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Phylogeny,identification and pathogenicity of the Botryosphaeriaceae associated with collar and root rot of the biofuel plant Jatropha curcas in Brazil,with a description of new species of Lasiodiplodia

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Fungal Diversity 2014年第4期67卷 231-247页

作者： Alexandre reis machado Danilo Batista Pinho Olinto Liparini Pereira Departamento de Fitopatologia Universidade Federal de VicosaVicosa 36570-000Brazil

The global expansion of cultivation areas of Jatropha has contributed to the emergence of various *** in Brazil,the occurrence of a new disease has been reported that not only reduces the productivity but also causes the death of *** disease is associated with collar and root rot of *** morphological and phylogenetic studies(based on Internal Transcribed Spacers,β-tubulin and Translation Elongation Factor 1-αsequences),nine species of Botryosphaeriaceae were *** species include Lasiodiplodia egyptiacae,***,***,Macrophomina phaseolina,Neoscytalidium hyalinum and four Lasiodiplodia *** are proposed as new species(***,***,*** and ***).All the species in this study,except ***,are *** results show that root rot of physic nut plants is caused by complex *** study provides new information for future studies of disease management,quarantine programs and,especially,the development of resistant varieties for collar and root rot disease in ***.

关键词： Aetiology Botryosphaeriales Dothideomycetes Phylogeny Taxonomy Tropical fungi

How do greenhouse gas emissions vary with biofertilizer type and soil temperature and moisture in a tropical grassland?

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Pedosphere 2020年第5期30卷 607-617页

作者： Abmael da Silva CARDOSO juliana Bega JUNQUEIRA Ricardo Andrade reis Ana Claudia RUGGIERIAnimal Science Departament College of Agricultural and Veterinarian SciencesSao Paulo State UniversityJaboticabal 14884-900(Brazil) Kenji Agricultural and Environmental Consultancy Ribeirdo Preto 14010-079(Brazil)

Greenhouse gases are known to play an important role in global *** this study,we determined the effects of selected soil and climate variables on nitrous oxide(N2O),methane(CH4),and carbon dioxide(CO2)emissions from a tropical grassland fertilized with chicken slurry,swine slurry,cattle slurry,and cattle *** N2O emissions did not differ between treatments and varied from 29.26 to 32.85 mg N m^-***,cumulative CH4 emissions were not significantly different among the treatments and ranged from 6.34 to 57.73 mg CH4 m^-*** and compost application induced CO2 emissions that were significantly different from those in the control *** CH4 conversion factors measured were 0.21%,1.39%,4.39%,and 5.07%for cattle compost,chicken slurry,swine slurry,and cattle slurry,respectively,differing from the recommendations of the Intergovernmental Panel on Climate Change(IPCC).The fraction of added Nemitted as N2O was 0.39%,which was lower than the IPCC default value of 2%.Our findings suggest that N2O emissions could be mitigated by replacing synthetic fertilizer sources with either biofertilizer or *** results indicate the following:N2O emission was mainly controlled by soil temperature,followed by soil moisture and then soil NH^"content;CH4 fluxes were mainly controlled by soil moisture and chamber headspace temperature;and CO2 fluxes were mainly controlled by chamber headspace temperature and soil moisture.

关键词： emission factor methane conversion factor methane oxidation methane production capacity slurry fertilizer soil moisture

Long noncoding RNAs:a missing link in osteoporosis

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Bone Research 2019年第1期7卷 25-40页

作者： Andreia machado Silva Sara reis Moura JoseHenrique Teixeira Mario Adolfo Barbosa Susana Gomes Santos Maria Ines Almeida i3S-Instituto de Investigacao e Inovacao em Sau.de University of PortoPortoPortugal INEB-Instituto de Engenharia Biomedica University of PortoPortoPortugal ICBAS-Instituto de Ciências Biomedicas Abel Salazar University of PortoPortoPortugal

Osteoporosis is a systemic disease that results in loss of bone density and increased fracture risk,particularly in the vertebrae and the *** condition and associated morbidity and mortality increase with population *** noncoding(lnc)RNAs are transcripts longer than 200 nucleotides that are not translated into proteins,but play important regulatory roles in transcriptional and post-transcriptional *** contribution to disease onset and development is increasingly ***,we present an integrative revision on the studies that implicate lncRNAs in osteoporosis and that support their potential use as therapeutic ***,current evidence on lncRNAs involvement in cellular and molecular mechanisms linked to osteoporosis and its major complication,fragility fractures,is *** analyze evidence of their roles in osteogenesis,osteoclastogenesis,and bone fracture healing events from human and animal model ***,the potential of lncRNAs alterations at genetic and transcriptomic level are discussed as osteoporosis risk factors and as new circulating biomarkers for ***,we conclude debating the possibilities,persisting difficulties,and future prospects of using lncRNAs in the treatment of osteoporosis.

