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Uncovering the genetic diversity of Giardia intestinalis in isolates from outbreaks in New Zealand

Infectious Diseases of Poverty 2022年第3期11卷 27-36页

作者： Paul Ogbuigwe Patrick J.Biggs juan carlos garcia-ramirez Matthew A.Knox Anthony Pita Niluka Velathanthiri Nigel P.French David T.S.Hayman School of Veterinary Science Massey UniversityPalmerston NorthManawatu-WanganuiNew Zealand School of Natural Sciences Massey UniversityPalmerston NorthManawatu-WanganuiNew Zealand

Background:Giardia intestinalis is one of the most common causes of diarrhoea worldwide. Molecular techniques have greatly improved our understanding of the taxonomy and epidemiology of this parasite. Co-infection with mixed (sub-) assemblages has been reported, however, Sanger sequencing is sometimes unable to identify shared subtypes between samples involved in the same epidemiologically linked event, due to samples showing multiple dominant subtypes within the same outbreak. Here, we aimed to use a metabarcoding approach to uncover the genetic diversity within samples from sporadic and outbreak cases of giardiasis to characterise the subtype diversity, and determine if there are common sequences shared by epidemiologically linked cases that are missed by Sanger ***:We built a database with 1109 unique glutamate dehydrogenase (gdh) locus sequences covering most of the assemblages of G. intestinalis and used gdh metabarcoding to analyse 16 samples from sporadic and outbreak cases of giardiasis that occurred in New Zealand between 2010 and ***:There is considerable diversity of subtypes of G. intestinalis present in each sample. The utilisation of metabarcoding enabled the identification of shared subtypes between samples from the same outbreak. Multiple variants were identified in 13 of 16 samples, with Assemblage B variants most common, and Assemblages E and A present in mixed ***:This study showed that G. intestinalis infections in humans are frequently mixed, with multiple subtypes present in each host. Shared sequences among epidemiologically linked cases not identified through Sanger sequencing were detected. Considering the variation in symptoms observed in cases of giardiasis, and the potential link between symptoms and (sub-) assemblages, the frequency of mixed infections could have implications for our understanding of host–pathogen interactions.

关键词： DNA sequencing Epidemiology Genetic diversity Giardiasis Metabarcoding

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Transformation on CdCO3？CdO Thin Films under Annealing in Air Atmosphere

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材料科学与工程（中英文A版） 2013年第5期3卷 289-298页

作者： Ruth Melendrez Luevano Tania Flores Papaqui juan carlos ramirez garcia Marcial Zamora Tototzintle Jorge R. Cerna Melissa Chivez Portillo Salvador Rosas Castilla Oscar Portillo Moreno School of Sciences Chemical Autonomous University of Puebla Puebla 72001 M~xico CIDS-ICUAP AutonomousUniversity of Puebla 72001 Puebla Mdxico

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Navigating medial patellotibial ligament reconstruction:Clinical perspectives and surgical strategies

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World Journal of Clinical Cases 2025年第1期13卷 6-10页

作者： juan Pablo Zicaro Ignacio garcia-Mansilla Division of Knee Hospital Italiano de Buenos AiresBuenos Aires 1109Argentina

The surgical approach for patellar instability usually refers to reconstruction of the medial patellofemoral ligament associated with an osteotomy of the tibial tuberosity or a trochleoplasty when *** medial patellotibial ligament and the medial patellomeniscal ligament are secondary stabilizers of the *** this,both the medial patellotibial and patellofemoral ligaments aid in patellar rotation and tilt when the knee is flexed beyond 45°.The medial patellotibial ligament plays a particularly important role in the final stages of stretching in extension and between 40 degrees to 90 degrees of *** clinical relevance and surgical indications for medial patellotibial ligament reconstruction associated with medial patellofemoral ligament reconstruction are still *** editorial explores the surgical indications and clinical results for medial patellotibial ligament reconstruction to improve readers’understanding of this technique,especially because reported clinical outcomes have remained sparse.

