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Recombinant streptokinase vs hydrocortisone suppositories in acute hemorrhoids:A randomized controlled trial

World Journal of Gastroenterology 2015年第23期21卷 7305-7312页

作者： Francisco Hernández-Bernal Georgina Castellanos-Sierra Carmen M Valenzuela-Silva Karem M Catasús-Alvarez Osmany Martínez-Serrano Odalys C Lazo-Diago Cimara H Bermúdez-Badell José R Causa-García juan e domínguez-suárez Pedro A López-Saura 无 Clinical Research Direction Center for Genetic Engineering and Biotechnology juan B.Zayas Hospital ernesto Guevara Hospital Celia Sánchez Hospital Manzanillo Gustavo Aldereguía Hospital THeReSA-4(Treatment of Hemorrhoids with Recombinant Streptokinase Application)Group of Investigators

AIM: To compare the efficacy and safety of recombinant streptokinase(rSK) vs hydrocortisone acetate-based suppositories in acute hemorrhoidal ***: A multicenter(11 sites), randomized(1:1:1), open, controlled trial with parallel groups was performed. All participating patients gave their written,informed consent. After inclusion, patients with acute symptoms of hemorrhoids were centrally randomized to receive, as outpatients, by the rectal route, suppositories of rSK 200000 IU of one unit every 8 h(first 3 units)and afterwards every 12 h until 8 administrations were completed(schedule A), one unit every 8 h until 6 units were completed(schedule B), or 25 mg hydrocortisone acetate once every 8 h up to a maximum of 24 administrations. evaluations were performed at 3, 5,and 10 d post-inclusion. The main end-point was the 5thday response(disappearance of pain and bleeding, and≥ 70% reduction of the lesion size). Time to response and need for thrombectomy were secondary efficacy variables. Adverse events were also ***: Groups were homogeneous with regards to demographic and baseline characteristics. Fifth day complete response rates were 156/170(91.8%; 95%CI:87.3-96.2), 155/170(91.2%; 95%CI: 86.6%-95.7%),and 46/170(27.1%; 95%CI: 20.1%-34.0%) with rSK(schedule A and B) and hydrocortisone acetate suppositories, respectively. These 64.6% and 63.9%differences(95%CI: 56.7%-72.2% and 55.7%-72.0%)were highly significant(P e was detected since the early 3rd day evaluation(68.8% and64.1% vs 7.1% for the rSK and active control groups,respectively; P ed even at the late 10 th day assessment(97.1% and 93.5% vs67.1% for rSK and hydrocortisone acetate, respectively;P e to response was 3 d(95%CI: 2.9-3.1)for both rSK groups and 10 d(95%CI: 9.3-10.7) in the hydrocortisone acetate group. This difference was highly significant(P subgroup stratified analyses(with or without thrombosis and he

关键词： Streptokinase suppository Hydrocortisoneacetate Thrombolysis Hemorrhoids Randomizedclinical trial

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Present and future cell therapies for pancreatic beta cell replenishment

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World Journal of Gastroenterology 2012年第47期18卷 6876-6884页

作者： juan domínguez-Bendala Camillo Ricordi Diabetes Research Institute University of Miami Leonard M.Miller School of MedicineMiamiFL 33136United States Department of surgery University of Miami Miller School of MedicineMiamiFL 33136United States

If only at a small scale,islet transplantation has successfully addressed what ought to be the primary endpoint of any cell therapy:the functional replenishment of damaged tissue in *** years of less-thanoptimal approaches to immunosuppression,recent advances consistently yield long-term graft survival rates comparable to those of whole pancreas *** organ availability is the main hurdle that stands in the way of the widespread clinical utilization of this pioneering *** in stem cell research over the past decade,coupled with our decades-long experience with islet transplantation,is shaping the future of cell therapies for the treatment of *** we review the most promising avenues of research aimed at generating an inexhaustible supply of insulin-producing cells for islet regeneration,including the differentiation of pluripotent and multipotent stem cells of embryonic and adult origin along the beta cell lineage and the direct reprogramming of non-endocrine tissues into insulin-producing cells.

