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Evaluation of Morphological Changes of <i>Aeromonas caviae</i>Sch3 Biofilm Formation under Optimal Conditions

Advances in Microbiology 2012年第4期2卷 552-560页

作者： Erika Beatriz Angeles-Morales Ricardo Mondragón-Flores juan Pedro Luna-Arias Cinthia Teresa Enríquez-nieto Berenice Parra-Ortega Graciela Castro-Escarpulli Centro de Diagnóstico y Vigilancia Epidemiológica del Distrito Federal. Instituto de Ciencia y Tecnología del Distrito Federal Mexico City Mexico Departamento de Biología Celular Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) Mexico City Mexico Departamento de Bioquímica Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN) Mexico City Mexico Laboratorio de Bacteriología Departamento de Microbiología Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional (ENCB-IPN) Prolongación de Carpio y Plan de Ayala s/n Mexico City Mexico

Aeromonas is a Gram-negative bacterium that lives in aquatic habitats. It can be infective in humans. One of its remarkable attributes is the ability of biofilm formation. Many factors are involved in the construction of biofilms as has been described for Pseudomonas, Klebsiella, and Vibrio, among others. The aim of this work was to study the bacterial morphology during the establishment of biofilm through scanning electron microscopy (SEM) and transmission electron microscopy (TEM) with a modified microtiter plate assay and to determine the best conditions for the establishment of Aeromonas caviae Sch3 biolfilm in vitro. We observed several phenotypic changes, including surface appearance, size, presence of extracellular vesicles from 100 to 250 nm in diameter, and flagella. The best conditions for biofilm formation were to grow cultures at 28℃ at pH 6, as determined by the crystal violet assay. This is, to the best of our knowledge, the first study that describes the cell’s biological events involved in the establishment of biofilm formation of Aeromonas caviae Sch3 in vitro.

关键词： Biofilm Aeromonas Caviae Scanning Electron Microscopy Transmission Electron Microscopy Physical and Chemical Factors

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Inflammatory pseudotumour of the spleen associated with splenic tuberculosis

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World j.urnal of Gastrointestinal Surgery 2014年第12期6卷 248-252页

作者： Maria Isabel Prieto-nieto juan Pedro Pérez-Robledo Beatriz Díaz-San Andrés Manuel Nistal j.sé Antonio Rodríguez-Montes Department of General and Digestive Surgery La Paz University Hospital Paseo de la Castellana 28046 Madrid Spain Department of Pathology La Paz University Hospital Paseo de la Castellana 28046 Madrid Spain

Inflammatory pseudotumor(IPT) of the spleen is an uncommon entity with an uncertain aetiology. Inflammatory pseudotumors present diagnostic difficulties because the clinical and radiological findings tend to suggest a malignancy. The symptoms include weight loss, fever, and abdominal pain. Most cases of splenic IPT present solitary relatively large well circumscribed masses on imaging. The diagnosis in the maj.rity of the cases is made after histopathologic study of splenectomy specimens. The IPTs that occur in the spleen and liver are typically associated with Epstein-Barr virus. Thirtyseven percent of all new cases of active tuberculosis infection are extrapulmonary tuberculosis and tuberculous lymphadenitis the most commonly occurring form of extrapulmonary tuberculosis. We report the case of an inflammatory pseudotumor of the spleen associated with splenic tuberculous lymphadenitis in a 50-year-old female patient who was preoperatively diagnosed with a malignant spleen tumour based on her history of breast of carcinoma.