关键词： Medial patellotibial ligament Patellar instability Reconstruction Surgical

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Assessment of effectiveness of oral supplementation of isolated fiber of carrot on metabolic parameters in mature rats

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Food Science and Human Wellness 2023年第6期12卷 2022-2028页

作者： Maria Rosana ramirez Debora Manuale juan carlos Yori Instituto Universitario de Ciencias de la Salud Fundación BarcelóCONICETCenteno 710CP 3340Santo TomeCorrientesArgentina Consejo Nacional de Investigaciones Científi cas Técnicas(CONICET) Buenos Aires C1033AAJArgentina Instituto de Investigaciones en Catálisis y Petroquímica(INCAPE)FIQ-UNL-CONICET Parque Tecnológico Litoral Centro 3000Santa FeArgentina Facultad de Ingeniería Química Universidad nacional del Litoral 3000Santa FeArgentina

This present study was conducted to evaluate the metabolic effects of isolated fi ber of carrot supplementation in *** properties of fi ber were *** groups were as follows:animals fed a standard diet,control group;high fi ber supplementation(70 mg);low fi ber supplementation(35 mg);for 12 *** samples were collected at the time of sacrifi *** weights of heart,liver,kidneys and spleen of the experimental rats with respect to body weight were *** kits were used to determine serum glucose concentration,lipid profi le(cholesterol,HDL-cholesterol,triglycerides),and the two main aminotransferases glutamic-oxalacetic transaminase(GOT)/glutamate-pyruvate transaminase(GPT).A histopathological assay was performed on the heart,liver,and spleen tissues of *** with fi ber favors weight loss in female((242.03±23.73)-(197.81±10.45)g);and male rats((262.50±32.21)-(213.96±12.56)g and induces a decrease in glucose levels in the supplemented *** the exception of total high-density lipoprotein cholesterol,the other lipid fractions decrease signifi cantly in rats *** supplementation did not induce changes in the dissected organs of the supplemented *** conclusion supplementation of fiber,improves glucose control,lower plasma lipid concentrations and reduced body weight in normal rats.

关键词： Carrot fiber Oral supplementation Animal studies

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Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

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Plant Diversity 2017年第1期39卷 13-19页

作者： Reyna Maya-garcia Santiago Arizaga Pablo Cuevas-Reyes juan Manuel Penaloza-ramirez Victor Rocha ramirez Ken Oyama Facultad de Biologia Universidad Michoacana de San Nicolas de Hidalgo Mexico Escuela Nacional-de Estudios Superiores Unidad Morelia UNAM Antigua Carretera a Pdtzcuaro 8701 Colonia Ex-Hacienda de SanJose de la Huerta58190 Morelia Michoacan Mexico

Mammillaria pectinifera is an endemic, short-globose cactus species, included in the IUCN list as a threatened species with only 18 remaining populations in the Tehuacfin-Cuicatlan Valley in central Mexico. We evaluated the population genetic diversity and structure, connectivity, recent bottlenecks and population size, using nuclear microsatellites. M. pectinifera showed high genetic diversity but some evidence of heterozygote deficiency （F15）, recent bottlenecks in some populations and reductions in population size. Also, we found low population genetic differentiation and high values of connectivity for M. pectinifera, as the result of historical events of gene flow through pollen and seed dispersal. M. pectinifera occurs in sites with some degree of disturbance leading to the isolation of its populations and decreasing the levels of gene flow among them. Excessive deforestation also changes the original vegetation damaging the natural habitats. This species will become extinct if it is not properly preserved. Furthermore, this species has some ecological features that make them more vulnerable to disturbance such as a very low growth rates and long life cycles. We suggest in situ conservation to prevent the decrease of population sizes and loss of genetic diversity in the natural protected areas such as the Tehuacan-Cuicatlan Biosphere Reserve. In addition, a long-term ex situ conservation program is need to construct seed banks, and optimize seed germination and plant establishment protocols that restore disturbed habitats. Furthermore, creating a supply of living plants for trade is critical to avoid further extraction of plants from nature.

