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Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

Advances in Microbiology 2013年第8期3卷 24-30页

作者： mar.a de Guadalupe Moctezuma-zárate juan manuel Vargas-Morales juan F. Cárdenas-González Víctor manuel mar.ínez-Juárez Ismael Acosta-Rodríguez área Académica de Medicina Veterinaria y zootecnia Instituto de Ciencias Agropecuarias Universidad Autónoma del Estado de Hidalgo Tulancingo de Bravo Hidalgo México Laboratorio de Micología Experimental Centro de Investigación y de Estudios de Posgrado Facultad de Ciencias Químicas Universidad Autónoma de San Luis Potosí San Luis Potosí México

Fusarium solani is a necrotrophic parasitic fungus that causes wilt in some plants, causing severe economic losses in some areas of the country. The objective of this work was to analyze the induction of extracellular lytic enzymes produced by a strain of F. solani, isolated from a culture of tomato, in Villa de Arista, S.L.P. México. Polygalacturonase activity has a greater induction time at 10 days, and the xylanase has two times higher activity at 8 and 13 days of incubation at 28?C. Also, the xylanase activities A and B were very stable at 4?C. After 7 days of incubation, it has an activity of 100% and 96%, respectively, while polygalacturonase retains 61% of its initial activity. Both activities are better induced with glutamate and urea as nitrogen sources respectively, and both exhibit an initial pH optimum of 5.5. Finally, we didn’t find cellulase activity in the analyzing conditions.

关键词： Fusarium solani necrotrophic Fungus Lytic Enzymes

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Microcystis aeruginos strain[D-Leu^(1)]Mcyst-LR producer,from Buenos Aires province,Argentina

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Journal of Coastal Life Medicine 2014年第4期 287-296页

作者： Lorena Rosso Daniela Sedan mar.a Kolman Josep Caixach Cintia Flores juan manuel Oteiza Graciela Salerno Ricardo Echenique Leda Giannuzzi Darío Andrinolo Cátedra de Toxicología Facultad de Ciencias ExactasUniversidad Nacional de La Plata48 y 115(1900)La PlataArgentina CEBB-CIB Fundación para Investigaciones Biológicas Aplicadas(FIBA-mar.del Plata) Mar del PlataArgentina Laboratori d'Espectrometria de Masses/Contaminants Orgànics Institut de Diagnosi Ambiental i Estudis de l'Aigua(IDAEA)CSIC.J.Girona18-2608034-BarcelonaSpain División Ficología Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataPaseo del Bosque s/n°(1900)La PlataArgentina

Objective:To show the toxicological and phylogenetic characterization of a native Microcystis ceruginosa(***)strain(named CAAT 2005-3)isolated from a water body of Buenos Aires province,***:A *** strain was isolated from the drainage canal of the sewage treatment in the town of Pila,Buenos Aires province,Argentina and acclimated to laboratory *** amplification of cpcBA-IGS Phcocyanin(PC,intergenic spacer and flanking regions)was carried out in order to build a phylogenetic *** exactive/orbitrap mass spectrometer equipped with an electrospray ionization source(Thermo Fisher Scientific,Bremen,Germany)was used for the LC/ESI-HRMS microcystins *** number of cell/mL and[D-Leu^(1)]Mcyst-LR production obtained as a function of time was modelled using the Gompertz ***:The phylogenetic analysis showed that the sequence clustered with others *** sequences obtained from *** first Argentinian strain of ***(CAAT 2005-3)growing under culture conditions maintains the typical colonial architecture of *** with profuse *** CAAT 2005-3 expresses a toxin variant,that was identified by LC-HRMs/Orbitrapas as[D-Leu^(1)]microcystin-LR([M+H]+=1037.8 m/z).Conclusions:[D-Leu^(1)]microcystin-LR has been also detected in *** samples from Canada,Brazil and *** work provides the basis for technological development and production of analytical standards of toxins present in our region.

关键词： Cyanobacteria Microcystis aeruginosa [D-Leu^(1)]microcystin-LR[M+H]+ Harmful algal blooms

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