关键词： Inflammatory pseudotumour Spleen Splenic lymphadenitis Splenectomy Splenic tuberculosis

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Genomic Organization of Purinergic P2X Receptors

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Pharmacology & Pharmacy 2015年第8期6卷 341-362页

作者： Raúl Loera-Valencia j.sué Obed j.ramillo-Polanco Andrómeda Linan-Rico María Guadalupe nieto Pescador juan Francisco j.ménez Bremont Carlos Baraj.s-López División de Biología Molecular Instituto Potosino de Investigación Científica y Tecnológica San Luís Potosí México

Purinergic P2X receptors are a family of ligand-gated cationic channels activated by extracellular ATP. P2X subunit protein sequences are highly conserved between vertebrate species. However, they can generate a great diversity of coding splicing variants to fulfill several roles in mammalian physiology. Despite intensive research in P2X expression in both central and peripheral nervous system, there is little information about their homology, genomic structure and other key features that can help to develop selective drugs or regulatory strategies of pharmacological value which are lacking today. In order to obtain clues on mammalian P2X diversity, we have performed a bioinformatics analysis of the coding regions and introns of the seven P2X subunits present in human, simian, dog, mouse, rat and zebrafish. Here we report the arrangements of exon and intron sequences, considering its number, size, phase and placement;proposing some ideas about the gain and loss of exons and retention of introns. Taken together, these evidences show traits that can be used to gain insight into the evolutionary history of vertebrate P2X receptors and better understand the diversity of subunits coding the purinergic signaling in mammals.

关键词： Alternative Splicing Intron Genomic Organization P2X Purinergic Signalling

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一类具有变消耗率的恒化器模型的脉冲扰动与分支

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应用数学和力学 2009年第7期30卷 873-882页

作者：张弘 P.齐奥塞斯库 j.j.涅托陈兰荪江苏大学数学系江苏镇江212013 于默奥大学数学与数学统计系雅西"戈奥尔基-阿萨季"技术学院数学系圣地亚哥德孔波斯特拉大学数学分析系中国科学院数学研究所北京100080

提出和研究了一个具有变消耗率和非同步脉冲的恒化器模型,并且得到了一组像阈值一样的条件来确保系统半平凡周期解的全局渐稳性,系统的持久性以及出现非平凡分支周期解.最后,一些数值模拟体现了该模型的动力学性态.

关键词：恒化器脉冲微分方程持久性灭绝不动点方法分支

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<i>Stenotrophomonas maltophilia</i>Keratitis Related to Therapeutic Contact Lens Misidentified with an Automated Identification System

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Open j.urnal of Clinical Diagnostics 2015年第2期5卷 63-67页

作者： Daniel Fatela-Cantillo j.vier Crespo-González Blanca Fatela-Cantillo Antonio Fernandez-Suarez j.se Miguel Aguilar Benítez juan Antonio Saez nieto Remedios González-Amezcua Department of Biotechnology Hospital Alto Guadalquivir Andújar (Jaén) Spain Department of Ophthalmology Hospital Linares Linares (Jaén) Spain Department of Ophthalmology Hospital La Princesa Madrid (Madrid) Spain Department of Biotechnology Hospital Alcalá La Real Alcalá La Real (Jaén) Spain NationalMicrobiology Centre Majadahonda (Madrid) Spain Department of Ophthalmology Hospital Alto Guadalquivir Andújar (Jaén) Spain

We present a case of keratitis caused by Stenotrophomonas maltophilia in a therapeutic contact lens user with trichiasis and symblepharon. This keratitis was initially diagnosed as caused by Achromobacter xylosoxidans, but the strain was sent for species confirmation and the isolate was finally identified as S. maltophilia by means of 16S rDNA sequencing. The patient rapidly improved on administration of fortified ceftazidime. Physicians should be aware that the definitive identification of the pathogenic agent and prolonged antimicrobial treatment according to culture sensitivities in keratitis are mandatory as treatment success depends greatly on them.

关键词： Stenotrophomonas maltophilia Keratitis Ceftazidime

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Cesarean Scar Pregnancy with Iniencephaly and Progression to Placenta Accreta Due to Early Management Rej.ction

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Maternal-Fetal Medicine 2020年第1期2卷 56-58页

作者： Albaro j.sé nieto-Calvache María Camila López-Girón j.ime López-Tenorio juan Carlos Quintero-Mej.a María Andrea Zambrano-Regalado Harry Pachaj.a-Londoño María Fernanda Escobar-Vidarte Abnormally Invasive Placenta Clinic Fundación Valle del LiliCali 760032Colombia Clinical postgraduate department Universidad IcesiCali 760032Colombia Clinical Research Center Fundación Valle del LiliCali 760032Colombia Department of Ginecology and Obstetrics Centro médico ImbanacoCali 760032Colombia Department of Genetics Fundación Valle del LiliCali 760032Colombia

To editor:Cesarean scar pregnancy(CSP)is a rare pathology,with an increasingly clear association with morbidly adherent placenta(MAP).1 Although the most recommended treatment is pregnancy termination by cesarean section and scar resection shortly after diagnosis.2 The final decision regarding management depends on the patient's choice,medical advice,the fertility desire,and the social,religious and emotional background that should also be taken into account.

