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Microfabricated polymer-metal biosensors for multifarious data collection from electrogenic cellular models

Microsystems & Nanoengineering 2023年第2期9卷 167-180页

作者： Charles M.Didier julia f.Orrico Omar S.Cepeda Trres Jorge Manrique Castro Aliyah Baksh Swaminathan Rajaraman NanoScience Technology Center University of Central Florida4353 Scorpius StreetResearch ISuite 231FL 32816 OrlandoUSA Burnett School of Biomedical Sciences University of Central Florida6900 Lake Nona BlvdFL 32827 OrlandoUSA Department of Biomedical Engineering Polytechnic University of Puerto Rico37700918Ponce de LeonSan JuanPuerto Rico Department of Electrical and Computer Engineering University of Central Florida4238 Scorpius StreetFL 32816 OrlandoUSA Department of Materials Science and Engineering University of Central Florida12760 Pegasus DriveEngineering ISuite 207FL 32816 OrlandoUSA

Benchtop tissue cultures have become increasingly complex in recent years,as more on-a-chip biological technologies,such as microphysiological systems(MPS),are developed to incorporate cellular constructs that more accurately represent their respective biological *** MPs have begun facilitating major breakthroughs in biological research and are poised to shape the field in the coming *** biological systems require integrated sensing modalities to procure complex,multiplexed datasets with unprecedented combinatorial biological *** this work,we expanded upon our polymer-metal biosensor approach by demonstrating a facile technology for compound biosensing that was characterized through custom modeling *** reported herein,we developed a compound chip with 3D microelectrodes,3D microfluidics,interdigitated electrodes(IDEs)and a *** chip was subsequently tested using the electrical/electrochemical characterization of 3D microelectrodes with 1 kHz impedance and phase recordings and IDE-based high-frequency(-1 MHz frequencies)impedimetric analysis of differential localized temperature recordings,both of which were modeled through equivalent electrical circuits for process parameter ***,a simplified antibody-conjugation strategy Was employed for a similar IDE-based analysis of the implications of a key analyte(L-glutamine)binding to the equivalent electrical ***,acute microfluidic perfusion modeling was performed to demonstrate the ease of microfluidics integration into such a polymer-metal biosensor platform for potential complimentary localized chemical ***,our work demonstrates the design,development,and characterization of an accessibly designed polymer-metal compound biosensor for electrogenic cellular constructs to facilitate comprehensive MPS data collection.

关键词： polymer recording breakthrough

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Nicotinamide mononucleotide:a potential effective natural compound against insulin resistance

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Signal Transduction and Targeted Therapy 2021年第9期6卷 2609-2610页

作者： julian Roos julia Zinngrebe Pamela fischer-Posovszky Department of Pediatrics and Adolescent Medicine Ulm University Medical CenterUlmGermany

Recently,a study published in Science by Yoshino et al.1 reported on a randomized,placebo-controlled,double-blind clinical trial to examine the effects of a 10-week nicotinamide mononucleotide(NMN)administration on human metabolism in 25 postmenopausal overweight or obese women with *** revealed positive effects of NMN on insulin sensitivity,insulin signalling,and tissue remodelling in skeletal muscle.

关键词： metabolism clinical

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AN IMPLEMENTATION Of THE COLLOCATION METHOD fOR INITIAL VALUE PROBLEMS

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International Journal of Modeling, Simulation, and Scientific Computing 2013年第2期4卷 142-154页

作者： RAfAEL G.campos fRANCISCO DOM´INGUEZ MOTA facultad de Ciencias fısico-Matematicas Universidad Michoacana 58060 MoreliaMich.Mexico

An implementation of the standard collocation method based on polynomial interpolation is presented in a matrix framework in this *** underlying differentiation matrix can be partitioned to yield a superconvergent implicit multistep-like method to solve the initial value problem *** first-and second-order versions of this method are L-stable.

关键词： IVP ODE collocation differentiation matrix interpolation superconvergence stability

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Sox9 in the developing central nervous system:a jack of all trades?