关键词： Human embryonic stem cells Induced pluripotent stem cells Mesenchymal stem cells Beta cell differentiation Reprogramming Islet transplantation

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The detrimental effects of lipopolysaccharideinduced neuroinflammation on adult hippocampal neurogenesis depend on the duration of the pro-inflammatory response

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Neural Regeneration Research 2019年第5期14卷 817-825页

作者： Martha Pérez-domínguez evangelina ávila-Mu?oz eduardo domínguez-Rivas Angélica Zepeda Departamento de Medicina Genómica y Toxicología Ambiental Instituto de Investigaciones Biomédicas.Universidad Nacional Autónoma de MéxicoCDMX

Adult hippocampal neurogenesis is a finely tuned process regulated by extrinsic factors. Neuroinflammation is a hallmark of several pathological conditions underlying dysregulation of neurogenesis. In animal models, lipopolysaccharide(LPS)-induced neuroinflammation leads to a neurogenic decrease mainly associated to the early inflammatory response. However, it is not well understood how the neuroinflammatory response progresses over time and if neurogenesis continues to be diminished during the late neuroinflammatory response. Moreover, it is unknown if repeated intermittent administration of LPS along time induces a greater reduction in neurogenesis. We administered one single intraperitoneal injection of LPS or saline or four repeated injections(one per week) of LPS or saline to young-adult mice. A cohort of new cells was labeled with three 5-bromo-2-deoxyuridine injections(one per day) 4 days after the last LPS injection. We evaluated systemic and neuroinflammation-associated parameters and compared the effects of the late neuroinflammatory response on neurogenesis induced by each protocol. Our results show that 1) a single LPS injection leads to a late pro-inflammatory response characterized by microglial activation, moderate astrocytic reaction and increased interleukin-6 levels. This response correlates in time with decreased neurogenesis and 2) a repeated intermittent injection of LPS does not elicit a late pro-inflammatory response although activated microglia persists. The latter profile is not accompanied by a continued longterm hippocampal neurogenic decrease. Hereby, we provide evidence that the neuroinflammatory response is a dynamic process that progresses in a milieu-dependent manner and does not necessarily lead to a neurogenic decrease, highlighting the complex interaction between the immune system and neurogenesis.

关键词： dentate gyrus subgranular zone inflammation microglia astrocytes IL-6 cytokines cell proliferation neural progenitor cells immature neurons long-term short-term adult hippocampal neurogenesis

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Impact of chitosan-based nanocarriers on cytoskeleton dynamics:Current status and challenges

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BIOCeLL 2022年第4期46卷 885-891页

作者： M.CAROLINA DI SANTO AGUSTINA ALAIMO ANA PAULA domÍnguez RUBIO OSCAR e.PÉrez Departamento de Química Biológica Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina Instituto de Química Biológica de la Facultad de Ciencias exactas y Naturales Consejo Nacional de Investigaciones Científicas y Técnicas(IQUIBICENCONICET)Buenos AiresArgentina

Chitosan-based nanocarriers(CS-NCs)show a promising role in improving drugs and bioactive compounds delivery for ***,the effects exerted by CS-NCs at the cellular level,including their recognition and uptake,have not been fully investigated *** factors,including size,shape,concentration,and surface chemistry of CS-NCs,play an important role in determining the types of intracellular signals *** mechanism of uptake and the involvement of the cytoskeleton during the CS-NCs endocytosis variates among the different cell types as well as further effects observed inside *** the present work,we discuss the effects induced by CS-NCs per se on the cytoskeleton,a key component in cell architecture and *** focus of this report is made on tumoral and normal biological models in which CS-NCs could differentially affect the cell *** recent years reports regarding the impact of CS-NCs on cytoskeleton dynamics and the current techniques for its evaluation are summarized and *** mechanisms underlying cytoskeletal impact after cell exposure to CS-NCs is critical for the design of safest value-added formulations in the biomedical ***,this revision points out some interesting aspects of cytoskeletal changes and cell death encompassing anti-tumoral effects.

