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Plasma membrane calcium ATPase 4b inhibits nitric oxide generation through calcium-induced dynamic interaction with neuronal nitric oxide synthase

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Protein & Cell 2013年第4期4卷 286-298页

作者： Wenjuan Duan juefei zhou Wei Li Teng zhou Qianqian Chen Fuyu Yang Taotao Wei National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Research Department of Bacterial Vaccine Chengdu Institute of Biological Products Co.LtdChengdu 610023China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China

The activation and deactivation of Ca^(2+)- and calmodulindependent neuronal nitric oxide synthase (nNOS) in the central nervous system must be tightly controlled to prevent excessive nitric oxide (NO) generation. Considering plasma membrane calcium ATPase (PMCA) is a key deactivator of nNOS, the present investigation aims to determine the key events involved in nNOS deactivation of by PMCA in living cells to maintain its cellular context. Using time-resolved Förster resonance energy transfer (FRET), we determined the occurrence of Ca^(2+)-induced protein-protein interactions between plasma membrane calcium ATPase 4b (PMCA4b) and nNOS in living cells. PMCA activation significantly decreased the intracellular Ca 2+ concentrations ([Ca^(2+)]_(i)), which deactivates nNOS and slowdowns NO synthesis. Under the basal [Ca^(2+)]_(i) caused by PMCA activation, no protein-protein interactions were observed between PMCA4b and nNOS. Furthermore, both the PDZ domain of nNOS and the PDZ-binding motif of PMCA4b were essential for the protein-protein interaction. The involvement of lipid raft microdomains on the activity of PMCA4b and nNOS was also investigated. Unlike other PMCA isoforms, PMCA4 was relatively more concentrated in the raft fractions. Disruption of lipid rafts altered the intracellular localization of PMCA4b and affected the interaction between PMCA4b and nNOS, which suggest that the unique lipid raft distribution of PMCA4 may be responsible for its regulation of nNOS activity. In summary, lipid rafts may act as platforms for the PMCA4b regulation of nNOS activity and the transient tethering of nNOS to PMCA4b is responsible for rapid nNOS deactivation.

关键词： plasma membrane calcium ATPase neu-ronal nitric oxide synthase calcium nitric oxide lipid raft Förster resonance energy transfer

An aircraft brake control algorithm with torque compensation based on RBF neural network

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Chinese Journal of Aeronautics 2024年第1期37卷 438-450页

作者： Ning BAI Xiaochao LIU juefei LI Zhuangzhuang WANG Pengyuan QI Yaoxing SHANG Zongxia JIAO School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China Science and Technology on Aircraft Control Laboratory Beihang UniversityBeijing 100191China Key Laboratory of Advanced Aircraft Systems(Beihang University) Ministry of Industry and Information TechnologyBeijing 100091China Research Institute for Frontier Science Beihang UniversityBeijing 100191China Ningbo Institute of Technology of Beihang University Ningbo 315800China Tianmushan Laboratory Xixi Octagon CityYuhang DistrictHangzhou 310023China

The wheel brake system of an aircraft is the key to ensure its safe landing and rejected takeoff.A wheel’s slip state is determined by the brake torque and ground adhesion torque,both of which have a large degree of *** is this nature that brings upon the challenge of obtaining high deceleration rate for aircraft brake *** overcome the disturbances caused by the above uncertainties,a braking control law is designed,which consists of two parts:runway surface recognition and wheel’s slip state *** runway surface recognition,the identification rules balancing safety and braking efficiency are defined,and the actual identification process is realized through recursive least square method with forgetting *** slip state tracking,the LuGre model with parameter adaptation and a brake torque compensation method based on RBF neural network are proposed,and their convergence are *** effectiveness of our control law is verified through simulation and ground *** in the experiments on the ground inertial test bench,compared to the improved pressure-biased-modulation(PBM)anti-skid algorithm,fewer wheel slips occur,and the average deceleration rate is increased by 5.78%,which makes it a control strategy with potential for engineering applications.

关键词： Aircraft landing Brakes Disturbance observer Neural networks Runway identification

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京津冀一体化中智慧城市发展水平实证分析

智能城市 2020年第13期6卷 1-3页

作者：高娃 juefei zhou 北京理工大学数学与统计学院北京100081 Ontario Tech University CanadaOshawaOntario L1G OC5

为实证分析京津冀一体化中智慧城市发展水平,构建了包括经济水平、环境治理、基础设施、科技创新和信息产业等5个一级指标和16个二级指标的评价指标体系,应用主成分法对北京、天津及河北的石家庄、张家口、秦皇岛和沧州6个城市的2018年... 详细信息

为实证分析京津冀一体化中智慧城市发展水平,构建了包括经济水平、环境治理、基础设施、科技创新和信息产业等5个一级指标和16个二级指标的评价指标体系,应用主成分法对北京、天津及河北的石家庄、张家口、秦皇岛和沧州6个城市的2018年统计年鉴中的相关数据进行实证分析,得出智慧城市发展水平的综合性因子、经济因子和居民生活质量因子共3个主成分因子及综合评价模型。根据各城市综合评价得分及排名来评价各城市智慧发展水平,据此提出促进京津冀一体化中智慧城市发展的政策建议。

