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A novel mouse model of calcific aortic valve stenosis

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Animal Models and Experimental Medicine 2024年第4期7卷 523-532页

作者： Ningjing Qian Yaping Wang Wangxing Hu Naifang cao Yi Qian Jinyong chen juan Fang Dilin Xu Haochang Hu Shuangshuang Yang Dao Zhou Hanyi Dai Dongdong Wei Jian'an Wang Xianbao Liu Department of cardiology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina State Key Laboratory of Transvascular Implantation Devices China cardiovascular Key Laboratory of Zhejiang Province HangzhouChina Department of cardiovascular Surgery The Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina Department of Endocrinology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina Binjiang Institute of Zhejiang University HangzhouChina

Background:calcific aortic valve stenosis(cAVS)is one of the most challenging heart diseases in clinical with rapidly increasing ***,study of the mecha-nism and treatment of cAVS is hampered by the lack of suitable,robust and efficient models that develop hemodynamically significant stenosis and typical calcium ***,we aim to establish a mouse model to mimic the development and features of ***:The model was established via aortic valve wire injury(AVWI)combined with vitamin D subcutaneous injected in wild type c57/BL6 *** transthoracic echocardiography was applied to evaluate aortic jet peak velocity and mean *** specimens were collected and examined in respect of valve thickening,calcium deposition,collagen accumulation,osteogenic differentiation and ***:Serial transthoracic echocardiography revealed that aortic jet peak velocity and mean gradient increased from 7 days post model establishment in a time depend-ent manner and tended to be stable at 28 *** with the sham group,sim-ple AVWI or the vitamin D group,the hybrid model group showed typical pathological features of cAVS,including hemodynamic alterations,increased aortic valve thicken-ing,calcium deposition,collagen accumulation at 28 *** addition,osteogenic dif-ferentiation,fibrosis and inflammation,which play critical roles in the development of cAVS,were observed in the hybrid ***:We established a novel mouse model of cAVS that could be induced efficiently,robustly and economically,and without genetic *** provides a fast track to explore the underlying mechanisms of cAVS and to identify more effec-tive pharmacological targets.

关键词： animal model calcific aortic valve stenosis valve calcification

印太经济框架“供应链协议”的特征、趋势及中国应对

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上海对外经贸大学学报 2024年第3期31卷 5-16,88页

作者：常丽娟上海WTO事务咨询中心上海200336

“供应链协议”是印太经济框架(IPEF)下达成的首个协议,为印太地区各缔约方参与到由美国主导的供应链重塑进程,提供了基本合作框架。不同于RcEP、cPTPP等传统自由贸易协议,IPEF“供应链协议”具有非约束性、显著排他性、执行机制灵活性... 详细信息

“供应链协议”是印太经济框架(IPEF)下达成的首个协议,为印太地区各缔约方参与到由美国主导的供应链重塑进程,提供了基本合作框架。不同于RcEP、cPTPP等传统自由贸易协议,IPEF“供应链协议”具有非约束性、显著排他性、执行机制灵活性、劳工标准创新性等贸易制度新特征。IPEF“供应链协议”反映出拜登政府正推行与其产业政策协同的贸易新方法,其效用评估将重点取决于美国的单边举措。IPEF“供应链协议”的实施预计将加速推动全球供应链体系和生产布局的调整。同时,该协议也将在供应链转移、新兴竞争领域、劳工标准等贸易规则的话语权争夺方面,对中国提出挑战。为应对这一挑战,中国应予以高度重视,并通过强化多边合作、优化自身供应链、推动劳工改革以及积极参与国际规则制定等方式来应对IPEF“供应链协议”所带来的挑战。

关键词：印太经济框架劳工标准供应链韧性规则重构

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基于强弱感知设计的驾驶员危险感知状态识别模型研究

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汽车工程 2024年第6期46卷 995-1005页

作者：曾娟王昊许博张洪昌武汉理工大学现代汽车零部件技术湖北省重点实验室武汉430070 武汉理工大学重庆研究院重庆401135

驾驶员危险感知能力对于预防和减少道路交通事故具有重要作用。针对目前研究中危险感知的特征向量表征不统一、算法对实际问题可解释性不足的弊端,通过人为控制的方式,设计了基于危险源、显隐性、强弱危险感知状态3个维度的3×2×2实验... 详细信息

