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Preparation of Reference Material for Proficiency Test for Enumeration of Coliforms in Cheese Matrix

detection 2013年第1期1卷 7-12页

作者： Marcelo Luiz Lima Brandao Carla de Oliveira Rosas Silvia Maria Lopes Bricio Valeria de Mello Medeiros juliana de castro beltrao da costa Rodrigo Rollin Pinheiro Paola Cardarelli-Leite Marcus Henrique Campino de La Cruz Armi Wanderley da Nobrega Laboratory of Products Department of MicrobiologyNational Institute of Quality Control in HealthOswaldo Cruz FoundationRio de JaneiroBrazil Proficiency Testing Program of Products Subject to Health Surveillance National Institute of Quality Control in HealthOswaldo Cruz FoundationRio de JaneiroBrazil

It is widely accepted that quantitative reference materials (RM), are indispensable tools for verification of the precision and accuracy of analytical measurements. The RM can be used by food microbiology laboratories, as part of their quality assurance programmes, to achieve their quality control. In Brazil, Anvisa RDC No. 12/01 specifies the enumeration of coliforms as one of the parameters for evaluating cheese quality. The aim of this study was to produce a quantitative RM for proficiency testing (PT) for use in the testing of enumeration of coliforms in cheese matrixes. A sample of an ultra-filtered cheese with a coliforms count of dered sufficiently homogeneous and stable at ≤ ?70?C during the entire study period (348 days). The material was also considered sufficiently stable at 4?C for six days, but instable at 30?C and 35?C for the same period. At ?20?C the RM was sufficiently stable for 161 days. It was concluded that the material showed all the necessary requirements for a quality RM to be used as PT items and could be transported to the laboratories taking part in a PT at up to 4?C for up to 6 days, since the results indicated maintenance of the cell concentrations during this period. This is the first study to describe a methodology for producing RM containing coliforms in a cheese matrix.

关键词： Coliforms Cheese Reference Material Proficiency Test

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King's College criteria and the Clichy-Villejuif criteria require adjustments for assessing acute liver failure due to yellow fever

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World Journal of Transplantation 2025年第1期15卷 124-134页

作者： Bruno da Silva Athanasio Antonio Marcio de Faria Andrade Vivian Vasconcelos costa Juliano Felix castro Silverio Leonardo Macedo Garcia Mauro Martins Teixeira daniele da Gloria Souza Paula Vieira Teixeira Vidigal Cristiano Xavier Lima department of Surgery Federal University of Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Liver Transplantation Unit Felicio Rocho HospitalBelo Horizonte 30110-934Minas GeraisBrazil department of Morphology Federal University of Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil department of Biochemistry and Immunology Federal University of Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil department of Pathology Federal University of Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil

BACKGROUND Acute liver failure(ALF)is a severe condition characterized by rapid deterioration of liver function in individuals without preexisting liver *** transplantation(LT)is the most impactful *** fever(YF)is an infectious disease that primarily affects the liver and has a high mortality ***,LT can be a viable option for treating rare cases with extensive liver ***,the criteria for assessing the severity of ALF and determining the indications for transplantation have not been specifically validated for cases caused by *** To present necessary adjustments to established scoring systems for ALF secondary to *** This was an observational,retrospective,single-center *** consecutive patients with confirmed ALF due to YF were monitored in the intensive care unit by a specialized liver transplant team during a three-month epidemic outbreak in *** hospitalization,general supportive therapeutic measures were implemented,and the patients were regularly assessed using the King's College criteria and the Clichy-Villejuif criteria to determine the severity of liver *** is considered a viable measure for patients with signs of end-stage liver *** Eight of 14(57%)patients developed severe neurological alterations within the first 96 hours after hospital *** patients underwent emergency LT,and despite a moderate viral infection of the graft after transplantation,the 5-year survival rate was 50%.Although the King's College criteria and the Clichy-Villejuif criteria are the main scoring systems for ALF,they are insufficient for predicting the risk of mortality in this context,primarily because of low serum bilirubin levels in the final stage of the disease and significant disparities between coagulation abnormalities and patient *** To ensure good applicability in cases of YF-induced ALF,the authors suggest adaptations to the King's College and Clichy-Vill

关键词： Yellow fever Acute liver failure Liver transplantation King's College criteria Clichy-Villejuif criteria

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Osmoregulators in Hymenaea courbaril and Hymenaea stigonocarpa under water stress and rehydration

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Journal of Forestry Research 2018年第6期29卷 1475-1479页

