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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal science and Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella Karina canon‑Beltran Yulia N.cajas Meriem Hamdi Encina M.Gonzalez juliano c.da s.lveira claudia L.V.Leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of Biochemistry and Molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of Veterinary Medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

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Hydrocarbon indication in Rio Bonito Formation sandstone:Implication for cO_(2)storage in são Paulo,Brazil

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Energy Geoscience 2024年第1期5卷 331-341页

作者： Richardson M.Abraham-A Haline V.Rocha saulo B.de Oliveira colombo c.G.Tassinarri Orlando c.da silva Institute of Energy and Environment University of São PauloSão PauloBrazil Research centre for Greenhouse Gas Innovation University of São PauloSão PauloBrazil

são Paulo state has witnessed cO_(2)storage-based investigations considering the availability of suitable geologic structures and proximity to primary cO_(2)source sinks related to bioenergy and carbon capture and storage(BEccs)*** current study presents the hydrocarbon viability evaluations and cO_(2)storage prospects,focusing on the sandstone units of the Rio Bonito *** objective is to apply petrophysical evaluations with geochemical inputs in predicting future hydrocarbon(gas)production to boost cO_(2)storage within the study *** study used data from eleven wells with associated wireline logs(gamma ray,resistivity,density,neutron,and sonic)to predict potential hydrocarbon accumulation and fluid mobility in the investigated *** samples(shale and carbonate)obtained from depths>200 m within the study location have shown bitumen *** geochemistry data of the Rio Bonito Formation shale beds suggest they are potential hydrocarbon source rocks and could have contributed to the gas accumulations within the sandstone *** drilled well data,e.g.,cB-1-sP and TI-1-sP,show hydrocarbon(gas)presence based on the typical resistivity and the combined neutron-density responses at depths up to 3400 m,indicating the possibility of other hydrocarbon members apart from the heavy oil(bitumen)observed from the near-surface rocks *** the three-dimensional(3-D)model,the free fluid indicator(FFI)is more significant towards the southwest and southeast of the area with deeper depths of occurrence,indicating portions with reasonable hydrocarbon recovery rates and good prospects for cO_(2)injection,circulation and permanent ***,future studies based on contemporary datasets are required to establish the hydrocarbon viability further,foster gas production events,and enhance cO_(2)storage possibilities within the region.

关键词： ParanáBasin Hydrocarbon indication sandstone reservoirs Rio Bonito Formation cO_(2)storage Hydrocarbon recovery factor Fluid injection rate

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Resource management at the network edge for federated learning

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Digital communications and Networks 2024年第3期10卷 765-782页

作者： silvana Trindade Luiz F.Bittencourt Nelson L.s.da Fonseca Institute of computing State University of CampinasCampinasBrazil

Federated learning has been explored as a promising solution for training machine learning models at the network edge,without sharing private user *** limited resources at the edge,new solutions must be developed to leverage the software and hardware resources as the existing solutions did not focus on resource management for network edge,specially for federated *** this paper,we describe the recent work on resource manage-ment at the edge and explore the challenges and future directions to allow the execution of federated learning at the *** such as the discovery of resources,deployment,load balancing,migration,and energy effi-ciency are discussed in the paper.

关键词： Resource management Edge computing Federated learning Machine learning

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Analysis of a passive ankle exoskeleton for reduction of metabolic costs during walking

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Defence Technology（防务技术） 2024年第7期37卷 62-68页

作者： Luís Quinto Pedro Pinheiro sergio B.Goncalves Ivo Roupa Paula simoes Miguel Tavares da silva cINAMIL Academia MilitarInstituto Universitario MilitarLisbonPortugal IDMEc Instituto Superior TecnicoUniversidade de LisboaLisbonPortugal

Modern conflicts demand substantial physical and psychological exertion,often resulting in fatigue and diminished combat or operational *** exoskeletons have been developed recently to address these challenges,presenting various limitations that affect their operational or everyday *** article evaluates the performance of a dual-purpose passive ankle exoskeleton developed for the reduction of metabolic costs during walking,seeking to identify a force element that could be applied to the target *** on the 6-min walk test,twenty-nine subjects participated in the study using three different force *** results indicate that it is possible to reduce metabolic expenditure while using the developed ***,the comfort and range of motion results verify the exoskeleton's suitability for use in uneven terrain and during extended ***,the choice of the force element should be tailored to each user,and the control system should be adjustable to optimise the exoskeleton's performance.