关键词： Cytoskeletal dynamics Cellular uptake endocytosis Nanocarriers Chitosan Bioactive compounds

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Taking advantage of the potential of mesenchymal stromal cells in liver regeneration:Cells and extracellular vesicles as therapeutic strategies

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World Journal of Gastroenterology 2018年第23期24卷 2427-2440页

作者： esteban juan Fiore Luciana María domínguez juan Bayo Mariana Gabriela García Guillermo Daniel Mazzolini Laboratory of Gene Therapy Instituto de Investigaciones en Medicina TraslacionalCONICET-Universidad Austral

Cell-based therapies for acute and chronic liver diseases are under continuous progress. Mesenchymal stem/stromal cells(MSCs) are multipotent cells able to migrate selectively to damaged tissue and contribute to its healing and regeneration. The MSC pro-regenerative effect occurs due to their immunomodulatory capacity and their ability to produce factors that promote cell protection and survival. Likewise,it has been observed that part of their paracrine effect is mediated by MSC-derived extracellular vesicles(eVs). eVs contain proteins,lipids and nucleic acids(DNA,m RNA,mi RNA,lnc RNA) from the cell of origin,allowing for intercellular communication. Recently,different studies have demonstrated that MSC-derived eVs could reproduce,at least in part,the biological effects obtained by MSCbased therapies. Moreover,due to eVs' stability for long periods of time and easy isolation methods they have become a therapeutic option to MSCs treatments. This review summarizes the latest results achieved in clinical trials using MSCs as cell therapy for liver regeneration,the role of eVs in liver physiopathology and the potential of MSC-derived eVs as intercellular mediators and therapeutic tools in liver diseases.

关键词： Mesenchymal stem cells extracellular vesicles Cirrhosis Liver Acute damage Regeneration

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Whole lesion histogram analysis of apparent diffusion coefficient predicts therapy response in locally advanced rectal cancer

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World Journal of Gastroenterology 2022年第23期28卷 2609-2624页

作者： Mayra evelia Jiménez de los Santos juan Armando Reyes-Pérez Victor domínguez Osorio Yolanda Villaseñor-Navarro Liliana Moreno-Astudillo Itzel Vela-Sarmiento Isabel Sollozo-Dupont Department of Radiology National Cancer InstituteMexico 14080Mexico Department of Gastrointestinal surgery National Cancer InstituteMexico 14080Mexico

BACKGROUND Whole-tumor apparent diffusion coefficient(ADC)histogram analysis is relevant to predicting the neoadjuvant chemoradiation therapy(nCRT)response in patients with locally advanced rectal cancer(LARC).AIM To evaluate the performance of ADC histogram-derived parameters for predicting the outcomes of patients with *** This is a single-center,retrospective study,which included 48 patients with *** patients underwent a pre-treatment magnetic resonance imaging(MRI)scan for primary tumor staging and a second restaging MRI for response *** sample was distributed as follows:18 responder patients(R)and 30 non-responders(non-R).eight parameters derived from the whole-lesion histogram analysis(ADCmean,skewness,kurtosis,and ADC10^(th),25^(th),50^(th),75^(th),90^(th) percentiles),as well as the ADCmean from the hot spot region of interest(ROI),were calculated for each patient before and after *** all data were compared between R and non-R using the Mann-Whitney U *** measures of diagnostic accuracy were applied:the receiver operating characteristic curve and the diagnostic odds ratio(DOR).We also reported intra-and interobserver variability by calculating the intraclass correlation coefficient(ICC).ResuLTS Post-nCRT kurtosis,as well as post-nCRT skewness,were significantly lower in R than in non-R(both Pespectively).We also found that,after treatment,R had a larger loss of both kurtosis and skewness than non-R(Δ%kurtosis and Δ skewness,Per parameters that demonstrated changes between groups were post-nCRT ADC10^(th),Δ%ADC10^(th),Δ%ADCmean,and ROIΔ%***,the best diagnostic performance was achieved byΔ%kurtosis at a threshold of 11.85%(Area under the receiver operating characteristic curve[AUC]=0.991,DOR=376),followed by post-nCRT kurtosis=0.78×10^(-3)mm^(2)/s(AUC=0.985,DOR=375.3),Δskewness=0.16(AUC=0.885,DOR=192.2)and post-nCRT skewness=1.59×10^(-3)mm^(2)/s(AUC=0.815,DOR=168.6).Finally,intraclass corr

