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A Simple One-Step System Enhances the Availability of High-Quality Sperm for Assisted Reproductive Procedures

Open Journal of Obstetrics and Gynecology 2023年第10期13卷 1676-1687页

作者： Sam Prien Trinity Moseley Nirvika Singh-Sharma juergen liebermann Matthew D. VerMilyea Lindsay L. Penrose Department Ob/Gyn Texas Tech University Health Sciences Center Lubbock USA Department Animal and Food Sciences Texas Tech University Lubbock USA Fertility Center of Illinois Chicago USA Ovation Fertility San Antonio USA

Over the last forty years, in vitro fertilization, which has expanded to assisted reproductive technologies (ART), has gone from an experimental procedure to the mainstay of infertility treatment. A technique that once made news with each birth is now responsible for 2% - 3% of the babies born in several nations of the world. This has happened due to significant advances in hormone therapies, culture techniques, and the specialization of equipment designed to support oocytes and embryos. However, for all the advances made to support female fertility, little has changed in male treatment since the advent of intracytoplasmic sperm injection in the early 1990’s. Recently, a number of authors have documented problems with sperm preparation techniques. Some report DNA damage, others membrane and organelle issues, all of which potentially hamper fertilization rates and possibly take-home baby rates. Further, as the clinical workload of ART has increased and staffing shortages have become critical, all labs are looking for simpler, more efficient ways to perform job functions. This study describes a simple, one-step method for preparing semen samples for ART. This new technique minimizes excessive manipulation of the sample compared to current standards and is less likely to cause cell damage. Preliminary results suggest a significant enhancement in recovered sample motility and an optimal sample for ART procedures with minimal sample manipulation.

关键词： Simple Enhanced Sperm Processing

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