关键词： MammiUaria pectiniferaNuclear SSRGenetic diversityRecent bottlenecksEffective population size

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Changes in culm surface temperature with maturity of the bamboo species Guadua angustifolia

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Journal of Forestry Research 2016年第2期27卷 419-425页

作者： Nils Nolke juan carlos Camargo garcia Christoph Klein Andrea Polle Chair of Forest Inventory and Remote Sensing Universitat GottingenBusgenweg 537077 GottingenGermany Faculty of Environmental Sciences Technological Universityof PereiraLa JulitaPereiraColombia Department of Forest Botany and Tree Physiology Universiat GottingenBiJsgenweg 237077 GottingenGermany

We used thermal images of bamboo culms of Guadua angustifolia Kunth to analyze the relationship between culm surface temperature and maturity, driven by the hypothesis that young culms may exhibit lower surface temperatures than old ones. The culm surface temperature shows small but constant differences between three age classes of 1, 2, and 3 years. Our findings indicate that surface temperature may be applied as an additional indi- cator to support the determination of maturity of guadua culms besides the visual assessment of the culms.

关键词： Bamboo culms Thermal images Bamboowood quality

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Topological Minors in Bipartite Graphs

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Acta Mathematica Sinica,English Series 2011年第11期27卷 2085-2100页

作者： Camino BALBUENA Martin CERA Pedro garcia-VAZQUEZ juan carlos VALENZUELA Departament de Matematica Aplicada III Universitat Politecnica de Catalunya C/ Jordi Girona 1-3 （ Edifici C2 Despatx 302） 08034 Barcelona Spain Departamento de Matemdtica Aplicada I Universidad de SeviUa EUIT Agrlcola Ctra. Utrera Kin. 1 41013 Sevilla Spain Departamento de Matemdtica Aplicada I Universidad de Sevilla ETS Arquitectura Avda. Reina Mercedes 2 41012 Sevilla Spain Departamento de Matemdticas Universidad de Cddiz EPS Algeciras Avda. Ramdn Puyol s/n 11202 Algeciras Spain

For a bipartite graph G on m and n vertices, respectively, in its vertices classes, and for integers s andt such that 2≤ s ≤ t, 0≤ m-s ≤ n-t, andre＋n≤ 2s＋t-1, we prove that if G has at least mn- （2（m - s）＋ n - t） edges then it contains a subdivision of the complete bipartite K（s,t） with s vertices in the m-class and t vertices in the n-class. Furthermore, we characterize the corresponding extremal bipartite graphs with mn- （2（m - s）＋ n - t ＋ 1） edges for this topological Turan type problem.

关键词： Bipartite graphs extremal graph theory topological minor

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Influence of V(C,N) Precipitates on Microstructure and Mechanical Properties of Continuous Cooled C-Mn-V

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Journal of Iron and Steel Research International 2011年第S1期18卷 266-270页

作者： carlos garcia-Mateo carlos Capdevila juan Cornide Jesus Chao Francisca G Caballero carlos garcia de Andres Materalia Research Group Department of Physical Metallurgy Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC)Avda.Gregorio del Amo8 E-28040 MadridSpain

The effect of chemical composition and processing parameters on the formation of acicular ferrite and/or bainite has been *** particular,this paper deals with the influence that N through its combination with V,as V(C,N) precipitates,has on the decomposition of ***,the intragranular nucleation potency of V(C,N) precipitates is analyzed through the continuous cooling transformation diagrams (CCT) of two C-Mn-V steels with different contents of *** reported in this work allow us to conclude that acicular ferrite can only be achieved alloying with vanadium and nitrogen,meanwhile bainite is promoted in steels with a low level of *** is concluded that higher strength values are obtained in acicular ferrite than in bainitic steel but a similar brittle-ductile transition temperature (BDT),and lower values of impact absorbed energy (KV) has been recorded in nitrogen-rich steel.

