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甲型流感病毒感染老年小鼠支气管肺泡灌洗液中细胞因子变化与精氨酸代谢的相关性研究

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中华实验和临床病毒学杂志 2024年第4期38卷 422-431页

作者：周娟娟刘诗洋李雪杨欣欣杨俊连罗声栋陈威巍许文王福生蚌埠医科大学研究生院蚌埠233030 中国人民解放军总医院第五医学中心感染病医学部研究所北京100039 滨州医学院药学院烟台264003

目的探究精氨酸代谢在甲型流感病毒(influenza A virus,FluA)感染引起的炎症反应中的作用。方法采用FluA滴鼻感染18月龄老年小鼠,于感染后第6天采集肺组织和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)。采用Luminex多因子检... 详细信息

目的探究精氨酸代谢在甲型流感病毒(influenza A virus,FluA)感染引起的炎症反应中的作用。方法采用FluA滴鼻感染18月龄老年小鼠,于感染后第6天采集肺组织和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)。采用Luminex多因子检测法分析BALF中细胞因子水平,采用靶向代谢组学的方法分析BALF中代谢物的变化情况,并对细胞因子与代谢物之间的相关性进行分析。以FluA感染复数(multiplicity of infection,MOI)为1的剂量感染巨噬细胞,并加入不同浓度的精氨酸培养24 h。分别采用实时荧光定量RT-PCR法(real time fluorescence quantitative RT-PCR,qRT-PCR)和流式微球技术(cytometric bead array,CBA)检测白介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和IL-10的mRNA和蛋白表达量。结果与对照组相比,FluA感染组老年小鼠BALF中肺表面活性蛋白D(surfactant protein D,SP-D)、TNF-α、单核细胞趋化蛋白1(monocyte chemotactic protein-1,MCP-1)、IL-1α、IL-6、IL-10、S100钙结合蛋白(recombinant S100 calcium binding protein,S100)A9、干扰素诱导蛋白10(interferon inducible protein 10,IP-10)、巨噬细胞炎症蛋白1α(macrophage inflammatory protein-1α,MIP-1α)、基质金属蛋白酶9(matrix metalloprotein-9,MMP9)、补体因子D的水平显著升高,精氨酸代谢通路中精氨酸、天冬氨酸、瓜氨酸、谷氨酸、鸟氨酸、脯氨酸、肌酸和肌氨酸水平上调,且大部分升高的细胞因子水平与精氨酸相关代谢物呈显著正相关。FluA感染后加入精氨酸可以使巨噬细胞中IL-1β、IL-6、TNF-α的表达水平显著降低,而IL-10的水平升高。结论精氨酸可以降低FluA感染诱导的巨噬细胞炎症反应,提示其可能成为FluA感染引起的肺部重症炎症的潜在干预靶点。

关键词：甲型流感病毒支气管肺泡灌洗液细胞因子精氨酸代谢

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儿童脑脊液阳性菌株分布及耐药分析

河南医学研究 2024年第1期33卷 6-11页

作者：贾静郭明发徐璐杨俊文周娟娟杨俊梅郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院检验科河南郑州450003 郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院血液肿瘤科河南郑州450003

目的了解儿童脑脊液阳性菌株分布和常用抗菌药物的耐药情况。方法收集2017年1月至2021年12月郑州大学附属儿童医院脑脊液分离菌株,采用基质辅助激光解析电离飞行时间质谱进行病原菌鉴定,凤凰全自动细菌鉴定/药敏检测系统(Phoenix 100)、... 详细信息