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Neural Regeneration Research 2021年第4期16卷 676-677页

作者： julia K.Vogel Michael Wegner Institut für Biochemie Emil-Fischer-ZentrumFriedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany

Sox9–gliogenesis and beyond:Neurons and glial cells are the major neuroectodermal cell types of the vertebrate central nervous system(CNS).Their generation from common progenitor cells takes place mostly during embryonic and early postnatal *** closure of the neural tube,neural epithelial progenitor cells(NEPs)establish the ventricular zone(VZ).By asymmetrical cell division,NEPs first give rise to neuronal precursor cells(NPs)that then differentiate into various types of ***,NEPs predominantly produce glial precursor cells that become either astroglia or oligodendroglia.

关键词： development. mostly closure

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Endothelial progenitor cells as a therapeutic option in intracerebral hemorrhage

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Neural Regeneration Research 2017年第4期12卷 558-561页

作者： Juan Pías-Peleteiro francisco campos José Castillo Tomás Sobrino Clinical Neurosciences Research Laboratory Department of NeurologyStroke UnitUniversity Clinical HospitalUniversidade de Santiago de CompostelaHealth Research Institute of Santiago de Compostela (IDIS)

Intracerebral hemorrhage （ICH） is the most severe cerebrovascular disease, which represents a leading cause of death and disability in developed countries. However, therapeutic options are limited, so is mandatory to investigate repairing processes after stroke in order to develop new therapeutic strategies able to promote brain repair processes. Therapeutic angiogenesis and vasculogenesis hold promise to improve outcome of ICH patients. In this regard, circulating endothelial progenitor cells （EPCs） have recently been suggested to be a marker of vascular risk and endothelial function. Moreover, EPC levels have been associated with good neurological and functional outcome as well as reduced residual hematoma volume in ICH patients. finally, experimental and clinical studies indicate that EPC might mediate endothelial cell regeneration and neovascularization. Therefore, EPC-based therapy could be an excellent therapeutic option in ICH. In this mini-review, we discuss the present status of knowledge about the possible therapeutic role of EPCs in ICH, molecular mechanisms, and the future perspectives and strategies for their use in clinical practice.

关键词： cellular therapy endothelial progenitor cells growth factors intracerebral hemorrhage neurorepair outcome

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Immunohistochemical Evaluation of Vascular Endothelial Growth factor(VEGf)in Splenic Hemangiomas and Hemangiosarcomas in Dogs

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Open Journal of Veterinary Medicine 2012年第4期2卷 191-195页

作者： Andressa Gianotti campos juliana Alvares Duarte Bonini campos Daniel Soares Sanches Maria Lucia Zaidan Dagli julia Maria Matera Department of Surgery School of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao PauloBrazil Department of Biostatistics and Scientific Method School of DentistrySao Paulo State UniversityAraraquaraBrazil Department of Animal Pathology School of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao PauloBrazil

formation of new blood vessels is paramount for tumour growth and metastatic dissemination and vascular endothelial growth factor (VEGf) is one of the key regulators of this process. The purpose of this study was to evaluate the immunohistochemical expression of VEGf in 23 splenic hemangiosarcomas and 7 splenic hemangiomas in dogs. Blood tests performed previous to splenectomy were analysed for correlation with tumour VEGf expression. Results showed significantly higher VEGf expression in hemangiosarcomas than hemangiomas and lower hematocrit values and red cell count in dogs affected with malignant neoplasia (P findings suggest the presence of high VEGf levels may be related to the malignant vascular proliferation seen in hemangiosarcomas.

关键词： Canine Spleen Angiogenesis Hemangiosarcoma Immunohistochemistry Vascular Endothelial Growth factor

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Influence of LDH conversion coatings on the adhesion and corrosion protection of friction spot-joined AA2024-T3/Cf-PPS

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Journal of Materials Science & Technology 2021年第8期67卷 197-210页

