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Photosynthetic and Molecular Markers of CO_2-mediated Photosynthetic Downregulation in Nodulated Alfalfa

Journal of Integrative Plant Biology 2013年第8期55卷 721-734页

作者： Alvaro Sanz-Sez Gorka Erice Iker Aranjuelo Ricardo Aroca juan Manuel Ruíz-Lozano Jone Aguirreolea juan jos irigoyen Manuel Sanchez-Diaz Department of Plant Biology University of NavarraPamplonaE-31008Spain Department of Plant Biology University of BarcelonaBarcelonaE-8028Spain Department of Plant Biology University of Illinois at Urbana-Champaign61801USA Agrobiotechnology Institute Public University of NavarraMutilva BajaE-31192Spain Department of Soil Microbiology and Symbiotic Systems Zaidin Experimental Station(CSIC)GranadaE-18008.Spain

Elevated CO2 leads to a decrease in potential net photosynthesis in long-term experiments and thus to a reduction in potential growth. This process is known as photosynthetic downregulation. There is no agreement on the definition of which parameters are the most sensitive for detecting CO2 acclimation. In order to investigate the most sensitive photosynthetic and molecular markers of CO2 acclimation, the effects of elevated CO2, and associated elevated temperature were analyzed in alfalfa plants inoculated with different Sinorhizobium meliloti strains. Plants （Medicago sativa L. cv. Aragon） were grown in summer or autumn in temperature gradient greenhouses （TGG）. At the end of the experiment, all plants showed acclimation in both seasons, especially under elevated summer temperatures. This was probably due to the lower nitrogen （N） availability caused by decreased N2-fixation under higher temperatures. Photosynthesis measured at growth CO2 concentration, rubisco in vitro activity and maximum rate of carboxylation were the most sensitive parameters for detecting downregulation. Severe acclimation was also related with decreases in leaf nitrogen content associated with declines in rubisco content （large and small subunits） and activity that resulted in a drop in photosynthesis. Despite the sensitivity of rubisco content as a marker of acclimation, it was not coordinated with gene expression, possibly due to a lag between gene transcription and protein translation.

关键词： Carbon dioxide Medicago sativa (alfalfa) photosynthetic downregulation RbcL and RbcS Sinorhizobium meliloti.

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Presynaptic endoplasmic reticulum architecture and hereditary spastic paraplegia

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Neural Regeneration Research 2024年第3期19卷 485-486页

作者： juan joséPérez-Moreno Instituto de Biomedicina de Sevilla(IBiS) Hospital Universitario Virgen Del Rocío/CSIC/Universidad de Sevillaand Departamento de Biología CelularFacultad de BiologíaUniversidad de SevillaSevilleSpain

Hereditary spastic paraplegia(HSP)is a clinically and genetically heterogeneous neurodegenerative disorder,characterized primarily by progressive spasticity and weakness in the lower *** ients can also experience peripheral neuropathy,cognitive impairment,and other neurological *** date,more than 80 genes have been implicated in HSP,encompassing various cellular components,although mutations in genes encoding endoplasmic reticulum(ER)-shaping proteins are the most prevalent(Parodi et al.,2017).ER-shaping proteins are generally known for regulating the tubulation and curvation of the ER,but most of them show additional functions,including fusion of ER tubules,microtubule-severing,ER autophagy,lipid droplet synthesis,contact sites with other organelles(Öztürk et al.,2020).This highlights the complexity of studying the role of these proteins and the link between ER function and HSP.

关键词： reticulum endoplasmic

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Conductometric Titration for the Prediction of Conductivity in Solutions with Polymeric Membranes

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Journal of Electronic Science and Technology 2017年第4期15卷 342-350页

作者： María josé Lavorante juan Isidro Franco Research and Development of Renewable Energy Department at CITEDEF Institute of Scientific and Technical Research for the Defense Villa Martelli B1603ALO Argentina

The analytical technique of conductometric titration is used to characterize polymeric *** technique allows obtaining the polymer dry weight capacity(DWC) and the extent of reaction and establishing the optimal number of water molecules per sulfonic *** this particular case the polymer material under study was Nafion?*** different reactions were made: Neutralization and *** of them allow obtaining the same *** titrations could be considered as a good method to study the neutralization and exchange reactions for polymeric materials used in fuel cells or *** implementation of this analytical technique allows reducing operation time of those types of *** waiting time between determinations permits to say that the amount of reactant consumed and products obtained were the same after each addition of titrant.

