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RNA INTERFERENCE OF ANneXIN II gEne IN PC3 CELLS BY USINg SMALL INTERFERENCE RNA SYNTHESIZED WITH IN VITRO TRANSCRIPTION

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Chinese Medical Sciences Journal 2007年第1期22卷 33-37页

作者： ya-wei Yuan Ai-min Sun Ying Lui Long-hua Chen Banerjee A. g Department of Radiotherapy Nanfang Hospital of Nanfang Medical University Guangzhou 510515 Department of Biochemistry and Molecular Biology University. of Nebraska Medical Center Omaha NE 68198-7835 USA

Objective To silence annexin Ⅱ gene expression by using small interference RNA （siRNA） in prostate cancer cell line PC3. Methods For in vitro transcription, four sequences of 29-nucleotide DNA template oligonucleotides were designed, and one pair of the sequences were complementary to annexin Ⅱ gene. The other pair was negative control. The 8 nucleotides at the 3＇ end of each oligonucleotide were complementary to the T7 Promoter Primer. The sense and anti-sense siRNA templates were transcribed by T7 RNA polymerase and the resulting RNA transcripts were hybridized to create dsRNA. The siRNA was transfected into prostate cancer cell PC3. For assaying the efficiency of siRNA, confocal microscopy, Northern blotting, and Western blotting were employed to examine the expression of annexin Ⅱ protein and its mRNA. ^3H thymidine was used to measure DNA synthesis. Results The siRNA sequence specific to annexin Ⅱ gene was capable of inhibiting the expression of annexin Ⅱ protein and its mRNA. And cellular DNA synthesis was significantly reduced in siRNA transfected cells.Conclusions The protocol for the synthesis of siRNA by T7 RNA polymerase is feasible. Annexin Ⅱ might be involved in DNA synthesis.

关键词： small interference RNA gene silencing annexin Ⅱ prostate cancer cell

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类人胶原蛋白基因工程菌生长期动力学模型

化学工程 2008年第12期36卷 46-49,78页

作者：骆艳娥范代娣迟雷西北大学化工学院陕西西安710068

研究类人胶原蛋白基因工程菌生长期动力学模型,为优化发酵调控提供理论依据和指导。应用元素衡算和代谢衡算(EMB)方法,建立类人胶原蛋白基因工程菌生长期的动力学模型,并利用线性规划法对动力学模型参数αh和mX进行估算。结果表明,αh... 详细信息

研究类人胶原蛋白基因工程菌生长期动力学模型,为优化发酵调控提供理论依据和指导。应用元素衡算和代谢衡算(EMB)方法,建立类人胶原蛋白基因工程菌生长期的动力学模型,并利用线性规划法对动力学模型参数αh和mX进行估算。结果表明,αh和mX分别为1.173 mol/C-mol和2.326 mol/(C-mol.h),该模型能较好地预测重组大肠杆菌生长期的宏观反应速率,且确立了葡萄糖的流加方程。利用EMB方法建立的动力学模型具有重要的应用价值,若要进一步减小氮消耗速率和CO2产生速率预测误差,需要对重组大肠杆菌的代谢网络进行系统分析,建立该模型的修正系数。

关键词：元素衡算代谢衡算重组大肠杆菌类人胶原蛋白动力学模型

neuroendocrine cells of prostate cancer: biologic functions and molecular mechanisms

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Asian Journal of Andrology 2019年第3期21卷 291-295页

作者： Yu-Hua Huang ya-Qun Zhang Jiao-Ti Huang Department of Urology The First Affiliated Hospital of Soochow University Suzhou 215006 China Department of Urology Beijing Hospital National Center of Gerontology Beijing 100730 China Department of Pathology Duke University School of Medicine Durham NC 27514 USA

Prostate cancer (PCa) is a major health risk for older men worldwide. Existing systemic therapies mostly target androgen receptor (AR). Although treatments are initially effective, the disease always recurs. A potential mechanism for the treatment failure is that PCa contains, in addition to the AR-positive luminal type tumor cells, a small component of neuroendocrine (ne) cells. The function of ne cells in PCa remains poorly understood, and one important characteristic of these cells is their lack of expression of AR and resistance to hormonal therapy. In addition, many patients develop the more aggressive small-cell neuroendocrine carcinoma (SCNC) after hormonal therapy. Although this clinical phenomenon of disease transformation from adenocarcinoma to SCNC is well established, the cell of origin for SCNC remains unclear. Recently, loss of function of Rb and TP53 and amplification and overexpression of MYCN and Aurora A kinase have been identified as important biomarkers and potential disease drivers. In this article, we systematically review the histology of normal prostate and prostate cancer including the main histologic types: adenocarcinoma and SCNC. We also review the findings from many studies using cellular and animal models as well as human specimens that attempt to understand the molecular mechanisms of treatment failure, disease progression, and tumor transformation from adenocarcinoma to SCNC.