作者： A.C.Bouali N.M.Andre M.R.Silva campos M.Serdechnova J.f.dos Santos S.T.Amancio-filho M.L.Zheludkevich Institute of Materials Research Helmholtz-Zentrum G eesthacht.Magnesium Innovation CentreCorrosion and Surface TechnologyMax-Planck-Straβe 121502 CeesthachtGermany Institute of Materials Research Helmholtz-Zentrum GeesthachtMaterials MechanicsSolid State Joining ProcessesMax-Planck-Straβe 121502 CeesthachtGermany Graz University of Technology-TU Graz Institute of Materials Sciencejoining and FormingBMK Endowed Professorship for AviationKopemikusgasse 24/18010 GrazAustria faculty of Engineering University of KielKaiserstrasse 224143 KielGermany

Layered double hydroxide(LDH)conversion coatings loaded with corrosion inhibitors were suggested for the surface treatment of the aluminum alloy 2024-T3,prior to friction spot joining with carbon-fiber reinforced polyphenylene sulfide(AA2024-T3/Cf-PPS).Vanadate was used as a model corrosion *** shear testing method revealed an increase of approx.20%of the joint’s adhesion performance when treated with LDH and before exposure to salt *** evaluation of the joints after exposure to salt spray demonstrated a significant difference in the corrosion behavior of the joints when the AA2024-T3 is treated with LDH loaded with nitrate and vanadate *** LDH intercalated with nitrate revealed a clear improvement in the mechanical and corrosion resistance performance of the joints,even after 6 weeks of salt ***,the LDH intercalated with vanadate failed in providing protection against corrosion as well as preserving the mechanical properties of the *** effect of the galvanic corrosion was further investigated by zero resistance ammeter(ZRA)measurements as well as localized scanning vibrating electrode technique(SVET).

关键词： friction spot joining Layered double hydroxide Galvanic couple Corrosion protection Adhesion

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Environmental and biological factors affecting the abundance of Prosopis flexuosa saplings in the central-west Monte of Argentina

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forest Ecosystems 2022年第1期9卷 103-110页

作者： Cappa f.M. campos V.E. Barri f.R. Ramos L. campos C.M. INTERBIODES(Interacciones Biologicas del Desierto) Facultad Ciencias ExactasFísicas y Naturales(Universidad Nacional de San Juan)San JuanArgentina CIGEOBIO-CONICET(Centro de Investigaciones de la Geosfera y Biosfera Consejo Nacional de Investigaciones Científicas y Tecnicas)San JuanArgentina Instituto de Diversidad y Ecología Animal CONICET–UNCCordobaArgentina IADIZA Instituto Argentino de Investigaciones de las Zonas AridasUniversidad Nacional de CuyoGobierno de MendozaCONICETMendozaArgentina

Background:Trees and forests in drylands help mitigate the challenges through provision of economic products and vital environmental services such as habitat for biodiversity,prevention of erosion and desertification,regulation of water,microclimate,and soil *** condition and changes in dry forests can be assessed by using ecological indicators able to quantify spatial and temporal changes in *** of the ways to determine the condition of the forest is to study the dominant tree species and its *** study aimed to assess whether the abundance of Prosopis flexuosa saplings is affected by environmental and biological ***:To evaluate the first variables we used data from remote sensing such as satellite images and Aster Global Digital Model(GDEM).The second set of variables was about exotic and native ungulates and we used feces of these animals and camera traps to take *** found that sapling abundance related positively to sandy substrates and negatively to Wetness *** the other hand,in relation to biological variables,the abundance of saplings was positively affected by density of adult trees and by number of seeds dispersed by equines,but space use by Lama guanicoe had a negative relationship with *** research shows that *** saplings are benefited from sandy substrates and the conditions around adult *** addition to this,we found that exotic ungulates in low densities have neutral(***)or positive(***)effects on sapling ***:Based on these findings,we conclude that regeneration of the population of *** in our study area has no major *** addition,we corroborated that the presence of exotic and domestic ungulates in low densities does not have deleterious consequences for saplings of the dominant tree,***.