关键词： Conductivity conductometric titration polymeric membranes

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Evaluating the influence of air pollution on solar radiation observations over the coastal region of Alicante(Southeastern Spain)

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Journal of Environmental Sciences 2023年第4期126卷 633-643页

作者： Igor Gómez Sergio Molina juan joséGaliana-Merino Department of Applied Physics Faculty of SciencesUniversity of AlicanteSection 99E-03080 AlicanteSpain Multidisciplinary Institute for Environmental Studies(MIES)“Ramón Margalef” University of AlicanteSection 99E-03690 AlicanteSpain Department of Physics Systems Engineering and Signal TheoryUniversity of AlicanteSection 99E-03080 AlicanteSpain University Institute of Physics Applied to Sciences and Technologies University of AlicanteSection 99E-03080 AlicanteSpain

The present study evaluates ground-based downward surface shortwave radiation (Rs) over the coastal region of Alicante (Southeastern Spain).Hourly measurements collected over the eleven-year period 2010-2020 are *** weather stations located over the region capital,Alicante,have been selected as representative of urban and suburban *** additional weather stations far from the city have been selected representing rural *** is significantly reduced over the urban station during the morning hours within the winter season compared to the observations recorded over the suburban and rural stations,with a global mean difference of-81 and-120 W/m^(2)at 10 LT,***,no significant differences are obtained during the midday sun,with a global mean difference of-20 W/m^(2)between the urban and rural *** the aim of explaining these differences,the current paper investigates the relationship between Rsand different air pollutants:NOx,SO_(2),and fine particulate matter (PM_(2.5)and PM_(10)) as well as the wind field measured at the urban and suburban *** results found in this work point towards a close relationship between Rsand NOxconcentrations annual cycles,which are also influenced by the prevailing wind circulations observed over the study area.A global mean NOxconcentration of 107μg/m^(3)is observed over the urban station at 10 LT during the winter *** contrast,these high concentrations are significantly reduced over the suburban station,with global mean value of 40μg/m^(3)at 10 LT,for this period of the year.

关键词： Solar radiation Air pollution Weather-concentration relationship Coastal cities Atmospheric conditions Wind field

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The Impact of Hurricane Strikes on Short-Term Local Economic Activity: Evidence from Nightlight Images in the Dominican Republic

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International Journal of Disaster Risk Science 2019年第3期10卷 362-370页

作者： Oscar A.Ishizawa juan joséMiranda Eric Strobl World Bank Group SocialUrbanRural and Resilience(SURR)Global PracticeWashingtonDC20433USA Economic Research World Bank GroupWashingtonDC20433USA University of Bern 3012BernSwitzerland

The Dominican Republic is highly exposed to adverse natural events that put the country at risk of losing hard-won economic, social, and environmental gains due to the impacts of disasters. This study used monthly nightlight composites in conjunction with a wind field model to econometrically estimate the impact of tropical cyclones on local economic activity in the Dominican Republic since 1992. It was found that the negative impact of storms lasts up to 15 months after a strike, with the largest effect observed after 9 months. Translating the reduction in nightlight intensity into monetary losses by relating it to quarterly gross domestic product(GDP) suggests that on average the storms reduced GDP by about USD 1.1 billion(4.5% of GDP 2000 and 1.5% of GDP 2016).

关键词： Dominican Republic Econometric analysis Hurricanes Nightlights

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GenoDrawing:An Autoencoder Framework for Image Prediction from SNP Markers

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Plant Phenomics 2023年第4期5卷 816-824页

作者： Federico Jurado-Ruiz David Rousseau juan A.Botía Maria josé Aranzana Center for Research in Agricultural Genomics(CRAG) 08193 BarcelonaCerdanyolaSpain Universitéd'Angers LARISINRAe UMR IRHS49000 AngersFrance Department of Information and Communication Engineering University of Murcia30071 MurciaSpain IRTA(Institut de Recerca i Tecnologia Agroalimentàries) BarcelonaSpain

Advancements in genome sequencing have facilitated whole-genome characterization of numerous plant species,providing an abundance of genotypic data for genomic *** selection and neural networks(NNs),particularly deep learning,have been developed to predict complex traits from dense genotypic ***,an NN model to extract features from images in an unsupervised manner,has proven to be useful for plant *** study introduces an autoencoder framework,GenoDrawing,for predicting and retrieving apple images from a low-depth single-nucleotide polymorphism(SNP)array,potentially useful in predicting traits that are difficult to *** demonstrates proficiency in its task using a small dataset of shape-related *** indicate that the use of SNPs associated with visual traits has substantial impact on the generated images,consistent with biological *** using substantial SNPs is crucial,incorporating additional,unrelated SNPs results in performance degradation for simple NN architectures that cannot easily identify the most important *** proposed GenoDrawing method is a practical framework for exploring genomic prediction in fruit tree phenotyping,particularly beneficial for small to medium breeding companies to predict economically substantial heritable *** GenoDrawing has limitations,it sets the groundwork for future research in image prediction from genomic *** studies should focus on using stronger models for image reproduction,SNP information extraction,and dataset balance in terms of phenotypes for more precise outcomes.