关键词： Ankle passive exoskeleton Metabolic cost reduction Dual-use exoskeleton Gait Biomechanics

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study on 6-DOF active vibration-isolation system of the ultra-precision turning lathe based on GA-BP-PID control for dynamic loads

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Advances in Manufacturing 2024年第1期12卷 33-60页

作者： Bo Wang Zhong Jiang Pei-da Hu Department of Precision Instrument Tsinghua UniversityBeijing100084People’s Republic of China Institute of Machinery Manufacturing Technology China Academy of Engineering PhysicsMianyang621900SichuanPeople’s Republic of China

The vibration disturbance from an external environment affects the machining accuracy of ultra-precision machining *** active vibration-isolation systems(AVIs)have been developed based on static *** a vibration-isolation load changes dynamically during ultra-precision turning lathe machining,the system parameters change,and the efficiency of the active vibration-isolation system based on the traditional control strategy *** solve this problem,this paper proposes a vibration-isolation control strategy based on a genetic algorithm-back propagation neural network-PID control(GA-BP-PID),which can automatically adjust the control parameters according to the machining ***-isolation simulations and experiments based on passive vibration isolation,a PID algorithm,and the GA-BP-PID algorithm under dynamic load machining conditions were *** experimental results demonstrated that the active vibration-isolation control strategy designed in this study could effectively attenuate vibration disturbances in the external environment under dynamic load *** design is reasonable and feasible.

关键词： Ultra-precision diamond turning lathe Active vibration isolation six degrees of freedom Dynamic load Genetic algorithm-back propagation neural network-PID(GA-BP-PID)control

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Geochemistry and depositional environment of fuchsite quartzites from sargur Group,western Dharwar craton,India

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Acta Geochimica 2024年第3期43卷 555-570页

作者： c.s.sindhuja G.Harshitha c.Manikyamba K.s.V.subramanyam csIR(council of scientific and Industrial Research)-National Geophysical Research Institute(NGRI) Uppal RoadHyderabad 500007India

Meso-Neoarchean fuchsite quartzites are present in different stratigraphic positions of Dharwar craton including the oldest(~3.3 Ga)sargur Group of western Dharwar *** present study deals with the petro-graphic and geochemical characteristics of the fuchsite quartzites from the Ghattihosahalli belt to evaluate their genesis,depositional setting and the enigma involved in the ancient sedimentation *** major mineral assemblages include quartz,fuchsite,and feldspars along with accessory kyanite and *** geochemical com-positions are characterized by high siO_(2),Al_(2)O_(3),low MgO,caO,strongly enriched cr(1326–6899 ppm),Ba(1165–3653 ppm),sr(46–210 ppm),V(107–868 ppm)and Zn(11–158 ppm)contents compared to the upper continental crust(Ucc).The Ucc normalized rare earth element(REE)patterns are characterized by depleted light REE[(La/sm)Ucc=0.33–0.95]compared to heavy REE[(Gd/Yb)_(Ucc)=0.42–1.65]with conspicuous positive Eu-anomalies(Eu/Eu^(*)=1.35–18.27)characteristic of hydrothermal solutions evidenced through the interlayered *** overall major and trace element systematics reflect a combined mafic-felsic provenance and suggest their deposition at a passive continental margin *** comprehensivefield,petrographic,and geo-chemical studies indicate that these quartzites are infiltrated by cr-richfluids released during high-grade metamorphism of associated ultramafic *** sargur and the subse-quent Dharwar orogeny amalgamated diverse lithounits from different tectonic settings,possibly leading to the release of cr-richfluids and the formation of fuchsite quartzite during or after the *** sug-gest a pre-existing stable crust prior to the sargur Group and the link between orogenic events and various mineral deposits in the Dharwar craton.