驾驶员危险感知能力对于预防和减少道路交通事故具有重要作用。针对目前研究中危险感知的特征向量表征不统一、算法对实际问题可解释性不足的弊端,通过人为控制的方式,设计了基于危险源、显隐性、强弱危险感知状态3个维度的3×2×2实验方案,实现对危险感知的预定分级;设计配对T检验和Wilcoxon符号秩检验结合的方式实现强弱感知状态下特征差异性的量化比较;建立基于十倍交叉参数调优SVM算法的危险感知状态二分类模型。研究结果表明:驾驶员在强危险感知状态下对危险的反应更主动,倾向于避免危险,而非紧急避险,并保持更低的车速,更倾向油门控制而非制动控制,注视和眼跳行为增加;驾驶员在隐性危险源场景中的操纵力度和频次更强,危险感知与显隐性影响程度及危险源类型有关,摩托车类危险源差异最大,行人类危险源差异最小;在c=1,γ=0.1,选择车头时距、车速标准差、最大制动踏板力、加速度标准差、预减速时间、车速均值、油门开度标准差、跳视次数、注视次数作为特征时,SVM模型具有最好的性能,准确率为89.2%,精准率为90.6%,召回率为87.8%,F1值为0.888。XGBoost模型对弱感知的识别能力低于SVM模型。该研究对于驾驶员危险感知状态的量化评估具有显著的指导意义。

关键词：交通工程感知机理机器学习危险感知强弱感知状态

尿毒清颗粒联合非布司他治疗高尿酸血症肾损害患者研究

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实用医学杂志 2024年第10期40卷 1418-1422页

作者：李娟杨林河北医科大学第二医院肾内科石家庄050000

目的探讨尿毒清颗粒联合非布司他治疗高尿酸血症(HUA)肾损害患者临床研究。方法选择河北医科大学第二医院肾内科于2020年3月至2022年3月HUA肾损害患者82例,运用随机数字表法分为观察组(n=41)与对照组(n=41)。对照组患者采用非布司他片治... 详细信息

目的探讨尿毒清颗粒联合非布司他治疗高尿酸血症(HUA)肾损害患者临床研究。方法选择河北医科大学第二医院肾内科于2020年3月至2022年3月HUA肾损害患者82例,运用随机数字表法分为观察组(n=41)与对照组(n=41)。对照组患者采用非布司他片治疗,观察组在非布司他片基础上联合尿毒清颗粒治疗。2组疗程12周。比较2组治疗疗效,治疗前后血尿酸、肾功能、cys c和β2-MG水平变化及不良反应情况。结果观察组HUA肾损害患者总有效率高于对照组(Pcr和24h Upro水平低于治疗前(Pcr和24h Upro水平低于对照组(Pcys c和β2-MG水平低于治疗前(Pcys c和β2-MG水平低于对照组(Pcys c和β2-MG水平,对患者肾功能具有保护作用。

关键词：尿毒清颗粒非布司他片高尿酸血症肾损害

Latest developments in early diagnosis and specific treatment of severe influenza infection

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Journal of Intensive Medicine 2024年第2期4卷 160-174页

作者： Francisco Valenzuela-Sánchez Blanca Valenzuela-Méndez juan Francisco Rodríguez-Gutiérrez Ángel Estella Intensive care Unit University Hospital of JerezRonda de Circunvalación s/nJerez de la FronteraSpain Haematology Department University Hospital of JerezRonda de Circunvalación s/nJerez de la FronteraSpain centro de Investigación Biomédica en Red Enfermedades respiratoriasCIBERESInstituto de Salud Carlos IIIAv.de Monforte de LemosMadridSpain Department of Oncological Surgery Institut du Cancer de Montpellier(ICM)Parc Euromédecine208 Av.des ApothicairesMontpellierFrance Department of Medicine Faculty of MedicineUniversity of CádizCalle Doctor MarañónCádizSpain Instituto de Investigación e Innovación Biomédica de cádiz(INIBicA) Avenida Ana de Viya 21CádizSpain