作者： Luma castro de Souza Luana Moraes da Luz Je′ssica Taynara da Silva Martins Candido Ferreira de Oliveira Neto Juscelino Goncalves Palheta Tamires Borges de Oliveira Ediane Conceicao Alves Risely Ferraz de Almeida Raimundo Leonardo Lima de Oliveira Roberto Cezar Lobo da costa Nariane Quaresma Vilhena Universidade estadual paulista ju′lio de mesquita filho JaboticabalSP 14884-900Brazil Universidade Federal Rural da Amazonia(UFRA) Campus CapanemaCapanema 68700-030Brazil Universidade Federal Rural da Amazonia Campus BelemBelem 66077-830Brazil Universidade Federal de Vicosa(UFV) Vicosa 36570-900Brazil Universidade Estadual Paulista Ju′lio de Mesquita Fiho(UNESP) Jaboticabal 14884-900Brazil Universidade do Estado do Para′(UEPA) Bele′m 66113-200Brazil

The objective of this work was to evaluate the effect of different water deficiency and rehydration levels on the concentrations of osmoregulators in two plant species（Hymenaea courbaril and H. Stigonocarpa） in the Amazon. We adopted a 2×5×5 factorial system,referring to 2 species（H. courbaril and H. stigonocarpa）and 5 stages of hydration and rehydration. The five hydration and rehydration stages were established in:（1）Control treatment E0;（2） Plants with 13 days of stress after incubation—E13;（3） Plants with 26 days of stress E26;（4）The plants that were established after 26 days after incubation and rehydrated for two days（RD2）;（5） rehydrated for two days（RD4）. The plants that were established after 26 days after incubation and rehydrated for four days. The experiment totaled fifty young plants with five *** measurements were performed at the beginning of the experiment（E0） at 13（E13） and 26（E26） days after the water stress, in which the plants were rehydrated,repeating the analyses after two（RD2） and four（RD4）days. Both species increased the sucrose concentration by18%, with a decrease of 52% in starch content. The RD4 time presented the highest mean starch concentration(0.19 mmol g-1 of the residue for H. courbaril and0.27 mmol g;of residue for H. stigonocarpa). Increased proline concentrations were recorded for controls until RD2 for both species. For glycine betaine, the highest increases in treatments E26 and RD2 were observed for the H. courbaril species. Our rehydration period was not sufficient for total recovery of pre-stress concentrations of all studied solutes.

关键词： Amazonia Water stress Osmotic adjustment Proline Sucrose

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Pseudomelanosis duodeni associated with chronic renal failure

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World Journal of Gastroenterology 2012年第12期18卷 1414-1416页

作者： Marcia Henriques de Magalhes costa Maria da Gloria Fernandes Pegado Cleber Vargas Maria Elizabeth C castro Kalil Madi Tiago Nunes Cyrla Zaltman Gastroenterology Section Department of Internal MedicineClementino Fraga Filho University HospitalUniversidade Federal do Rio de JaneiroRio de Janeiro 21941-913Brazil department of Pathology Clementino Fraga Filho University HospitalUniversidade Federal do Rio de JaneiroRio de Janeiro 21941-913Brazil Hospital Clínic i Provincial/IDIBAPS Barcelona 08036Spain

Pseudomelanosis duodeni (PD) is a rare dark speckled appearance of the duodenum associated with gastrointestinal bleeding,hypertension,chronic heart failure,chronic renal failure and consumption of different *** report four cases of PD associated with chronic renal failure admitted to the gastroenterology outpatient unit due to epigastric pain,nausea,melena and progressive reduction of hemoglobin *** endoscopy revealed erosions in the esophagus and stomach,with no active bleeding at the *** addition,the duodenal mucosa presented marked signs of melanosis;later confirmed by histopathological *** though PD is usually regarded as a benign condition,its pathogenesis and clinical significance is yet to be defined.