关键词： Dharwar craton Ghattihosahalli Fuchsite quartzite Provenance Depositional setting

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Dragonfly Interaction Algorithm for Optimization of Queuing Delay in Industrial Wireless Networks

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Journal of Bionic Engineering 2024年第1期21卷 447-485页

作者： sanjay Bhardwaj da-Hye Kim Dong-seong Kim Department of IT convergence Engineering ICT Convergence Research CenterKumoh National Institute of TechnologyGumiSouth Korea

In industrial wireless networks,data transmitted from source to destination are highly *** often leads to the queuing of the data,and poor management of the queued data results in excessive delays,increased energy consumption,and packet ***,a nature-inspired-based Dragonfly Interaction Optimization Algorithm(DMOA)is proposed for optimization of the queue delay in industrial wireless *** term“interaction”herein used is the characterization of the“flying movement”of the dragonfly towards damselflies(female dragonflies)for *** a result,interaction is represented as the flow of transmitted data packets,or traffic,from the source to the base *** includes each and every feature of dragonfly movement as well as awareness of the rival dragonflies,predators,and damselflies for the desired optimization of the queue *** features are juxtaposed as noise and interference,which are further used in the calculation of industrial wireless metrics:latency,error rate(reliability),throughput,energy efficiency,and fairness for the optimization of the queue *** analysis,convergence analysis,the Wilcoxon test,the Friedman test,and the classical as well as the 2014 IEEE congress of Evolutionary computation(cEc)on the benchmark functions are also used for the evaluation of DMOA in terms of its robustness and *** results demonstrate the robustness of the proposed algorithm for both classical and benchmarking functions of the IEEE cEc ***,the accuracy and efficacy of DMOA were demonstrated by means of the convergence rate,Wilcoxon testing,and ***,fairness using Jain’s index in queue delay optimization in terms of throughput and latency,along with computational complexity,is also evaluated and compared with other *** results show that DMOA exceeds other bio-inspired optimization algorithms in terms of fairness in queue delay management and average packet *** proposed a

关键词： Dragonfly damselfly Interaction Queuing delay Optimization Industrial wireless networks

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Early Phases of star Formation: Testing chemical Tools

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Research in Astronomy and Astrophysics 2024年第1期24卷 96-118页

作者： N.c.Martinez s.Paron cONIcET-Universidad de Buenos Aires.Instituto de Astronomía y Física del Espacio Ciudad Universitaria(C1428EGA)Ciudad Autónoma de Buenos AiresArgentina

star-forming processes strongly influence the IsM ***,many high-quality databases are available at millimeter *** them,it is possible to carry out studies that review and deepen previous *** these studies involve large samples of sources,it is preferred to use direct tools to study the molecular *** the aim of testing these tools such as the use of the HcN/HNc ratio as a thermometer,and the use of H^(13)cO^(+),Hc_(3)N,N_(2)H^(+) and c_(2)H as "chemical clocks," we present a molecular line study toward 55 sources representing massive young stellar objects at different evolutionary stages:infrared dark clouds(IRDcs),highmass protostellar objects(HMPOs),hot molecular cores(HMcs) and ultracompact H II *** found that the use of the HcN/HNc ratio as a universal thermometer in the IsM should be taken with care because the HcN optical depth is a big issue that can affect the ***,this tool should be utilized only after a careful analysis of the HcN spectrum,checking that no line,neither the main nor the hyperfine ones,presents absorption *** point out that the analysis of the emission of H^(13)cO^(+),Hc_(3)N,N_(2)H^(+) and c_(2)H could be useful to trace and distinguish regions among IRDcs,HMPOs and *** molecular line widths of these four species increase from the IRDc to the HMc stage,which can be a consequence of the gas dynamics related to the starforming processes taking place in the molecular *** results not only contribute with more statistics,acting as a probe of such chemical tools,useful to obtain information in large samples of sources,but also complement previous works through the analysis of other types of sources.