关键词： Placenta accreta Cesarean scar pregnancy Morbidity adherent placenta Pregnancy termination Ultrasonography Prenatal

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Clinicopathological differences,risk factors and prognostic scores for western patients with intestinal and diffuse-type gastric cancer

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World j.urnal of Gastrointestinal Oncology 2022年第6期14卷 1162-1174页

作者： Cristina Díaz del Arco Lourdes Estrada Muñoz Luis Ortega Medina Elena Molina Roldán MÁngeles Cerón nieto Soledad García Gómez de las Heras M j.sús Fernández Aceñero Department of Surgical Pathology Hospital Clínico San CarlosMadrid 28040Spain Department of Legal Medicine Psychiatry and PathologyComplutense University of MadridMadrid 28040Spain Department of Surgical Pathology Hospital Rey Juan CarlosMadrid 28933Spain Department of Basic Medical Sciences Rey Juan Carlos UniversityMadrid 28933Spain

BACKGROUND In the molecular era,the Laurén system is still a cost-effective and widely implemented classification for gastric cancer(GC)and it has been recently associated with clinical,histological and molecular features of these *** recent advances in the understanding of the molecular biology of GC,there is a need to develop new prognostic tools for patient stratification in clinical ***,the identification of easily available prognostic factors in patients with intestinal and diffuse-type tumors can significantly improve risk assessment and patient stratification in *** To identify clinicopathological differences,risk factors,and to develop costeffective prognostic scores for patients with intestinal and diffuse-type *** Retrospective study of all patients undergoing surgery for GC at a tertiary referral center from 2001 to 2019.286 cases met inclusion criteria(intestinal:190,diffuse:96).Clinical data and gross findings were *** specimens were reviewed by two independent pathologists and a detailed protocol for histologic evaluation was *** tissue microarrays(TMAs)were constructed and sections of the TMA block were immunostained for HERCEPTEST,MSH2,MSH6,MLH1 and *** analyses were performed and prognostic scores were developed based on hazard *** Intestinal and diffuse-type GC showed different epidemiological,clinicopathological and prognostic *** tumors were significantly associated with younger age,less symptomatology,flat morphology,deeper invasion,perineural infiltration,advanced stage at diagnosis,administration of adj.vant therapy and poorer *** lesions were fungoid or polypoid,showed necrosis,desmoplasia,microsatellite instability and HERCEPTEST positivity and were diagnosed at earlier *** depth,desmoplasia,macroscopic type and lymph node involvement were independently related to the Laurén ***,intestinal and diffuse GC were asso

关键词： Gastric cancer Clinicopathological Score Prognosis Laurén Molecular

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The GSTP1 gene variant rs1695 is not associated with an increased risk of multiple sclerosis

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Cellular & Molecular Immunology 2015年第6期12卷 777-779页