关键词： Pseudomelanosis duodeni Chronic renal fail-ure Diabetes mellitus Anti hypertensive drugs Gastro-duodenal endoscopy

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Predictive modeling for wine authenticity using a machine learning approach

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Artificial Intelligence in Agriculture 2021年第1期5卷 157-162页

作者： Nattane Luíza da costa Leonardo A.Valentin Inar Alves castro Rommel Melgaço Barbos Instituto de Informática Universidade Federal de GoiásGoiânia-GoBrazil Informatics Nucleo Goiano Federal Institute of EducationScience and TechnologyCampus UrutaíRodovia Geraldo Silva Nascimentokm 2.5Zona RuralBloco de InformáticaUrutaíGOBrazil LAdaF Department of Food and Experimental NutritionFaculty of Pharmaceutical SciencesUniversity of São PauloAv.Lineu Prestes580B1405508-900 São PauloBrazil

The purpose of this paper is to classify wines from 4 different countries in South *** class of wines is formed by samples considered by experts as representatives of the following commercial categories:“Argentinean Malbec(AM)”,“Brazilian Merlot(BM)”,“Uruguayan Tannat(UT)”and“Chilean Carménère(CC)”.The 83 samples collected were analyzed according to their composition of volatiles,semi-volatiles and phenolic *** built a decision system for classification based on support vector machines(SVM),along with Correlation-based Feature selection(CFS),and RandomForest Importance(RFI),whichmeasures the relative importance of the input ***,we use CFS to select a subset of variables among 190 chemical *** chemicals were selected as correlated to the category and uncorrelated with each ***,these chemical compounds were organized according to the importance ranking given by the RFI and classified with *** study clearly indicated that SVMin combination with feature selection methodswas able to identify the most important chemicals to classify the wine *** the compounds identified in the wine samples,the variable subset defined by the feature selection methods,which were catechin,gallic,octanoic acid,myricetin,caffeic,isobutanol,resveratrol,kaempferol,and ORAC,were able to achieve an accuracy of 93.97%in classifying the commercial categories.

关键词： Wine classification Support vector machines Feature selection South American wines Machine learning

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Effect of applying a calcined kaolin-based particle film on the photosynthetic capacity and growth of young eucalyptus plants

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Journal of Forestry Research 2021年第6期32卷 2473-2484页

作者： Diego Pereira Santos Mauriana da Rocha Sobrinho Maria de Fatima de castro Oliveira Niedja Bezerra costa Tiago Massi Ferraz Fabricio de Oliveira Reis Heder Braum Eliemar Campostrini Fabio Afonso Mazzei Moura de Assis Figueiredo Graduate Program in Agriculture and Environment State University of MaranhaoPraca Goncalves Diass/nCentroBalsasMA 65800-000Brazil Graduate Program in Agroecology State University of MaranhaoAv.Lourenco Vieira da Silva1000Jardim Sao CristovaoSao LuisMA 65055-310Brazil Graduate Program in Agronomy Campus SamambaiaEscola de AgronomiaFederal University of GoiasAv.Esperanca s/nGoianiaGO 74690-900Brazil State University of Maranhao Av.Lourenco Vieira da Silva1000Jardim Sao CristovaoSao LuisMA 65055-310Brazil Northern Rio de Janeiro State University Av.Alberto Lamego2000Campos dos GoytacazesRJ 28013-602Brazil

In scenarios of climatic change when increased global temperatures can be expected,it is essential to search for technologies that favor sapling survival and growth after planting and increase yield in the ***-based particle films(KBPF)have been applied as barriers against the deleterious effects of high levels of solar *** objective of the present study was to assess the effects of applying purified calcined kaolin-based particle film to young eucalyptus *** treatments were carried out:0%(just water),3%,5%,7%,and 10%calcined kaolin applied to the adaxial part of the leaves.A complete randomized block design was used with five treatments and ten *** plants were assessed for height,diameter at ground level,gas exchanges(net photosynthetic CO_(2) assimilation,stomatal conductance and transpiration),instantaneous(WUE)and intrinsic water use efficiency(IWUE),chlorophyll a fluorescence(maximum quantum yield of photosystem II(PSII)(Fv/Fm),concentration of active reaction centers in relation to the quantity of photons absorbed(RC/ABS)and the maximum ratio of quantum yields of photochemical and concurrent non-photochemical processes in PSII(Fv/F0),SPAD reading and leaf *** 3%KBPF concentration showed the best responses in biometric assessments 80 days after planting(daP)and were corroborated by the responses of the leaf,stem and shoot dry matter production as a *** leaf ontogeny assessments showed positive responses following KBPF application when considering leaf development,with 7%KBPF concentration resulting in the highest mean *** mean specific leaf mass had negative response to high KBPF *** 60 daP,the gas exchange variables during both assessment periods declined with an increase in KBPF *** differences as a result of KBPF applications were found only at the start of the assessments(34 daP)for both WUE and for ***‘greenness’(SPAD reading)at 47 days showed a quadratic

关键词： Ecophysiology Photoprotection Yield Silviculture

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Latent tuberculosis:Risk factors,screening and treatment in liver transplantation recipients from an endemic area

