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Aggregate 2024年第1期5卷 244-256页

作者： Xu Chen Meijiao Zhao Qihu Xie Sitong Zhou Xiaoping Zhong judun zheng Ronghua Yang Xianjin Du Jinyu Xia Yuhui Liao Department of Infectious Diseases The Fifth Affiliated HospitalSun Yat-sen UniversityZhuhaiChina Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology HospitalSouthern Medical UniversityGuangzhouChina Department of Burns and Plastic Surgery The Second Affiliated Hospital of Shantou University Medical CollegeShantouChina Department of Dermatology TheFirst People’s Hospital of FoshanFoshanChina Department of Burn and Plastic Surgery Guangzhou First People’s HospitalSouth China University of TechnologyGuangzhouChina Department of Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhanChina

As a high-risk trauma,deep burns are always hindered in their repair process by decreased tissue regeneration capacity and persistent *** this study,we developed a simultaneous strategy for deep burn wounds treatment using functional nanovesicles with antibacterial and tissue remodeling properties,delivered via a click-chemistry *** aggregation-induced emission photosensitizer of 4-(2-(5-(4-(diphenylamino)phenyl)thiophen-2-yl)vinyl)-1-(2-hydroxyethyl)pyridin-1-ium bromide(THB)with excellent photodynamic properties was first prepared,and then combined with readily accessible adipose stem cells-derived nanovesicles to generate the THB functionalized nanovesicles(THB@ANVs).The THB@ANVs showed strong antibacterial activity against Gram-positive bacteria(up to 100%killing rate),and also beneficial effects on tissue remodeling,including promoting cell migration,cell proliferation,and regulating *** addition,we prepared a click-hydrogel of carboxymethyl chitosan for effective delivery of THB@ANVs on *** hydrogel could be injected to conform to the wound morphology while responding to the acidic *** vivo evaluations of wound healing revealed that the THB@ANVs hydrogel dressing efficiently accelerated the healing of second-degree burn wounds by reducing bacterial growth,regulating inflammation,promoting early angiogenesis,and collagen *** study provides a promising candidate of wound dressing with diverse functions for deep burn wound repair.

关键词： aggregation-induced emission burn wounds nanovesicles

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Synchronous detection of glutathione/hydrogen peroxide for monitoring redox status in vivo with a ratiometric upconverting nanoprobe

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Nano Research 2019年第4期12卷 931-938页

作者： judun zheng Yunxia Wu Da Xing Tao Zhang MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science South China Normal University Guangzhou 510631 China College of Biophotonics South China Normal University Guangzhou 510631 China

Cellular redox status presents broad implications with diverse physiological and pathological processes. Simultaneous detection of both the oxidative and reductive species of redox couples, especially the most representative pair glutathione/hydrogen peroxide (GSH/H2O2), is crucial to accurately map the cellular redox status. However, it still remains challenging to synchronously detect GSH/H2O2 in vivo due to lack of a reliable measuring tool. Herein, a ratiometric nanoprobe (UCNP-TB) possessing simultaneous delectability of GSH/H2O2 is established based on a multi-spectral upconverti ng nano phosphor (UCNP-OA) as the lumin esce nee res onance energy tran sfer (LRET) donor and two dye molecules as the acceptors, including a GSH-sensitive dye (TCG) and a H2O2-sensitive dye (BCH). With the as-prepared UCNP-TB, real-time and synchronous monitoring the variation of GSH and H2O2 in vitro and in living mice can be achieved using the ratio of the upcon versi on lumin esce nee (UCL) at 540 and 650 nm to that at 800 nm as the detecti on sign al, respectively, providi ng highly inhere nt reliability of the sensing results by self-calibrati on. Moreover, the nan oprobe is capable of mappi ng the redox status within the drug-resista nt tumor and the drug-induced hepatotoxic liver via ratiometric UCL imaging. Thus, this nan oprobe would provide a reliable tool to elucidate the redox state in vivo.

