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STRUCTURE,MOLECULAR WEIGHT AND BIOACTIVITIES OF(1→3)-β-D-GLUCANS AND ITS SULFATED DERIVATIVES FROM FOUR KINDS OF LENTINUS EDODES

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Chinese Journal of Polymer Science 2005年第3期23卷 327-336页

作者： UnursaikhanSurenjav 张俐娜 Xiao-juanxu MeiZhang PeterChiKeungCheung Fan-boZeng DepartmentofChemistry WuhanUniversityWuhan430072China DepartmentofBiological TheChineseUniversityofHongKongHongKongChina TongjiMedicalCollege HuazhongUniversityofScienceandTechnologyWuhan430030China

Lentinan samples,(1→3)-β-D-glucans containing 4.6-15.2 wt% proteins,coded as L-I_1 L-I_2 L-I_3 and L-I_4(L-I)were isolated from four kinds of Lentinus *** glucans were treated with acetone to remove the protein in orderto obtain free protein glucans coded as LNP-I_1,LNP-I_***-I_3 and LNP-I_4(LNP-I).The free-protein polysaccharideswere sulfated to give derivatives(S-LNP-I)with degree of substitution(DS)from *** structural features andweight-average molecular weight(M_w)of the samples were investigated by using infrared spectroscopy,elemental analysis,^(13)C-NMR,size exclusion chromatography combined with laser light scattering(SEC-LLS)and *** effects ofstructure and conformation of the polysaccharides on antitumor activities were assayed in vivo(Sarcoma 180 solid tumors)and in vitro(Sarcoma 180,HL-60,MCF-7 and Vero tumors).The results indicated that the predominant species of thesamples L-I and LNP-I in 0.2 mol/L NaCl aqueous solution existed as triple-helical chains with high rigidity and in dimethylsulfoxide(DMSO)as single-flexible ***,the antitumor activities of LNP-I are lower than those of the nativeglucans(L-I),whereas their sulfated derivatives have higher inhibition ratio against Sarcoma 180 than *** resultsreveal that the binding of protein,sulfated modification and the triple helix conformation are important factors in theenhancement of the antitumor activities of polysaccharides on the whole.

关键词： (1→3)-β-D-glucans Lentinus Edodes Molecular weight Protein Sulfated derivative Antitumor activity.

EFFECTS OF RETINOIC ACID ON PROLIFERATION AND DIFFEREN-TIATION OF A HUMAN OVARIAN CARCINOMA CELL LINE:3AO

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Chinese Medical Sciences Journal 2005年第1期20卷 51-54,页

作者： Ming-juanxu YingCui NingHui Yu-JianLiu DepartmentofObstetricsandGynecology ChanghaiHospitalSecondMilitaryMedicalUniversityShanghai200433

Objective To observe the effects of retinoic acid (RA) on the proliferation and differentiation of a human ovarian carcino-ma cell line: 3AO cells. Methods 3AO cell proliferation was evaluated by viable cell count, percentage of cells in each cycle phase were analyzed by flow cytometric analysis, alkaline phosphatase (AKP) activity was determined as described , and CA125 expression was measured by ELISA. Results RA could inhibit the proliferation of 3AO cells accompanied with morphological changes in a dose-dependent manner. Cell cycle analysis indicated that RA inhibition of 3AO cells growth occurred through induction of G1 arrest with a concomitant reduction in the proportion of cells in S phase, AKP activity increased significantly after treatment with RA(0.1 μmol/L) for 1-5 days. Dose-response studies revealed that the AKP activity increased to a different extent as a function of RA concentrations. Furthermore, RA could suppress the expression of CA125 tumor marker in 3AO *** RA could markedly inhibit the proliferation and induce the differentiation of 3AO cells. for 1-5 days. Dose

关键词： retinoic acid ovarian carcinoma alkaline phosphatase tumor marker antigen

Study of Benign and Malignant Intraductal Lesions of the Breast by Fiberoptic Ductoscopy

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Chinese Journal of Clinical Oncology 2005年第1期2卷 529-532页

作者： juanxu QiWang AnqinZhang WenpingLi JuntaoShi ZhongyangChen BreastCenteroftheGuangdongMatemalandChildren＇sHospital GuangzhouMedicalCollegeGuangzhou510010China BreastCenteroftheGuangdongMatemalandChildren＇sHospital GuangzhouMedicalCollegeGuangzhou510010China

OBJECTIVE To observe and subtype the appearance of intraductal papilloma (lesions) and of infiltrating ductal carcinoma or early infiltrating ductal carcinoma using a fiberoptic ductoscope (FDS) examination, and to discuss the differentiation and diagnosis of benign and malignant tumors,by *** The characteristics of FDS images and diagnostic data for 229 patients with intraductal papilloma (lesions) and 50 patients with ductal carcinoma, who were confirmed by surgical pathology from October 1998 to December 2003, were analyzed *** The appearance of the lesions observed by FDS were grouped into 4types: a monothelia (type Ⅰ), polythelia (type Ⅱ), superficies (type Ⅲ) and a mixture (type Ⅳ). Intraductal papillomas (lesions) were more commonly seen in type Ⅰ and Ⅱ, and intraductal carcinomas, or early infiltrating ductal carcinomas were more commonly seen in type Ⅲ and Ⅳ;there was a statistically significant difference in the distribution of the ductoscopic types, except in type Ⅱ, between the two types of lesions, P<0.001. The focal detection rate by FDS for intraductal papilloma and papillomatosis was 99.6% (228/229) and for breast cancer was 96.0% (48/50). The diagnostic accuracy was 97.8% (224/229) and 82.0% (41/50),*** FDS can be a guide for the treament of benign and malignant intraductal tumors, with early discovery and accurate diagnosis.