关键词： Apparent diffusion coefficient Diffusion-weighted imaging Histogram analysis Magnetic resonance imaging Locally advanced rectal cancer

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Usefulness of duodenal biopsy during routine upper gastrointestinal endoscopy for diagnosis of celiac disease

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World Journal of Gastroenterology 2006年第31期12卷 5028-5032页

作者： S Riestra F domínguez e Fernández-Ruiz e García-Riesco R Nieto e Fernández L Rodrigo Gastroenterology Section and Gastroenterology ServiceHospital Central de Asturias Spain Gastroenterology Section and Pathology ServiceHospital Valle del Nalón Spain Gastroenterology Section and Biochemistry ServiceHospital de Cabuenes Spain

AIM： To describe the trend in duodenal biopsy performance during routine upper gastrointestinal endoscopy in an adult Spanish population, and to analyze its value for the diagnosis of celiac disease in clinical practice. MeTHODS： A 15 year-trend （1990 to 2004） in duodenal biopsy performed when undertaking upper gastrointestinal endoscopy was studied. We analysed the prevalence of celiac disease in the overall group, and in the subgroups with anaemia and/or chronic diarrhoea. ResuLTS： Duodenal biopsy was performed in 1033 of 13 678 upper gastrointestinal endoscopies （7.6%）; an increase in the use of such was observed over the study period （1.9% in 1990-1994, 5% in 1995-1999 and 12.8% in 2000-2004）. Celiac disease was diagnosed in 22 patients （2.2%）, this being more frequent in women than in men （3% and 1% respectively）. Fourteen out of 514 （2.7%） patients with anaemia, 12 out of 141 （8.5%） with chronic diarrhoea and 8 out of 42 （19%） with anaemia plus chronic diarrhoea had celiac disease. A classical clinical presentation was observed in 55% of the cases, 23% of the patients had associated dermatitis herpetiformis and 64% presented anaemia; 9% were diagnosed by familial screening and 5% by cryptogenetic hypertransaminasaemia. CONCLUSION： Duodenal biopsy undertaken during routine upper gastrointestinal endoscopy in adults, has been gradually incorporated into clinical practice, and is a useful tool for the diagnosis of celiac disease in high risk groups such as those with anaemia and/or chronic diarrhoea.

关键词： Duodenal biopsy Upper gastrointestinal endoscopy Celiac disease Anemia Chronic diarrhea

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Spatiotemporal variation in the adult sex ratio,male aggregation,and movement of two tropical cloud forest dung beetles

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Current Zoology 2022年第6期68卷 635-644页

作者： Julliana BARReTTO Martha L.BAeNA Israel Huesca domınguez Federico eSCOBAR Red de ecoetologıa Instituto de EcologıaXalapaC.P.91073Mexico Instituto de Investigaciones Biologicas Universidad Veracruzana.Av.Luis Castelazo Ayala s/n Col.IndustrialAnimasXalapaC.P.91190Mexico

While theory suggests that at conception the sex ratio should be balanced(1:1),this can be variable across space and time in wild ***,studies of the environmental factors that regulate adult sex ratio(ASR)in species with different life history traits are *** capture–recapture over a year,we analyzed the influence of habitat type(forest and nonforest)and season(rainy and dry)on variation in ASR,male aggregation and the trajectory movement of 2 dung beetle species with different life history traits:Deltochilum mexicanum(a hornless roller species)and Dichotomius satanas(a tunneler species with horns on its head and thorax).We found opposite *** *** population tends to be female-biased,but the population of *** tends to be predominantly male,and observed values were not related to habitat type or ***,the 95%confidence intervals estimated were highly variable between seasons depending on *** examining the monthly variation in ASR for both habitats,we found that it depends on the *** addition,male aggregation differed between species depending on habitat type and season,and species movement patterns were closely related to their habitat *** on our results,we argue that comparative population studies of species with different life history traits are necessary to understand the variation in demographic parameters as well as its ecological and evolutionary implications in the face of spatial and climatic environmental variation.