关键词： adenocarcinoma neuroendocrine prostate small-cell carcinoma

Liquid Crystalline Microdroplets of graphene Oxide via Microfluidics

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Chinese Journal of Polymer Science 2021年第12期39卷 1657-1664页

作者： Piao Ma Peng Li ya Wang Dan Chang Wei-Wei gao Chao gao MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringKey Laboratory of Adsorption and Separation Materials Technologies of Zhejiang ProvinceZhejiang UniversityHangzhou 310027China

Study of stable liquid crystal(LC)microdroplets is of great significance for LC dynamics in con fined space or at topological ***,the fabrication of LC microdroplets with diverse shape without ionic gelati on agents still remai ns challe nging due to the fluid ***,we utilize the microfluidic tech no logy to prepare graphe ne oxide(gO)LC microdroplets with various morphologies based on the anomalous rheological property of gO aqueous *** from LC of one-dimensional polymer,LC containing two-dimensional gO sheets exhibits considerable viscoelasticity and weak extensibility,resulting from the planar molecular conformation and the absence of intermolecular entangleme *** low exte risibility ensures that gO aqueous suspension is discretized into mono dispersed microdroplets rather than thin thread in the microfluidic *** large viscoelasticity and ultra-1 ong relaxation time of gO LC enable the diverse stable morphologies of *** droplet morphology is well con trolled from sphere to teardrop by modulati ng the competition between gO viscoelasticity and in terfacial ten *** two-dimensional gO LC featuri ng unique rheological property provides a novel system for the microfluidic field,and corresponding topological stability enriches the LC dyn amics and ope ns a new pathway for desig ning graphe ne-based materials.

关键词： graphene oxide Liquid crystal Microdroplets Microfluidics

Efficient use of waste heat and solar energy： Technologies o cooling, heating, power generation and heat transfer

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Frontiers in Energy 2017年第4期11卷 411-413页

作者： ya-Ling HE Ruzhu WANg Anthony Paul ROSKILLY Peiwen LI School of Energy and Power Engineering Xi'an Jiaotong University Xi'an 710049 China School of Mechanical Engineering Shanghai Jiao Tong University Shanghai 200240 China Sir Joseph Swan Centre for Energy Research Newcastle University Newcastle upon Tyne NE1 7RU UK Department of Aerospace and Mechanical Engineering The University of ArizonaTucson AZ 85721 USA

As a research focus, energy conservation has attracted a great deal of attention in recent years due to the energy crisis and environmental pollution concerns. Many countries made great efforts on both research and implementing energy conservation technologies. Cur- rently more than 50% of the total world＇s delivered energy is consumed by industry and about one sixth of the total energy consumed is wasted as low-grade heat, for example through radiation loss, exhaust gas flows, and cooling fluid circuits. Therefore, the recovery and reuse of waste heat is an effective way to significantly improve energy utilization. In addition, solar energy can provide low grade heat and is a clean and renewable form of energy. The efficient use of low grade heat from these sources can play an important role for a large number of applications.

关键词： Efficient use solar energy

Cardiometabolic and panvascular multimorbidity associated with motoric cognitive risk syndrome in older adults

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Journal of geriatric Cardiology 2024年第10期21卷 944-953页

作者： Rui SHE Zhong-Rui yaN Peng WANg ya-Jun LIANg Cheng-Xuan QIU Department of Rehabilitation Sciences the Hong Kong Polytechnic UniversityHong KongChina Department of ne-urology Jining No.1 People’s HospitalJiningChina Department of global Public Health Karolinska InstitutetStock-holmSweden Aging Research Center and Center for Alzheimer Research Department of NeurobiologyCare Sciences and SocietyKarolinska Institutet-Stockholm UniversityStockholmSweden Department of neurology Shandong Pr-ovincial Hospital Affiliated to Shandong First Medical UniversityJinanChina

Background Motoric cognitive risk(MCR)syndrome as a pre-dementia syndrome often co-occurring with chronic health *** study aims to investigate the prevalence of MCR and its association with cardiometabolic and panvascular multimorb-idity among older people living in rural *** This population-based study included 1450 participants who were aged≥60 years(66.2%women)and who underto-ok the second wave examination of the Confucius Hometown Aging Project in Shandong,China when information to define MCR was *** were collected through in-person interviews,clinical examinations,and laboratory *** and pa-nvascular multimorbidity were defined following the international *** was defined as subjective cognitive complaints and slow gait speed in individuals free of dementia and functional *** logistic regression models were used to exa-mine the associations of MCR with *** MCR was present in 6.3%of all participants,and the prevalence increased with advancing *** disea-se,ischemic heart disease,heart failure,and increased serum cystatin C were associated with increased likelihoods of MCR(mult-ivariable-adjusted odds ratio range:1.90-3.02,Pgher likelihood of *** fin-dings may have implications for identifying older adults at pre-dementia state as targets for early preventive interventions to delay dementia onset.