关键词： acicular ferrite bainite vanadium precipitates mechanical properties

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Smart Contract to Traceability of Food Social Selling

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Computers, Materials & Continua 2023年第3期74卷 4703-4728页

作者： Cristian Valencia-Payan JoséFernando Grass-Ramírez Gustavo ramirez-Gonzalez juan carlos Corrales University of Cauca Calle 5#4-70190003Popayan-CaucaColombia

Traditionally,food sustainability has been considered solely in the stage of agricultural ***,globalization,the expansion of the food production industry,and the emergence of supermarket chains that control the retail food market require specific significant changes in supply chains in the food sector and,therefore,we need to address the economic,social,and environmental impacts of these *** the other hand,social selling has increased rapidly in recent years,with a further boom,following current events related to the coronavirus disease(COVID-19).This explosion of social sales,where there are usually no control and regulation entities,can bring problems associated with mishandling *** this paper,we expose how Blockchain technology supports the traceability of social sales by validating the data provided by the chain participants such as digital health passports,production and transport data in the sale process;the proposed solution generates recommendations on productmanagement considering the agreements previously made by the network *** evaluate the proposed smart contracts,we useHyperledger Caliper,obtaining an average throughput of 12.6 transactions per second and an average latency of 0.3 s for the asset update *** also use a study case to evaluate the proposed project platform’s selling-transport stage using Internet of Things(IoT)sensors.

关键词： Blockchain reliability smart contracts social selling traceability artisan sweets

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Bacterial extracts and bioformulates as a promising control of fruit body rot and root rot in avocado cv. Hass

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Journal of Integrative Agriculture 2020年第3期19卷 748-758页

作者： David GRANADA Lorena LÓPEZ-LUJAN Sara ramirez-RESTREPO juan MORALES carlos PELÁEZ-JARAMILLO Galdino ANDRADE juan carlos BEDOYA-PEREZ Phytosanitary and Biological Control Unit Corporation for Biological ResearchMedellin 050034Colombia Faculty of Health Sciences Institution University Colegio Mayor de AntioquiaMedellin 050034Colombia Department of Agronomic Sciences National University of ColombiaMedellin 050034Colombia Faculty of Exact Sciences University of AntioquiaMedellin 050010Colombia Department of Microbiology Londrina State UniversityLondrina 86051-990Brazil

At least 20-40% of annual losses of avocado crops are caused by pathogenic *** chemical treatments of these diseases are inefficient,cause environmental pollution and are increasingly restricted by international *** work aimed to assess the biocontrol capacity of a bacterial extract to protect avocado fruits and plants from pathogen *** from the bacterial isolate Serratia ***5.1 were obtained from liquid fermentations in a biorreactor.A body rot postharvest infection model with Colletotrichum gloeosporioides on fruits was ***,packaging conditions were simulated using the bacterial extract and the commercial fungicide prochloraz as a positive ***,seedlings infections with Phytophthora cinnamomi were performed on two types of avocado(West Indian race and ***).The Area Under Disease Progress Curve(AUDPC) was recorded using the bacterial extract and a commercial product with fosetyl-aluminium as *** bacterial extract significantly reduced infections by *** on injured avocado fruits at 31.1 μg mL^-*** fruits were also protected against body rot infections at the same concentration and showed no significant differences with the commercial *** the other hand,AUDPC in the seedlings was significantly reduced with the extract treatment at 3 μg mL^-1 compared to the ***,a possible phytotoxicity effect of the extract was evidenced in the seedlings and confirmed by pathogen recovery and tests on Raphanus sativus ***,formulations of the extracts(emulsion and emulsifiable concentrate) were prepared,and bioactive stability was assessed for 8 *** emulsion formulates demonstrated very stable bioactivity against *** extract and the emulsion formulate showed promising results for the control of avocado *** bioproducts based on this type of active principles could be developed for the benefit of avocado industry.

关键词： Colletotrichum gloeosporioides Phytophthora cinnamomi Serratia sp. antagonistic microbes secondary metabolites biofungicide

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