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化瘀消癥复方靶向MAPK通路诱导滋养细胞自噬性死亡的机制研究

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时珍国医国药 2024年第9期35卷 2156-2160页

作者：段凯旋曹珊邓高丕柴淑娟严玉鸽耿斯羽聂兴源王焱皙河南中医药大学河南郑州450046 广州中医药大学第一附属医院广东广州510405 河南中医药大学第三附属医院河南郑州450000

目的基于MAPK通路,探讨化瘀消癥复方对输卵管妊娠滋养细胞自噬性死亡的调控机制。方法选用不同浓度的MAPK信号通路抑制剂SB202190干预滋养细胞,MTS法及免疫印迹法检测MAPK通路抑制对滋养细胞增殖、自噬的影响;联合化瘀消癥复方含药血清... 详细信息

目的基于MAPK通路,探讨化瘀消癥复方对输卵管妊娠滋养细胞自噬性死亡的调控机制。方法选用不同浓度的MAPK信号通路抑制剂SB202190干预滋养细胞,MTS法及免疫印迹法检测MAPK通路抑制对滋养细胞增殖、自噬的影响;联合化瘀消癥复方含药血清干预滋养细胞,MTS法检测细胞增殖活力,免疫印迹法检测自噬、凋亡、MAPK通路相关蛋白的表达,流式细胞术检测细胞凋亡情况。结果与0μmol/L SB202190比较,5、10、20μμmol/L的药效浓度均可抑制HTR-8/SVneo细胞增殖,并呈现浓度依赖性;5、10μμmol/L的SB202190可上调LC3-Ⅱ/LC3-Ⅰ的表达,下调P62的表达(P<0.05)。化瘀消癥复方含药血清联合SB202190干预后,可显著抑制HTR-8/SVneo细胞增殖;诱导凋亡(P<0.05)。免疫印迹结果显示,联合用药组LC3-Ⅱ/LC3-Ⅰ、Bax表达上调,P62、Bcl-2表达下降,p-MAPK、p-ERK蛋白表达下调(P<0.05)。结论化瘀消癥复方含药血清通过抑制MAPK通路,抑制HTR-8/Svneo细胞的活力,诱导妊娠滋养细胞自噬性死亡,发挥治疗异位妊娠的效果。

关键词：化瘀消癥复方含药血清 MAPK通路自噬性死亡异位妊娠

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中国油料作物能源利用效率与温室气体排放

中国生态农业学报（中英文） 2023年第12期31卷 1984-1996页

作者：陈丽刘娟王末李牧中国农业科学院农业信息研究所/农业农村部农业大数据重点实验室北京100081 天津商业大学公共管理学院天津300134

作物生产中有效利用能源是可持续农业的一个重要目标。中国作为油料生产和消费大国,在其生产过程中,减少过度无效能源消耗,优化能源利用结构,提高能源利用效率,对于农民节本增收、降低温室气体排放和环境影响具有重要意义。本文基于生... 详细信息

作物生产中有效利用能源是可持续农业的一个重要目标。中国作为油料生产和消费大国,在其生产过程中,减少过度无效能源消耗,优化能源利用结构,提高能源利用效率,对于农民节本增收、降低温室气体排放和环境影响具有重要意义。本文基于生命周期分析(LCA)视角,采用生命周期评价和数据包络分析(DEA)方法,对油菜、大豆、花生3种油料作物能源利用效率和温室气体排放进行了测算,分析了不同省份能源高效和低效利用的原因,探究了各省能源节约和温室气体减排的可能性与潜力。结果表明:1)3种油料作物生产系统单位能量消耗的产出能力差异不显著,但能源利用效率差异显著,呈花生>油菜>大豆的特点。2)3种油料作物中,花生温室气体排放量最高[874.96 kg(CO_(2) eq)∙hm^(-2)],其次为油菜[660.16 kg(CO_(2)eq)∙hm^(-2)],大豆最低[507.07 kg(CO_(2)eq)∙hm^(-2)];不同油料作物物质投入和农事操作温室气体排放贡献差异明显,油菜与花生种植主要温室气体排放源为化肥,而大豆种植过程中化肥、柴油、灌溉温室气体排放贡献相对均衡。3)油料作物能源利用优化和温室气体减排潜力较大,油菜、大豆、花生低效省份能源利用优化后,分别可节约11.97%、16.38%和15.89%的资源,可减排20.60~616.32kg(CO_(2)eq)∙hm^(-2)。因此,根据区域实际情况,优化能源低效利用地区的能源利用结构,探寻产量与碳排双优的生产模式,对推动油料作物种植节本增收与绿色发展将发挥重要作用。

