检索结果-南通市图书馆

Site-directed cysteine coupling of disulfide-containing non-antibody carrier proteins(THiOCAPs)

Science China Materials 2023年第10期66卷 4109-4120页

作者： Ariana Rueda julian i.mendoza Lorena Alba-Castellon Eloi Parladé Eric Voltà-Durán David Paez Anna Avino Ramon Eritja Esther Vázquez Antonio Villaverde Ramón Mangues Ugutz Unzueta institut d'investigació Biomèdica Sant Pau(iiB Sant Pau) Sant Quintí 77-7908041 BarcelonaSpain Josep Carreras Leukaemia Research institute(iJC Campus Sant Pau) 08041 BarcelonaSpain CiBER de Bioingeniería Biomateriales y NanomedicinaInstituto de Salud Carlos Ⅲ28029 MadridSpain institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona08193 BellaterraSpain Departament de Genètica i de Microbiologia Universitat Autònoma de Barcelona08193 BellaterraSpain Department of Medical Oncology Hospital de la Santa Creu i Sant Pau.Barcelona08025 BarcelonaSpain CiBER de Enfermedades Raras(CiBERER) Instituto de Salud Carlos Ⅲ28029 MadridSpain institute for Advanced Chemistry of Catalonia(iQAC) CSIC08034 BarcelonaSpain

The development of a new generation of nonantibody protein drug delivery systems requires site-directed conjugation strategies to produce homogeneous,reproducible and scalable *** that,the genetic addition of cysteine residues into solvent-exposed positions allows the thiol-mediated cysteine coupling of therapeutic drugs into protein-based ***,the high reactivity of unpaired cysteine residues usually reduces protein stability,consequently imposing the use of more methodologically demanding purification *** is especially relevant for disulfide-containing nanocarriers,as previously observed in ***,although many protein scaffolds and targeting ligands are also rich in disulfide bridges,the use of these methodologies over emerging non-antibody carrier proteins has been completely ***,we report the development of a simple and straightforward procedure for a onestep production and site-directed cysteine conjugation of disulfide-containing non-antibody thiolated carrier proteins(THiOCAPs).This method is validated in a fluorescent C-X-C chemokine receptor 4(CXCR4)-targeted multivalent nanocarrier containing two intramolecular disulfide bridges and one reactive cysteine residue strategically placed into a solvent-exposed position(THiO-T22-GFP-H6)for drug conjugation and in a humanized alternative intended for clinical applications(T22-HSNBT-H6).Thus,we produce very stable,homogeneous and fully functional antitumoral nanoconjugates(THiO-T22-GFP-H6-MMAE and T22-HSNBT-H6-MMAE)that selectively eliminate target cancer cells via ***,the developed methodology appears as a powerful tool for the rational engineering of emerging non-antibody,cell-targeted protein nanocarriers that contain disulfide bridges together with a solvent-exposed reactive cysteine(THiOCAP).This should pave the way for the development of a new generation of stable,homogeneous and efficient nanomedicines.

关键词： disulfide directed bridges

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

dentification of AtOPT4 as a Plant Glutathione ;ransporter

引用

Molecular Plant 2016年第3期9卷 481-484页

作者： Zhongchun Zhang Qingqing Xie Timothy O. Jobe Andrew R. Kau Cun Wang Yunxia Li Baosheng Qiu Qiuquan Wang David G. mendoza-Cdzatl julian i. Schroeder Division of Biological Sciences Cell and Developmental Biology Section University of California San Diego La Jolla CA 92093-0116 USA School of Life Sciences Central China Normal University Wuhan 430079 Hubei P.R. China Hubei Key Laboratory of Genetic Regulation and integrative Biology Central China Normal University Wuhan 430079 Hubei P.R. China Department of Chemistry & the MOE Key Lab of Spectrochemical Analysis and instrumentation College of Chemistry and Chemical Engineering Xiamen University Xiamen Fujian 361005 China Division of Plant Sciences C.S. Bond Life Sciences Center University of Missouri Columbia MO 65211 USA

Glutathione （GSH） is an essential tri-peptide important for several plant processes, including the control of cellular redox status, detoxification of xenobiotics, root development, heavy metal transport and resista... 详细信息

关键词： detoxification Cadmium phytochelatins

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：