关键词： Bos primigenius taurus Dry forests Equines Exotic and domestic ungulates Lama guanicoe Prosopis flexuosa Remote sensing Saplings Seed dispersal

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Repression of the Arabidopsis thaliana Jasmonic Acid/Ethylene-Induced Defense Pathway by TGA-Interacting Glutaredoxins Depends on Their C-Terminal ALWL Motif

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Molecular Plant 2012年第4期5卷 831-840页

作者： Mark Zander Shuxia Chen julia Imkampe Corinna Thurow Christiane Gatz Albrecht-von-Hailer-Institute for Plant Sciences Georg-August-University Gottingen Julia-Lermontowa-Weg 3 D-37077 Gottingen Germany College of Horticulture Northwest A&F University Taicheng Road 3 Yangling Shaanxi 712100 PR China

Glutaredoxins are small heat-stable oxidoreductases that transfer electrons from glutathione （GSH） to oxi- dized cysteine residues, thereby contributing to protein integrity and regulation. In Arabidopsis thaliana, floral glutare- doxins ROXY1 and ROXY2 and pathogen-induced ROXY19/GRX480 interact with bZIP transcription factors of the TGACG （TGA） motif-binding family. ROXY1, ROXY2, and TGA factors PERIANTHIA, TGA9, and TGA10 play essential roles in floral development. In contrast, ectopically expressed ROXY19/GRX480 negatively regulates expression of jasmonic acid （JA）/ ethylene （ET）-induced defense genes through an unknown mechanism that requires clade II transcription factors TGA2, TGA5, and/or TGA6. Here, we report that at least 17 of the 21 land plant-specific glutaredoxins encoded in the Arabidopsis genome interact with TGA2 in a yeast-two-hybrid system. To investigate their capacity to interfere with the expression of JA/ET-induced genes, we developed a transient expression system. Activation of the ORA59 （OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERf-domain protein 59） promoter by transcription factor EIN3 （ETHYLENE INSENSITVE 3） was sup- pressed by co-expressed ROXY19/GRX480. Suppression depended on the L＊＊LL motif in the C-terminus of ROXY19/ GRX480. This putative protein interaction domain was recently described as being essential for the TGA/ROXY interaction. Ten of the 17 tested ROXY proteins suppressed ORA59 promoter activity, which correlated with the presence of the C-terminal ALWL motif, which is essential for ROXY1 function in flower development. ROXY19/GRX480-mediated repres- sion depended on the GSH binding site, suggesting that redox modification of either TGA factors or as yet unknown target proteins is important for the suppression of ORA59 promoter activity.

关键词： Arabidopsis thaliana cross-talk defense ethylene jasmonic acid glutaredoxins salicylic acid.

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Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice

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Cellular & Molecular Immunology 2018年第10期15卷 875-887页

作者： David Twapokera Mzinza Henrike fleige Kristin Laarmann Stefanie Willenzon Jasmin Ristenpart julia Spanier Gerd Sutter Ulrich Kalinke Peter Valentin-Weigand Reinhold förster Institute of Immunology Hannover Medical SchoolHannoverGermany Institute for Microbiology University of Veterinary Medicine HannoverHannoverGermany Institute for Experimental Infection Research TWINCORECentre for Experimental and Clinical Infection Researcha joint venture between the Helmholtz-Centre for Infection Research and the Hannover Medical SchoolHannoverGermany Institute for Infectious Diseases and Zoonoses University of Munich LMUMunichGermany

Bronchus-associated lymphoid tissue(BALT)develops at unpredictable locations around lung bronchi following pulmonary *** formation and composition of BALT have primarily been investigated by immunohistology that,due to the size of the invested organ,is usually restricted to a limited number of histological *** assess the entire BALT of the lung,other approaches are urgently ***,we introduce a novel light sheet microscopy-based approach for assessing lymphoid tissue in the *** antibody staining of whole lung lobes and optical clearing by organic solvents,we present a method that allows in-depth visualization of the entire bronchial tree,the lymphatic vasculature and the immune cell composition of the induced ***,three-dimensional analysis of the entire lung allows the qualitative and quantitative enumeration of the induced *** this approach,we show that a single intranasal application of the replication-deficient poxvirus MVA induces BALT that constitutes up to 8%of the entire lung volume in mice deficient in CCR7,in contrast to wild type mice(WT).furthermore,BALT induced by heat-inactivated *** is dominated by a pronounced T cell infiltration in Cxcr5-deficient mice,in contrast to WT mice.

关键词： Lung BALT light sheet microscopy E.coli MVA

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