Influenza pandemics are unpredictable recurrent events with global health,economic,and social *** objective of this review is to provide an update on the latest developments in early diagnosis and specific treatment of the disease and its complications,particularly with regard to respiratory organ *** advances in treatment,the rate of mortality in the intensive care unit remains approximately 30%.Therefore,early identification of potentially severe viral pneumonia is extremely important to optimize treatment in these *** pathogenesis of influenza virus infection depends on viral virulence and host ***,in some patients,it is associated with an excessive systemic response mediated by an authentic cytokine *** process leads to severe primary pneumonia and acute respiratory distress *** prognostication in the emergency department based on comorbidities,vital signs,and biomarkers(e.g.,procalcitonin,ferritin,human leukocyte antigen-DR,mid-regional proadrenomedullin,and lactate)is *** of these biomarkers on admission may facilitate clinical decision-making to determine early admission to the hospital or the intensive care *** decisions are reached considering pathophysiological circumstances that are associated with a poor prognosis(e.g.,bacterial co-infection,hyperinflammation,immune paralysis,severe endothelial damage,organ dysfunction,and septic shock).Moreover,early implementation is important to increase treatment *** on a limited level of evidence,all current guidelines recommend using oseltamivir in this *** possibility of drug resistance should also be *** options include other antiviral drugs and combination therapies with monoclonal ***,it is not recommended to use corticosteroids in the initial treatment of these ***,the implementation of supportive measures for respiratory failure is ***

关键词： Influenza Pandemic Respiratory failure Acute respiratory distress syndrome Intensive care unit admission

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中国创新驱动水平测度、地区差异与动态演进

统计与决策 2024年第4期40卷 56-61页

作者：唐娟王华秦放鸣长沙民政职业技术学院商学院长沙417000 唐山师范学院海洋学院河北唐山063000 新疆大学经济与管理学院乌鲁木齐830046

创新驱动是高质量发展的第一动力,文章界定了创新驱动的内涵,明确其内在作用机制,在此基础上概括出认知基础、主体投入、成果产出和环境支撑四大维度。基于四大维度构建创新驱动水平评价指标体系,运用主成分分析法测算出创新驱动水平,... 详细信息

创新驱动是高质量发展的第一动力,文章界定了创新驱动的内涵,明确其内在作用机制,在此基础上概括出认知基础、主体投入、成果产出和环境支撑四大维度。基于四大维度构建创新驱动水平评价指标体系,运用主成分分析法测算出创新驱动水平,并借助Dagum基尼系数和Kernel密度估计方法揭示创新驱动水平的区域差异及演进趋势。结果表明:(1)虽然中国整体创新驱动水平大幅度提高,但是,地区之间的差异也越来越大,东部地区的创新驱动发展水平显著高于中西部地区;(2)各省份间的创新驱动水平差距较大,甚至部分省份的创新驱动水平在某些年份出现倒退的情况;(3)创新驱动的制度保障还不完善,地区之间的技术扩散和转移机制不畅,造成了不同的创新驱动发展路径。

关键词：创新驱动地区差异主成分分析 Kernel密度估计

盆底康复治疗患者的分布及变化趋势分析:一项基于3033例患者的研究

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实用妇产科杂志 2024年第8期40卷 639-644页

作者：吴纯华刘娟广州医科大学附属第三医院妇产科、广东省产科重大疾病重点实验室、广东省妇产疾病临床医学研究中心、粤港澳母胎医学高校联合实验室广东广州510150

目的:分析接受盆底康复治疗患者的分布及变化特征,探讨其有临床症状的相关影响因素、就诊特征。方法:对2010年1月至2019年12月广州医科大学附属第三医院接受盆底康复治疗患者的年龄、分娩方式、产次、治疗时间等数据进行回顾性分析。结... 详细信息

目的:分析接受盆底康复治疗患者的分布及变化特征,探讨其有临床症状的相关影响因素、就诊特征。方法:对2010年1月至2019年12月广州医科大学附属第三医院接受盆底康复治疗患者的年龄、分娩方式、产次、治疗时间等数据进行回顾性分析。结果:①接受盆底康复治疗患者共3033例,无临床症状者72.8%(2209/3033),近十年呈下降趋势(P=0.000);有临床症状者27.2%(824/3033),呈上升趋势(P=0.000),8类临床症状中尿失禁症状的比例为72.5%(597/824)。②十年间盆底康复治疗患者中,40岁以上患者比例逐年增加(P=0.001);③经阴道分娩的妇女所占比例最高(70.3%),近十年趋势保持平稳(P=0.054)。④十年间盆底康复治疗患者中初产妇占比最高(73.1%),经产妇(产次≥2)的患者有增高趋势(P=0.000);⑤84.8%(2573/3033)患者进行盆底康复治疗距离末次分娩时间<1年,治疗距离末次分娩时间≥1年的患者呈增多的趋势(P=0.002,r=0.855)。⑥年龄≥40岁(OR 10.884,P<0.001)、孕次为3次(OR 1.634,P=0.001)、孕次≥4次(OR 1.908,P<0.001)、产次为2次(OR 1.305,P=0.038)、产次≥3次(OR 2.327,P=0.001)、经阴道分娩(OR 2.079,P<0.001)是已生育接受盆底康复治疗患者有临床症状的独立危险因素。结论:接受盆底康复治疗有临床症状患者中,以尿失禁症状为主,呈增加的趋势;强化对年龄≥40岁、有阴道分娩史、孕次≥3次、产次≥2次的女性早期筛查和干预,对年龄≥40岁、距离末次分娩≥1年的女性可考虑将治疗时间前移。