关键词：油料作物能源利用效率温室气体排放生命周期评价模型数据包络分析模型

维普期刊数据库博看期刊

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基于信息熵理论的教育网异常流量发现

计算机应用研究 2010年第4期27卷 1434-1436页

作者：陈锶奇王娟电子科技大学计算机科学与工程学院成都611731

为提高异常流量发现的效率,解决传统流量分析方法效率较低、异常检测能力弱的问题,对骨干路由器的netflow流数据采用基于多个信息熵的联合指标并结合基于滑动窗口的熵流突发检测算法来实现网络异常的发现;并利用各指标熵值的相关度分析... 详细信息

为提高异常流量发现的效率,解决传统流量分析方法效率较低、异常检测能力弱的问题,对骨干路由器的netflow流数据采用基于多个信息熵的联合指标并结合基于滑动窗口的熵流突发检测算法来实现网络异常的发现;并利用各指标熵值的相关度分析将指标分类,根据已知的异常类型对每一类指标的异常检测范围作出总结。通过实验成功剔除了冗余度高的指标,将网络异常流量分为了能准确地被联合指标识别出的四种类型。实验证明,该异常检测方案实用性强,较传统的流量分析方法在异常类型的判断上更加准确和有效。

关键词：信息熵基于移动窗口的熵流突发检测算法 netflow 网络异常发现相关度分析

Changes of Reactive Oxygen Species and Related Enzymes in Mitochondria Respiratory Metabolism During the Ripening of Peach Fruit

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Agricultural Sciences in China 2010年第1期9卷 138-146页

作者： KAN juan,wang Hong-mei,JIN Chang-hai and XIE Hai-yan College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,P.R.China [a] College of Food Science and Engineering Yangzhou University Yangzhou 225127 P.R. China

The fruits of peach cultivar Yuhua 3 were used as materials to investigate the changes of active oxygen and related enzymes in mitochondria respiratory metabolism during ripening of peach fruit, involving their influence on the proceeding of peach fruit senescence. The results showed that the large decrease in firmness occurred between maturity II and IV. The decrease in firmness coincided with an increase in respiratory intensity. Obvious peaks of respiratory intensity lagging to the rapid change of fruit firmness could be shown during peach ripening. Reactive oxygen species （ROS） had a cumulative process and positively correlated with respiratory intensity. During peach ripening, the content of Ca^2＋ increased, the activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, H＋-ATPase, and Ca^2＋-ATPase decreased varying in different degree at the later step of ripening. These suggested a close relationship existed between ROS metabolism and mitochondrial respiration, namely, both ROS metabolism and mitochondrial respiration probably played important roles in ripening and senescing of peach fruit.