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World Journal of Transplantation 2021年第12期11卷 512-522页

作者： Isabela Dias Lauar Luciana costa Faria Roberta Maia de castro Romanelli Wanessa Trindade Clemente Medicine department Universidade Josédo Rosário VellanoBelo Horizonte 31710030Minas GeraisBrazil Internal Medicine department Faculdade de MedicinaUniversidade Federal de Minas Gerais(UFMG)Belo Horizonte 30130100Minas GeraisBrazil Pediatrics department Faculdade de MedicinaUniversidade Federal de Minas Gerais(UFMG)Belo Horizonte 30130100Minas GeraisBrazil department of Laboratory Medicine Faculdade de MedicinaUniversidade Federal de Minas Gerais(UFMG)Belo Horizonte 30130100Minas GeraisBrazil

BACKGROUND Patients undergoing solid organ transplantation,particularly those who live or have lived in tuberculosis(TB)endemic areas,are at a high risk of developing *** majority of post-transplantation TB cases are associated with reactivation of latent TB infection(LTBI).Brazil is in a single position with overlapping areas of high TB endemicity and high transplant *** liver transplant(LT),one should be aware of the potential hepatotoxicity associated with the treatment regimens for *** To evaluate the frequency of LTBI in LT patients and treatment-related *** This was a retrospective analysis of a cohort of cirrhotic patients aged≥18 years,who underwent LT at a high-complexity teaching hospital from January 2005 to december *** Overall,429 patients underwent LT during the study *** these,213(49.7%)underwent the tuberculin skin test(TST)during the pre-transplant period,and 35(16.4%)of them had a positive *** treatment for LTBI was initiated after LT in 12(34.3%)of the TST-positive patients;in 3(25.0%),treatment was maintained for at least 6 *** The prevalence of LTBI was lower than *** and completion of LTBI treatment was limited by difficulties in the management of these special patients.

关键词： Latent tuberculosis Liver transplantation Tuberculosis Infection Transplantation Risk factors

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Hydrodynamics and suspended particulate matter retention in macrotidal estuaries located in Amazonia-semiarid interface(Northeastern-Brazil)

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International Journal of Sediment Research 2020年第4期35卷 417-429页

作者： Vinicius Henrique Maciel dos Santos Francisco Jose da Silva Dias Audalio Rebelo Torres Romulo Araujo Soares Lais costa Terto Antonio Carlos Leal de castro Ricardo Luvizotto Santos Marco Valerio Jansen Cutrim Laboratorio de Hidrodinamica Costeira Estuarina e de Aguas Interiores (LHiCEAI) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA)Cidade Universitaria Dom Delgado Laboratorio de Ictiologia (LabIctio) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado Laboratorio de Ecotoxicologia (LabEcotox) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado Laboratorio de Ficologia (LabFic) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado

The aim of the current study was to determine the nature of the seasonal variability of the Suspended Particulate Matter(SPM) fluxes from the drainage basin to the estuary in a macrotidal region(Northeastern Brazil),and the estuarine response to a seawater intrusion regarding sediment deposition,which will support the understanding of the global transport of materials at the continent-ocean *** structure data was acquired using a Conductivity,Temperature,and depth(CTD) probe with a sampling frequency of 4 *** particulate material was measured by gravimetric measurements applied to exact filtered volume *** outflows were measured through the use of an Acoustic Doppler Current Profiler(ADCP) with frequency of 1.5 *** horizontal thermal and saline gradients varied from warmer and less saline waters(2014) to cooler and saline waters(2015).The gradient behavior when linked to volume transport and SPM flows,suggests a minimization of the fluvial flows in 2015,easing the advance of coastal water(CW) towards the inner estuary,leading to an inversion of the baroclinic pressure *** bottom saline front,generated by the entrance of coastal water masses,caused an increase in SPM concentrations due to increased fluid density,resuspension of previously deposited sediment,and erosion of *** concentrations of SPM indicate higher volume transport suggesting a hydraulic barrier due to the change/inversion of the baroclinic pressure gradient,resulting in water and material *** deposition was observed during neap tide,while during spring tide the material is resuspended,increasing the concentration,generating cycles of deposition and erosion during the neap-spring *** sediment in suspension that reach the estuary,even with low fluvial volume,stay in this environment forming new islands because of *** deposition rates or sediment cycling,if generated by the hydraulic barrier,may indicate that the flo

关键词： Brazilian Amazonia Materials discharges Macrotidal estuaries Freshwater percentage Residence time