作者： j.se AG Agundez Elena Garcia-Martin Carmen Martinez j.lian Benito-Leon j.lian Benito-Leon j.rge Millan-PascualMaria Diaz-Sanchez Patricia Callej. Diana Pisa Laura Turpin-Fenoll Hortensia Alonso-Navarro Lucia Ayuso-Peralta Dolores Torrecillas Esteban Garcla-Albea j.se Francisco Plaza-nieto Felix j.vier j.menez-j.menez Department of Pharmacology University of Extremadura CAceres Spain Department of Biochemistry and Molecular Biology University of Extremadura Caceres Spain CiBERNED Centro de InvestigaciOn BiomEdica en Red de Enfermedades Neurodegenerativas Instituto de Salud Carlos III Spain Service of Neurology Hospital Universitario Doce de Octubre Madrid Spain Department of Medicine University Complutense Madrid Spain Section of Neurology Hospital La Mancha-Centro Alcazar de San Juan (Ciudad Real) Spain Centro de Biologfa Molecular Severo Ochoa (CSIC-UAM) Facultad de Ciencias Universidad Autonoma Cantoblanco 28049 Madrid Spain Department of Medicine-Neurology Hospital 'Principe de Asturias' Universidad de Alcala Alcala de Henares (Madrid) Spain Section of Neurology Hospital Universitario del Sureste Arganda del Rey (Madrid) Spain Correspondence: Dr FJ Jimenez-Jimenez C/Marroquina 14 3° B E-28030 Madrid Spain.

We analyzed the allelic and genotypic frequencies of the glutathione-S-transferase P1 （GSTPI） rs1695 single nucleotide polymorphism （SNP） in 290 patients with multiple sclerosis （MS） and in 310 healthy controls. We found no significant association between the rs1695 variant and MS. Among MS patients, there was no relationship between the rs1695 variant and either gender, clinical type of MS or the age of onset of MS.

关键词： associated increased sclerosis

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Physical and chemical characterization of sediments from an Andean river exposed to mining and agricultural activities: The Moquegua River, Peru

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International j.urnal of Sediment Research 2022年第6期37卷 780-793页

作者： Luis de Los Santos Valladares juan Luis Ccamapaza Renato Arturo Valencia-Bedregal Luis Enrique Borj.-Castro j.se Velazquez-Garcia Dehiwalage Harshani Nimalika Perera Adrian Ionescu David Arvidsson Eustace Peregrine Barnes Peter Newton Hugo Lepage Patrick Byrne Angel Guillermo Bustamante Dominguez Crispin H.W.Barnes Cavendish Laboratory Department of Physics University of Cambridge Escuela Acad emico Profesional de Ingeniería Ambiental Universidad Nacional de Moquegua Provincia de Mariscal Nieto Laboratorio de Cer amicos y Nanomateriales Facultad de Ciencias Físicas Universidad Nacional Mayor de San Marcos School of Biological and Environmental Science Liverpool John Moores University

This study presents an analysis of sediments in an Andean river impacted by both natural conditions and anthropogenic activities. Fifty samples were collected from selected sites throughout the Moquegua River drainage basin, and Tambo River headwaters at Pasto Grande, in Peru, and analysed with X-ray diffraction, energy dispersive X-ray spectroscopy, scanning electron microscopy, Fourier-transform infrared spectroscopy and thermogravimetric analysis. Si, Ca, Al, Fe, and O, common constituents of soils, were dominant, along with K, N, and P. The latter originating as primary macronutrients or chemical residuals from fertilizers used in agriculture. Higher concentrations of macronutrients and organic components were found in agricultural and urban areas, respectively. Arsenic minerals were also detected, which occur naturally, but possibly at levels augmented by anthropogenic activity. The application of cluster analysis revealed clustering between arsenic, arsenolite, and potassium but no significant geospatial correlation between sample sites in the drainage basin.

关键词： Andean river Moquegua river Sediments Fourier-transform infrared spectroscopy TG-DTG/DTA Clustering analysis

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Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez j.ana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Truj.llo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes j.ménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-j.remias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos j.séMiguel Cisneros Sonsoles Salto-Alej.ndre j.dith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma juan Carlos Crespo-Rivas Eduardo Márquez j.séManuel Lomas Claudio Bueno Rosario Amaya j.séAntonio Lepe j.rónimo Pachón Elisa Cordero j.vier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo j.vier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón j.sús Carbaj.l-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo juan Delgado Antonio Domínguez-Petit Alej.ndro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis j.ra Carlos j.ménez-juan Silvia j.ménez-j.rge Mercedes j.ménez-Sánchez j.lia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén j.séMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain Department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain Department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease *** this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 ***,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 Dendritic cell Long-COVID

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