,but this data did not characterize an anxiolytic *** was the consequence from decrease of locomotor activity(LA) observed in the open field test,that the decrease of LA and rearing,the number of falls and the decrease in time of permanence on the bar in rota rod test were observed after treatment with CA2 and CA3 at both doses,suggesting sedative ***:The present work demonstrated that CA2 and CA3 fractions have impairing effect on LA and motor coordination.

关键词： Erythrina relutina Fraction Locomotor activity Motor coordination

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Physical Accessibility for Disabled People: Analysis of Toilet Facilities in Primary Health Care Units

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Open Journal of Nursing 2016年第11期6卷 948-957页

作者： Aline Cruz Esmeraldo Áfio Luciana Vieira de Carvalho Juliana Freitas Marques paula Marciana Pinheiro de Oliveira Paulo cesar de Almeida Lorita Marlena Freitag Pagliuca Nursing department Federal University of Ceará (UFC) Fortaleza Brazil University of International Integration of the Afro-Brazilian Lusophony (UNILAB) Reden&ccedil&atildeo Brazil State University of Ceará (UECE) Fortaleza Brazil

Background: Accessibility enables the effective participation of disabled persons in public and private areas and the use of urban equipment and street furniture. Objective: This study aimed to analyze the physical accessibility of toilet facilities in urban and rural primary health care units. Methods: It was a quantitative descriptive study conducted in 157 Primary Health Care Units of 16 municipalities in the Baturité Massif region, Ceará, Brazil. The study took place from August 2014 to May 2015, with a checklist type form, designed from the Technical Standard 9050 of the Brazilian National Standards Organization, specifying the ideal access conditions established by law to allow mobility of persons with physical disabilities. Data were processed through the Statistical Package for the Social Sciences software and organized into tables. Results: Toilets designed for physically disabled people were accessible as the location and signs (59.9%), identified with symbols for males and females (57.3%);however, the doorway width was smaller than needed to accommodate a wheelchair (77.7%). Inside the bathroom, only the forward approach was possible (59.9%). Grab bars positioned on the side and rear walls were inadequate or nonexistent (67.6%);toilet seats (91.1%) and toilet paper dispensers (96.2%) were mostly in inaccessible heights;flush controls in appropriate height (59.2%) and activated by light pressure (58%). Sinks without pedestal (51%), but higher than recommended (80.3%) and without single handle faucets (95.6%). It was verified that the toilets of basic health units located in urban areas had better accessibility conditions compared to those in rural areas. Conclusion: Results showed that the analyzed units presented physical inaccessibility in some toilet facilities, making it difficult or even impossible the accessibility for the disabled. The inclusion of accessibility features in health services for this clientele provides equal opportunities and social i

关键词： Health Services Accessibility Disabled Persons Toilet Facilities

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Socio-Economic Profile of People with Disabilities: A Health Impact

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Health 2015年第5期7卷 633-638页

作者： paula Marciana Pinheiro de Oliveira Monaliza Ribeiro Mariano Lorita Marlena Freitag Pagliuca Jacqueline Mota da Silva Paulo cesar de Almeida Giselly Oseni Barbosa Oliveira department of Nursing Universidade da Integracao Internacional da Lusofonia Afro-Brasileira Fortaleza Brazil department of Nursing Universidade Federal do Ceará Fortaleza Brazil department of Nursing Universidade Estadual do Ceará Fortaleza Brazil

The objective was to compare the socioeconomic profile and the repercussions for effects on the health of people with physical, hearing and visual of the main Ceará state associations in Brazil. Quantitative study carried out from March to August 2012 through a structured questionnaire with 120 subjects. Variables were: gender, age, education, family income and marital status. Studies are necessary to examine the effects of sociodemographic characteristics on health. The results indicate that the three deficiencies have different distributions in all age groups (p = 0.001), and auditory and visual obtained the highest percentage in the age group of 18 - 29 years (67.5% and 40%, respectively), while motor in the range of 30 - 49 (60%), if no association between level of education and disability was found (p = 0.553). Family income showed a statistically significant difference between disability (p = 0.044), with lower income in hearing loss (45%) and higher in the visual (42.5%). Studies have demonstrated that people with disabilities are not homogeneous. It is important to examine socioeconomic indicators, and individual needs and characteristics to plan effective action.

关键词： Disabled Persons Health Promotion Nursing

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Weed Interference in the Morphophysiological and Nutritional Characteristics of Erva-Mate(Ilex paraguariensis)

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American Journal of Plant Sciences 2018年第3期9卷 435-445页

作者： Felipe Adelio de David Leandro Galon Gismael Francisco Perin Germani Concenco Alfredo Castamann cesar Tiago Forte paula Rochelly de David Altemir Jose Mossi Andre Andres Ivana Santos Moisinho Federal University of the Southern Border Campus ErechimErechim/RS/BRBrazil Pelotas-RSBrazil

The erva-mate (Ilex paraguariensis) represents an alternative crop for agricultural exploitation in South Brazilian farms with potential to export the harvested product. However, there is scarce information about the interference caused by weeds on this crop. The objective of this work was to evaluate the interference of weed species on the morphophysiological and nutritional characteristics of erva-mate. The experiment was installed in greenhouse at the Federal University of Fronteira Sul (UFFS), Campus Erechim/RS, Brazil, in randomized blocks design, arranged in a 4 × 6 factorial scheme, with four replications. In factor A, weed species (Urochloa plantaginea—Alexandergrass, Bidens pilosa—Hairy beggarticks, Ipomoea indivisa—Morning glory and Conyza bonariensis—Hairy fleabane) were allocated, and in B the populations of these species competing with the erva-mate (0, 1, 2, 3, 4 and 5 plants per pot) were considered. The variables evaluated in erva-mate were sub-stomatal CO2 concentration, photosynthetic rate, CO2 consumed, stomatal conductance, transpiration rate, water use efficiency, height, stem diameter, leaf area and shoot dry mass. Concentrations of nitrogen (N), phosphorus (P), calcium (Ca) and magnesium (Mg) were also evaluated. Overall, all weed species and densities among the tested ones harmed the development of erva-mate plants, which was considered as low competitive with weeds.

关键词： Urochloa plantaginea Bidens pilosa Ipomoea indivisa Conyza bonariensis

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