关键词： redox status glutathione hydrogen peroxide upconversion imaging nanoprobe

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Light-responsive charge-reversal nanovector for high-efficiency in vivo CRISPR/Cas9 gene editing with controllable location and time

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Nano Research 2020年第9期13卷 2399-2406页

作者： Yunxia Wu judun zheng Qin Zeng Tao Zhang Da Xing MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Controllably and efficaciously localized CRISPR/Cas9 plasmids transfection plays an essential role in genetic editing associated with various key human *** employed near-infrared(NIR)light-responsive CRISPR/Cas9 plasmids delivery via a charge-reversal nanovector to achieve highly efficient and site-specific gene *** nanovector with abundant positive charges was fabricated on the basis of an ultraviolet-sensitive conjugated polyelectrolyte coated on an upconversion nanomaterial(UCNP-UVP-P),which can convert into negative charges upon 980 nm light *** the as-prepared nanovector,we demonstrated the plasmids could be efficiency transfected into tumor cells(~63%±4%)in a time-contolled manner,and that functional CRISPR/Cas9 proteins could be successfully expressed in a selected NIR-irradiated ***,this strategy was successfully applied to the delivery of CRISPR/Cas9 gene to tumor cells in vivo,inducing high efficiency editing of the target gene PLK-1 under ***,this precisely controlled gene regulation strategy has the potential to serve as a new paradigm for gene engineering in complex biological systems.

关键词： light-responsive charge-reversal CRISPR/Cas9 gene editing

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梅毒患者住院费用及其影响因素的通径分析

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中国病案 2024年第8期25卷 32-35页

作者：林金雄索斯琴郑举敦申树群南方医科大学皮肤病医院(广东省皮肤病医院) 广东省广州市510091 南方医科大学卫生管理学院广东省广州市510515

目的研究梅毒住院患者次均住院费用现状并分析其影响因素,为合理有效率控制医院住院费用上涨、减轻患者就医负担提供科学性建议。方法收集广东省某三级皮肤病专科医院2015年1月1日-2019年12月31日538例梅毒住院患者病例资料,运用描述性... 详细信息

目的研究梅毒住院患者次均住院费用现状并分析其影响因素,为合理有效率控制医院住院费用上涨、减轻患者就医负担提供科学性建议。方法收集广东省某三级皮肤病专科医院2015年1月1日-2019年12月31日538例梅毒住院患者病例资料,运用描述性分析、单因素t检验、通径分析及中介效应检验等方法对梅毒住院患者住院费用基本状况进行描述,并探究各影响因素与住院费用间相关关系。结果梅毒患者平均住院费4931.33元,平均住院天数5.99天,通径分析显示住院天数(β=0.538,P<0.001)、是否使用抗菌药物(β=0.227,P=0.006)、是否医保(β=0.147,P<0.001)、是否手术(β=0.223,P<0.001)会直接影响总费用,其中性别(β=0.029,P=0.005)、是否使用抗菌药物(β=0.417,P<0.001)、是否手术(β=-0.036,P=0.003)通过影响住院天数间接影响住院费用。结论影响住院费用的因素主要是住院天数、是否使用抗菌药物、是否手术。

关键词：梅毒住院费用影响因素通径分析中介效应

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Synchronous detection of glutathione/hydrogen peroxide for monitoring redox status in vivo with a ratiometric upconverting nanoprobe

Synchronous detection of glutathione/hydrogen peroxide for m...

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第十一届全国化学生物学学术会议

作者： judun zheng Yunxia Wu Da Xing Tao Zhang MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science South China Normal University College of Biophotonics South China Normal University

Cellular redox status presents broad implications with diverse physiological and pathological *** detection of both the oxidative and reductive species of redox couples,especially the most representative pair glutathione/hydrogen peroxide(GSH/HO),is crucial to accurately map the cellular redox ***,it still remains challenging to synchronously detect GSH/HO in vivo due to lack of a reliable measuring ***,a ratiometric nanoprobe(UCNP-TB) possessing simultaneous delectability of GSH/HO is established based on a multi-spectral upconverting nanophosphor(UCNP-OA) as the luminescence resonance energy transfer(LRET) donor and two dye molecules as the acceptors,including a GSH-sensitive dye(TCG) and a HO-sensitive dye(BCH).With the as-prepared UCNP-TB,real-time and synchronous monitoring the variation of GSH and HO in vitro and in living mice can be achieved using the ratio of the upconversion luminescence(UCL) at 540 and 650 nm to that at 800 nm as the detection signal,respectively,providing highly inherent reliability of the sensing results by ***,the nanoprobe is capable of mapping the redox status within the drug-resistant tumor and the drug-induced hepatotoxic liver via ratiometric UCL ***,this nanoprobe would provide a reliable tool to elucidate the redox state in vivo.