关键词： Auto Drawing prediction

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Mushroom Production as an Alternative for Rural Development in a Forested Mountainous Area

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Journal of Mountain Science 2014年第2期11卷 535-543页

作者： josé *** josé R.GONZáLEZ-OLABARRIA juan MARTíNEZ DE ARAGóN Departament de Producció Vegetal i Ciència Forestal Universitat de Lleida-Agrotecnio Center (UdL-Agrotecnio).Av. Rovira Roure 191 E-25198 Lleida Spain Centre Tecnològic Forestal de Catalunya (CTFC-CEMFOR). Ctra. de St. Lloren? de Morunys km 2 E-25280 Solsona Spain

Wild mushrooms are recognized as important non-wood forest products in mountainous ecosystems, but their real potential for generating rural economies has not been fully evaluated due to the difficulties in obtaining reliable productivity data, minimizing their true potential as contributor to rural economies. Mushroom yield models based on large data series from Pinus forest ecosystems in the region of Catalonia(Spain), combined with data from the Spanish National Forest Inventory allow us to estimate the potential mushroom productivity by forest ecosystems. The results of 24,500 tons/yr of mushrooms of which 16,300 tons are classified as edible and 7,900 tons are commonly marketed demonstrate the importance of mushroom productions in Catalonian pine forests, mostly located in mountainous areas where the development of agricultural activities is limited. Economic mushroom value is estimated at 48 million € for the edible mushroom and 32 million € for those corresponding to marketable yields, confirming the potential of this non-wood forest product. These production results and corresponding economic values provide a basis for the incorporation of wild mushrooms as significant non-wood forest products in the development of forest policies in mountainous areas.

关键词： Non-wood forest products Mushroom models National Forest Inventory Economic value Mushroom potential estimation

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Multiparameter resonant imaging for studying cell interactions

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Light(Science & Applications) 2018年第1期7卷 652-653页

作者： joséjuan-Colás Thomas F Krauss Department of Physics The University of YorkHeslignton YO105DDUK Department of Electronic Engineering The University of YorkHeslignton YO105DDUK

The development of optical microscopy techniques has provided major insights into the machinery of life,from molecules to cells to tissues and *** techniques have been particularly successful in this respect by elucidating biological pathways and helping us understand the complex relationships that underpin all living *** example,the first use of fluorescence as an analytical tool in 18641 paved the way for the study of biological entities and interactions,but it was not until nearly 60 years later,in the 1930s,that fluorophores were first employed to perform biological investigations into specific tissue components and bacteria that do not *** advances led to the first labeling of an antibody with a visible label in 19413,which gave birth to the field of immunofluorescence and allowed us obtain unprecedented insight into antibody structure.

关键词： organs insight visible

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Developing spatially and thematically detailed backdated maps for land cover studies

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International Journal of Digital Earth 2017年第2期10卷 175-206页

作者： juan joséVidal-Macua Alaitz Zabala Miquel Ninyerola Xavier Pons Dep Geografia Grumets Research GroupUniversitat Autònoma de BarcelonaBellaterraCataloniaSpain Dep Biologia Animal Vegetal i EcologiaGrumets Research GroupUniversitat Autònoma de BarcelonaBellaterraCataloniaSpain

Global or regional land cover change on a decadal time scale can be studied at a high level of detail using the availability of remote sensing data such as that provided by ***,there are three main technical challenges in this ***,the generation of land cover maps without reference data is problematic(backdating).Second,it is important to maintain high accuracies in land cover change map products,requiring a reasonably rich legend within each ***,a high level of automation is necessary to aid the management of large volumes of *** paper describes a robust methodology for processing time series of satellite data over large spatial *** methodology includes a retrospective analysis used for the generation of training and test data for historical periods lacking reference *** methodology was developed in the context of research on global change in the Iberian *** this study we selected two scenes covering geographic regions that are representative of the Iberian *** each scene,we present the results of two classifications(1985-1989 and 2000-2004 quinquennia),each with a legend of 13 *** overall accuracy of over 92%was obtained for all 4 maps.

关键词： Land cover mapping Landsat statistical classifiers backdating Iberian Peninsula

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Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry

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Horticulture Research 2015年第1期2卷 293-300页

作者： joséjuan Ordaz-Ortiz Sofia Foukaraki Leon Alexander Terry Plant Science Laboratory Cranfield UniversityBedfordshireMK430ALUK

Plant hormones are important molecules which at low concentration can regulate various physiological *** spectrometry has become a powerful technique for the quantification of multiple classes of plant hormones because of its high sensitivity and *** developed a new ultrahigh pressure liquid chromatography–full-scan high-definition accurate mass spectrometry method,for simultaneous determination of abscisic acid and four metabolites phaseic acid,dihydrophaseic acid,7′-hydroxy-abscisic acid and abscisic acid glucose ester,cytokinins zeatin,zeatin riboside,gibberellins(GA1,GA3,GA4 and GA7)and indole-3-acetyl-L-aspartic *** measured the amount of plant hormones in the flesh and skin of two processing potato *** and Russet Burbank stored for up to 30 weeks at 6°C under ambient air ***,we report for the first time that abscisic acid glucose ester seems to accumulate in the skin of potato tubers throughout storage *** method achieved a lowest limit of detection of 0.22 ng g−1 of dry weight and a limit of quantification of 0.74 ng g−1 dry weight(zeatin riboside),and was able to recover,detect and quantify a total of 12 plant hormones spiked on flesh and skin of potato *** addition,the mass accuracy for all compounds(<5 ppm)was evaluated.

关键词： conditions. limit mass

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