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Temporal and spatial control of gene expression inhorticultural crops

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Horticulture Research 2014年第1期1卷 69-85页

作者： Manjul Dutt Sadanand A Dhekney Leonardo Soriano Raju Kandel jude w grosser Citrus Research and Education Center University of Florida700 Experiment Station RoadLake AlfredFL 33850USA Department of Plant Sciences Sheridan Research and Extension CenterUniversity of WyomingSheridanWY 82801USA Universidade de Sao Paulo Centro de Energia Nuclear na AgriculturaPiracicabaBrazil

Biotechnology provides plant breeders an additional tool to improve various traits desired by growers and consumers of horticultural *** also provides genetic solutions to major problems affecting horticultural crops and can be a means for rapid improvement of a *** the availability of a number of horticultural genome sequences,it has become relatively easier to utilize these resources to identify DNA sequences for both basic and applied *** play a key role in plant gene expression and the regulation of gene *** recent years,rapid progress has been made on the isolation and evaluation of plant-derived promoters and their use in horticultural crops,as more and more species become amenable to genetic *** understanding of the tools and techniques of horticultural plant biotechnology has now evolved from a discovery phase to an implementation *** availability of a large number of promoters derived from horticultural plants opens up the field for utilization of native sequences and improving crops using precision *** this review,we look at the temporal and spatial control of gene expression in horticultural crops and the usage of a variety of promoters either isolated from horticultural crops or used in horticultural crop improvement.

关键词： expression. crops utilize

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES wITH/ORAH/MANDARIN(CITRUS RETICULATA)

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Frontiers of Agricultural Science and Engineering 2021年第2期8卷 302-313页

作者： Qiangming XIA wei wANG Kaidong XIE Xiaomeng wU Xiuxin DENG jude wgrosser wenwu GUO Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China Citrus Research and Education Center University of Florida/IFASLake AlfredUSA.

Seedless fruits are desirable in the citrus fresh fruit *** production via diploid x tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless ***'Orah'mandarin has desirable organoleptic qualities,seeds in the fruits weaken its market *** produce new seedless cultivars that are similar to'Orah'mandarin,we performed three 2x x 4x crosses using'Orah'mandarin as the seed parent to regenerate triploid plantlets.A total of 182 triploid and 36 tetraploid plantlets were *** analyzing their genetic origins using nine novel single nucleotide polymorphism(SNP)markers,all of the triploids and tetraploids derived from these three crosses were proven to be ***,we demonstrated that 2n megagametophyte formation in'Orah'mandarin result in tetraploid production in these three interploidy *** tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP *** on the genotypes of the 2n megagametophytes,the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%,indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual *** triploid hybrids enrich the germplasm available for seedless ***,the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

关键词： Citrus 2n gamete interploidy hybridization pericentromeric SNP marker second division restitution

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Rationale for reconsidering current regulations restricting use of hybrids in orange juice

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Horticulture Research 2020年第1期7卷 2375-2381页

作者： Ed Stover Frederick G.Gmitter Jr jude grosser Elizabeth Baldwin Guohong Albert wu Jinhe Bai Yu wang Peter Chaires Juan Carlos Motamayor USDA/ARS US Horticultural Research Lab.2001 S Rock Rd.Ft.PierceFL 34945USA Citrus Research and Education Center Univ.Florida700 Experiment Station Rd.Lake AlfredFL 33850USA US Department of Energy Joint Genome InstituteLawrence Berkeley National Laboratory1 Cyclotron RoadBerkeleyCA 94720USA New Varieties Development&Management Corp. PO Box 941058MaitlandFL 32794USA The Coca-Cola Company 2651 Orange Ave.ApopkaFL 32703USA