目的了解儿童脑脊液阳性菌株分布和常用抗菌药物的耐药情况。方法收集2017年1月至2021年12月郑州大学附属儿童医院脑脊液分离菌株,采用基质辅助激光解析电离飞行时间质谱进行病原菌鉴定,凤凰全自动细菌鉴定/药敏检测系统(Phoenix 100)、VITEK 2 Compact全自动微生物鉴定仪(Vitek-2 Compact)和纸片扩散法进行药敏试验,按照美国临床实验室标准化协会标准判读药敏结果,用WHONET 5.6软件进行回顾性耐药性分析。结果314例脑脊液阳性病原菌以革兰阳性菌为主,204株(65.0%)。前五位病原菌依次为凝固酶阴性葡萄球菌92株(29.3%)、肠球菌44株(14.0%)、大肠埃希菌32株(10.2%)、肺炎克雷伯菌21株(6.7%)、肺炎链球菌18株(5.7%)。二、三季度分离菌株数较多,1岁以内婴儿病例最多见,占147株(46.8%)。检出科室以神经外科77株(24.5%)、新生儿内科70株(22.3%)、神经内科46株(14.6%)为主;多重耐药菌检出149株(47.5%),其中耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)69株、产超广谱β内酰胺酶肠杆菌(ESBLs)18株、耐碳青霉烯类肠杆菌(CRE)18株;各年龄组、性别、年份之间多重耐药发生率差异无统计学意义(P>0.05),不同科室之间多重耐药的发生率差异有统计学意义(P<0.001)。结论脑脊液阳性病原菌以革兰阳性菌为主,感染主要集中在1岁以内婴儿,对于多重耐药菌株应该重视耐碳青霉烯类肺炎克雷伯菌的检出。

关键词：儿童脑脊液多重耐药菌株

Assessment on System Reliability of Cable-stayed Bridges Based on Unascertained Measures

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Journal of Systems Science and Information 2008年第1期6卷 27-34页

作者： Yancang Li juanjuan Suo Shujing zhou Hebei University of Engineering Handan 056038 China

To overcome the shortcomings that there are few feasible methods and models in the comprehensive assessment on the system reliability of cable-stayed bridges, a mathematical tool-the unascertalned measure was introduced. First, the basic knowledge of the Unascertained Sets was introduced briefly. Second, the unascertained measure was defined and the comprehensive assessment model was set up. Then the method was introduced to the assessment of the system reliability of a cable-stayed bridge located in Tianjin City. Engineering practices shows that the method can complete the assessment systematically and scientifically without any assumption. The work has significance in theory and practice for the comprehensive assessment of bridge engineering.

关键词： comprehensive assessment system structural reliability cable-stayed bridge unascertalned measure information entropy

Computational Experiments for Complex Social Systems:Experiment Design and Generative Explanation

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IEEE/CAA Journal of Automatica Sinica 2024年第4期11卷 1022-1038页

作者： Xiao Xue Deyu zhou Xiangning Yu Gang Wang juanjuan Li Xia Xie Lizhen Cui Fei-Yue Wang the School of Computer Software College of Intelligence and ComputingTianjin UniversityTianjin 300350China the School of Software and the Joint SDUNTU Centre for Artificial Intelligence Research(C-FAIR) Shandong UniversityJinan 250101China the Information School Xi’an University of Financial and EconomicsXi’an 710100China the State Key Laboratory of Multimodal Artificial Intelligence Systems Institute of AutomationChinese Academy of SciencesBeijing 100190China Hainan University Haikou 570228China the State Key Laboratory for Management and Control of Complex Systems Institute of AutomationChinese Academy of SciencesBeijing 1001901China

Powered by advanced information technology,more and more complex systems are exhibiting characteristics of the cyber-physical-social systems(CPSS).In this context,computational experiments method has emerged as a novel approach for the design,analysis,management,control,and integration of CPSS,which can realize the causal analysis of complex systems by means of“algorithmization”of“counterfactuals”.However,because CPSS involve human and social factors(e.g.,autonomy,initiative,and sociality),it is difficult for traditional design of experiment(DOE)methods to achieve the generative explanation of system *** address this challenge,this paper proposes an integrated approach to the design of computational experiments,incorporating three key modules:1)Descriptive module:Determining the influencing factors and response variables of the system by means of the modeling of an artificial society;2)Interpretative module:Selecting factorial experimental design solution to identify the relationship between influencing factors and macro phenomena;3)Predictive module:Building a meta-model that is equivalent to artificial society to explore its operating ***,a case study of crowd-sourcing platforms is presented to illustrate the application process and effectiveness of the proposed approach,which can reveal the social impact of algorithmic behavior on“rider race”.