关键词：女性盆底功能障碍性疾病盆底康复治疗临床特点

Identification of the cytochrome P450s responsible for the biosynthesis of two types of aporphine alkaloids and their de novo biosynthesis in yeast

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Journal of Integrative Plant Biology 2024年第8期66卷 1703-1717页

作者： Qishuang Li Xiang Jiao Xinyi Li Wenlong Shi Ying Ma Xiangmei Tan Jingyi Gan Jimei Liu Jian Yang Jian Wang Baolong Jin Tong chen Ping Su Yujun Zhao Yifeng Zhang Jinfu Tang Guanghong cui Yun chen juan Guo Luqi Huang School of Traditional chinese Pharmacy China Pharmaceutical UniversityNanjing 211198China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Department of Life Sciences Chalmers University of TechnologyGothenburg SE-41296Sweden State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaThe Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Aporphine alkaloids have diverse pharmacological activities;however,our understanding of their biosynthesis is relatively *** studies have classified aporphine alkaloids into two categories based on the configuration and number of substituents of the D-ring and have proposed preliminary biosynthetic pathways for each *** this study,we identified two specific cytochrome P450 enzymes(cYP80G6 and cYP80Q5)with distinct activities toward(S)-configured and(R)-configured substrates from the herbaceous perennial vine Stephania tetrandra,shedding light on the biosynthetic mechanisms and stereochemical features of these two aporphine alkaloid ***,we characterized two cYP719c enzymes(cYP719c3 and cYP719c4)that catalyzed the formation of the methylenedioxy bridge,an essential pharmacophoric group,on the A-and D-rings,respectively,of aporphine *** the functional characterization of these crucial cytochrome P450 enzymes,we reconstructed the biosynthetic pathways for the two types of aporphine alkaloids in budding yeast(Saccharomyces cerevisiae)for the de novo production of compounds such as(R)-glaziovine,(S)-glaziovine,and *** study provides key insight into the biosynthesis of aporphine alkaloids and lays a foundation for producing these valuable compounds through synthetic biology.

关键词： aporphine alkaloids biosynthesis cYP719c cYP80 engineered yeast

Study on Detection of Antibiotic contents in Water around Landfill Sites

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Agricultural Biotechnology 2024年第2期13卷 33-37页

作者： Sha FENG Dandan LIU juan LUO Qing LI Tao MO Zheng LIU Xiaonan ZOU Guangzhou GRG Metrology&Test(Hunan)co. Ltd.Changsha 410007China Anhua Administration for Market Regulation Yiyang 413500China Guizhou Engineering Vocational college Tongren 565200China

[Objectives] An ultra performance liquid chromatography-tandem mass spectrometry (UPLc-MS/MS) method was established for simultaneous determination of 26 antibiotics in the water around landfills. [Methods] After an HLB solid-phase extraction column was activated, and a water sample, which was adjusted with phosphoric acid to a pH of (2±0.5) and added with 500 mg of disodium EDTA, was loaded, and 5 ml of water and 20% methanol water was added for washing. Next, 10 ml of elution solution was added for elution, and the collected eluate was evaporated under reduced pressure at 40 ℃ to near dryness, and 1 ml of reconstitution solution was added to a constant volume. An AcQUITY UPLc BEH c18 (100 mm×2.1 mm, 2.6 μm) chromatographic column was adopted for Lc separation by gradient elution with 0.1% formic acid aqueous solution-acetonitrile as the mobile phase. For MS detection, the MRM mode was adopted for collection, and the positive and negative ion modes were switched for simultaneous determination, and the internal standard method was used for quantification. [Results] The correlation coefficient R2 was greater than 0.99 in the linear range of each target substance. The limits of detection ranged from 0.15 to 3.00 ng/L, and the limits of quantitation were between 0.80 and 10.00 ng/L, and the recoveries ranged from 77.9% to 104.85%. [conclusions] The method has high sensitivity, good accuracy and strong practical value.

关键词： Landfill Antibiotics Ultra high performance liquid chromatography-tandem mass spectrometry