Huanglongbing(HLB)is a disease that has devastated the Florida citrus industry,threatens the entire *** industry,and globally is rapidly ***’s citrus production is 90%sweet orange,which is quite sensitive to *** heavy reliance on sweet orange for Florida citrus production makes the industry especially vulnerable to diseases that are damaging to this type of ***,90%of Florida oranges are used in producing orange juice that is defined by a federal regulation known as the“orange juice standard”,specifying that at least 90%of“orange juice”must be derived from Citrus *** analyses definitively reveal that sweet orange is not a true species,but just one of many introgression hybrids of *** and ***,with phenotypic diversity resulting from accumulated mutations in this single hybrid,the“sweet orange”.No other fruit industry is limited by law to such a narrow genetic ***,there are new citrus hybrids displaying reduced sensitivity to HLB,and in some cases they produce juice,alone or in blends,that consumers would recognize as“orange juice”.Reconsidering current regulations on orange juice standards may permit use of such hybrids in“orange juice”,providing greater latitude for commercialization of these hybrids,leading to higher-quality orange juice and a more sustainable Florida orange juice industry.

关键词： orange Rational stated

以本地早橘和槾橘为母本倍性杂交创制柑橘三倍体

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中国农业科学 2020年第23期53卷 4961-4968页

作者：解凯东彭珺袁东亚强瑞瑞谢善鹏周锐夏强明伍小萌柯甫志刘高平 grosser jude w 郭文武华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室浙江省农业科学院柑桔研究所浙江省台州市黄岩区果树技术推广总站美国佛罗里达大学柑橘研究与教育中心

【目的】基于二倍体与四倍体倍性杂交策略创制柑橘三倍体新种质。【方法】以二倍体为母本,四倍体为父本进行人工授粉,授粉后85 d采摘幼果并对未成熟种子实施幼胚离体挽救培养;再生植株后,用流式细胞仪和根尖染色体计数法对再生植株进行... 详细信息

【目的】基于二倍体与四倍体倍性杂交策略创制柑橘三倍体新种质。【方法】以二倍体为母本,四倍体为父本进行人工授粉,授粉后85 d采摘幼果并对未成熟种子实施幼胚离体挽救培养;再生植株后,用流式细胞仪和根尖染色体计数法对再生植株进行倍性鉴定;并用SSR标记对三倍体后代进行分子鉴定。【结果】以本地早橘和槾橘为母本,8个异源四倍体体细胞杂种和1个双二倍体为父本,配置9个倍性杂交组合,共授粉2 749朵花,坐果489个,平均坐果率17.8%;培养幼嫩种子2 239粒,经幼胚离体挽救培养,共再生植株260株;用流式细胞仪和根尖染色体计数检测再生植株倍性,获得三倍体141株;SSR分子鉴定表明从槾橘×NS组合随机选取的50株三倍体后代全部为双亲的有性后代;三倍体后代在温室生长一年后,采用嫁接(枳砧)将这些三倍体定植于田间。【结论】这些三倍体新种质为本地早橘和槾橘无核新品种培育奠定了宝贵的材料基础。

关键词：柑橘无核育种三倍体幼胚离体挽救培养流式细胞仪

单胚性二倍体为母本与异源四倍体杂交大规模创制柑橘三倍体

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中国农业科学 2013年第21期46卷 4550-4557页

作者：解凯东王惠芹王晓培梁武军谢宗周伊华林邓秀新 grosser jude w 郭文武华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室美国佛罗里达大学柑橘研究与教育中心

【目的】以单胚性的二倍体柑橘品种为母本与异源四倍体体细胞杂种杂交培育三倍体植株。【方法】选择亲本配置杂交组合进行人工授粉,授粉后70—100 d采幼果进行胚挽救,以流式细胞仪结合根尖染色体压片对再生植株进行倍性检测,最后进行SS... 详细信息