关键词： stars:formation IsM:molecules IsM:clouds

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seasonal Variation of the sea surface Temperature Growth Rate of ENsO

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Advances in Atmospheric sciences 2024年第3期41卷 465-477页

作者： Xinyi XING Xianghui FANG da PANG chaopeng JI Department of Atmospheric and Oceanic sciences and Institute of Atmospheric sciences Fudan UniversityShanghai 200438China shanghai Frontiers science center of Atmosphere-Ocean Interaction Shanghai 200438China shanghai Key Laboratory of Ocean-land-atmosphere Boundary Dynamics and climate change Fudan UniversityShanghai 200438China

El Ni?o–southern Oscillation(ENsO) exhibits a distinctive phase-locking characteristic, first expressed during its onset in boreal spring, developing during summer and autumn, reaching its peak towards winter, and decaying over the next spring. several studies have demonstrated that this feature arises as a result of seasonal variation in the growth rate of ENsO as expressed by the sea surface temperature(ssT). The bias towards simulating the phase locking of ENsO by many state-of-the-art climate models is also attributed to the unrealistic depiction of the growth rate. In this study, the seasonal variation of ssT growth rate in the Ni?o-3.4 region(5°s–5°N, 120°–170°W) is estimated in detail based on the mixed layer heat budget equation and recharge oscillator model during 1981–2020. It is suggested that the consideration of a variable mixed layer depth is essential to its diagnostic process. The estimated growth rate has a remarkable seasonal cycle with minimum rates occurring in spring and maximum rates evident in autumn. More specifically, the growth rate derived from the meridional advection(surface heat flux) is positive(negative) throughout the year. Vertical diffusion generally makes a negative contribution to the evolution of growth rate and the magnitude of vertical entrainment represents the smallest contributor. Analysis indicates that the zonal advective feedback is regulated by the meridional immigration of the intertropical convergence zone, which approaches its southernmost extent in February and progresses to its northernmost location in september, and dominates the seasonal variation of the ssT growth rate.

关键词： ssT growth rate intertropical convergence zone zonal advective feedback mixed layer depth ENsO seasonal variation

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Leaf habit differentiation explains trait tradeoffs across savanna woody plants

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Forest Ecosystems 2024年第3期11卷 287-295页

作者： Yangsiding Wang da Yang German Vargas G. Guangyou Hao Jennifer s.Powers Yan Ke Qin Wang Yunbing Zhang Jiaolin Zhang cAs Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnan 666303China University of chinese Academy of sciences Beijing 100049China school of Biological sciences University of UtahSalt Lake CityUT 84112USA cAs Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China Department of Ecology Evolution and BehaviorUniversity of MinnesotaSt PaulMN 55108USA

Identifying how leaf habit subdivisions link to the fast–slow and avoidance–tolerance trait tradeoffs can provide new insight into divergence in ecophysiological strategies among plant functional groups. Here, we tested a hypothesis that the differentiation across deciduous, semi-deciduous and evergreen woody species contributes to physiological trait tradeoffs in a dry-hot valley savanna. We investigated 11 photosynthetic, morphological and hydraulic traits of 24 species including 8 deciduous, 10 semi-deciduous and 6 evergreen species. Deciduous species were grouped in the fast and avoidance side associated with high values of maximum photosynthetic rates,stomatal conductance and leaf size, while evergreen species were grouped in the slow and tolerance side associated with high photosynthetic water use efficiency, leaf mass per area, sapwood density, Huber value, leaf water potential at turgor loss point and water potential causing 50% loss of stem hydraulic conductance. semideciduous species generally had intermediate trait values and represented different physiological characteristics when compared to deciduous and evergreen species. The physiological trait tradeoffs showed a close linkage to the differentiation of these three leaf habits. Our findings clearly reveal trait tradeoffs related to fast–slow and avoidance–tolerance strategies among diverse savanna plants, suggesting a syndrome in multiple ecophysiology strategies across different leaf habits.

关键词： Dry-hot habitat Gas exchange Hydraulics Phenology Tolerance Valley savanna

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