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Carbon, Water, Ecological Footprints, Energy and Nutritional densities of Omnivore and Vegan Culinary Preparations

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Food and Nutrition Sciences 2023年第7期14卷 626-637页

作者： Thadia Turon costa da Silva Bianca Biscacio Falco Isis Gomes de castro Rita Barreira Zanon juliana Vidal Vieira Guerra Karina Yuriko Yaginuma Verônica Oliveira Aline Gomes de Mello de Oliveira department of Nutrition and Dietetics Josué de Castro Nutrition Institute Federal University of Rio de Janeiro Rio de Janeiro Brasil Math and Statistics Institute Fluminense Federal University Niterói Brasil

This study aimed to evaluate energy and nutritional densities, water, carbon and ecological footprints, cost, of omnivorous and vegan main courses served in a university restaurant in the city of Rio de Janeiro. A cross-sectional, analytical study was conducted with 40 main dish-type preparations, 20 of which are omnivorous and 20 vegans, served at lunch, to assess energy and nutritional densities, carbon, water and ecological footprints and cost of preparations. We propose a healthy and sustainable preparation index (HSPI) to evaluate from the list, the best preparation options considering the nutritional quality combined with the impact that the food causes on the environment. Preparations with the highest HSPI were considered the best options because they have a good relation between the nutritional profile and the environmental impact. Results: Regarding energy (ED), nutritional (ND) densities and water, carbon and ecological footprints, omnivorous preparations presented much higher values when compared to the vegan ones. The omnivorous preparations had the highest average cost (R$ 3.44). Regarding the HSPI, vegan preparations showed better rates than omnivorous preparations. Food services should promote healthy and sustainable choices by offering menus with low energy density preparations, high nutritional density, and low environmental impact, considering local realities and customer needs. Conclusion: This study was able to evaluate the best preparation options, considering the nutritional profile and the food impact on the environment, using health and sustainable indicators. Obtaining indicators of preparations regarding healthiness and sustainability, in practice, translates environmental aspects in menu planning, which contribute to changes in food consumption patterns in food services, in addition to contributing to the reduction of the environmental impact. In this way, they can be used as tools added to the menu planning process for the analysis of the

关键词： Food Services Menu Planning Environment Carbon Footprint Water Footprint

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Biquinho pepper(Capsium chinense):Bioactive compounds,in vivo and in vitro antioxidant capacities and anti-cholesterol oxidation kinetics in fish balls during frozen storage

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Food Bioscience 2022年第3期47卷 512-523页

作者： Bárbara Jardim Mariano Vanessa Sales de Oliveira davy William Hidalgo Chávez Rosane Nora castro Cristiano Jorge Riger Jéssica Sodré Mendes Marcelo da costa Souza Alexandra Christine Helena Frankland Sawaya Geni Rodrigues Sampaio Elizabeth Aparecida Ferraz da Silva Torres Tatiana Saldanha department of Food Technology Institute of TechnologyUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil department of Chemistry Institute of ChemistryUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil department of Biochemistry Institute of ChemistryUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil department of Botany Institute of Biological Sciences and HealthUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil Faculty of Pharmaceutical Science University of CampinasUNICAMPCampinasSPBrazil department of Nutrition School of Public HealthUniversity of São Paulo (USP)Ave.Dr.Arnaldo715São PauloSP01246-904Brazil

The present study reports bioactive constituents from biquinho pepper,such as carotenoids,capsaicinoids,and capsinoids,identified by *** pepper extract showed an antioxidant potential by DPPH(33.17±0.08%),ORAC(32.35±0.02μM TE/g),β-carotene/linoleic acid(55.80±1.30%),and FRAP(315.88±4.23μmol TE/g)***,in vivo antioxidant analyses demonstrated that the pepper extract(170μg/mL)decreased the damage promoted by H_(2)O_(2) in Saccharomyces cerevisiae *** balls were prepared using different concentrations of pepper(0.0%,0.5%,0.75%,1.0%)to investigate its antioxidant effect during frozen *** pepper effectively reduced the formation of cholesterol oxides and preserved essential fatty acids,mainly in samples containing 1.0%of ***,this treatment was more efficient than sodium erythorbate(pdation was also determined,showing that both cholesterol degradation and cholesterol oxides formation fit a first-order kinetic model(R^(2)>0.8).

关键词： Bioactive compounds Capsicum chinense Cholesterol oxides Frozen storage Natural antioxidants Unsaturated lipids

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