【目的】以单胚性的二倍体柑橘品种为母本与异源四倍体体细胞杂种杂交培育三倍体植株。【方法】选择亲本配置杂交组合进行人工授粉,授粉后70—100 d采幼果进行胚挽救,以流式细胞仪结合根尖染色体压片对再生植株进行倍性检测,最后进行SSR分子标记鉴定。【结果】2009—2012连续4年,以8个二倍体为母本、以4个异源四倍体体细胞杂种为父本,共配置14个倍性杂交组合,授粉花数3 347朵,坐果数678个,平均坐果率20.26%。实施胚挽救的果实数505个,培养种子12 357粒,经生芽、生根诱导培养共获得1 022个株系。通过对再生植株的倍性检测,共获得三倍体植株755个株系,四倍体19个株系。对华农红柚×NH组合的三倍体、四倍体后代进行分子鉴定,表明三倍体和四倍体全部为双亲的有性杂种后代。【结论】本研究通过杂交获得的三倍体为我国无籽柑橘品种选育奠定了材料基础;同时获得的四倍体后代也为未来的柑橘三倍体育种提供了优良的亲本材料。

关键词：柑橘三倍体有性杂交倍性分析 SSR标记

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以柑橘多胚性二倍体母本倍性杂交培育三倍体

园艺学报 2014年第4期41卷 613-620页

作者：解凯东王晓培王惠芹梁武军谢宗周郭大勇伊华林邓秀新 grosser jude w. 郭文武华中农业大学园艺植物生物学教育部重点实验室/农业部华中地区园艺作物生物学与种质创制重点实验室武汉430070 美国佛罗里达大学柑橘研究与教育中心

2009—2012年,以柑橘8个多胚性二倍体品种为母本与近年始花的5个异源四倍体体细胞杂种和1个同源四倍体分别杂交,培育三倍体新种质。连续4年共配置15个杂交组合,授粉4 442朵花,坐果1 484个,平均坐果率33.4%;用于幼胚挽救的果实1 075个,... 详细信息

2009—2012年,以柑橘8个多胚性二倍体品种为母本与近年始花的5个异源四倍体体细胞杂种和1个同源四倍体分别杂交,培育三倍体新种质。连续4年共配置15个杂交组合,授粉4 442朵花,坐果1 484个,平均坐果率33.4%;用于幼胚挽救的果实1 075个,共培养幼嫩种子12 578颗,经生芽、生根诱导获得再生植株2 832个;以流式细胞仪对所有再生植株倍性检测,15个组合共获得三倍体401个,四倍体121个;移栽成活三倍体349个,四倍体98个。对w.默科特×NH组合89个株系的三倍体的SSR分子鉴定表明,所有三倍体后代均为双亲的有性杂种。

关键词：柑橘三倍体有性杂交倍性分析 SSR分子标记

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Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with‘Candidatus Liberibacter asiaticus’

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Horticulture Research 2019年第1期6卷 1405-1417页

作者： Lixiao Yao Qibin Yu Ming Huang weilun Hung jude grosser Shanchun Chen Yu wang Frederick G.GmitterJr. Citrus Research Institute Southwest UniversityChongqingChina Citrus Research and Education Center University of FloridaLake AlfredFL 33850USA

Orange fruit from trees infected by‘Candidatus Liberibacter asiaticus’(CaLas)often do not look fully mature and exhibit off-flavours described as bitter,harsh,and metallic rather than juicy and *** previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods,the mechanisms leading to the off-flavour that occurs in Huanglongbing(HLB)-symptomatic fruit are not well *** this study,fruits were collected from symptomatic and healthy Valencia sweet orange(Citrus sinensis)trees grafted on Swingle(*** X Poncirus trifoliata)*** tags for relative and absolute quantification(iTRAQ)and gas chromatography-mass spectrometry(GC-MS)were used to measure the proteins,sugars,organic acids,amino acids,and volatile *** results showed that most of the differentially expressed proteins involved in glycolysis,the tricarboxylic acid(TCA)cycle and amino-acid biosynthesis were degraded,and terpenoid metabolism was significantly downregulated in the symptomatic ***,limonene,3-carene,linalool,myrcene,andα-terpineol levels were significantly lower in fruit from CaLas-infected trees than from healthy *** phenomena were observed for sucrose and *** study indicated that off-flavour of symptomatic fruit was associated with a reduction in the levels of terpenoid products and the downregulation of proteins in glycolysis,the TCA cycle,and the terpenoid biosynthesis pathway.

关键词： orange insight absolute