关键词：智慧城市京津冀一体化主成分分析

《全球化学品统一分类和标签制度》中加和法不适用时的混合物分类

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肥料与健康 2023年第3期50卷 69-73,76页

作者：周珏斐上海化工院检测有限公司上海200062

《全球化学品统一分类和标签制度》(GHS)是由联合国出版的指导各国建立统一化学品分类和标签制度的规范性文件。简要介绍了GHS中化学品分类的方法,针对混合物分类的复杂性,通过2个案列详细分析了加和法不适用时的混合物分类的方法。同... 详细信息

《全球化学品统一分类和标签制度》(GHS)是由联合国出版的指导各国建立统一化学品分类和标签制度的规范性文件。简要介绍了GHS中化学品分类的方法,针对混合物分类的复杂性,通过2个案列详细分析了加和法不适用时的混合物分类的方法。同时提出在进行危险性分类时若采用的方法不当或引用的数据资料不同,会产生截然不同的分类结果,影响化学品内在危险性的判断和辨识。

关键词： GHS 加和法混合物危险性分类

数字乡村背景下农村电子商务数字化转型路径探索

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数字通信世界 2024年第5期 149-151页

作者：梁珏菲陇南师范高等专科学校甘肃陇南742500

该文旨在探索数字乡村背景下农村电子商务数字化转型的路径,概述了数字乡村和农村电子商务的特点和发展现状,阐述了农村电子商务数字化转型的必要性及关键要素,具体分析了传统农村电商存在的问题,并提出相应的数字化转型策略。

关键词：数字乡村农村电子商务数字化转型

Down Regulation of nNOS Activity by PMCA4 via PDZ Domain-dependent Protein-protein Interactions

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Down Regulation of nNOS Activity by PMCA4 via PDZ Domain-dep...

The regulation of nNOS activity by PMCA and lipid rafts

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Wenjuan Duan1,juefei zhou 1,Wei Li 2,Taotao Wei 1,Fuyu Yang 1 1 Institute of Biophysics,Chinese Academy of Sciences,15 Datun Road,Chaoyang District,Beijing,100101,China, 2 Institute of High Energy Physics,Chinese Academy of Sciences,19B Yuquan Road, Shijingshan District,Beijing,10049,China

＜正＞The main protease（Mpro）of SARS-CoV plays an essential role in the extensive proteolytic processing of the viral polyproteins（pp1a and pp1ab）,and it is an important target for

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The regulation of nNOS activity by PMCA and lipid rafts

Down Regulation of nNOS Activity by PMCA4 via PDZ Domain-dependent Protein-protein Interactions

The 2nd International and 11th National Symposium on Membrane Biology

作者： Wenjuan Duan~1 juefei zhou~(1 2) Wei Li~3 Taotao Wei~1 Fuyu Yang~1 1.Institute of Biophysics Chinese 15 Datun Road Chaoyang District Beijing 100101 China 2.Reasearch Department of Bacterial Vaccine Chengdu Institute of Biological Products Chengdu China 3.Institute of High Energy Physics Chinese 19B Yuquan Road Shijingshan District Beijing 100049 China

＜正＞Nitric oxide（NO） is an important signaling molecule in the central nervous *** is generated by the neuronal nitric oxide synthase（nNOS） under the regulation of multiple *** studies showed that plasma membrane calcium ATPase（PMCA） might be a key regulator of *** the PMCA is a lipid raft-related membrane protein,the role of lipid raft microdomains of the plasma membrane in this regulation process remains to be *** we showed that when activated,PMCA4b could interact with nNOS and consequently down regulated the activity of nNOS,and this process was depending on the integrity of the lipid raft microdomains.

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生物物理学报 2009年第S1期25卷 199-199页

作者： Wenjuan Duan1, juefei zhou1, Wei Li2, Taotao Wei1, Fuyu Yang1 1 Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101, China, 2 Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Road, Shijingshan District, Beijing, 10049, China

The main protease (Mpro) of SARS-CoV plays an essential role in the extensive proteolytic processing of the viral polyproteins (pp1a and pp1ab), and it is an important target

关键词： Activity nNOS

High-energy-density pentazolate salts:CaN_(10)and BaN_(10)

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Science China(Physics,Mechanics & Astronomy) 2021年第1期64卷 102-107页

作者： JiaNan Yuan Kang Xia juefei Wu Jian Sun National Laboratory of Solid State Microstructures School of Physics and Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing210093China

The search for high energy density materials(HEDMs)in polymeric nitrogen compounds has gained considerable *** theoretical predictions and experiments have revealed that metal ions can be used to stabilize the pentazolate(N-5)*** this work,by employing a machine learning-accelerated crystal structure searching method and first-principles calculations,we found that the new pentazolate salts,CaN(10)and BaN(10),are energetically favorable at high *** dispersion calculations reveal that they are quenchable at ambient *** initio molecular dynamics simulations verify their dynamic stability at finite *** charge and electron localization function illustrates that alkaline earth atoms serve as electron donors,contributing to the stability of N5 *** calculations reveal covalent bonds between nitrogen atoms and weak interactions between N5 *** to other pentazolate salts,these polymeric nitrides have high energy densities of approximately 2.35 kJ/g for CaN(10)and 1.32 kJ/g for BaN(10).The predictions of CaN(10)and BaN(10)structures indicate that these salts are potential candidates for green nitrogen-rich HEDMs.

关键词： high energy density material polymeric nitrogen pentazolate salts