关键词： habitat preference mark-recapture mean crowding seasonality Scarabaeinae sex proportion

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Heterologous expression of bacteriocin e-760 in Chlamydomonas reinhardtii and functional analysis

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Phyton-International Journal of experimental Botany 2019年第1期88卷 25-35页

作者： Quezada-Rivera JJ Re Soria-Guerra FS Pérez-Juárez L Martínez-González Se Valdés-Rodríguez NL Vasco-Méndez JF Morales-domínguez Departamento de Química Universidad Autónoma de AguascalientesUniversidad No.940Ciudad UniversitariaC.P.20131AguascalientesAgs.México Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíZona Universitaria PonienteC.P.78290San Luis PotosíS.L.P.México Departamento de Biotecnología y Bioquímica Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalCinvestav-Unidad Irapuato.Km.9.6 Libramiento Norte Carr.Irapuato-León 36824 IrapuatoGto.México

The use of antimicrobial peptides(AMPs)synthesized by bacteria(bacteriocins)is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass *** bacteriocin e-760 isolated from the genus enterococcus *** been shown to possess inhibitory activity against Gram-negative and Gram-positive *** this study,the expression of a chimeric protein coding for e-760 in the nucleus of *** was evaluated,as well as,its antibacterial *** synthetic gene e-760S was inserted into the genome of *** using Agrobacterium tumefaciens.A transgenic line was identified in TAP medium with hygromycin and also by *** increment in the culture medium temperature of the transgenic strain at 35°C for 10 minutes,increased the production level of the recombinant protein from 0.14(Noninduced culture,NIC)to 0.36%(Induced culture,IC)of total soluble proteins(TSP);this was quantified by an eLISA *** e-760 possesses activity against Staphylococcus aureus in 0.34 U log,Streptococcus agalactiae in 0.48 U log,enterococcus faecium in 0.36 U log,Pseudomonas aeruginosa in 2 U log and for Klebsiella pneumoniae,the activity was 0.07 U *** results demonstrate that the nucleus transformation of *** can function as a stable expression platform for the production of the synthetic gene e-760 and it can potentially be used as an antibacterial agent.

关键词： Heterologous expression Antibacterial activity Bacteriocin e-760 Chlamydomonas reinhardtii Log inactivation Nuclear transformation

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Nanoscale mapping of optically inaccessible bound-states-in-the-continuum

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Light(Science & Applications) 2022年第2期11卷 188-196页

作者： Zhaogang Dong Zackaria Mahfoud Ramón Paniagua-domínguez Hongtao Wang Antonio I.Fernández-domínguez Sergey Gorelik Son Tung Ha Febiana Tjiptoharsono Arseniy I.Kuznetsov Michel Bosman Joel K.W.Yang Institute of Materials Research and engineering A*STAR(Agency for ScienceTechnology and Research)2 Fusionopolis Way#08-03 Innovis138634 SingaporeSingapore Department of Materials Science and engineering National University of Singapore9 Engineering Drive 1117575 SingaporeSingapore Singapore University of Technology and Design 8 Somapah Road487372 SingaporeSingapore Departamento de Física Teórica de la Materia Condensada and Condensed Matter Physics Center Universidad Autónoma de Madrid28049 MadridSpain Singapore Institute of Food and Biotechnology Innovation A*STAR(Agency for ScienceTechnology and Research)31 Biopolis Way#01-02 Nanos138669 SingaporeSingapore

Bound-states-in-the-continuum(BIC)is an emerging concept in nanophotonics with potential impact in applications,such as hyperspectral imaging,mirror-less lasing,and nonlinear harmonic *** true BIC modes are nonradiative,they cannot be excited by using propagating light to investigate their optical *** this paper,for the 1st time,we map out the strong near-field localization of the true BIC resonance on arrays of silicon nanoantennas,via electron energy loss spectroscopy with a sub-1-nm electron *** systematically breaking the designed antenna symmetry,emissive quasi-BIC resonances become *** gives a unique experimental tool to determine the coherent interaction length,which we show to require at least six neighboring antenna *** importantly,we demonstrate that quasi-BIC resonances are able to enhance localized light emission via the Purcell effect by at least 60 times,as compared to unpatterned *** work is expected to enable practical applications of designed,ultra-compact BIC antennas such as for the controlled,localized excitation of quantum emitters.

关键词： antenna states mapping

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