关键词： peach fruit ripening reactive oxygen species enzymes related to mitochondria respiratory metabolism

Carbon dot-modified silicon nanoparticles for lithium-ion batteries

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International Journal of Minerals,Metallurgy and Materials 2021年第10期28卷 1603-1610页

作者： Qiao-kun Du Qing-xia Wu Hong-xun wang Xiang-juan Meng Ze-kai Ji Shu Zhao Wei-wei Zhu Chuang Liu Min Ling cheng-du Liang Zhejiang Provincial Key Laboratory of Advanced Chemical Engineering Manufacture Technology College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China Zhejiang Research Institute of Chemical Industry Hangzhou 310000China Sinochem Lantian Corporation Hangzhou 310000China

Silicon(Si)particles were functionalized using carbon dots(CDs)to enhance the interaction between the Si particles and the ***,CDs rich in polar groups were synthesized using a simple hydrothermal ***,CDs were loaded on the Si surface by impregnation to obtain the functionalized Si particles(Si/CDs).The phases and microstructures of the Si/CDs were observed using Fourier-transform infrared reflection,X-ray diffraction,scanning electron microscopy,and high-resolution transmission electron ***/CDs were used as the active material of the anode for electrochemical performance *** electrochemical performance of the Si/CD electrode was assessed using cyclic voltammetry,electrochemical impedance spectroscopy,and constant current charge and discharge *** electrodes prepared with Si/CDs showed good mechanical structure stability and electrochemical *** 150 cycles at 0.2 C,the capacity retention rate of the Si/CD electrode was 64.0%,which is twice as much as that of pure Si electrode under the same test conditions.

关键词： binder silicon anodes lithium-ion batteries carbon dot multifunctional

Research on Comprehensive Benefits Evaluation of Prefabricated Buildings Energy Saving

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Research on Comprehensive Benefits Evaluation of Prefabricat...

Prediction of chemotherapeutic response in unresectable non-small cell lung cancer(NSCLC)patients by3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS)assay

作者： juan wang Shitong wang School of Economics and Management Jiangxi University of Science and Technology

In order to gain a deep understanding of the comprehensive benefits of prefabricated building energy *** the three aspects of economic benefit,environmental benefit and social benefit,the Delphi method was used to screen out 12 indicators.A comprehensive evaluation system for energy efficiency of fabricated buildings was constructed,and the comprehensive benefits of various energy saving measures were evaluated by TOPSIS entropy weight *** results show that the energy efficiency of energy system and equipment efficiency,passive design energy conservation and the balance of building energy consumption through the use of renewable energy systems and the replacement of energy-saving three energy-saving measures can promote the improvement of comprehensive *** benefits of energy efficiency and energy efficiency through energy systems and equipment are most ***,the scientific and effective evaluation of comprehensive benefits evaluation indicators and evaluation methods was verified by examples.

关键词： Prefabricated building Energy saving Comprehensive benefits Entropy weight decision

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Prediction of chemotherapeutic response in unresectable non-...

The eligibility criteria,training content,and scope of practice for prescriptive authority for midwives:a modified Delphi study

2014年广东省药师周大会

作者： juan chen Guo-Hua cheng Li-Pai chen Ting-Yuan Pang Xiao-Le wang Department of Pharmacy The Affiliated Cancer Hospital of Guangzhou Medical College College of Pharmacy Jinan University Department of Gynecology The Affiliated Cancer Hospital of Guangzhou Medical College

Background Selecting chemotherapy regimens guided by the chemosensitivity test can provide individualized therapies for cancer patients.(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,inner salt)(MTS) assay as one of in vitro chemosensitivity assays has become widely used to evaluate the sensitivity to anticancer agents of *** aim of this study was to evaluate the clinical applicability and accuracy of MTS assay for predicting chemotherapeutic response in the unresectable NSCLC *** cancer cells were isolated from malignant pleural effusion of unresectable non-small cell lung cancer(NSCLC) patients by density gradient centrifugation,and the sensitivity of them to eight chemotherapeutic agents was examined by MTS assay,and the relationship between in vitro chemosensitivity and the clinical response was also *** A total of 37 patients participated in this study,and MTS assay produced results successfully in 34 patients(91.9%).The sensitivity rates of 34 patients to chemotherapeutic agents ranged from 8.8%to 88.2%.Twenty-four of 34 patients who received chemotherapy were evaluated for in vitro-in vivo response *** correlation between in vitro chemosensitivity result and in vivo response was highly significant(P=0.003),and the total predictive accuracy,sensitivity,specificity,positive predictive value,and negative predictive value for MTS assay were 87.5%,94.1%,71.4%,88.9%,and83.3%,*** in vitro sensitivity for CDDP showed a significant correlation with in vivo response(P=0.018,r=0.522).Conclusion MTS assay is a preferable in vitro chemosensitivity assay that could be use to predict the response to chemotherapy and select the appropriate chemotherapy regimens for unresectable NSCLC patients,which could greatly improve therapeutic efficacy and reduce unnecessary adverse effects.