关键词： Agent-based modeling computational experiments cyber-physical-social systems(CPSS) generative deduction generative experiments meta model

Linear Quadratic Optimal Control for Systems Governed by First-Order Hyperbolic Partial Differential Equations

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Journal of Systems Science & Complexity 2024年第1期37卷 230-252页

作者： XUE Xiaomin XU juanjuan ZHANG Huanshui School of Control Science and Engineering Shandong UniversityJinan 250061China College of Electrical Engineering and Automation Shandong University of Science and TechnologyQingdao 266000China

This paper focuses on linear-quadratic(LQ)optimal control for a class of systems governed by first-order hyperbolic partial differential equations(PDEs).Different from most of the previous works,an approach of discretization-then-continuousization is proposed in this paper to cope with the infinite-dimensional nature of PDE *** contributions of this paper consist of the following aspects:(1)The differential Riccati equations and the solvability condition of the LQ optimal control problems are obtained via the discretization-then-continuousization method.(2)A numerical calculation way of the differential Riccati equations and a practical design way of the optimal controller are ***,the relationship between the optimal costate and the optimal state is established by solving a set of forward and backward partial difference equations(FBPDEs).(3)The correctness of the method used in this paper is verified by a complementary continuous method and the comparative analysis with the existing operator results is *** is shown that the proposed results not only contain the classic results of the standard LQ control problem of systems governed by ordinary differential equations as a special case,but also support the existing operator results and give a more convenient form of computation.

关键词： Discretization-then-continuousization method first-order hyperbolic partial differential equations forward and backward partial difference equations linear quadratic optimal control.

Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation

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Journal of Genetics and Genomics 2020年第5期47卷 249-261页

作者： Xiaomin Wang Hui Shi juanjuan zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo Zhen Ouyang Chengdan Lai Quanmei Yan Liangxue Lai CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China University of Chinese Academy of Sciences Beijing100049China Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL) Guangzhou510005China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing100101China Research Unit of Generation of Large Animal Disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou510530China School of Biotechnology and Health Sciences Wuyi UniversityJiangmen529020China Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational MedicineShenzhen University Health Science CenterShenzhen University School of MedicineFirst Affiliated Hospital of Shenzhen UniversityShenzhen Second People's HospitalShenzhen518035China Department of Central Laboratory Shenzhen Longhua District Central HospitalShenzhen518110China School of Life Sciences University of Science and Technology of ChinaHefei230027China Joint School of Life Sciences Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou511436China

Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted ***,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is *** this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1 protein,which is associated with endothelial and hematopoietic cell *** found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse *** whole yolk sac(YS)of Flk-1^EGFP/ECFP rat-mouse chimera was full of rat blood *** genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the *** results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse.

关键词： Blastocyst complementation Interspecies chimera Intraspecies chimera Flk-1 Vascular endothelial cell Hematopoietic cell

Energy transfer, anticounterfeiting, white light emission and sensing in fine-regulating series of lanthanide metal-organic frameworks

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Journal of Rare Earths 2024年第3期42卷 446-454,I0001页

作者： juanjuan Xue Yao Wang Guoping Yang Yaoyu Wang Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education Shaanxi Key Laboratory of Physico-Inorganic ChemistryCollege of Chemistry&Materials ScienceNorthwest UniversityXi'an710127China