关键词： redox status glutathione hydrogen peroxide upconversion imaging nanoprobe

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梅毒螺旋体及其相关蛋白与不同宿主细胞相互作用的研究进展

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中国皮肤性病学杂志 2023年第3期37卷 362-366页

作者：岳锐郑举敦廖玉辉杨斌南方医科大学皮肤病医院广东广州510091

梅毒是由梅毒螺旋体引起的性传播疾病,可造成全身多脏器损害。梅毒螺旋体致病机制涉及暴露、黏附/定植、侵袭/感染、播散等过程。在这些过程中梅毒螺旋体与宿主细胞存在长期的相互作用,病原体本身及其相关蛋白的致病性与宿主的免疫防御... 详细信息

梅毒是由梅毒螺旋体引起的性传播疾病,可造成全身多脏器损害。梅毒螺旋体致病机制涉及暴露、黏附/定植、侵袭/感染、播散等过程。在这些过程中梅毒螺旋体与宿主细胞存在长期的相互作用,病原体本身及其相关蛋白的致病性与宿主的免疫防御间的平衡在相互作用中扮演重要角色。本文就梅毒螺旋体及其相关蛋白与不同宿主细胞相互作用的研究进展综述如下。

关键词：梅毒螺旋体蛋白宿主细胞相互作用

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稀土卟啉近红外发光配合物在生命科学领域中的应用研究进展

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激光生物学报 2016年第3期25卷 193-197页

作者：张涛郑举敦吴云霞华南师范大学激光生命科学暨教育部重点实验室生物光子学研究院广东广州510631

稀土近红外发光材料具有独特的光物理性质,如发光谱带窄、较大的Stock位移、荧光寿命长可达毫秒级等,在医学诊断和成像、免疫分析等热门领域具有重大的应用前景。但由于跃迁选择定则,稀土离子本身的吸收系数较小,需要用特定的生色团对... 详细信息

稀土近红外发光材料具有独特的光物理性质,如发光谱带窄、较大的Stock位移、荧光寿命长可达毫秒级等,在医学诊断和成像、免疫分析等热门领域具有重大的应用前景。但由于跃迁选择定则,稀土离子本身的吸收系数较小,需要用特定的生色团对其进行敏化,以增强其发光性能。在众多生色团中,卟啉化合物由于其激发态能级与近红外发光的稀土离子能级较为匹配,可以较好的敏化稀土离子,获得较高的近红外发光效率,因此,近年来受到了极大的关注。本文总结了近年来近红外发光卟啉稀土配合物在生命科学领域中的应用研究进展,并对其发展前景进行了展望。

关键词：近红外发光稀土卟啉

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一种新型细胞摄取增强的两亲性卟啉类光敏剂及肿瘤光动力治疗研究

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激光生物学报 2016年第3期25卷 234-239页

作者：张涛郑举敦马腾马雪敏方文凯吴宝艳华南师范大学激光生命科学暨教育部重点实验室生物光子学研究院广东广州510631

光敏剂能否被肿瘤细胞高效吸收是影响光动力治疗效率的重要因素。亲脂性光敏剂易于被肿瘤组织摄取,但会使光敏剂发生自猝灭;亲水性光敏剂则有利于光敏剂在体内的转运,但肿瘤细胞摄取率会下降。本工作通过亲酯性的乙二醇缩合支链和亲水... 详细信息

光敏剂能否被肿瘤细胞高效吸收是影响光动力治疗效率的重要因素。亲脂性光敏剂易于被肿瘤组织摄取,但会使光敏剂发生自猝灭;亲水性光敏剂则有利于光敏剂在体内的转运,但肿瘤细胞摄取率会下降。本工作通过亲酯性的乙二醇缩合支链和亲水性季膦基团与卟啉相连接,成功制备一种新型两亲性卟啉锌化合物(ZnTP-TP),实验表明该化合物具有较高的单线态氧量子产率和良好的两亲性,可被细胞快速摄取,并表现出较低的细胞毒性和良好的肿瘤光动力治疗效应。

关键词：光动力治疗卟啉细胞摄取率

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