关键词： Osteoporosis density lncRNAs

Burnett Order Stress and Spatially-Dependent Boundary Conditions for the Lattice Boltzmann Method

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Communications in Computational Physics 2020年第1期27卷 167-197页

作者： Timothy reis School of Computing and Mathematical Sciences University of GreenwichLondon SE109LSUK

Stress boundary conditions for the lattice Boltzmann equation that are consistent to Burnett order are proposed and imposed using a moment-based *** accuracy of the method with complicated spatially-dependent boundary conditions for stress and velocity is investigated using the regularized lid-driven cavity *** complete set of boundary conditions,which involve gradients evaluated at the boundaries,are implemented locally.A recently-derived collision operator with modified equilibria and velocity-dependent collision rates to reduce the defect in Galilean invariance is also *** results are in excellent agreement with existing benchmark data and exhibit second-order *** lattice Boltzmann stress field is studied and shown to depart significantly from the Newtonian viscous stress when the ratio of Mach to Reynolds numbers is not negligibly small.

关键词： Lattice Boltzmann method moment based boundary conditions Burnett stress reg-ularized cavity

Furazolidone-based triple therapy for H pylori gastritis in children

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World Journal of Gastroenterology 2006年第34期12卷 5544-5549页

作者： Elisabete Kawakami Rodrigo Strehl machado Silvio Kazuo Ogata Marini Langner Erika Fukushima Anna Paula Carelli Vania Cláudia Guimares Bonucci Francy reis Silva Patrício Peptic Diseases Outpatient Clinic Pediatric Gastroenterology Division Department of Pediatric Universidade Federal de So Paulo/Escola Paulista de Medicina So Paulo SP Brazil Department of Pathology Universidade Federal de So Paulo/Escola Paulista de Medicina S?o Paulo SP Brazil

AIM： To evaluate the furazolidone-based triple therapy in children with symptomatic H pylori gastdtis. METHODS： A prospective and consecutive open trial was carried out. The study included 38 patients with upper digestive symptoms sufficiently severe to warrant endoscopic investigation. H pylori status was defined based both on histology and on positive ^13C-urea breath test. Drug regimen was a seven-day course of omeprazole, clarithromycin and furazolidone （100 mg, 200 mg if over 30 kg） twice daily. Eradication of H pylori was assessed two months after treatment by histology and ^13C-urea breath test. Further clinical evaluation was performed 7 d, 2 and 6 mo after the treatment. RESULTS： Thirty-eight patients （24 females, 14 males） were included. Their age ranged from 4 to 17.8 （mean 10.9 ± 3.7） years. On intent-to-treat analysis （n = 38）, the eradication rate of H pylori was 73.7% （95% CI, 65.2%-82%） whereas in per-protocol analysis （n = 33） it was 84.8% （95% CI, 78.5%-91%）. All the patients with duodenal ulcer （n = 7） were successfully treated （100% vs 56.2% with antral nodularity）. Side effects were reported in 26 patients （68.4%）, mainly vomiting （14/26） and abdominal pain （n = 13）. Successfully treated dyspeptic patients showed improvement in 78.9% of H pylori-negative patients after six months and in 50% of Hpylori-positive patients after six months of treatment. CONCLUSION： Triple therapy with furazolidone achieves moderate efficacy in H pylori treatment. The eradication rate seems to be higher in patients with duodenal ulcer.

关键词： Furazolidone H pylori treatment Gastritis Children

The impact of ancestral population size and incomplete lineage sorting on Bayesian estimation of species divergence times

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Current Zoology 2015年第5期61卷 874-885页

作者： Konstantinos ANGELIS Mario DOS reis Department of Genetics Evolution and Environment University College London Gower Street London WC 1E 6BT UK

Although the effects of the coalescent process on sequence divergence and genealogies are well understood, the vir- tual majority of studies that use molecular sequences to estimate times of divergence among species have failed to account for the coalescent process. Here we study the impact of ancestral population size and incomplete lineage sorting on Bayesian estimates of species divergence times under the molecular clock when the inference model ignores the coalescent process. Using a combination of mathematical analysis, computer simulations and analysis of real data, we find that the errors on estimates of times and the molecular rate can be substantial when ancestral populations are large and when there is substantial incomplete lineage sorting. For example, in a simple three-species case, we find that if the most precise fossil calibration is placed on the root of the phylogeny, the age of the internal node is overestimated, while if the most precise calibration is placed on the internal node, then the age of the root is underestimated. In both cases, the molecular rate is overestimated. Using simulations on a phylogeny of nine species, we show that substantial errors in time and rate estimates can be obtained even when dating ancient divergence events. We analyse the hominoid phylogeny and show that estimates of the neutral mutation rate obtained while ignoring the coalescent are too high. Using a coalescent-based technique to obtain geological times of divergence, we obtain estimates of the mutation rate that are within experimental estimates and we also obtain substantially older divergence times within the phylogeny [Current Zoology 61 （5）： 874-885, 2015].