关键词：管内乳突淋瘤胸腺检查诊断方法纤维支气管镜

PhGRL2 Protein, Interacting with PhACO1, Is Involved in Flower Senescence in the Petunia

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Molecular Plant 2014年第8期7卷 1384-1387页

作者： Yinyan Tan, juanxu Liu, Fang Huang, Jiefu Guan, Shan Zhong, Na Tang, Ji Zhao, Weiyuan Yang Yixun Yu College of Horticulture South China Agricultural University Guangzhou 510642 China

Dear Editor, The pathways of ethylene biosynthesis and signaling have been studied in detail （Ji and Guo, 2013）. Arabidopsis REVERSION-TO-ETHYLENE SENSITIVITY1 （RTE1）, interacting with ETR1, and its homologs tomato GREEN RIPE （SlGR） and SlGRL1, and rice OsRTH1 negatively regulate the ethylene signaling （Barry and Giovannoni, 2006, Resnick et al., 2006; Dong et al., 2010; Zhang et al., 2012）. A newly published study suggested that a cytochrome b5 and RTE1 are functional partners in promoting ETRl-mediated repression of ethylene signaling in Arabidopsis （Chang et al., 2014）. However, AtRTH, RTE1 homolog in Arabidopsis, and its closest homolog in the tomato, SlGRL2 （GR-like2）, do not play a role in ethylene signaling （Dong et al., 2010）, and the function of the homologs of these members is not well known.

关键词： PLANT proteins ETHYLENE CELLULAR aging PETUNIAS PLANT gene expression MAGNOLIOPSIDA

Uridine adenosine tetraphosphate acts as a pro-angiogenic factor in vitrothrough purinergic P2Y receptors

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中国药理学与毒理学杂志 2015年第S1期29卷 114-114页

作者： Zhi-chaoZHOU IhsanCHRIFI Yan-juanxu DirkJDUNCKER SJamalMUSTAFA DaphneMERKUS CarolineCHENG Division of Experimental Cardiology Cardiovascular Research School COEUR Erasmus MC University Medical Center Rotterdam Molecular Cardiology Department of Cardiology Thoraxcenter Laboratory of Renal and Vascular Biology Department of Nephrology &Hypertension University Medical Center Utrecht Department of Physiology and Pharmacology Center for Cardiovascular and Respiratory Sciences West Virginia University

OBJECTIVE Uridine adenosine tetraphosphate(Up4A),a dinucleotide,contains both purine and pyrimidine moieties,and exerts its vascular influence via activation of purinergic ***,we aimed to investigate the effects of Up4 A on angiogenesis and the putative purinergic receptors(PR)involved in this *** Tubule formation assay was performed in 3D matrix *** this assay,human umbilical vein endothelial cells(HUVECs)were co-cultured with pericytes with various Up4 A doses(0,1,2.5,5,10 and 20μmol·L-1)in the absence and presence of P2Y6 R antagonist MRS2578(10μmol·L-1)for *** profile of PR subtypes and angiogenic factors was assessed in HUVECs by q-PCR with and without P2Y6 R *** No difference in initial tubule formation was detected between Up4 A stimulation and control conditions at day *** contrast,a significant increase in vascular density in response to Up4 A was observed at day ***4 A at a dose of 2.5and 5μmol·L-1 promoted total tubule length(by-1.89 fold and-2.23fold),number of tubules(by-1.71 fold and-1.89fold)as well as number of junctions(by-2.24 fold and-2.80fold),all of which were inhibited by *** increase in Up4 A dose to10 and 20μmol·L-1 did not induce an increase in these vascular parameters as compared to non-treated ***,Up4 A increased mRNA level of P2YRs(P2Y2R,P2Y4 R and P2Y6R)but not P2XR(P2X4R and P2X7R)or P1R(A2AR and A2BR),while Up4 A upregulated VEGFA and ANGPT1 but not VEGFR2,ANGPT2,Tie1 and Tie2at mRNA *** upregulation of P2 YRs and angiogenic factors by Up4 A was inhibited by *** Up4 A is functionally capable of promoting tubule formation in vitro co-culture *** process is likely mediated by activation of pyrimidine-favored P2 YRs but not P2 XR or P1 Rs,and involves stimulation of well known angiogenic factors.

关键词： Up4A purinergic receptors angiogenesis P2Y6 tubule

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香石竹植株再生及基因工程研究进展

植物学通报 2006年第1期23卷 23-28页

作者：余义勋刘娟旭包满珠华南农业大学园艺学院广州510642 华中农业大学园艺林学学院园艺植物生物学教育部重点实验室武汉430070

本文对香石竹的再生体系、遗传转化体系及其分子育种现状作了较为系统的总结。香石竹的再生体系多以器官直接再生不定芽为主,而通过愈伤组织和体细胞胚途径再生报道较少。用农杆菌介导法和基因枪法均可建立香石竹遗传转化体系,但近年来... 详细信息

本文对香石竹的再生体系、遗传转化体系及其分子育种现状作了较为系统的总结。香石竹的再生体系多以器官直接再生不定芽为主,而通过愈伤组织和体细胞胚途径再生报道较少。用农杆菌介导法和基因枪法均可建立香石竹遗传转化体系,但近年来的研究显示农杆菌介导法应用普遍且比较稳定。近年来以延长香石竹瓶插寿命为目标的分子育种研究已取得较大进展,对其色、香和形等其他重要性状的分子育种也已经起步,而有关香石竹抗性的分子育种有待进一步开拓。

关键词：香石竹植株再生遗传转化