关键词： Chemosensitivity Chemotherapeutic agents MTS assay Non-small cell lung cancer

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Frontiers of Nursing 2019年第3期6卷 191-201页

作者： Shi-Fan Han Hong-Xia Ren Xiu-juan wang Xiao-Ding Yue Si-Yu Dou School of Nursing Shanxi Medical UniversityTaiyuanShanxi 030001China First Hospital of Shanxi Medical University TaiyuanShanxi 030001China

Objective:To investigate the necessary eligibility criteria for prescriptive authority for midwives,gather suggestions from experts on training content for prescriptive authority for midwives,and explore the scope of practice of their prescriptive authority in certain *** results of this study could serve as a reference for the development of policies on prescriptive authority for ***:Based on a literature search and semistructured interviews,a modified Delphi method was first used to conduct 2 rounds of expert consultation on eligibility criteria and training content for prescriptive authority for *** stage included nursing experts(20)and medical experts(16)engaged in midwifery in many tertiary Grade A hospitals in ***,consultation on the scope of practice of prescriptive authority for midwives was conducted with nursing experts(18)and medical experts(14)engaged in midwifery in many tertiary Grade A hospitals in *** suggestions from the experts were analyzed using statistical methods to confirm the eligibility criteria for prescriptive authority,training content,circumstances,scope of practice for prescriptive authority,and the prescription ***:Among the consulted experts,70.59%(the highest acceptance rate)considered an undergraduate degree to be the minimum educational requirement for midwives to be eligible for prescriptive authority,85.29%(the highest acceptance rate)considered the supervisor nurse to be the minimum technical position experience necessary for midwives to be eligible for having prescriptive authority,and 50%(the highest acceptance rate)considered 5 years to be the minimum number of years of experience in the specialty for midwives to be eligible for prescriptive *** applicants should at least be practicing at Grade C hospitals,which was the consensus among 91.18%of the consulted *** the consulted experts,100%,100%,97.06%,94.12%,and 94.12%agreed that the applic

关键词： Delphi study midwife eligibility criteria training content circumstances scope of practice prescriptive authority prescription forms

Optical absorption tunability and local electric field distribution of gold-dielectric-silver three-layered cylindrical nanotube

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Chinese Physics B 2021年第11期30卷 358-366页

作者： Ye-Wan Ma Zhao-wang Wu Yan-Yan Jiang juan Li Xun-Chang Yin Li-Hua Zhang Ming-Fang School of Mathematics and Physics Anqing Normal UniversityAnqing 246133China

The effects of inner nanowire radius,shell thickness,the dielectric functions of middle layer and surrounding medium on localized surface plasmon resonance(LSPR)of gold-dielectric-silver nanotube are studied based on the quasi-static *** calculation results show that LSPR of gold-dielectric-silver nanotube and LSPR numbers can be well optimized by adjusting its geometrical *** longer wavelength of |ω--> mode takes place a distinct red-shift with increasing the inner nanowire radius and the thickness of middle dielectric layer,while a blue-shift with increasing outer nanotube *** physical mechanisms are explained based on the plasmon hybridization theory,induced charges and phase *** addition,the effects of middle dielectric function and surrounding medium on LSPR,and the local electric field factor are also *** study provides the potential applications of gold-dielectricsilver nanotube in biological tissues,sensor and related regions.

关键词： localized surface plasmon resonance gold-dielectric-silver nanotube quasi-static approximation plasmon hybridization theory