Three lanthanide ions and a rigid(1,1'-biphenyl)-2,4,6-tricarboxylic acid(H_(3)bptc) ligand led to the isostructural lanthanide metal-organic frameworks(Ln-MOFs) {[Ln(bptc)(H_(2)O)_(3)]·3H_(2)O}_n(Lnbptc, Ln = Eu,Tb, Gd, Eu_(x)Tb_(1—x), Eu_(x)Gd_(1-x), Tb_(x)Gd_(1-x), and Eu_(0.12)Tb_(0.52)Gd_(0.36)). The density functional theory(DFT) and time-dependent DFT calculation were expanded on H_3bptc, which point to the occurrence of “antenna effect”. The minor diameter samples of Eubptc, Tbbptc, and Gdbptc can also be attained via the microwave synthesis and employed for handwriting anticounterfeiting. Interestingly, the typical red emission of Eu^(3+) and green emission of Tb~(3+) could be fine-tuned by Gd^(3+) via chemical mixing to be full-color emissions bimetallic doped Eu_(x)Tb_(1-x)bptc, Eu_(x)Gd_(1-x)bptc, and Tb_(x)Gd_(1-x)bptc. The above synthetic bimetallic MOFs can be fixed in positioning boxes of quick response(QR) codes with diverse emission for anticounterfeiting. The message can be decoded under the irradiation of 365 nm ultraviolet lamp while invisible in the daylight. Notably, the trimetallic doped Eu_(0.12)Tb_(0.52)Gd_(0.36)bptc emits white light with the CIE color coordinate(0.34, 0.34). Furthermore, Tbbptc is demonstrated to be a sensitive probe toward nitrobenzene and acetone with multiple mechanisms.

关键词： Metal-organic frameworks Anticounterfeiting White light-emitting Sensing Rare earths

Recent Advances in C-F Bond Formation from Carbon-Centered Radicals

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Chinese Journal of Chemistry 2024年第9期42卷 1009-1031页

作者： Jihua Zhang juanjuan Wang Qiang Cheng College of Chemistry and Molecular Sciences Wuhan UniversityWuhanHubei 430072China

Construction of C-F bonds is a direct and efficient method for introducing fluorine into pharmaceuticals,agrochemicals,and *** such as nucleophilic,electrophilic,radical,and transition-metal catalyzed fluorination have been developed to meet the demand of diverse C-F bond *** them,radical fluorination has been witnessed with substantial advancement in a recent ***,we reviewed methods for formation of C-F bonds with carbon-centered radicals as key intermediates,especially in recent five *** introduce in the paper with different fluorinating reagents,strategies for radical generation,and application in late-stage functionalization and synthesis of PET *** also indicate the current limitations and propose the direction of the field for the future development.

关键词： Fluorine Fluorinating reagents Late-stage fluorination [^(18)F]jfluorination Radical reactions Synthetic methods

Mechanism of PARP inhibitor resistance and potential overcoming strategies

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Genes & Diseases 2024年第1期11卷 306-320页

作者： Xiaoyu Fu Ping Li Qi zhou Ruyuan He Guannan Wang Shiya Zhu Amir Bagheri Gary Kupfer Huadong Pei juanjuan Li Department of Oncology Georgetown Lombardi Comprehensive Cancer CenterGeorgetown University Medical CenterWashingtonDC 20057USA Department of Breast and Thyroid Surgery Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Cancer Center Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Thoracic Surgery Renmin Hospital of Wuhan UniversityWuhanHubei 430060China

PARP inhibitors(PARPi)are a kind of cancer therapy that targets poly(ADP-ribose)*** is the first clinically approved drug to exert synthetic lethality by obstruct-ing the DNA single-strand break repair *** the significant therapeutic effect in pa-tients with homologous recombination(HR)repair deficiency,innate and acquired resistance to PARPi is a main challenge in the *** this review,we mainly discussed the underlying mechanisms of PARPi resistance and summarized the promising solutions to overcome PARPi resistance,aiming at extending PARPi application and improving patient outcomes.

关键词： Drug resistance Homologous recombination PARP PARP inhibitor Poly(ADP-ribose)polymerase