关键词： morbidity likelihood older

Multimodal treatment for spinal cord injury: a sword of neuroregeneration upon neuromodulation

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neural Regeneration Research 2020年第8期15卷 1437-1450页

作者： ya Zheng Ye-Ran Mao Ti-Fei Yuan Dong-Sheng Xu Li-Ming Cheng Rehabilitation Section Spine Surgery Division of Department of OrthopedicsTongji Hospital Affiliated to Tongji University School of MedicineShanghaiChina Shanghai Key Laboratory of Psychotic Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Co-innovation Center of neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education of the People’s Republic of China Tongji UniversityShanghaiChina Spine Surgery Division of Department of Orthopedics Tongji Hospital Affiliated to Tongji University School of MedicineShanghaiChina

Spinal cord injury is linked to the interruption of neural pathways,which results in irreversible neural *** repair and neuroregeneration are critical goals and issues for rehabilitation in spinal cord injury,which require neural stem cell repair and multimodal neuromodulation techniques involving personalized rehabilitation *** the involvement of endogenous stem cells in neurogenesis and neural repair,exogenous neural stem cell transplantation is an emerging effective method for repairing and replacing damaged tissues in central nervous system ***,to ensure that endogenous or exogenous neural stem cells truly participate in neural repair following spinal cord injury,appropriate interventional measures(e.g.,neuromodulation)should be *** techniques,such as noninvasive magnetic stimulation and electrical stimulation,have been safely applied in many neuropsychiatric *** is increasing evidence to suggest that neuromagnetic/electrical modulation promotes neuroregeneration and neural repair by affecting signaling in the nervous system;namely,by exciting,inhibiting,or regulating neuronal and neural network activities to improve motor function and motor learning following spinal cord *** studies have indicated that fine motor skill rehabilitation training makes use of residual nerve fibers for collateral growth,encourages the formation of new synaptic connections to promote neural plasticity,and improves motor function recovery in patients with spinal cord *** the development of biomaterial technology and biomechanical engineering,several emerging treatments have been developed,such as robots,brain-computer interfaces,and *** treatments have the potential to help millions of patients suffering from motor dysfunction caused by spinal cord ***,large-scale clinical trials need to be conducted to validate their *** review evaluated the efficacy of ne

关键词： brain-computer interface technology multimodal rehabilitation nerve regeneration neural circuit reconstruction neural regeneration neuromodulation rehabilitation training spinal cord injury stem cells transcranial direct current stimulation transcranial magnetic stimulation

WTO补贴规则与中国产业政策——中国集成电路增值税案引起的思考

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国际经济法学刊 2004年第3期 237-260页

作者：秦娅美国韦恩州立大学法学院

本文以中国在WTO被诉第一案,即中国集成电路增值税案为例,着重分析了WTO关于国内补贴的规则以及《中国加入世界贸易组织议定书》中某些特殊条款对我国产业政策潜在的制约和影响。文章认为补贴将是今后我国与其他WTO成员发生贸易纠纷的... 详细信息

本文以中国在WTO被诉第一案,即中国集成电路增值税案为例,着重分析了WTO关于国内补贴的规则以及《中国加入世界贸易组织议定书》中某些特殊条款对我国产业政策潜在的制约和影响。文章认为补贴将是今后我国与其他WTO成员发生贸易纠纷的一个重要原因,指出所谓中国的'非市场经济'地位可使其免受反补贴措施制约的说法的片面性,并就中国应采取的对策提出建议。

关键词：产业政策中国制约规则增值税补贴

Derivative Estimates for the Solution of Hyperbolic Affine Sphere Equation

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Communications in Mathematical Research 2015年第1期31卷 62-70页

作者： WU ya-DONg Rong Xiao-chun College of Mathematics and Information Science Jiangxi Normal University

Considering the hyperbolic affine sphere equation in a smooth strictly convex bounded domain with zero boundary values, the sharp derivative estimates of any order for its convex solution are obtained.

关键词： hyperbolic affine sphere Monge-Ampere equation derivative estimate