关键词： Ancestral polymorphism Incomplete lineage sorting Divergence time estimation Gene tree Species tree

Analyzing the economic viability of a large-scale integrated model of municipal solid waste:a study case for the most important Brazilian economic region

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Waste Disposal and Sustainable Energy 2020年第3期2卷 231-247页

作者： Octavio Pimenta reis Neto Mechanical Engineering Faculty at State University of Campinas(PDSE/FEM/UNICAMP) Av.Mofarrej275-Apto 212 B1São PauloSP CEP 05311-000Brazil

Despite the National Policy for Solid Waste(PNRS)in 2010,nothing has changed to waste disposal in *** to reach 100%of all Municipal Solid Waste(MSW)collected and treated in landfills by Aug.2nd,2014,until nowadays,42%of this total remains in *** the most important national economic region treating its urban waste in landfills,what it has is no more than 4%of recycling and its landfills reaching the *** other ones is getting harder year by year,due to water reservoirs around the region,high freight costs,waste disposal and the severe control of emissions associated with its *** article comes to break the paradigm of investment and profitability proposing an alternative to the land-use,achieving higher rates of *** economic viability,carried out through well-known financial variables and Monte Carlo analysis,has taken into account proven local waste characteristics and market prices for recyclables and energy without *** considered a proposal highly intensive in capital and people,the revenues from the sales would be enough to guarantee the viability of 100%equity with IRR of 33.7%and ROI of 24.5%per year within the confidence of 99%.

关键词： Solid waste Sorting Recycling Waste-to-energy Metropolitan Region of São Paulo Energy recovering

Exploring spatial relationships between stream channel features,water depths and flow velocities during flash floods using HEC-GeoRAS and Geographic Information Systems

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Journal of Geographical Sciences 2022年第4期32卷 757-782页

作者： Miguel LEAL Eusébio reis Pedro Pinto SANTOS Centre of Geographical Studies and Associated Laboratory TERRA Institute of Geography and Spatial PlanningUniversidade de Lisboa1600-276 LisbonPortugal Forest Research Centre and Associated Laboratory TERRA School of AgricultureUniversidade de LisboaTapada da Ajuda1349-017 LisbonPortugal

Water depths and flow velocities decisively influence the damage caused by flash *** Information System(GIS)is a powerful and useful tool,allowing the spatial analysis of results obtained by hydraulic modelling,namely from the HEC-RAS/HEC-GeoRAS *** GIS spatial analysis performed in this study seeks to explain and quantify the spatial relationships between the stream channel features and flow components during flash flood *** these relationships are generically known,there are few studies exploring this subject in different geographic contexts.A 1D hydraulic model was applied in a small watershed in Portugal,providing good results in the definition of floodable areas,water depths and longitudinal *** direct relationship was found between water depths and velocities in the floodable areas;however,negative strong correlations were found between the two flow components along the stream *** slope,channel and flood width,and roughness prove to be highly relevant on the longitudinal variations of water depths and velocities and on the location of maximum *** peak discharges and return periods(R;)can change the relationships between water depths and velocities at the same *** can be improved with more accurate elevation data for stream channels and floodplains.

关键词： flash floods water depth flow velocity geographic information systems(GIS) spatial analysis

Maternal hyperthyroidism increases the synthesis activity and the osteogenic markers expression of calvarial osteoblasts from offspring in a murine model

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BIOCELL 2023年第2期47卷 423-430页

作者： FABIANA ROCHA ARAÚJO BRUNO machado BERTASSOLI ISABELLA CRISTINA SOUZA FÉLIX DOUGLAS MARINHO ABREU NATÁLIA MELO OCARINO AMANDA MARIA SENA reis JUNEO FREITAS SILVA ROGÉRIA SERAKIDES Núcleo de Células Tronco e Terapia Celular Animal(NCT-TCA) Veterinay SchoolUniversidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil Universidade de Uberaba(UNIUBE) UberabaMinas Gerais38065-300Brasil Instituto de Ciências Biológicas Universidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil Universidade Estadual de Santa Cruz Campus Soane Nazare de AndradeIlheusBahiaBrasil

To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal *** adult Wistar rats were divided into two groups,one control and one treated with daily administration of L-thyroxine by an orogastric tube(50μg/animal/day)during *** days after delivery and confirmation of the mothers’hyperthyroidism,the offspring were euthanized for the extraction of osteoblasts from the *** 7,14,and 21 days,proliferation activity was assessed using MTT assay,while alkaline phosphatase(ALP)activity was assessed by the BCIP/NBT *** 21 days,the total area of the mineralized matrix stained by von Kossa was evaluated by *** expression of gene transcripts for Runx2,Bmp2,Fgfr1,collagen type 1(Col1),osteocalcin(Oc),and osteopontin(Op)were evaluated by real-time *** were compared using the Student’s *** activity was significantly higher at 14 and 21 days in cultures of osteoblasts extracted from offspring exposed to maternal hyperthyroidism,while MTT conversion was significantly lower at 21 days in this *** cultures of neonates exposed to maternal hyperthyroidism also showed a larger total area of mineralized matrix and greater expression of gene transcripts for Oc and *** hyperthyroidism increases the activity of matrix synthesis,alkaline phosphatase activity,and expression of gene transcripts for osteocalcin and osteopontin in the osteoblasts,extracted from the calvaria of the offspring,which may be one of the mechanisms of premature fusion of cranial sutures.

关键词： Craniosynostosis Intramembranous growth Ossification Rat Thyroid dysfunction